Three herbicide premixes have recently been introduced for weed control in wheat: halauxifen + florasulam, thifensulfuron + fluroxypyr, and bromoxynil + bicyclopyrone. The objective of this study was to evaluate these herbicides along with older products for their control of small-seeded false flax in winter wheat in Oklahoma. Studies took place during the 2017, 2018, and 2020 winter wheat growing seasons. Weed control was visually estimated every 2 wk throughout the growing season, and wheat yield was collected in all 3 yr. Small-seeded false flax diameter was approximately 6 cm at the time of application in all years. Control ranged from 96% to 99% following all treatments with the exception of bicyclopyrone + bromoxynil and dicamba alone, which controlled false flax 90%. All treatments containing an acetolactate synthase (ALS)–inhibiting herbicide achieved adequate control; therefore, resistance is not suspected in this population. Halauxifen + florasulam and thifensulfuron + fluroxypyr effectively controlled small-seeded false flax similarly to other standards recommended for broadleaf weed control in wheat in Oklahoma. Rotational use of these products allows producers flexibility in controlling small-seeded false flax and reduces the potential for development of herbicide resistance in this species.

Horseweed is a competitive summer or winter annual weed that produces up to 230,000 small seeds per plant that are capable of traveling more than 500 km via wind. Giant ragweed is a tall, highly competitive summer annual weed. Glyphosate-resistant (GR) horseweed and GR giant ragweed pose significant challenges for producers in the United States and Ontario, Canada. It is thought that an integrated weed management (IWM) system involving herbicide rotation is required to control GR biotypes. Halauxifen-methyl is a new selective broadleaf POST herbicide registered for use in cereal crops; there is limited information on its efficacy on horseweed and giant ragweed. The purpose of this research was to determine the efficacy of halauxifen-methyl applied POST, alone and in a tank mix, for the control of GR horseweed and GR giant ragweed in wheat across southwestern Ontario. For each weed species, an efficacy study consisting of six field experiments was conducted over a 2-yr period (2018, 2019). At 8 wk after application (WAA), halauxifen-methyl, fluroxypyr/halauxifen-methyl, fluroxypyr/halauxifen-methyl + MCPA EHE, fluroxypyr + MCPA ester, 2,4-D ester, clopyralid, and pyrasulfotole/bromoxynil + ammonium sulfate controlled GR horseweed >95%. Fluroxypyr and MCPA provided only 86% and 37% control of GR horseweed, respectively. At 8 WAA, fluroxypyr, fluroxypyr/halauxifen-methyl, fluroxypyr/halauxifen-methyl + MCPA EHE, fluroxypyr + MCPA ester, fluroxypyr/halauxifen-methyl + MCPA EHE + pyroxsulam, 2,4-D ester, clopyralid, and thifensulfuron/tribenuron + fluroxypyr + MCPA ester controlled GR giant ragweed 87%, 88%, 90%, 94%, 96%, 96%, 98%, and 93%, respectively. Halauxifen-methyl and pyroxsulam provided only 45% and 28% control of GR giant ragweed, respectively. Halauxifen-methyl applied alone POST in the spring controlled GR horseweed but not GR giant ragweed in winter wheat.

Halauxifen plus florasulam, thifensulfuron plus fluroxypyr, and bromoxynil plus bicyclopyrone are three, relatively new POST premix herbicides developed for control of broadleaf weeds in winter wheat. These herbicides, along with older products, were evaluated for their control of horseweed in Altus, Perkins, and Ponca City, Oklahoma, during the spring of 2017 and 2018. Horseweed has become a critical weed in Oklahoma because of its extensive germination window, changes in tillage practices, and increase in herbicide-resistant horseweed biotypes. Visual weed control was estimated every 2 wk throughout the growing season and wheat yield was collected from three of the six site-years. Horseweed size ranged from 5 to 20 cm at time of application. The halauxifen plus florasulam, and thifensulfuron plus fluroxypyr combinations were effective at controlling a wide range of horseweed rosette sizes across all locations, whereas control with other treatments varied depending on presence of herbicide resistance, weed size at time of application, and mix partner.

