The IVF Cycle to Come: Laboratory Innovations

doi:10.1017/9781108859486.006

Chapter 5 - The IVF Cycle to Come: Laboratory Innovations

Published online by Cambridge University Press: 02 March 2020

Denny Sakkas and

Edited by

Denny Sakkas and

Alice D. Domar: Affiliation:
Boston IVF, Waltham, MA
Denny Sakkas: Affiliation:
Boston IVF, Waltham, MA
Thomas L. Toth: Affiliation:
Boston IVF, Waltham, MA

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Summary

In the first reported case of IVF and embryo development in the human, Rock and Menkin made the following description of the culture and development of a fertilized embryo: “The eggs were incubated in serum for 22.5 hours, being washed in salt solution before and after incubation, and then exposed to a washed sperm suspension in Locke’s solution for two hours at room temperature. They were again washed in Locke’s solution and cultured in fresh serum for 45 hours.

Type: Chapter
Information: Patient-Centered Assisted Reproduction
How to Integrate Exceptional Care with Cutting-Edge Technology
, pp. 54 - 66

DOI: https://doi.org/10.1017/9781108859486.006 [Opens in a new window]

Publisher: Cambridge University Press

Print publication year: 2020

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References

Menkin, MF, Rock, J In Vitro Fertilization and Cleavage of Human Ovarian Eggs. Am J Obstet Gynecol. 1948.Google Scholar

Rock, J, MM. In Vitro Fertilization and Cleavage of Human Ovarian Eggs. Science (80-). 1944; 100(2588): 105–7.Google Scholar

Ferrick, L, Lee, YSL, Gardner, DK. Reducing Time to Pregnancy and Facilitating the Birth of Healthy Children through Functional Analysis of Embryo Physiology. Biol Reprod. 2019.Google Scholar

Sati, L, Bennett, D, Janes, M, Huszar, G. Next Day Determination of Ejaculatory Sperm Motility After Overnight Shipment of Semen to Remote Locations. J Assist Reprod Genet. 2015; 32(1): 117–25.CrossRef Google Scholar PubMed

Kanakasabapathy, MK, Sadasivam, M, Singh, A, Preston, C, Thirumalaraju, P, Venkataraman, M, et al. An Automated Smartphone-Based Diagnostic Assay for Point-of-Care Semen Analysis. Sci Transl Med. 2017; 9(382).Google Scholar

Agarwal, et al. Male Oxidative Stress Infertility (MOSI): Proposed Terminology and Clinical Practice Guidelines for Management of Idiopathic Male Infertility. World J Men’s Health. 2019; 37(3): 296–312.CrossRef Google Scholar PubMed

Jenkins, TG, Aston, KI, James, ER, Carrell, DT. Sperm Epigenetics in the Study of Male Fertility, Offspring Health, and Potential Clinical Applications. Syst Biol Reprod Med. 2017; 63(2): 69–76.Google Scholar

Jodar, M, Sendler, E, Moskovtsev, SI, Librach, CL, Goodrich, R, Swanson, S, et al. Absence of Sperm RNA Elements Correlates with Idiopathic Male Infertility. Sci Transl Med. 2015; 7(295).CrossRef Google Scholar PubMed

Jodar, M, Sendler, E, Krawetz, SA. The Protein and Transcript Profiles of Human Semen. Cell Tissue Res. 2016; 363(1): 85–96.Google Scholar

Jodar, M, Selvaraju, S, Sendler, E, Diamond, MP, Krawetz, SA. The Presence, Role and Clinical Use of Spermatozoal RNAs. Hum Reprod Update. 2013; 19(6): 604–24.Google Scholar

Jenkins, TG, Aston, KI, Pflueger, C, Cairns, BR, Carrell, DT. Age-Associated Sperm DNA Methylation Alterations: Possible Implications in Offspring Disease Susceptibility. PLoS Genet. 2014; 10(7): e1004458.CrossRef Google Scholar PubMed

Carrell, DT, Aston, KI, Oliva, R, Emery, BR, De Jonge, CJ. The “Omics” of Human Male Infertility: Integrating Big Data in a Systems Biology Approach. Cell Tissue Res. 2016.Google Scholar

