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15 - Genetical Genomics Data: Some Statistical Problems and Solutions

Published online by Cambridge University Press:  05 June 2013

Hongzhe Li
Affiliation:
University of Pennsylvania
Kim-Anh Do
Affiliation:
University of Texas, MD Anderson Cancer Center
Zhaohui Steve Qin
Affiliation:
Emory University, Atlanta
Marina Vannucci
Affiliation:
Rice University, Houston
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Summary

Introduction and Review of Current Methods

DNA variants have been shown to alter the expression levels and patterns of many human genes in different tissues. Genetic loci responsible for such genetic controls of gene expression are known as expression quantitative trait loci (eQTLs). Such genetical genomics data of genetically diverse individuals provide rich information on gene regulation and links to complex phenotypes. Most commonly used methods for eQTL data analysis focus on identifying genetic variants that affect the mean expression levels of genes. However, effects of genetic variation on gene expression regulation can also be at the co-expression level. We first review some standard methods for eQTL data analysis, focusing on methods for identifying cis- and trans-eQTLs.

Expression Quantitative Trait Loci (eQTL)

Technology advances in large-scale genotyping and genome-wide gene expression measurements are increasingly able to investigate the role of genetic and regulatory variation in different biological contexts (Montgomery and Dermitzakis, 2011). Recent research has focused on understanding how DNA sequence variation can explain variation of gene expression in different tissues and different cellular states (Cheung and Spielman, 2002; Brem and Kruglyak, 2005). Genetics of gene expression studies involve identification of genetic loci that are associated with gene expression variations (eQTLs). Such studies have provided important information on the location and impact of regulatory variants, the tissue specificity of regulatory elements, and the contribution of cis- versus trans-acting variation to the expression of any given gene (Gaffney et al., 2012).

Type
Chapter
Information
Advances in Statistical Bioinformatics
Models and Integrative Inference for High-Throughput Data
, pp. 312 - 330
Publisher: Cambridge University Press
Print publication year: 2013

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