In recent years, the use of cover crops has increased in U.S. crop production systems. An important aspect of successful cover crop establishment is the preceding crop and herbicide program, because some herbicides have the potential to persist in the soil for several months. Few studies have been conducted to evaluate the sensitivity of cover crops to common residual herbicides used in soybean production. The same field experiment was conducted in 2016 in Arkansas, Illinois, Indiana, Missouri, Tennessee, and Wisconsin, and repeated in Arkansas, Illinois, Indiana, Mississippi, and Missouri in 2017 to evaluate the potential of residual soybean herbicides to carryover and reduce cover crop establishment. Herbicides applied during the soybean growing season included acetochlor; acetochlor plus fomesafen; chlorimuron plus thifensulfuron; fomesafen; fomesafen plus S-metolachlor followed by acetochlor; imazethapyr; pyroxasulfone; S-metolachlor; S-metolachlor plus fomesafen; sulfentrazone plus S-metolachlor; sulfentrazone plus S-metolachlor followed by fomesafen plus S-metolachlor; and sulfentrazone plus S-metolachlor followed by fomesafen plus S-metolachlor followed by acetochlor. Across all herbicide treatments, the sensitivity of cover crops to herbicide residues in the fall, from greatest to least, was forage radish = turnip > annual ryegrass = winter oat = triticale > cereal rye = Austrian winter pea = hairy vetch = wheat > crimson clover. Fomesafen (applied 21 and 42 days after planting [(DAP]); chlorimuron plus thifensulfuron and pyroxasulfone applied 42 DAP; sulfentrazone plus S-metolachlor followed by fomesafen plus S-metolachlor; and sulfentrazone plus S-metolachlor followed by fomesafen plus S-metolachlor followed by acetochlor caused the highest visual ground cover reduction to cover crop species at the fall rating. Study results indicate cover crops are most at risk when following herbicide applications in soybean containing certain active ingredients such as fomesafen, but overall there is a fairly low risk of cover crop injury from residual soybean herbicides applied in the previous soybean crop.

In 2015, winter wheat growers in Virginia reported commercial failures of thifensulfuron to control mouse-ear cress. This was the first reported case of field-evolved acetolactate synthase (ALS) resistance in mouse-ear cress, so research was conducted to evaluate alternative herbicide options as well as to document potential yield loss in winter wheat from mouse-ear cress. Efficacy studies were conducted at three site-years in 2015 to 2016 and 2016 to 2017 as well as a POST greenhouse trial. In the PRE study, flumioxazin, pyroxasulfone, saflufenacil, and metribuzin resulted in more than 80% mouse-ear cress control 15 wk after planting across all sites with no observable wheat injury. No differences were observed in wheat yield in two of three sites in the PRE herbicide study; yield differences were attributed to common chickweed and not to mouse-ear cress. In the POST herbicide study, 2,4-D, dicamba, and metribuzin resulted in greater than 75% control in the field and greenhouse. Metribuzin, dicamba, and pyroxsulam resulted in crop injury 3 wk after treatment at some sites, but injury was transient. Yield from all POST treatments was similar to the nontreated plots. No yield loss was observed by mouse-ear cress densities greater than 300 plants m–2, indicating that mouse-ear cress is not very competitive with winter wheat. Growers should make herbicide decisions based on other weeds in the field and can incorporate the aforementioned herbicides for mouse-ear cress control.

Palmer amaranth is the most economically damaging glyphosate-resistant (GR) weed in the southern United States. An understanding of the basic biology, including relative growth and competitiveness of GR and glyphosate-susceptible (GS) Palmer amaranth phenotypes from a segregating population collected from the same geographical location, may yield information helpful in the management of resistant populations. A segregating population of Palmer amaranth collected in North Carolina during 2010 was used as a plant source for both GR and GS traits. Research was conducted in the greenhouse to compare the following: level of resistance and shikimate accumulation in GR and GS phenotypes following glyphosate application; interference from GR and GS phenotypes on early-season vegetative growth of corn, cotton, and peanut; effect of various durations of imposed drought stress on GR and GS phenotypes; and response of GR and GS phenotypes to POST-applied herbicides. The GR50 (glyphosate rate providing 50% reduction in shoot dry biomass) was 17 times greater with the GR phenotype compared with the GS phenotype. Shikimate accumulated in both GR and GS phenotypes following glyphosate application, but greater concentrations were found in GS plants. The GR and GS phenotypes responded similarly when subjected to drought stress; grown with corn, cotton, and peanut; or treated with 2,4-D, atrazine, dicamba, fomesafen, glufosinate, paraquat, tembotrione, and thifensulfuron. These results indicate that in the absence of glyphosate selection pressure, resistance to glyphosate does not influence the growth and competitiveness of GR and GS Palmer amaranth phenotypes collected from the same geographical location.