Khandwala, YS, Zhang, CA, Lu, Y, Eisenberg, ML. The Age of Fathers in the USA Is Rising: An Analysis of 168 867 480 Births from 1972 to 2015. Hum Reprod. 2017; 32(10): 2110–16.Google Scholar

Vaughan, DA, Sakkas, D. Sperm Selection Methods in the 21st Century. Biol Reprod.Google Scholar

de Wagenaar, B, Berendsen, JTW, Bomer, JG, Olthuis, W, van den Berg, A, Segerink, LI. Microfluidic Single Sperm Entrapment and Analysis. Lab Chip. 2015; 15(5): 1294–301.CrossRef Google Scholar PubMed

Smith, GD, Takayama, S. Application of Microfluidic Technologies to Human Assisted Reproduction. Mol Hum Reprod. 2017; gaw076.Google Scholar

Bhagwat, S, Sontakke, S, , KD, Parte, P, Jadhav, S. Chemotactic Behavior of Spermatozoa Captured Using a Microfluidic Chip. Biomicrofluidics. 2018; 12(2): 024112.Google Scholar

Ko, Y-J, Maeng, J-H, Hwang, SY, Ahn, Y. Design, Fabrication, and Testing of a Microfluidic Device for Thermotaxis and Chemotaxis Assays of Sperm. SLAS Technol. 2018; 23(6): 507–15.CrossRef Google Scholar PubMed

Eravuchira, PJ, Mirsky, SK, Barnea, I, Levi, M, Balberg, M, Shaked, NT. Individual Sperm Selection by Microfluidics Integrated with Interferometric Phase Microscopy. Methods. 2018; 136: 152–9.Google Scholar

Amaral, S, Da Costa, R, Wübbeling, F, Redmann, K, Schlatt, S. Raman Micro-Spectroscopy Analysis of Different Sperm Regions: A Species Comparison. MHR Basic Sci Reprod Med. 2018;24 (4): 185–202.Google Scholar

Weng, L, Lee, GY, Liu, J, Kapur, R, Toth, TL, Toner, M. On-Chip Oocyte Denudation from Cumulus–Oocyte Complexes for Assisted Reproductive Therapy. Lab Chip. 2018; 18(24): 3892–902.Google Scholar

Palermo, G, Joris, H, Devroey, P, Van Steirteghem, AC. Pregnancies After Intracytoplasmic Injection of Single Spermatozoon into an Oocyte. Lancet. 1992; 340(8810): 17–18.Google Scholar

Adamson, GD, de Mouzon, J, Chambers, GM, Zegers-Hochschild, F, Mansour, R, Ishihara, O, et al. International Committee for Monitoring Assisted Reproductive Technology: World Report on Assisted Reproductive Technology. Fertil Steril. 2011; 110(6): 1067–80.Google Scholar

Zhang, Z, Dai, C, Huang, JY, Wang, X, Liu, J, Ru, C, et al. Robotic Immobilization of Motile Sperm for Clinical Intracytoplasmic Sperm Injection. IEEE Trans Biomed Eng. 2018.Google Scholar

Lu, Z, Zhang, X, Leung, C, Esfandiari, N, Casper, RF, Sun, Y. Robotic ICSI (Intracytoplasmic Sperm Injection). IEEE Trans Biomed Eng 2011.Google Scholar

Han, C, Zhang, Q, Ma, R, Xie, L, Qiu, T, Wang, L, et al. Integration of Single Oocyte Trapping, in Vitro Fertilization and Embryo Culture in a Microwell-Structured Microfluidic Device. Lab Chip. 2010.Google Scholar

Kattera, S, Chen, C. Short Coincubation of Gametes in in Vitro Fertilization Improves Implantation and Pregnancy Rates: A Prospective, Randomized, Controlled Study. Fertil Steril. 2003; 80(4): 1017–21.Google Scholar

Bavister, BD. Culture of Preimplantation Embryos: Facts and Artifacts. Hum Reprod Update. 1995; 1(2): 91–148.Google Scholar

