Book contents
- Male and Sperm Factors that Maximize IVF Success
- Male and Sperm Factors that Maximize IVF Success
- Copyright page
- Contents
- Contributors
- Chapter 1 Sperm Selection for ART Success
- Chapter 2 New Horizons in Male Subfertility and Infertility
- Chapter 3 Chromosome Abnormalities and the Infertile Male
- Chapter 4 The Effect of Endocrine Disruptors and Environmental and Lifestyle Factors on the Sperm Epigenome
- Chapter 5 Lifestyle Factors and Sperm Quality
- Chapter 6 The Effect of Age on Male Fertility and the Health of Offspring
- Chapter 7 The Assessment and Role of Anti-sperm Antibodies
- Chapter 8 FSH Treatment in Male Infertility
- Chapter 9 Antioxidants to Improve Sperm Quality
- Chapter 10 The History of Utilization of IVF for Male Factor Subfertility
- Chapter 11 The Case Against Intracytoplasmic Sperm Injection for All
- Chapter 12 Perinatal Outcomes from IVF and ICSI
- Chapter 13 Artificial Insemination with Partner’s Sperm for Male Subfertility
- Chapter 14 Obstructive Azoospermia: Is There a Place for Microsurgical Testicular Sperm Extraction?
- Chapter 15 Should Varicocele Be Operated on Before IVF?
- Chapter 16 Donor Insemination: Past, Present and Future Perspectives
- Chapter 17 DNA Damage in Spermatozoa
- Chapter 18 Prevention of Male Infertility: From Childhood to Adulthood
- Index
- References
Chapter 1 - Sperm Selection for ART Success
Published online by Cambridge University Press: 24 May 2020
- Male and Sperm Factors that Maximize IVF Success
- Male and Sperm Factors that Maximize IVF Success
- Copyright page
- Contents
- Contributors
- Chapter 1 Sperm Selection for ART Success
- Chapter 2 New Horizons in Male Subfertility and Infertility
- Chapter 3 Chromosome Abnormalities and the Infertile Male
- Chapter 4 The Effect of Endocrine Disruptors and Environmental and Lifestyle Factors on the Sperm Epigenome
- Chapter 5 Lifestyle Factors and Sperm Quality
- Chapter 6 The Effect of Age on Male Fertility and the Health of Offspring
- Chapter 7 The Assessment and Role of Anti-sperm Antibodies
- Chapter 8 FSH Treatment in Male Infertility
- Chapter 9 Antioxidants to Improve Sperm Quality
- Chapter 10 The History of Utilization of IVF for Male Factor Subfertility
- Chapter 11 The Case Against Intracytoplasmic Sperm Injection for All
- Chapter 12 Perinatal Outcomes from IVF and ICSI
- Chapter 13 Artificial Insemination with Partner’s Sperm for Male Subfertility
- Chapter 14 Obstructive Azoospermia: Is There a Place for Microsurgical Testicular Sperm Extraction?
- Chapter 15 Should Varicocele Be Operated on Before IVF?
- Chapter 16 Donor Insemination: Past, Present and Future Perspectives
- Chapter 17 DNA Damage in Spermatozoa
- Chapter 18 Prevention of Male Infertility: From Childhood to Adulthood
- Index
- References
Summary
This chapter describes techniques for the preparation of human spermatozoa for assisted conception. Traditional swim-up techniques depend on the intrinsic motility of human spermatozoa and have been used as the basis for new microfluidics devices that exploit the capacity of these cells for chemotaxis, rheotaxis or thermotaxis. Unfortunately, such self-migration strategies tend to lose effectiveness with pathological samples exhibiting poor levels of motility and/or diminished counts. With such compromised starting material, active selection of spermatozoa can be achieved by exploiting differences between sperm populations in their density or charge. Density gradient centrifugation selects spermatozoa with minimal residual cytoplasm that are highly motile and have good morphology, even though the induction of sperm DNA damage in certain patients may be problematical. Alternatively, methodologies based on differences in sperm charge, appear to hold promise for the rapid, efficient isolation of highly motile human spermatozoa that consistently exhibit low levels of DNA damage.
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- Male and Sperm Factors that Maximize IVF Success , pp. 1 - 14Publisher: Cambridge University PressPrint publication year: 2020