Oocyte retrieval is the starting point of a complex laboratory process that is the final aim of delivering a healthy baby. Exposure of oocytes to inappropriate conditions could potentially disrupt fertilization and early development. It is well accepted that minimizing stress during gamete and embryo their manipulation is critical for optimizing outcomes. The potential physicochemical stressors that can affect oocytes and embryos must be controlled to diminish possible negative effects. These include temperature control, maintaining osmolarity, pH, and protection from oxidative stress and toxic substances such as volatile organic compounds (VOC). Therefore, precautions must be taken to maintain adequate conditions to protect zygote and embryo homeostasis.