Gardner, DK, Lane, M. Culture of viable mammalian embryos. In: Cibelli, J, Lanza, R, Campbell, K, West, M (eds.), Principles of Cloning. San Diego: Academic Press. 2014; 63–84.CrossRef Google Scholar

Gardner, DK, Lane, M. Culture systems for the human embryo. In: Gardner, DK, Weissman, A, Howles, C, Shoham, Z (eds.) Textbook of Assisted Reproductive Techniques. Informa Healthcare. 2018; 200–24.Google Scholar

Pribenszky, C, Nilselid, A-M, Montag, M. Time-Lapse Culture with Morphokinetic Embryo Selection Improves Pregnancy and Live Birth Chances and Reduces Early Pregnancy Loss: A Meta-Analysis. Reprod Biomed Online. 2017; 35(5): 511–20.CrossRef Google Scholar PubMed

Paulson, RJ, Reichman, DE, Zaninovic, N, Goodman, LR, Racowsky, C. Time-Lapse Imaging: Clearly Useful to Both Laboratory Personnel and Patient Outcomes Versus Just Because We Can Doesn’t Mean We Should. Fertil Steril. 2018; 109(4): 584–91.Google Scholar

Armstrong, S, Bhide, P, Jordan, V, Pacey, A, Farquhar, C. Time-Lapse Systems for Embryo Incubation and Assessment in Assisted Reproduction. Cochrane database Syst Rev. 2018; 5:CD011320.Google Scholar

Wale, PL, Gardner, DK. The Effects of Chemical and Physical Factors on Mammalian Embryo Culture and Their Importance for the Practice of Assisted Human Reproduction. Hum Reprod Update. 2016; 22(1): 2–22.CrossRef Google Scholar PubMed

Gardner, DK, Kelley, RL. Impact of the IVF Laboratory Environment on Human Preimplantation Embryo Phenotype. J Dev Orig Health Dis. 2017; 8(4): 418–35.CrossRef Google Scholar PubMed

Bormann, C, Cabrera, L, Heo, YS, Takayama, S, Smith, G. Dynamic Microfluidic Embryo Culture Enhances Blastocyst Development of Murine and Bovine Embryos. Biol Reprod. 2017.Google Scholar

Heo, YS, Cabrera, LM, Bormann, CL, Shah, CT, Takayama, S, Smith, GD. Dynamic Microfunnel Culture Enhances Mouse Embryo Development and Pregnancy Rates. Hum Reprod. 2010.CrossRef Google Scholar

Gardner, DK, Pool, TB, Lane, M. Embryo Nutrition and Energy Metabolism and Its Relationship to Embryo Growth, Differentiation, and Viability. Semin Reprod Med. 2000.Google Scholar

Gardner, DK. Mammalian Embryo Culture in the Absence of Serum or Somatic Cell Support. Cell Biol Int.; 18(12): 1163–79.Google Scholar

Gardner, DK, Sakkas, D. Mouse Embryo Cleavage, Metabolism and Viability: Role of Medium Composition. Hum Reprod. 1993; 8(2).Google Scholar

Steeves, TE, Gardner, DK. Temporal and Differential Effects of Amino Acids on Bovine Embryo Development in Culture. Biol Reprod. 1999; 61(3): 731–40.Google Scholar

Lane, M, Gardner, DK. Differential Regulation of Mouse Embryo Development and Viability by Amino Acids. J Reprod Fertil. 1997; 109(1): 153–64.Google Scholar PubMed

Thouas, GA, Dominguez, F, Green, MP, Vilella, F, Simon, C, Gardner, DK. Soluble Ligands and Their Receptors in Human Embryo Development and Implantation. Endocrine Rev. 2015; 36(1): 92–130.CrossRef Google Scholar PubMed

Ferraz, MAMM, Rho, HS, Hemerich, D, Henning, HHW, van Tol HTA, Hölker, M, et al. An Oviduct-on-a-Chip Provides an Enhanced in Vitro Environment for Zygote Genome Reprogramming. Nat Commun. 2018.Google Scholar

