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20 - Role and efficiency of artificial insemination and genome resource banking

Published online by Cambridge University Press:  09 August 2009

Jogayle Howard
Affiliation:
National Zoological Park
Yan Huang
Affiliation:
China Conservation and Research Center for the Giant Panda
Pengyan Wang
Affiliation:
China Research and Conservation Center for the Giant Panda
Desheng Li
Affiliation:
China Conservation and Research Center for the Giant Panda
Guiquan Zhang
Affiliation:
China Research and Conservation Center for the Giant Panda
Rong Hou
Affiliation:
Chengdu Research Base of Giant Panda Breeding
Zhihe Zhang
Affiliation:
Chengdu Research Base of Giant Panda Breeding
Barbara S. Durrant
Affiliation:
Conservation and Research for Endangered Species
Rebecca Spindler
Affiliation:
Toronto Zoo
Hemin Zhang
Affiliation:
China Conservation and Research Center for the Giant Panda
Anju Zhang
Affiliation:
Chengdu Giant Panda Breeding Research Foundation
David E. Wildt
Affiliation:
National Zoological Park
David E. Wildt
Affiliation:
Smithsonian National Zoological Park, Washington DC
Anju Zhang
Affiliation:
Chengdu Research Base of Giant Panda Breeding
Hemin Zhang
Affiliation:
Wildlife Conservation and Research Center for Giant Pandas
Donald L. Janssen
Affiliation:
Zoological Society of San Diego
Susie Ellis
Affiliation:
Conservation Breeding Specialist Group
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Summary

INTRODUCTION

Historically, the breeding of giant pandas in ex situ programmes has been difficult due to behavioural incompatibility and interanimal aggression. Because some individuals fail to mate naturally, the potential loss of valuable genes is a major concern to effective genetic management (see Chapter 21). Consistently successful artificial insemination (AI) would allow incorporating genetically valuable males with behavioural or physical anomalies into the gene pool. This strategy becomes even more powerful when used in the context of a genome resource bank (GRB), an organised repository of cryopreserved biomaterials (tissue, blood, DNA and sperm) (see Chapter 7). The use of sperm cryopreservation and AI allows the movement of genes among zoos and breeding centres without needing to transfer animals, which is both stressful and costly.

‘Assisted breeding’ refers to the tools and techniques associated with helping a pair of animals propagate, from AI to embryo transfer to cloning, among others (Howard, 1999; Pukazhenthi & Wildt, 2004). With the exception of AI, there is not much need for most other assisted-breeding techniques for the giant panda. As will be demonstrated here, AI is quite adequate for dealing with most cases of infertility or with helping to maintain adequate gene diversity in the captive population. In fact, the major breeding facilities, especially the China Conservation and Research Centre for the Giant Panda (hereafter referred to as the Wolong Breeding Centre) and the Chengdu Research Base of Giant Panda Breeding, routinely use AI to increase pregnancy success.

Type
Chapter
Information
Giant Pandas
Biology, Veterinary Medicine and Management
, pp. 469 - 494
Publisher: Cambridge University Press
Print publication year: 2006

