Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-09T20:25:47.086Z Has data issue: false hasContentIssue false

Chapter 6 - Temperature control in the IVF laboratory

Published online by Cambridge University Press:  05 July 2015

Kay Elder ,
Marc Van den Bergh  and
Bryan Woodward
Kay Elder
Affiliation:
Bourn Hall Clinic, Cambridge
Marc Van den Bergh
Affiliation:
Fertility Laboratory, Kantonsspital Baden
Bryan Woodward
Affiliation:
IVF Consultancy Services, Leicester
Get access

Summary

A summary is not available for this content so a preview has been provided. Please use the Get access link above for information on how to access this content.
Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
Chapter
Information
Troubleshooting and Problem-Solving in the IVF Laboratory , pp. 79 - 103
Publisher: Cambridge University Press
Print publication year: 2015

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Further reading

Biggers, JD (2001). Thoughts on embryo culture conditions. RBM Online, 4, 30–8.Google Scholar
Bureau International des Poids et Mesures, BIPM; http://www.bipm.org/en/bipm/.Google Scholar
Khabani, A, Tufts, K, Craig, L, Soules, M, Scott, L (2003). Cooling and warming rates in microdrops for embryo culture. Fertil Steril, 80, S929–3.CrossRefGoogle Scholar
Langley, MT, Marek, MA, Doody, KM, Nackley, AC, Doody, KJ (2003). Importance of micro-drop temperature measurement. EmbryoNews, 6, 3–5.Google Scholar