Urbanski, JP, Johnson, MT, Craig, DD, Potter, DL, Gardner, DK, Thorsen, T. Noninvasive Metabolic Profiling Using Microfluidics for Analysis of Single Preimplantation Embryos. Anal Chem. 2008; 80(17): 6500–7.Google Scholar

Trounson, A, Mohr, L. Human Pregnancy Following Cryopreservation, Thawing and Transfer of an Eight-Cell Embryo. Nature. 1983.Google Scholar

Lane, M, Schoolcraft, WB, Gardner, DK. Vitrification of Mouse and Human Blastocysts Using a Novel Cryoloop Container-less Technique. Fertil Steril. 1999; 72(6): 1073–8.Google Scholar

Rienzi, L, Gracia, C, Maggiulli, R, LaBarbera, AR, Kaser, DJ, Ubaldi, FM, et al. Oocyte, Embryo and Blastocyst Cryopreservation in ART: Systematic Review and Meta-Analysis Comparing Slow-Freezing Versus Vitrification to Produce Evidence for the Development of Global Guidance. Hum Reprod Update. 2016.CrossRef Google Scholar

von Versen-Höynck, F, Schaub, AM, Chi, Y-Y, Chiu, K-H, Liu, J, Lingis, M, et al. Increased Preeclampsia Risk and Reduced Aortic Compliance With in Vitro Fertilization Cycles in the Absence of a Corpus Luteum. Hypertens. 1979; 73(3): 640–9.Google Scholar

Roy, TK, Brandi, S, Peura, TT. Chapter 20 Gavi-Automated Vitrification Instrument. Methods Mol Biol. 2017; 1568: 261–77.CrossRef Google Scholar PubMed

Arav, A, Natan, Y, Kalo, D, Komsky-Elbaz, A, Roth, Z, Levi-Setti, PE, et al. A New, Simple, Automatic Vitrification Device: Preliminary Results with Murine and Bovine Oocytes and Embryos. J Assist Reprod Genet. 2018; 35(7): 1161–8.Google Scholar

Pyne, DG, Liu, J, Abdelgawad, M, Sun, Y. Digital Microfluidic Processing of Mammalian Embryos for Vitrification. PLoS One. 2014; 9(9): e108128.Google Scholar

Gardner, DK, Balaban, B. Assessment of Human Embryo Development Using Morphological Criteria in an Era of Time-Lapse, Algorithms and “OMICS”: Is Looking Good Still Important? Mol Hum Reprod. 2016; 22(10): 704–18.Google Scholar

Gardner, DK, Meseguer, M, Rubio, C, Treff, NR. Diagnosis of Human Preimplantation Embryo Viability. Hum Reprod Update. 2015; 21(6): 727–47.CrossRef Google Scholar PubMed

Sanchez, T, Seidler, EA, Gardner, DK, Needleman, D, Sakkas, D. Will Noninvasive Methods Surpass Invasive for Assessing Gametes and Embryos? Fertil Steril. 2017; 108(5): 730–7.CrossRef Google Scholar PubMed

Gardner, DK, Reineck, P, Gibson, BC, Thompson, J. Microfluidics and Microanalytics to Facilitate Quantitative Assessment of Human Embryo Physiology. In: Agarwal, A, Varghese, A, Nagy, ZP (eds.) Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices. New Jersey: Humana Press. 2019.Google Scholar

Sanchez, T, Wang, T, Pedro, MV, Zhang, M, Esencan, E, Sakkas, D, et al. Metabolic Imaging with the Use of Fluorescence Lifetime Imaging Microscopy (FLIM) Accurately Detects Mitochondrial Dysfunction in Mouse Oocytes. Fertil Steril. 2018; 110(7): 1387–97.Google Scholar

Capalbo, A, Romanelli, V, Patassini, C, Poli, M, Girardi, L, Giancani, A, et al. Diagnostic Efficacy of Blastocoel Fluid and Spent Media as Sources of DNA for Preimplantation Genetic Testing in Standard Clinical Conditions. Fertil Steril. 2018; 110(5): 870–9.e5Google Scholar

