Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-04T21:03:38.818Z Has data issue: false hasContentIssue false
  • English
  • Français

Serum lipid peroxidation markers are correlated with those in brain samples in different stress models

Published online by Cambridge University Press:  11 July 2013

Asuman Celikbilek ,
Ayse Yesim Gocmen ,
Nermin Tanik ,
Nazmi Yaras ,
Piraye Yargicoglu  and
Saadet Gumuslu
Asuman Celikbilek
Affiliation:
Department of Neurology
Ayse Yesim Gocmen
Affiliation:
Department of Biochemistry, Medical School, Bozok University, Yozgat, Turkey
Nermin Tanik
Affiliation:
Department of Neurology
Nazmi Yaras
Affiliation:
Department of Biophysics
Piraye Yargicoglu
Affiliation:
Department of Biophysics
Saadet Gumuslu*
Affiliation:
Department of Biochemistry, Medical School, Akdeniz University, Antalya, Turkey
*
Saadet Gumuslu, Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey. Tel: +00 (90) 242 2496896; Fax: +00 (90) 242 2274495; E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective

Stress can stimulate increased production of oxygen radicals. We investigated the correlations between serum levels of lipid peroxidation markers and those in brain samples in different stress models.

Methods

Animals (n = 96) were divided equally into eight groups: a control group and groups treated with vitamin E (Vit E); exposed to immobilisation stress; exposed to immobilisation stress and treated with Vit E; exposed to cold stress; exposed to cold stress and treated with Vit E; exposed to both immobilisation and cold stress; and a final group exposed to both immobilisation and cold stress and treated with Vit E. Thiobarbituric acid-reactive substance (TBARS) in brain samples and levels of TBARS, corticosterone, conjugated dienes (CD), lipids, and paraoxonase-1 (PON1) activity in serum were analysed.

Results

Serum corticosterone (p < 0.001), CD (p < 0.05), lipid (p < 0.05) levels, and brain TBARS (p < 0.05) levels were significantly higher in all stress groups than in controls, and the elevated levels were reversed in the Vit E-treated stress groups (p < 0.05). Serum PON1 activity was not different among the groups (p > 0.05). Serum TBARS levels increased significantly in all stress groups (p < 0.05), but this elevation was only reversed in the group exposed to both immobilisation and cold stress and treated with Vit E (p < 0.001).

Conclusion

These results suggest that serum levels of lipid peroxidation markers can be determined readily and may be useful as indicators to evaluate the effects of oxidative stress in the brain.

