The appearance of sequence stratigraphy in the late eighties resulted in a revolution in the study of sedimentary rocks. The shift from seismic stratigraphy (Vail et al., 1977) to sequence stratigraphy brought the incorporation of outcrops and cores as sources of data in stratigraphic analysis (Posamentier et al., 1988; Posamentier and Vail, 1988; Van Wagoner et al., 1990). Coincident with this shift, ichnological studies began to emphasize the importance of trace fossils in sequence stratigraphy (e.g. Savrda, 1991b; MacEachern et al., 1992; Pemberton et al., 1992b). In little more than a decade, the field experienced a rapid increase in the number of studies devoted to exploring the applicability of ichnology in refining sequence-stratigraphic analysis (e.g. MacEachern et al., 1992, 1999a, 2007c; Savrda et al., 1993; Taylor and Gawthorpe, 1993; Pemberton and MacEachern, 1995; Ghibaudo et al., 1996; Martin and Pollard, 1996; Buatois et al., 1998d, 2002b; Pemberton et al., 2001, 2004; Carmona et al., 2006). At present, ichnological aspects are currently covered in sequence-stratigraphic textbooks (e.g. Catuneanu, 2006). The aim of this chapter is to provide a detailed review of the applications of ichnology in sequence stratigraphy. Although a large part of this chapter deals with the recognition of discontinuity surfaces in marine siliciclastic successions, we will also cover other topics which are commonly overlooked in the literature. These include characterization of parasequences, parasequence sets, and systems tracts, but also the potential of trace fossils to address sequence-stratigraphic issues in carbonates and continental deposits.