Xu, J, Fang, R, Chen L, Chen D, Xiao J-P, Yang W, et al. Noninvasive Chromosome Screening of Human Embryos by Genome Sequencing of Embryo Culture Medium for in Vitro Fertilization. Proc Natl Acad Sci USA. 2016; 113(42): 11907–12.Google Scholar

Sutton-McDowall, ML, Gosnell, M, Anwer, AG, White, M, Purdey, M, Abell, AD, et al. Hyperspectral Microscopy Can Detect Metabolic Heterogeneity Within Bovine Post-Compaction Embryos Incubated under Two Oxygen Concentrations (7% Versus 20%). Hum Reprod. 2017; 32(10): 2016–25.Google Scholar

Liu, Y, Chapple, V, Feenan, K, Roberts, P, Matson, P. Time-Lapse Deselection Model for Human Day 3 in Vitro Fertilization Embryos: The Combination of Qualitative and Quantitative Measures of Embryo Growth. Fertil Steril. 2016; 105(3): 656–62.e1.Google Scholar

Petersen, BM, Boel, M, Montag, M, Gardner, DK. Development of a Generally Applicable Morphokinetic Algorithm Capable of Predicting the Implantation Potential of Embryos Transferred on Day 3. Hum Reprod. 2016; 31(10).Google Scholar

Khosravi, P, Kazemi, E, Zhan, Q, Malmsten, JE, Toschi, M, Zisimopoulos, P, et al. Deep Learning Enables Robust Assessment and Selection of Human Blastocysts After in Vitro Fertilization. Nat Digit Med. 2019; 2.Google Scholar

Tran, A, Cooke, S, Illingworth, PJ, Gardner, DK. A Study of the Diagnostic Accuracy of Deep Learning, a Novel Approach for Predicting the Fetal Heart Implantation Rate Following Time-Lapse Incubation. Hum Reprod. 2019; in press.Google Scholar

Sakkas, D, Brent Barrett, C, Alper, MM. Types and Frequency of Non-Conformances in an IVF Laboratory. Hum Reprod. 2018; 33(12).Google Scholar

Nagamatsu, G, Hayashi, K. Stem Cells, in Vitro Gametogenesis and Male Fertility. Reproduction. 2017; 154(6): F79–91.Google Scholar

Ma, H, Marti-Gutierrez, N, Park, S-W, Wu, J, Lee, Y, Suzuki, K, et al. Correction of a Pathogenic Gene Mutation in Human Embryos. Nature. 2017; 548(7668): 413–19.Google Scholar

Hayashi, K, Ogushi, S, Kurimoto, K, Shimamoto, S, Ohta, H, Saitou, M. Offspring from Oocytes Derived from in Vitro Primordial Germ Cell-like Cells in Mice. Science (80-). 2012; 338(6109): 971–5.Google Scholar

Hayashi, K, Saitou, M. Generation of Eggs from Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells. Nat Protoc. 2013; 8(8): 1513–24.Google Scholar

Richani, D, Constance, K, Lien, S, Agapiou, D, Stocker, WA, Hedger, MP, et al. Cumulin and FSH Cooperate to Regulate Inhibin B and Activin B Production by Human Granulosa-Lutein Cells In Vitro. Endocrinology. 2019; 160(4): 853–62.CrossRef Google Scholar PubMed

Vuong, LN, Ho, VNA, Ho, TM, Dang, VQ, Phung, TH, Giang, NH, et al. Effectiveness and Safety of in Vitro Maturation of Oocytes Versus in Vitro Fertilisation in Women with High Antral Follicle Count: Study Protocol for a Randomised Controlled Trial. 2018; 8(12): e023413.Google Scholar

Treff, NR, Zimmerman, R, Bechor, E, Hsu, J, Rana, B, Jensen, J, et al. Validation of Concurrent Preimplantation Genetic Testing for Polygenic and Monogenic Disorders, Structural Rearrangements, and Whole and Segmental Chromosome Aneuploidy with a Single Universal Platform. Eur J Med Genet. 2019.CrossRef Google Scholar