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Chapter 3 - Basic Semen Examination

Published online by Cambridge University Press:  16 February 2022

David Mortimer ,
Lars Björndahl ,
Christopher L. R. Barratt ,
José Antonio Castilla ,
Roelof Menkveld ,
Ulrik Kvist ,
Juan G. Alvarez  and
Trine B. Haugen
David Mortimer
Affiliation:
Oozoa Biomedical Inc., Vancouver
Lars Björndahl
Affiliation:
Karolinska Institutet, Stockholm
Christopher L. R. Barratt
Affiliation:
University of Dundee
José Antonio Castilla
Affiliation:
HU Virgen de las Nieves, Granada
Roelof Menkveld
Affiliation:
Stellenbosch University, South Africa
Ulrik Kvist
Affiliation:
Karolinska Institutet, Stockholm
Juan G. Alvarez
Affiliation:
Centro ANDROGEN, La Coruña
Trine B. Haugen
Affiliation:
Oslo Metropolitan University
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Summary

Provides reference methods for all aspects of a basic semen examination that are structured as standard operating procedures (SOPs) for easy use at the bench. Methods are focussed on objectivity, robustness, standardized reporting, controlling the risk of errors, and minimizing measurement uncertainty. Includes ejaculate physical characteristics, volume and pH, sperm concentration, sperm motility, sperm vitality, sperm morphology (including seminal cytology), and the simple detection of peroxidase-positive leukocytes in semen.

Keywords

semen analysisspermatozoasperm countsperm concentrationsperm motilitysperm morpholgysperm vitality
Type
Chapter
Information
A Practical Guide to Basic Laboratory Andrology , pp. 34 - 80
Publisher: Cambridge University Press
Print publication year: 2022

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