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References

Bonney, R. C., Wood, D. J. and Kleiman, D. G. (1982). Endocrine correlates of behavioural oestrus in the female giant panda (Ailuropoda melanoleuca) and associated hormonal changes in the male. Journal of Reproduction and Fertility, 64, 209–15.CrossRefGoogle Scholar
Durrant, B., Czekala, N., Olson, M.et al. (2002). Papanicolaou staining of exfoliated vaginal epithelial cells facilitates the prediction of ovulation in the giant panda. Theriogenology, 57, 1855–64.CrossRefGoogle ScholarPubMed
Durrant, B. S., Olson, M. A., Amodeo, D.et al. (2003). Vaginal cytology and vulvar swelling as indicators of impending estrus and ovulation in the giant panda (Ailuropoda melanoleuca). Zoo Biology, 22, 313–21.CrossRefGoogle Scholar
Hodges, J. K., Bevan, D. J., Celma, M.et al. (1984). Aspects of reproductive endocrinology of the female giant panda (Ailuropoda melanoleuca) in captivity with special reference to the detection of ovulation and pregnancy. Journal of Zoology (London), 203, 253–67.CrossRefGoogle Scholar
Howard, J. G. (1999). Assisted reproductive techniques in nondomestic carnivores. In Zoo and Wild Animal Medicine. Current Therapy IV, ed. Fowler, M. E. and Miller, R. E.. Philadelphia, PA: W. B. Saunders Co., pp. 449–57.Google Scholar
Hu, J. and Wei, F. (1990). Development and progress of breeding and rearing giant pandas in captivity within China. In Research and Progress in Biology of the Giant Panda, ed. Hu, J.. Chengdu: Sichuan Publishing House of Science and Technology, pp. 322–5.Google Scholar
Hu, J., Liu, T. and He, G. (1990). Giant Pandas with Graceful Bearing. Chengdu: Sichuan Publishing House of Science and Technology.Google Scholar
Huang, Y., Li, D. S., Du, J.et al. (2000a). Cryopreservation experiment on sperm of the giant panda. Chinese Journal of Veterinary Medicine, 26, 13–14.Google Scholar
Huang, Y., Li, D. S., Zhang, H. M.et al. (2000b). Electroejaculation and semen cryopreservation in the giant panda. Journal of Sichuan Teachers College (Natural Science), 21, 238–43.Google Scholar
Huang, Y., Wang, P. Y., Zhang, H. M.et al. (2001). Efficiency of artificial insemination in giant pandas at the Wolong Breeding Center. Journal of Andrology Supplement, abstract 118.Google Scholar
Huang, Y., Wang, P., Zhang, G. et al. (2002). Use of artificial insemination to enhance propagation of giant pandas at the Wolong Breeding Center. In Proceeding of the Second International Symposium on Assisted Reproductive Technology for the Conservation and Genetic Management of Wildlife, ed. Loskutoff, N.. Omaha, NE: Omaha's Henry Doorly Zoo, pp. 172–9.Google Scholar
Kan, O. and Shu-hua, T. (1964). In the Peking Zoo – the first baby giant panda. Animal Kingdom, 57, 44–6.Google Scholar
Kleiman, D. G., Karesh, W. B. and Chu, P. R. (1979). Behavioural changes associated with oestrus in the giant panda (Ailuropoda melanoleuca) with comments on female proceptive behaviour. International Zoo Yearbook, 19, 217–23.CrossRefGoogle Scholar
Liu, W. (1979). The test of giant panda artificial insemination. Chinese Science Report, 9, 415.Google Scholar
Liu, W. (1981). A note on the artificial insemination of the giant panda. Acta Veterinaria et Zootechnica Sinica, 12, 73–6.Google Scholar
Liu, W. X., Ye, J. Q., Li, C. Z. and Liao, G. X. (1979). Artificial insemination experiment on the giant panda. The Chinese Zoo Annual, 2, 20–4.Google Scholar
Masui, M., Hiramatsu, H., Nose, N.et al. (1989). Successful artificial insemination in the giant panda (Ailuropoda melanoleuca) at Ueno Zoo. Zoo Biology, 8, 17–26.CrossRefGoogle Scholar
Moore, H. D. M., Bush, M., Celma, M.et al. (1984). Artificial insemination in the giant panda (Ailuropoda melanoleuca). Journal of Zoology (London), 203, 269–78.CrossRefGoogle Scholar
Olson, M. A., Huang, Y., Li, D.et al. (2003a). Assessment of motility, acrosomal integrity and viability of giant panda (Ailuropoda melanoleuca) sperm following short-term storage at 4°C. Zoo Biology, 22, 529–44.CrossRefGoogle Scholar
Olson, M. A., Huang, Y., Li, D., Zhang, H. and Durrant, B. (2003b). Comparison of storage techniques for giant panda sperm. Zoo Biology, 22, 335–45.CrossRefGoogle Scholar
Pukazhenthi, B. S. and Wildt, D. E. (2004). Which reproductive technologies are most relevant to studying, managing and conserving wildlife?Reproduction, Fertility and Development, 16, 33–46.CrossRefGoogle ScholarPubMed
Wildt, D. E. (1997). Genome resource banking: impact on biotic conservation and society. In Reproductive Tissue Banking: Scientific Principles, ed. Karow, A. M. and Critser, J. K.. San Diego, CA: Academic Press, pp. 399–439.Google Scholar
Wildt, D. E., Rall, W. F., Critser, J. K., Monfort, S. L. and Seal, U. S. (1997). Genome resource banks: ‘living collections’ for biodiversity conservation. BioScience, 47, 689–98.CrossRefGoogle Scholar
Ye, Z. Y., He, G. X., Zhang, A. J.et al. (1991). Studies on the artificial pollination method of the giant panda. Journal of Sichuan University (Natural Science), 28, 50–3.Google Scholar
Zeng, G. Q., Meng, Z. B., Jiang, G. T., He, G. X. and Xu, Q. M. (1984). The relationship between estrogen concentration and estrous behaviors during the estrous cycle of the giant panda. Acta Veterinaria et Zootechnica Sinica, 30, 324–30.Google Scholar
Zhang, A. J., Ye, Z. Y., He, G. X.et al. (1991). Studies on the conception effect of frozen semen in the giant panda. Journal of Sichuan University (Natural Science), 28, 54–9.Google Scholar

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