Book contents
- Frontmatter
- Contents
- List of contributors
- Preface
- 1 Self-integration – an emerging concept from the fungal mycelium
- 2 Nutrient translocation and electrical signalling in mycelia
- 3 Colony development in nutritionally heterogeneous enviromnents
- 4 Circadian rhythms in filamentous fungi
- 5 Growth, branching and enzyme production by filamentous fungi in submerged culture
- 6 Metabolism and hyphal differentiation in large basidiomycete colonies
- 7 Role of phosphoinositides and inositol phosphates in the regulation of mycelial branching
- 8 Stress responses of fungal colonies towards toxic metals
- 9 Cellularization in Aspergillus nidulans
- 10 Genetic control of polarized growth and branching in filamentous fungi
- 11 Mating and sexual interactions in fungal mycelia
- 12 Genetic stability in fungal mycelia
- 13 Nuclear distribution and gene expression in the secondary mycelium of Schizophyllum commune
- Index
5 - Growth, branching and enzyme production by filamentous fungi in submerged culture
Published online by Cambridge University Press: 22 January 2010
- Frontmatter
- Contents
- List of contributors
- Preface
- 1 Self-integration – an emerging concept from the fungal mycelium
- 2 Nutrient translocation and electrical signalling in mycelia
- 3 Colony development in nutritionally heterogeneous enviromnents
- 4 Circadian rhythms in filamentous fungi
- 5 Growth, branching and enzyme production by filamentous fungi in submerged culture
- 6 Metabolism and hyphal differentiation in large basidiomycete colonies
- 7 Role of phosphoinositides and inositol phosphates in the regulation of mycelial branching
- 8 Stress responses of fungal colonies towards toxic metals
- 9 Cellularization in Aspergillus nidulans
- 10 Genetic control of polarized growth and branching in filamentous fungi
- 11 Mating and sexual interactions in fungal mycelia
- 12 Genetic stability in fungal mycelia
- 13 Nuclear distribution and gene expression in the secondary mycelium of Schizophyllum commune
- Index
Summary
Introduction
This chapter considers (a) the generation of highly branched (colonial)mutants during prolonged fermentation of the Quorn® myco-protein fungus, Fusarium graminearum A3/5, (b) the influence of hyphal branch frequency on the production of extracellular enzymes by Aspergillus oryzae, and (c) the stability of Aspergillus niger gla A transformants in prolonged continuous how cultures. We believe that the results obtained with these three species can be extended to most, if not all, filamentous fungi.
Highly branched mutants do not have a selective advantage because of their morphology
When filamentous fungi or streptomycetes are grown in prolonged, continuous culture, it is common for the relatively sparsely branched parental strain to be supplanted by a relatively highly branched mutant (Fig. 5.1). Such mutants are called colonial because in Petri dish culture they form dense colonies that expand in radius more slowly than parentalcolonies. Selection of colonial mutants has been observed in continuouscultures of Byssochlamys nivea, Paecilomyces variotii, Paecilomyces puntanii, Gliocladium virens, Trichoderma viride (Forss et al., 1974), Penicillium chrysogerrum (Righelato, 1976), F. graminearum (Solomons & Scammell, 1976) and Acremonium chrysogemrm (A. Trilli, personal communication).
- Type
- Chapter
- Information
- The Fungal Colony , pp. 108 - 125Publisher: Cambridge University PressPrint publication year: 1999
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