References

Anifandis, G (2013). Temperature variations inside commercial IVF incubators. J Assist Reprod Genet, 30, 1587–8.CrossRefGoogle ScholarPubMed
Association of Biomedical Andrologists – Laboratory Andrology Guidelines for Good Practice Version 3 (2012). Compiled & Edited by MJ Tomlinson, SJ Harbottle, BJ Woodward & KS Lindsay on behalf of the ABA. Hum Fertil, 15(4), 156–73.Google Scholar
Boone, WR, Higdon, L III (2014). Incubator management. In Culture Media, Solutions and Systems in ART, Quinn, P (ed.), Cambridge University Press, Ch. 20, pp. 269–76.Google Scholar
Bove, R (2011). Temperature measurement in the clinical laboratory. Good isn't good enough. Med Lab Obs, 43, 36–9.Google Scholar
Butler, JM, Johnson, JE, Boone, WR (2013). The heat is on: room temperature affects laboratory equipment – an observational study. JARG, 30(10), 1389–93.Google ScholarPubMed
Conaghan, J, Steel, T (2008). Real-time pH profiling of IVF culture medium using an incubator device with continuous monitoring. J Clin Embryol, 11, 15–16.Google Scholar
Cooke, S, Tyler, JPP, Driscoll, G (2002). Objective assessments of temperature maintenance using in vitro culture techniques. J Assist Reprod Genet, 19(8), 368–75.CrossRefGoogle ScholarPubMed
Edwards, JL, Hansen, PJ (1996). Elevated temperature increases heat shock protein 70 synthesis in bovine two-cell embryos and compromises function of maturing oocytes. Biol Reprod, 55(2), 341–6.Google ScholarPubMed
Fujiwara, M, Takahashi, K, Izuno, M, et al. (2007). Effect of micro-environment maintenance on embryo culture after in-vitro fertilization: comparison of top-load mini incubator and conventional front-load incubator. J Assist Reprod Genet, 24(1), 5–9.CrossRefGoogle ScholarPubMed
Grinsted, J, Kjer, JJ, Blendstrup, K, Pedersen, JF (1985). Is low temperature of the follicular fluid prior to ovulation necessary for normal oocyte development?Fertil Steril, 43(1), 34–9.CrossRefGoogle ScholarPubMed
Higdon, HL, Blackhurst, DW, Boone, WR (2007). Incubator management in an assisted reproductive technology laboratory. Fertil Steril, 89(3), 703–10.Google Scholar
Hong, K, Forman, E, Lee, H, Ferry, K, Treff, N, Scott, R (2012). Optimizing the temperature for embyro culture in IVF: a randomized controlled trial (RCT) comparing standard culture temperature of 37 °C to the reduced more physiologic temperature of 36 °C. Fertil Steril, 98, s167.CrossRefGoogle Scholar
IEC 60761 (2008). Industrial Platinum Resistance Thermometers and Platinum Temperature Sensors. International Electrotechnical Commission www.global.ihs.com/IEC_Standards.Google Scholar
Jurisicova, A, Varmuza, S, Casper, RF (1996). Programmed cell death and human embryo fragmentation. Mol Hum Reprod, 2, 93–8.CrossRefGoogle ScholarPubMed
Kelly, E, Cho, T (2014). Incubators old and new. In Culture Media, Solutions and Systems in ART, Quinn, P (ed.), Cambridge University Press, Ch. 19, pp. 258–69.Google Scholar
Kelly, PB, Deignan, KC, Emerson, G, Mocanu, E (2009). Assessment of temperature variation during in vitro culturing: a comparison of different IVF micro-environments. Fertil Steril, 92, S234.CrossRefGoogle Scholar
Leese, HJ, Baumann, CG, Brison, DR, McEvoy, TG, Sturmey, RG (2008). Metabolism of the viable mammalian embryo: quietness revisited. Mol Hum Reprod, 14(12), 667–72.CrossRefGoogle ScholarPubMed
Liebermann, J, Graham, J, Han, T, Carter, J, Tucker, M (2001). Temperature fluctuation of microdroplet in IVF. In Third Biennial Alpha Conference, 8–11 Sept. 2001, New York City, New York; Abstract book, O-12.Google Scholar
Munné, S, Magli, C, Adler, A, et al. (1997). Treatment-related chromosome abnormalities in human embryos. Hum Reprod, 12, 780–4.CrossRefGoogle ScholarPubMed
Sherbahn, R (2010). Assessment of effect of follicular fluid temperature at egg retrieval on blastocyst development, implantation and live birth rates. Fertil Steril, 94(4), S68–9.CrossRefGoogle Scholar
Stoddart, N, Rho, H, Maisel, H, Zhao, T, Tarantino, S, Fleming, S (2003). Influence of the position of the culture dish within the incubator chamber and temperature of culture media on clinical outcome following Assisted Reproductive Technology (ART) treatment. Fertil Steril, 80, s164–5.CrossRefGoogle Scholar
Sun, XF, Wang, WH, Keefe, DL (2004). Overheating is detrimental to meiotic spindles within in vitro matured human oocytes. Zygote, 12, 65–70.CrossRefGoogle ScholarPubMed
Swain, JE (2014). Decisions for the IVF laboratory: comparative analysis of embryo culture incubators. RBM Online, 28, 535–47.Google ScholarPubMed
Walker, MW, Butler, JM, Higdon, HL, Boone, WR (2013). Temperature variations within and between incubators–a prospective, observational study. J. Assist Reprod Genet, 30, 1583–5.CrossRefGoogle ScholarPubMed
Wang, WH, Meng, L, Hackett, RJ, Odenbourg, R, Keefe, DL (2001). Limited recovery of meiotic spindles in living human oocytes after cooling–rewarming observed using polarized light microscopy. Hum Reprod, 16(11), 2374–8.CrossRefGoogle ScholarPubMed
Wang, WH, Meng, L, Hackett, RJ, Oldenbourg, R, Keefe, MD (2002). Rigorous thermal control during intracytoplasmic sperm injection stabilizes the meiotic spindle and improves fertilization and pregnancy rates. Fertil Steril, 77(6), 1274–7.CrossRefGoogle ScholarPubMed
WHO (2010). Laboratory Manual for the Examination and Processing of Human Semen, Fifth edn.World Health Organization, Department of Reproductive Health and Research.Google Scholar
Yeung, QSY, Briton-Jones, CM, Tjer, GCC, Chiu, TTY, Haines, C (2004). The efficacy of test tube warming devices used during oocyte retrieval for IVF. J Assist Reprod Genet, 21, 355–60.CrossRefGoogle ScholarPubMed

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×