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Hypothalamic digoxin and brain function

Published online by Cambridge University Press:  24 June 2014

R. K. Kurup*
Affiliation:
Department of Neurology, Medical College Hospital, Trivandrum, Kerala
P. A. Kurup
Affiliation:
Metabolic Disorders Research Center, Trivandrum, Kerala, India
*
Gouri Sadan, T.C.4/1525, North of Cliff House, Kattu Road, Kowdiar PO, Trivandrum, Kerala, India. Tel: 0471 541607; Fax: 91 0471-550782; E-mail: [email protected]

Abstract

Background and objectives:

The study assessed the biochemical differences between right hemispheric-dominant and left hemispheric-dominant individuals. The chemical hemispheric-dominance in various systemic and neuropsychiatric diseases was also studied.

Methods:

The isoprenoid metabolites, digoxin, dolichol and ubiquinone, glycoconjugate metabolism, free radical metabolism and the RBC membrane composition, were studied in individuals with differing hemispheric-dominance. The digoxin levels and RBC membrane Na+-K+ATPase activity were also studied in systemic and neuropsychiatric diseases.

Results:

The results showed that right hemispheric-dominant individuals had elevated digoxin levels, increased free radical production and reduced scavenging, increased tryptophan catabolites and reduced tyrosine catabolites, increased glycoconjugate levels and increased cholesterol : phospholipid ratio of RBC membranes. Left hemispheric-dominant individuals had the opposite patterns. This patterns could be correlated with various systemic and neuropsychiatric diseases.

Conclusion:

Right hemispheric-dominance represents a hyperdigoxinaemic state with membrane sodium–potassium ATPase inhibition. Left hemispheric-dominance represents the reverse pattern with hypodigoxinaemia and membrane sodium–potassium ATPase stimulation. Hemispheric-dominance could predispose to various systemic and neuropsychiatric diseases.