Greenhouse and laboratory studies were conducted to determine the degree of dominance of the monogenic sulfonylurea herbicide resistance trait in diploid sugarbeet by comparing the response of homozygous and heterozygous resistant sugarbeet to primisulfuron, thifensulfuron, and chlorimuron on the whole plant and acetolactate synthase (ALS) enzyme level. Progeny tests suggested that the monogenic sulfonylurea herbicide resistance was semidominant. Subsequently, heterozygous resistant (R-1) and homozygous resistant (R-2) sugarbeet lines were sprayed with increasing rates of primisulfuron, thifensulfuron, and chlorimuron, and herbicide rates required for 50% growth reduction (GR50) were determined. GR50 values were also determined for homozygous susceptible sugarbeet lines (S-1 and S-2). The GR50 values indicated that the R-2 sugarbeet was 377, 269, and 144 times more resistant to primisulfuron, thifensulfuron, and chlorimuron, respectively, than susceptible S-2 sugarbeet. In contrast, R-1 sugarbeet was only 107, 76, and 57 times more resistant to primisulfuron, thifensulfuron, and chlorimuron, respectively, than S-1 sugarbeet, indicating at least a twofold difference in the magnitude of resistance between homozygous resistant and heterozygous resistant sugarbeet lines. ALS enzyme activity analysis were consistent with whole plant results. Thus, based on these two, maximum crop resistance can be obtained by developing homozygous resistant cultivars.

The effects of 14 herbicide treatments, tested in the establishment phase, were investigated on timothy productivity in two field experiments conducted at Melfort, Saskatchewan. Satisfactory weed control achieved with chlorsulfuron, metsulfuron, thifensulfuron, bentazon, diclofop plus bromoxynil, and MCPA/mecoprop/dicamba during the establishment year ensured good forage establishment which in turn caused a ‘residual effect’ for 1 to 3 yr increasing individual cut as well as cumulative forage dry matter yields significantly. In the stand established in 1985, when field pennycress (97 m-2) was the dominant weed in the seedling stage, these treatments increased yields 29 to 52% over a 3-yr period. In the stand established in 1986, when common lambsquarters (27 m-2) was the dominant weed, these treatments increased cumulative yields 24 to 44%. Sethoxydim and fenoxaprop, tested alone, caused significant crop injury in both experiments.

Chlorsulfuron, thifensulfuron, bromoxynil, 2,4-D, glyphosate, and a combination of 2,4-D plus glyphosate were applied on newly planted and established ‘Lemberger’ wine grape at 1/3, 1/10, 1/33, and 1/100 of the maximum labelled rate in wheat or fallow to simulate exposure to drifted herbicides. All herbicides produced symptoms on grape but the most severe symptoms were with 2,4-D and the least severe with bromoxynil. Newly planted grape was more sensitive to herbicides than established grape. Although established grape recovered from injury caused by all treatments except 2,4-D and the highest rate of chlorsulfuron and glyphosate, newly planted grape recovered only from lower rates of bromoxynil. All herbicides resulted in diagnostic symptoms, but other symptoms were very similar to those caused by other stresses.

