Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-22T23:28:25.023Z Has data issue: false hasContentIssue false
  • English
  • Français

Changes of ultrastructure and downregulation of heat shock protein 70 and fibroblast growth factor 2 in gastric mucosa of rats with depressive-like behaviour

Published online by Cambridge University Press:  24 June 2014

Gonglin Hou ,
Mingming Tang ,
Linlin Yan  and
Mingming Wang
Gonglin Hou*
Affiliation:
Department of psychology, Zhejiang Sci-Tech University, Hangzhou 310018, China
Mingming Tang
Affiliation:
Department of psychology, Zhejiang Sci-Tech University, Hangzhou 310018, China
Linlin Yan
Affiliation:
Department of psychology, Zhejiang Sci-Tech University, Hangzhou 310018, China
Mingming Wang
Affiliation:
Department of psychology, Zhejiang Sci-Tech University, Hangzhou 310018, China
*
Gonglin Hou, Department of psychology, Zhejiang Sci-Tech University, 579# Mailbox, Hangzhou 310018, China. Tel: +86 57186843234; Fax: +86-57188492242 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Extract

Objective: To understand the underlying mechanism of gastric symptoms in patients with depressive disorder.

Methods: This study tested in the rat depression model evoked with chronic mild stress whether the microstructure gastric mucosa is injured using scanning electronic microscopy and transmission electronic microscopy (TEM). In addition, the expression of heat shock protein 70 (HSP70) and fibroblast growth factor 2 (FGF2) proteins in the gastric mucosa were measured by Western blotting.

Results: We found that the gastric epithelial cells were ruptured and the gastric pits were widened in rats with depression. The amount of mucous granules was also reduced in the surface mucous cells. Moreover, parietal cells became active, and the secretory canaliculi were magnified. Expression of HSP70 and FGF2 was reduced in the gastric mucosa.

Conclusions: These findings suggested that gastric symptoms in rats with depressive-like behaviour were caused by the injury of the gastric mucosa, AQ1 and HSP70 and FGF2 may be key molecules in the pathogenesis.