Type
Research Article
Copyright
Copyright © 2003 Blackwell Munksgaard

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References

Geschwind, N, Galaburda, AM. Cerebral lateralization. Cambridge, MA: MIT Press, 1987. Google Scholar
Geschwind, N, Behan, P. Left-handedness: association with immune diseases, migraine, and developmental learning disorders. Proc Natl Acad Sci USA 1982;79: 50975100.CrossRefGoogle Scholar
Goldstein, JL, Brown, MS. Regulation of the mevalonate pathway. Nature 1990;343: 425430.CrossRefGoogle ScholarPubMed
Jyothi, JK. Investigations on metabolic derangement in coronary artery disease and neurodegenerative disorders. Trivandrum: Kerala University Press 1998. Google Scholar
Rao, AV, Ramakrishnan, S. Estimation of HMG CoA reductase activity. Clin Chem 1975;21: 15231528.CrossRefGoogle Scholar
Wallach, DFH, Kamath, VB. Methods in enzymology. New York: Academic Press, 1966. Google Scholar
Arun, P, Ravikumar, A, Leelamma, S, Kurup, PA. Identification and estimation of endogenous digoxin in biological fluids and tissues by TLC and HPLC. Indian J Biochem Biophys 1998;35: 308312.Google ScholarPubMed
Palmer, DN, Maureen, AA, Robert, DJ. Separation of some neutral lipids by normal phase high performance liquid chromatography on a cyanopropul column ubiquinone, dolichol and choleterol levels in sheep liver. Anal Biochem 1984;140: 315319.CrossRefGoogle Scholar
Price, WJ. Spectrochemical analysis by atomic absorption. New York: John Wiley, 1985Google Scholar
Bloxam, DL, Warren, WH. Error in the determination of tryptophan by the method of Denkala and Dewey. A revised procedure. Anal Biochem 1974;60: 621625.CrossRefGoogle Scholar
Wong, PWK, O'Flynn, ME, Inouye R. Flourimetric method for tyrosine. Clin Chem 1964;10: 10981100. CrossRefGoogle Scholar
Curzon, G, Green, AR. Rapid method for the determination of 5-hydroxy tryptophan and 5-hydroxy indoleacetic acid in certain regions of rat brain. Br J Pharmacol 1979;39: 653655. CrossRefGoogle Scholar
Well-Malherbe. Methods of biochemical analysis. New York; Inter Science, 1971. Google Scholar
Arun, P, Ravi Kumar, A, Leelamma, S, Kurup, PA. Endogenous alkaloids in the brain of rats loaded with tyrosine/tryptophan in the serum of patients of neurodegenerative and psychiatric disorders. Ind J Med Res 1998;107: 231238. Google Scholar
Manoj, AJ, Kurup, PA. Changes in the glycosaminoglycans and glycoproteins in the rat brain during protein calorie malnutrition. Clin Biochem Nutr 1998; 25: 149157. Google Scholar
Lowenstein, JM. Methods in enzymology, 25. New York: Academic Press, 1969. Google Scholar
Kakkar, P, Das, B, Viswanathan, PN. A modified spectrophotometric assay of SOD. Indian J Biochem Biophys 1984;21: 130.Google Scholar
Maehly, AC, Chance, B. The assay of catalase and peroxidase. Meth Biochem Anal 1954;2: 357. CrossRefGoogle Scholar
Paglia, DE, Valentine, WN. Studies on quantitative and qualitative characterisation of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70: 158Google ScholarPubMed
Horn, HD, Burns, FH. Methods of enzymatic analysis. New York: Academic Press, 1978. Google Scholar
Will, ED. Lipid peroxide formation in microsomes — general consideration. Biochem J 1969;113: 315CrossRefGoogle Scholar
Brien, PJO. Estimation of conjugated dienes and hydroperoxide. Can J Biochem 1969;47: 485.Google Scholar
Beutler, E, Duran, O, Kelley, BM. Modified procedure for the estimation reduced glutathione. J Lab Clin Med 1963;61: 882. Google Scholar
Gabor, G, Allon, N. Spectrofluorometric method for NO determination. Anal Biochem 1994;220: 16CrossRefGoogle ScholarPubMed
Haga, H. Effects of dietary magnesium supplementation on diurnal variation of BP and plasma Na+-K+ATPase activity in essential hypertension. Jpn Heart J 1992;33: 785798.CrossRefGoogle Scholar
Ravi Kumar, A, Jyothi, A, Kurup, PA. 14C-acetate incorporation into digoxin in rat rain and effect of digoxin administration. Indian J Exp Biol 2001;3: 420426Google Scholar
Hisaka, A, Kasamatu, S, Takenaga, N. Absorption of a novel prodrug of DOPA. Drug-Metab Disposal 1990;18: 621625. Google ScholarPubMed
Carpenter, WT Jr,Buchanan, RW. Medical progress in schizophrenia. N Engl J Med 1994;30: 681690. CrossRefGoogle Scholar
Greenamyre, JT, Poter, RHP. Anatomy and physiology of glutamate in CNS. Neurology 1994;44 (Suppl. 8): S7S13.Google ScholarPubMed
Rothstein, JD, Kunel, R, Choudhary, Vet al. Excitatory amino acids and ALS, an update. Ann Neurol 1991;30: 224225.CrossRefGoogle Scholar
Vinken, PJ, Bruyn, W. Handbook of clinical neurology. Amsterdam: Elsevier, 1974. Google Scholar
Bloom, FE, Kupfer, DJ. Psychopharmacology. Fourth generation of progress. New York: Raven Press, 1995. Google Scholar
Stefano, GB, Scharrer, B. Endogenous morphine and related opiates, a new class of chemical messengers. Adv Neuroimmunol 1994;4: 5769.CrossRefGoogle ScholarPubMed
Jaya, P, Kurup, PA. Effect of magnesium deficiency on the metabolism of glycosaminoglycans in rats. J Biosci 1986;10: 487497. CrossRefGoogle Scholar
Shoulson, I. Neurodegeneration. Science 1998;282: 10721074.CrossRefGoogle Scholar
Beyreuther, K, Masters, CL. Alzheimer's disease — tangle disentanglement. Nature 1996; 383: 476477.CrossRefGoogle ScholarPubMed
Slegel, RA. A lesson from secretory granules. Nature 1998;394: 428429. Google Scholar
Ploegh, HL. Viral strategies for immune evasion. Science 1998;280: 248253.CrossRefGoogle ScholarPubMed
Martin, R, McFarland, HF. Editorial. Ann Neurol 1995;38: 12. Google Scholar
Linstinsky, JL, Siegal, GP, Listinskyu, CM. Alpha-l-lucose a potentially critical molecule in pathologic processess including neoplasia. Am J Clin Pathol 1998;110: 425440.CrossRefGoogle Scholar
Feinmann, R, Sawyer, J, Hardin, J, Tricot, G. Cytogenetics and molecular genetics in multiple myeloma. Hematol Oncol Clin N Am 1997;11: 121. CrossRefGoogle Scholar
Wiedemann, C, Cockcroft, S. Vesicular transport. Nature 1998;394: 426428.CrossRefGoogle ScholarPubMed
Green, DR, Reed, JC. Mitochondria and apoptosis. Science 1998;281: 13091316.CrossRefGoogle ScholarPubMed
Jacob, RA. Nutrition, health and anioxidants. INFORM 1994;5: 12711275. Google Scholar
Finkel, TH. T-cell development and transmembrane signalling. Changing biological responses through an unchanging receptor. Immunol Today 1991;12: 7986.CrossRefGoogle ScholarPubMed
Ashkenazi, A, Dixit, VM. Death receptors signaling and modulation. Science 1998;281: 13051308.CrossRefGoogle ScholarPubMed
Katsuki, A, Sumida, Y, Murashima, Set al. Serum levels of TNF alpha are increased in obese patients with non-insulin dependent diabetes mellitus. J Clin Endocrinol Metab 1998;83: 859862.Google Scholar
Stefan, C, Wera, S, Stalmans, W, Bollen, M. The inhibition of insulin receptor by the receptor protein PC is not specific and result from hydrolysis of ATP. Diabetes 1996;45: 980986.CrossRefGoogle Scholar
Latha, PK, Brahmachari, SK. B to Z transitions in DNA and their. biological implications. J Scientific Industrial Res 1986;45: 521533. Google Scholar
Taubes, G. double helix does chemistry at a distance — but how ? Science 1977;275: 14201421. CrossRefGoogle Scholar