A false cleavers population that survived treatment with triasulfuron/bromoxynil in 1996 was identified in central Alberta, Canada, in a field that had been treated with acetolactate synthase (ALS) inhibitors in 3 of the previous 6 yr. In greenhouse studies, this biotype was highly resistant to the ALS inhibitors triasulfuron, thifensulfuron/tribenuron, and sulfometuron and moderately resistant to imazethapyr; GR50, values were > 16, > 5, > 1.0, and 9.9, respectively. In addition, cross-resistance was identified to the auxin-type herbicide quinclorac (GR50 value > 6.7) but not to fluroxypyr (GR50 value 1) or MCPA/mecoprop/dicamba. Quinclorac had not been used previously in this field. Analysis of ALS extracted from the resistant biotype and a susceptible biotype from a nearby location indicated that resistance to ALS inhibitors was due to an altered target site with reduced sensitivity to a broad range of ALS inhibitors. The ALS I50 values for triasulfuron, metsulfuron, chlorsulfuron, thifensulfuron, and imazethapyr were 36, 34, 92, 96, and 14 times higher, respectively, for the resistant compared to the susceptible biotype. The mechanism of resistance to quinclorac is unknown. This is the first report of high-level herbicide resistance in this weed species.

It has been suggested that soil treated with a herbicide and subsequently carried by wind and deposited on plant foliage can cause crop injury. This study compared foliar uptake and translocation of herbicides applied to plants as an aqueous solution or in herbicide-treated soil. Leaves of 3-wk-old seedling alfalfa, grape, and pea were treated with 14C-labeled thifensulfuron, chlorsulfuron, glyphosate, 2,4-D, and bromoxynil. Significant amounts of all herbicides were absorbed by pea, alfalfa, and grape from the aqueous solutions, whereas very limited absorption occurred from herbicide-treated soil. Prolonged and multiple exposure to herbicide-treated soil did not increase herbicide uptake. High relative humidity enhanced herbicide absorption from aqueous solutions but not from herbicide-treated soil. All herbicides except bromoxynil were readily translocated in alfalfa, grape, and pea. Limited quantities of herbicides were absorbed from herbicide-treated soil by plant foliage, and this small amount is unlikely to cause crop damage.

Near isolines of ‘Nugaines’ winter Triticum aestivum that differed in height were planted with and without Aegilops cylindrica to determine the effect of plant height on competition against A. cylindrica. The isolines had either reduced height gene Rht 1, Rht 2, Rht 1 plus Rht 2, or neither Rht genes and averaged 79, 77, 51, and 101 cm tall, respectively, when grown with or without competition from A. cylindrica. Plants with fewer reduced height genes had the faster rates of height and weight gain, which are important traits for enhanced competitiveness. When growing in competition with A. cylindrica, the shortest isoline allowed the greatest amount of A. cylindrica seed production but did not have the lowest T. aestivum yield. However, when compared to the A. cylindrica-free control, the shortest isoline had the greatest percent yield loss. The tallest isoline reduced A. cylindrica seed production the most, and T. aestivum yield reduction due to A. cylindrica on a percent basis was the least when averaged over 2 yr. When competing against A. cylindrica, the tallest isoline did not always have the largest yield and yield parameters, and the shortest isoline did not always have the smallest yield and yield parameters. There is a cost to the T. aestivum plant to produce extra stem biomass that may reduce yield potential of taller plants and reduce the advantage gained by being taller than the surrounding weeds.

Field studies were conducted from 1991 to 1994 to investigate the effectiveness of reduced rates of bentazon in tank-mixtures with imazethapyr, thifensulfuron, or HOE 075032 for improved control of redroot pigweed and common lambsquarters in navy bean. Tank-mixtures of bentazon at 600 g ai/ha plus imazethapyr at 25 g/ha or HOE 075032 at 5 to 15 g/ha controlled both redroot pigweed and common lambsquarters. These rates represent an approximate 45% reduction in total amount of active ingredient needed to control these weeds compared with full label rates. Bentazon tank-mixtures with 2 or 4 g/ha of thifensulfuron controlled these weeds but caused severe injury and delayed crop maturity. In greenhouse studies, the GR25 for HOE 075032 in navy bean was > 90 g/ha, while in soybean it was 16 to 24 g/ha.