Keywords

depressionelectronic microscopyfibroblast growth factor 2gastric mucosaheat shock protein 70ultrastructure
Type
Research Article
Information
Acta Neuropsychiatrica , Volume 24 , Issue 3 , June 2012 , pp. 160 - 165
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Budavari, AI, Olden, KW.Psychosocial aspects of functional gastrointestinal disorders. Gastroenterol Clin North Am 2003;32:477–506.CrossRefGoogle ScholarPubMed
2.Levy, RL, Olden, KW, Naliboff, BD et al. Psychosocial aspects of the functional gastrointestinal disorders. Gastroenterology 2006;130:1447–1458.CrossRefGoogle ScholarPubMed
3.Van Oudenhove, L, Aziz, Q.Recent insights on central processing and psychological processes in functional gastrointestinal disorders. Dig Liver Dis 2009;41:781–787.CrossRefGoogle ScholarPubMed
4.Barry, S, Dinan, TG.Functional dyspepsia: are psychosocial factors of relevance? World J Gastroenterol 2006;12:2701–2707.CrossRefGoogle ScholarPubMed
5.Mayer, EA, Naliboff, BD, Chang, L, Coutinho, SVV.Stress and irritable bowel syndrome. Am J Physiol Gastrointest Liver Physiol 2001;280:G519–G524.CrossRefGoogle ScholarPubMed
6.Overmier, JB, Murison, R.Anxiety and helplessness in the face of stress predisposes, precipitates, and sustains gastric ulceration. Behav Brain Res 2000;110:161–174.CrossRefGoogle ScholarPubMed
7.Kiang, JG, Tsokos, GC.Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology. Pharmacol Ther 1998;80:183–201.CrossRefGoogle ScholarPubMed
8.Esaki, M, Aoyagi, K, Matsumoto, T, Kuwano, Y, Shimizu, M, Fujishima, M.Effects of omeprazole and famotidine on fibroblast growth factor-2 during artificial gastric ulcer healing in humans. Eur J Gastroenterol Hepatol 2002;14:365–369.CrossRefGoogle ScholarPubMed
9.Willner, P.Chronic mild stress (CMS) revisited: consistency and behavioural-neurobiological concordance in the effects of CMS. Neuropsychobiology 2005;52:90–110.CrossRefGoogle ScholarPubMed
10.Mar, A, Spreekmeester, E, Rochford, J.Fluoxetine-induced increases in open-field habituation in the olfactory bulbectomized rat depend on test aversiveness but not on anxiety. Pharmacol Biochem Behav 2002;73:703–712.CrossRefGoogle Scholar
11.Brenes Saenz, JC, Villagra, OR, Fornaguera Trias, J.Factor analysis of forced swimming test, sucrose preference test and open field test on enriched, social and isolated reared rats. Behav Brain Res 2006;169:57–65.CrossRefGoogle ScholarPubMed
12.Sarkisova, KY, Kulikov, MA.Behavioral characteristics of WAG/Rij rats susceptible and non-susceptible to audiogenic seizures. Behav Brain Res 2006;166:9–18.CrossRefGoogle ScholarPubMed
13.Krishnan, V, Nestler, EJ.The molecular neurobiology of depression. Nature 2008;455:894–902.CrossRefGoogle ScholarPubMed
14.Nestler, EJ, Barrot, M, DiLeone, RJ, Eisch, AJ, Gold, SJ, Monteggia, LM.Neurobiology of depression. Neuron 2002;34:13–25.CrossRefGoogle ScholarPubMed
15.Shelton, RC.The molecular neurobiology of depression. Psychiatr Clin North Am 2007;30:1–11.CrossRefGoogle ScholarPubMed
16.Gama, P, Goldfeder, EM, de Moraes, JC, Alvares, EP.Cell proliferation and death in the gastric epithelium of developing rats after glucocorticoid treatments. Anat Rec 2000;260:213–221.3.0.CO;2-9>CrossRefGoogle ScholarPubMed
17.Tanaka, T, Kobayashi, T, Sunaga, K, Tani, S.Effect of glucocorticoid on expression of rat MUC5AC mRNA in rat gastric mucosa in vivo and in vitro. Biol Pharm Bull 2001;24:634–637.CrossRefGoogle ScholarPubMed
18.Sandu, C, Artunc, F, Grahammer, F et al. Role of the serum and glucocorticoid inducible kinase SGK1 in glucocorticoid stimulation of gastric acid secretion. Pflugers Arch 2007;455:493–503.CrossRefGoogle ScholarPubMed
19.Yenari, MA, Liu, J, Zheng, Z, Vexler, ZS, Lee, JE, Giffard, RG.Antiapoptotic and anti-inflammatory mechanisms of heat-shock protein protection. Ann N Y Acad Sci 2005;1053:74–83.Google ScholarPubMed
20.Jolly, C, Morimoto, RI.Role of the heat shock response and molecular chaperones in oncogenesis and cell death. J Natl Cancer Inst 2000;92:1564–1572.CrossRefGoogle ScholarPubMed
21.Feder, ME, Hofmann, GE.Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annu Rev Physiol 1999;61:243–282.CrossRefGoogle ScholarPubMed
22.Kumar, Y, Tatu, U.Stress protein flux during recovery from simulated ischemia: induced heat shock protein 70 confers cytoprotection by suppressing JNK activation and inhibiting apoptotic cell death. Proteomics 2003;3:513–526.CrossRefGoogle ScholarPubMed
23.Otaka, M, Odashima, M, Watanabe, S.Role of heat shock proteins (molecular chaperones) in intestinal mucosal protection. Biochem Biophys Res Commun 2006;348:1–5.CrossRefGoogle ScholarPubMed
24.Otaka, M, Odashima, M, Izumi, Y et al. Target molecules of molecular chaperone (HSP70 family) in injured gastric mucosa in vivo. Life Sci 2009;84:664–667.CrossRefGoogle ScholarPubMed
25.Otaka, M, Matsuhashi, T, Odashima, M et al. Enhancement of cytoprotective ability and cell restoration in 70-kDa heat shock protein gene-transfected rat gastric mucosal cells. Aliment Pharmacol Ther 2006;24:272–277.CrossRefGoogle Scholar
26.Luo, JC, Lin, HY, Lu, CL et al. Dexamethasone inhibits basic fibroblast growth factor-stimulated gastric epithelial cell proliferation. Biochem Pharmacol 2008;76:841–849.CrossRefGoogle ScholarPubMed
27.Hull, MA, Brough, JL, Powe, DG, Carter, GI, Jenkins, D, Hawkey, CJ.Expression of basic fibroblast growth factor in intact and ulcerated human gastric mucosa. Gut 1998;43:525–536.CrossRefGoogle ScholarPubMed
28.Kimura, T, Noda, M, Sugihara, H, Kashima, K, Hattori, T.Expression of fibroblast growth factor-2 transcripts in the healing of acetic acid-induced gastric ulcers. APMIS 1999;107:767–772.CrossRefGoogle ScholarPubMed
29.Becker, JC, Beckbauer, M, Domschke, W, Herbst, H, Pohle, T.Fibrin glue, healing of gastric mucosal injury, and expression of growth factors: results from a human in vivo study. Gastrointest Endosc 2005;61:560–567.CrossRefGoogle ScholarPubMed
30.Kato, M, Okugawa, G, Wakeno, M et al. Effect of basic fibroblast growth factor (FGF2) gene polymorphisms on SSRIs treatment response and side effects. Eur Neuropsychopharmacol 2009;19:718–725.CrossRefGoogle ScholarPubMed
31.Turner, CA, Gula, EL, Taylor, LP, Watson, SJ, Akil, H.Antidepressant-like effects of intracerebroventricular FGF2 in rats. Brain Res 2008;1224:63–68.CrossRefGoogle ScholarPubMed