Book contents
- Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
- Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
- Copyright page
- Contents
- Contributors
- Foreword
- Chapter 1 In Vitro Fertilization and Micromanipulation
- Chapter 2 Development of ICSI in Human Assisted Reproduction
- Chapter 3 Current ICSI Applications and Clinical Outcomes
- Chapter 4 Rescue ICSI of IVF Failed-Fertilized Oocytes
- Chapter 5 Morphological Sperm Selection Before ICSI
- Chapter 6 Laser-Assisted ICSI
- Chapter 7 Piezo: The Add-On to Standardize ICSI Procedure
- Chapter 8 Artificial Oocyte Activation After ICSI
- Chapter 9 Health of Children Born after Intracytoplasmic Sperm Injections (ICSI)
- Chapter 10 Examining the Safety of ICSI Using Animal Models
- Chapter 11 Cellular and Molecular Events after ICSI in Clinically Relevant Animal Models
- Chapter 12 Micromanipulation, Micro-Injection Microscopes and Systems for ICSI
- Chapter 13 Automation Techniques and Systems for ICSI
- Chapter 14 Germline Nuclear Transfer Technology to Overcome Mitochondrial Diseases and Female Infertility
- Chapter 15 Nuclear Transfer Technology and Its Use in Reproductive Medicine
- Chapter 16 The Prospects of Infertility Treatment Using “Artificial” Eggs
- Index
- Plate Section (PDF Only)
- References
Chapter 11 - Cellular and Molecular Events after ICSI in Clinically Relevant Animal Models
Published online by Cambridge University Press: 02 December 2021
- Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
- Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
- Copyright page
- Contents
- Contributors
- Foreword
- Chapter 1 In Vitro Fertilization and Micromanipulation
- Chapter 2 Development of ICSI in Human Assisted Reproduction
- Chapter 3 Current ICSI Applications and Clinical Outcomes
- Chapter 4 Rescue ICSI of IVF Failed-Fertilized Oocytes
- Chapter 5 Morphological Sperm Selection Before ICSI
- Chapter 6 Laser-Assisted ICSI
- Chapter 7 Piezo: The Add-On to Standardize ICSI Procedure
- Chapter 8 Artificial Oocyte Activation After ICSI
- Chapter 9 Health of Children Born after Intracytoplasmic Sperm Injections (ICSI)
- Chapter 10 Examining the Safety of ICSI Using Animal Models
- Chapter 11 Cellular and Molecular Events after ICSI in Clinically Relevant Animal Models
- Chapter 12 Micromanipulation, Micro-Injection Microscopes and Systems for ICSI
- Chapter 13 Automation Techniques and Systems for ICSI
- Chapter 14 Germline Nuclear Transfer Technology to Overcome Mitochondrial Diseases and Female Infertility
- Chapter 15 Nuclear Transfer Technology and Its Use in Reproductive Medicine
- Chapter 16 The Prospects of Infertility Treatment Using “Artificial” Eggs
- Index
- Plate Section (PDF Only)
- References
Summary
Interactions between the sperm-borne oocyte activating factor(s) (SOAF) and the molecules within the oocyte cytoplasm, trigger the molecular pathways associated with oocyte activation. Of note, release of calcium from the endoplasmic reticulum (ER) creates calcium oscillations in mammals and one large calcium release in non-mammalian species. As the newly activated ovum begins to transform, the components of the fertilizing spermatozoon function cooperatively with the contents of the ooplasm as development progresses. The spermatozoon has been shown to contribute the main microtubule framework to the embryo through the integration of its centriole and has also been implicated in facilitating processes. Through oocyte-driven mechanisms, unnecessary sperm components must also be degraded. Many of these processes are centered around the reducing power of oocyte-produced glutathione (GSH) and facilitated by both the sperm-borne and oocyte-borne enzymatic activity. In this chapter we explore underlying molecular and biochemical processes that drive developmental progression of newly fertilized oocytes, and how ICSI procedures could change the molecular pathways found in natural fertilization.
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- Information
- Manual of Intracytoplasmic Sperm Injection in Human Assisted ReproductionWith Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte, pp. 103 - 113Publisher: Cambridge University PressPrint publication year: 2021