The effects of chlorimuron, imazaquin, imazethapyr, nicosulfuron, primisulfuron, and thifensulfuron were evaluated on a population of smooth pigweed in Painter, VA with no history of treatment with acetolactate synthase (ALS)-inhibitor herbicides. Imazethapyr and nicosulfuron gave the greatest smooth pigweed control, and subsequently were used in field and greenhouse studies to investigate susceptibility of smooth pigweed and livid amaranth populations to ALS-inhibitor herbicides. Approximately 5 million smooth pigweed plants from Painter were treated with imazethapyr or nicosulfuron from 1992 to 1994 and no ALS-inhibitor-resistant plants were identified. In the greenhouse, the response of smooth pigweed from Painter, VA, Marion, MD, and Oak Hall, VA and livid amaranth from Warren County, NJ to imazaquin or imazethapyr and nicosulfuron was investigated. Smooth pigweed from Marion and Oak Hall and livid amaranth from NJ had histories of treatment with ALS-inhibitors. Painter smooth pigweed control was 81 to 97% by imazethapyr and nicosulfuron while control of the Marion and Oak Hall populations was 3 and 18% by imazaquin at 560 and 1120 g ai/ha, respectively, and control by nicosulfuron at 35 g ai/ha was 50 to 73%. Control of livid amaranth from Warren County, NJ was 8 to 15% by imazethapyr at 560 g ai/ha, and was 30 to 58% by nicosulfuron at 35 g/ha.

Herbicide systems consisting of PRE, early POST, and late POST options arranged factorially were compared for control of sicklepod in narrow-row soybean with modified acetolactate synthase (ALS) (E.C.4.1.3.18). Other weeds present included common cocklebur and mixed infestations of entireleaf, ivyleaf, pitted, and tall morningglories. PRE options were alachlor or alachlor plus metribuzin plus chlorimuron. Early POST options included chlorimuron, chlorimuron plus thifensulfuron, and no herbicide applied 3 wk after planting. Late POST options were chlorimuron and no herbicide applied 5 wk after planting. POST herbicides were more effective than PRE herbicides on all weeds. Chlorimuron and chlorimuron plus thifensulfuron applied early POST were equally effective on these weeds and usually more effective than chlorimuron applied late POST. There was no advantage of two POST applications compared with a single early POST application. Greatest net returns were obtained in systems using only early POST herbicides. There was no economic advantage from using metribuzin plus chlorimuron PRE in systems that included an early POST herbicide.

‘Vernal’ alfalfa response was evaluated when chlorsulfuron, thifensulfuron, 2,4-D, glyphosate, bromoxynil, and selected combinations of those herbicides were applied at rates simulating spray drift during the fourth trifoliolate leaf stage following the first cutting in 1990 and 1991. The order of phytotoxicity was 2,4-D > chlorsulfuron > thifensulfuron > glyphosate > bromoxynil. By the end of each growing season, alfalfa had recovered from injury caused by all herbicides except the highest rates of 2,4-D and 2,4-D plus glyphosate. The alfalfa stand was reduced only by 2,4-D and 2,4-D plus glyphosate. All herbicides caused characteristic symptoms, but some specific symptoms were similar among different herbicides or resembled symptoms caused by disease, mineral imbalance, and adverse weather conditions.

Experiments were conducted to evaluate imazaquin and selected herbicides for POST control of wild garlic. At the same dosages, imazaquin applied in late November/early December (late fall) controlled wild garlic better than when applied in early March (late winter) when assessed in May. Imazaquin provided control equivalent to 1120 g ae ha–1 2,4-D plus imazaquin. Imazapyr, imazamethabenz, or imazethapyr at 280, 1120, or 560 g ai ha–1, respectively, provided control equivalent to 560 g ai ha–1 imazaquin. Thifensulfuron at 70 g ai ha–1 provided better control applied in late winter (85%) than late fall (45%). All herbicides consistently provided better control than 2,4-D plus mecoprop plus dicamba, except thifensulfuron applied in late fall, at the final evaluation. Common bermudagrass growth in early spring was delayed 2 mo by ≥ 560 g ha–1 imazaquin, 70 g ha–1 thifensulfuron, imazethapyr, and imazapyr or 2,4-D plus mecoprop plus dicamba applied in late winter. Imazamethabenz did not delay common bermudagrass growth. Late fall applications of 1120 g ha–1 imazaquin did not delay growth while ≥ 560 g ha–1 imazethapyr delayed common bermudagrass growth through April.