Keywords

lipid peroxidationoxidative stressoxygen radicalsvitamin E
Type
Original Articles
Information
Acta Neuropsychiatrica , Volume 26 , Issue 1 , February 2014 , pp. 51 - 57
Copyright
Copyright © Scandinavian College of Neuropsychopharmacology 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Kovacs, P, Juranek, I, Stankovicova, T, Svec, P. Lipid peroxidation during acute stress. Pharmazie 1996;51:51–53.Google ScholarPubMed
2.Hacioglu, G, Agar, A, Yargicoglu, P. The role of docosahexaenoic acid on visual evoked potentials in one kidney-one clip hypertension. Acta Ophthalmol Scand 2006;84:488–494.Google Scholar
3.Yaras, N, Yargicoglu, P, Agar, A, Gumuslu, S, Abidin, I, Ozdemir, S. Effect of immobilization and cold stress on visual evoked potentials. Int J Neurosci 2003;113:1055–1067.Google Scholar
4.Halliday, AM, McDonald, WI, Mushin, J. Delayed visual evoked response in optic neuritis. Lancet 1972;1:982–985.Google Scholar
5.Sahin, E, Gumuslu, S. Alterations in brain antioxidant status, protein oxidation and lipid peroxidation in response to different stress models. Behav Brain Res 2004;155:241–248.Google Scholar
6.Sahin, E, Gumuslu, S. Cold-stress-induced modulation of antioxidant defence: role of stressed conditions in tissue injury followed by protein oxidation and lipid peroxidation. Int J Biometeorol 2004;48:165–171.Google Scholar
7.Sahin, E, Gumuslu, S. Immobilization stress in rat tissues: alterations in protein oxidation, lipid peroxidation and antioxidant defense system. Comp Biochem Physiol C Toxicol Pharmacol 2007;144:342–347.Google Scholar
8.Yargicoglu, P, Yaras, N, Agar, A, Gumuslu, S, Bilmen, S, Ozkaya, G. The effect of vitamin E on stress-induced changes in visual evoked potentials (VEPs) in rats exposed to different experimental stress models. Acta Ophthalmol Scand 2003;81:181–187.Google Scholar
9.Finlay, JM, Jedema, HP, Rabinovic, AD, Mana, MJ, Zigmon, MJ, Sved, AF. Impact of corticotropin-releasing hormone on extracellular norephinephrine in prefrontal after chronic cold stress. J Neurochem 1997;69:144–150.Google Scholar
10.Inoue, T, Koyoma, T, Muraki, A, Yamashita, I. Effects of single and repeated immobilization stress on corticotropin-releasing factor concentrations in discrete rat brain regions. Prog Neuropsychopharmacol Biol Psychiatry 1993;17:161–170.Google Scholar
11.Hacioglu, G, Agar, A, Ozkaya, G, Yargicoglu, P, Gumuslu, S. The effect of different hypertension models on visual evoked potentials. Int J Neurosci 2002;112:1321–1335.CrossRefGoogle ScholarPubMed
12.Hetzler, BE, Boyes, WK, Creason, J, Dyer, RS. Temperature dependent changes in visual evoked potentials of rats. Electroenceph Clin Neurophysiol 1988;70:137–154.Google Scholar
13.Wasowicz, W, Nève, J, Peretz, A. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem 1993;39:2522–2526.Google Scholar
14.Recknagel, RO, Glende, EA Jr. Spectrophotometric detection of lipid conjugated dienes. Methods Enzymol 1984;105:331–337.Google Scholar
15.Friedewald, WT, Levy, RI, Fredrickson, DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499–502.CrossRefGoogle ScholarPubMed
16.Gocmen, AY, Gumuslu, S, Semiz, E. Association between paraoxonase-1 activity and lipid peroxidation indicator levels in people living in the Antalya region with angiographically documented coronary artery disease. Clin Cardiol 2004;27:426–430.Google Scholar
17.Pilcher, JJ, Nadler, E, Busch, C. Effects of hot and cold temperature exposure on performance: a meta-analytic review. Ergonomics 2002;45:682–698.Google Scholar
18.Hong, SC, Zhao, SP, Wu, ZH. Probucol up-regulates paraoxonase 1 expression in hepatocytes of hypercholesterolemic rabbits. J Cardiovasc Pharmacol 2006;47:77–81.Google Scholar
19.Silambarasan, T, Raja, B. Diosmin, a bioflavonoid reverses alterations in blood pressure, nitric oxide, lipid peroxides and antioxidant status in DOCA-salt induced hypertensive rats. Eur J Pharmacol 2012;679:81–89.Google Scholar
20.Matsumoto, K, Yobimoto, K, Huong, NT, Abdel-Fattah, M, Van Hien, T, Watanabe, H. Psychological stress-induced enhancement of brain lipid peroxidation via nitric oxide systems and its modulation by anxiolytic and anxiogenic drugs in mice. Brain Res 1999;839:74–84.Google Scholar
21.Siu, AW, Reiter, RJ, To, CH. Pineal indoleamines and vitamin E reduce nitric oxide-induced lipid peroxidation in rat retinal homogenates. J Pineal Res 1999;27:122–128.Google Scholar
22.Gądek-Michalska, A, Tadeusz, J, Rachwalska, P, Spyrka, J, Bugajski, J. Effect of prior stress on interleukin-1β and HPA axis responses to acute stress. Pharmacol Rep 2011;63:1393–1403.Google Scholar
23.García, A, Martí, O, Vallès, A, Dal-Zotto, S, Armario, A. Recovery of the hypothalamic–pituitary–adrenal response to stress. Effect of stress intensity, stress duration and previous stress exposure. Neuroendocrinology 2000;72:114–125.Google Scholar