This chapter shows how human obedience is captured in an experimental setup, and how such research methodology can help us understand how people can comply with orders to hurt another person on a neurological level. By reviewing past experimental research, such as the rat decapitation study of Landis, the studies of Stanley Milgram on destructive obedience, and the Utrecht studies on obedience to non-ethical requests, this chapter shows that under certain circumstances, a majority of individuals could be coerced into inflicting physical or psychological harm on others at levels generally deemed unacceptable, even without any tangible social pressures such as military court or job loss. The chapter also describes a novel method where people can administer real painful electric shocks to someone else in exchange for a small monetary reward, and describes how such a method allows neuroscience investigations that would focus on the neural mechanisms associated with obedience.