Greenhouse and laboratory studies were conducted to determine the effects of bentazon on the crop safety, efficacy, foliar absorption, and translocation of thifensulfuron when it is applied to soybean, velvetleaf, and common lambsquarters. A metabolism study was conducted on soybean. Thifensulfuron applied at 1.1 g ai ha−-1 with 28% urea ammonium nitrate (2.5% v/v) and BAS-0904805 (Dash) adjuvant (0.63% v/v) reduced the growth of velvetleaf and common lambsquarters by an average of 91 and 84%, respectively. The addition of 420 g ai bentazon ha−-1 had no effect on thifensulfuron efficacy in the weed species. Soybean dry weights were decreased by 58% when thifensulfuron was applied at 2.2 g ha−-1 but were decreased by only 36% when bentazon, at 420 g ha−-1, was added. In the absorption study, the addition of bentazon reduced foliar absorption of 14C-thifensulfuron into velvetleaf and common lambsquarters 8 and 24 h after treatment (HAT), but absorption into soybean was not affected. Bentazon reduced the translocation of 14C from thifensulfuron out of the treated leaves of velvetleaf and common lambsquarters by at least 16 and 11%, respectively, beyond 24 HAT. Soybean translocated 18, 29, and 26% of absorbed 14C out of the treated leaflets 24, 72, and 168 HAT, respectively. These translocation values were reduced to 7, 12, and 11%, respectively, when bentazon was tank-mixed with thifensulfuron. Soybean metabolized 35% of recovered 14C-thifensulfuron by 24 HAT. Addition of bentazon did not change this level of metabolism. These studies suggest that the physiological basis for the decrease of soybean injury from thifensulfuron, when it is tank-mixed with bentazon, is decreased thifensulfuron translocation.

Extensive field and greenhouse studies were done to characterize varietal response of three recently commercialized sulfonylurea corn herbicides: nicosulfuron, primisulfuron, and thifensulfuron. Most of the 94 varieties tested were highly tolerant to these herbicides. The 37 inbreds represented all major inbred families now used in hybrid seed production as well as several sensitive experimentals. Twenty-one defined hybrids from these inbreds as well as 36 commercially coded hybrids were also tested. Sensitive inbreds produced tolerant hybrids when crossed with tolerant inbreds. Sensitive hybrids occurred when both parents were sensitive. Genetic analysis of sensitive by tolerant crosses showed that sensitivity is controlled by a single recessive gene. Nicosulfuron had the widest corn safety margin and fewest sensitive varieties. Dose response analysis showed varieties can vary more than 40 000-fold in sensitivity. Only corn varieties with the AHAS-modified XA-17 gene showed any change in enzyme sensitivity. This gene overcame sensitivity to sulfonylureas, even when the organophosphate insecticide terbufos was present. Thus, breeders have three options to eliminate sulfonylurea sensitivity: backcross sensitive inbreds with tolerant, always use at least one tolerant hybrid parent, or use the XA-17 gene.

The antidotes dichlormid, MON-4660, CGA-154281, R-29148, and MON-13900 were tested in the greenhouse to protect Zea mays L. (corn) against RPA 201772 injury. High rates of RPA 201772 injured four Z. mays hybrids > 30%. R-29148 and MON-13900 were the most effective of the five antidotes evaluated. R-29148 applied at rates ⩾ 45 g ha−1 provided excellent protection against RPA 201772 injury and also prevented injury to Z. mays from diketonitrile, the active metabolite of RPA 201772. In laboratory studies, R-29148 did not alter absorption of 14C-RPA 201772 from soil; however, R-29148 significantly enhanced the rate of RPA 201772 metabolism and inactivation in Z. mays. The mixed function oxidase inhibitor piperonyl butoxide (PBO) increased RPA 201772 injury on all hybrids. These results demonstrate that Z. mays tolerance to RPA 201772 can be enhanced with the use of antidotes such as R-29148 and MON-13900, that R-29148 protects Z. mays from RPA 201772 and diketonitrile by the enhancement of metabolism, and that oxidative reactions may be involved in the metabolism of RPA 201772 in Z. mays.