In Situ Hybridisation: Application to Developmental Biology and Medicine

doi:10.1017/CBO9780511629051

In Situ Hybridisation

Application to Developmental Biology and Medicine

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This Book has been cited by the following publications. This list is generated based on data provided by Crossref.

Harris, N. 1992. Molecular histochemistry and plant biology: a review. Journal of Microscopy, Vol. 166, Issue. 1, p. 3.

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Fleming, Kenneth A. Evans, Mark Ryley, K. Claire Franklin, David Lovell‐badge, Robin H. and Morey, Adrienne L. 1992. Optimization of non‐isotopic in situ hybridization on formalin‐fixed, paraffin‐embedded material using digoxigenin‐labelled probes and transgenic tissues. The Journal of Pathology, Vol. 167, Issue. 1, p. 9.

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Moorman, Antoon F. M. De Boer, Piet A. J. Vermeulen, Jacqueline L. M. and Lamers, Wouter H. 1993. Practical aspects of radio-isotopic in situ hybridization on RNA. The Histochemical Journal, Vol. 25, Issue. 4, p. 251.

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Balzer, Hans-J�rg Borisiuk, Ljudmilla Meyer, Hans-Martin Matzk, Fritz and B�umlein, Helmut 1996. A pollen allergen-encoding gene is expressed in wheat ovaries. Plant Molecular Biology, Vol. 32, Issue. 3, p. 435.

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Edited by N. Harris, University of Durham, D. G. Wilkinson, National Institute for Medical Research, London

Publisher:: Cambridge University Press
Online publication date:: August 2010
Print publication year:: 1990
Online ISBN:: 9780511629051
DOI:: https://doi.org/10.1017/CBO9780511629051

Series:: Society for Experimental Biology Seminar Series (40)
Subjects:: Life Sciences, Cell Biology and Developmental Biology, Zoology, Molecular Biology, Biochemistry, and Structural Biology

38.99 (GBP)

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Advances in our understanding of biological mechanisms have frequently been associated with the development of techniques. In situ hybridisation is a classic case of just such an advance. The technique effectively combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues. The information gained from this has caused something of a revolution in our understanding of developmental biology, since a fundamental aspect of development is the spatial and temporal expression of genes. In addition the technique has found application in the field of medicine, providing insights into the functioning of healthy tissues and the diagnosis and study of diseases. This book brings together contributions from leaders in the application of in situ hybridisation and guides the would-be exponent through the various options and variations of the technique.

"...very useful for biologists, molecular biologists, biophysicists, and advanced students." Journal of Steroid Biochemistry and Molecular Biology

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In Situ Hybridisation

Application to Developmental Biology and Medicine

This Book has been cited by the following publications. This list is generated based on data provided by Crossref.

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Contents

Frontmatter
pp i-iv

Contents
pp v-vi

List of contributors
pp vii-x

Preface
pp xi-xii

Non-radioisotopic labels for in situ hybridisation histochemistry: a histochemist's view.
pp 1-32

Use of haptenised nucleic acid probes in fluorescent in situ hybridisation
pp 33-42

The use of complementary RNA probes for the identification and localisation of peptide messenger RNA in the diffuse neuroendocrine system
pp 43-68

Contributions of the spatial analysis of gene expression to the study of sea urchin development
pp 69-96

Advantages and limitations of in situ hybridisation as exemplified by the molecular genetic analysis of Drosophila development
pp 97-114

The use of in situ hybridisation to study the localisation of maternal mRNAs during Xenopus oogenesis
pp 115-130

In situ hybridisation in the analysis of genes with potential roles in mouse embryogenesis
pp 131-142

Evolution of algal plastids from eukaryotic endosymbionts
pp 143-156

Localisation of expression of male flower-specific genes from maize by in situ hybridisation
pp 157-174

Tissue preparation techniques for in situ hybridisation studies of storage-protein gene expression during pea seed development
pp 175-188

Investigation of gene expression during plant gametogenesis by in situ hybridisation
pp 189-204

Sexing the human conceptus by in situ hybridisation
pp 205-240

Non-isotopic in situ hybridisation in human pathology
pp 241-270

The demonstration of viral DNA in human tissues by in situ DNA hybridisation
pp 271-284

Index
pp 285-288

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In Situ Hybridisation

Application to Developmental Biology and Medicine

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