A total of 90 cows from three commercial farms were used to evaluate the relationship between subclinical mastitis and clinical mastitis and thermal nociceptive threshold. Milk strips from all udder quarters were tested for clinical mastitis with visual inspection of milk and udder alterations and for subclinical mastitis using California Mastitis Test. Milk yield was recorded, milk was sampled and further analyzed for somatic cells count (SCC). Cows were considered healthy when SCC<200 000 cells/ml and no visual alterations in milk and/or udder, with mild subclinical mastitis when SCC>200 000 cells/ml and no visual alterations in milk and/or udder, with moderate subclinical mastitis when SCC>500 000 cells/ml and no visual alterations in milk and/or udder and with clinical mastitis when visual alterations in milk and/or udder were detected. Nociceptive threshold was evaluated with the thermal threshold meter apparatus applied to the rear legs. Thermal threshold (TT) decreased when we compared healthy cows with cows presenting clinical mastitis and tended to decrease when we compare healthy cows with those with moderate subclinical mastitis. TT was lower at the ipsilateral rear leg compared with the contralateral leg to the infected mammary gland. TT linearly decreases as log10SCC increased and it showed sharp decrease as log10SCC exceed the value of 6.4. Increase in one unit of log10SCC increased the odds of low thermal threshold (lower than 55.8°C). Subclinical mastitis might be a welfare issue as it tended to decrease nociceptive thermal threshold.

Twenty-five weaned 35-day-old piglets were used in a 35-day growth experiment to evaluate the effect of inclusion of chicory and ribwort forage in a cereal-based diet on growth performance, feed intake, digestibility and shedding of faecal coliform bacteria. A total of seven experimental diets were formulated, a cereal-based basal diet (B), and six diets with inclusion of 40, 80 and 160 g/kg chicory (C40, C80 and C160) or ribwort (R40, R80 and R160). Piglets had ad libitum access to feed and water throughout the experiment. Three and five weeks post-weaning faeces samples for determination of digestibility were collected once a day for five subsequent days. Additional faeces samples for determination of coliform counts were collected at days 1, 16 and 35 post-weaning. Piglets fed diet R160 had the lowest average daily feed intake (DFI) and daily weight gain (DWG), and differed (P < 0.05) from piglets fed diets B, R40 and R80. There were no differences in DFI and DWG between the chicory diets and diet B. Inclusion of chicory or ribwort had a minor negative impact on the coefficient of total tract apparent digestibility (CTTAD) of dry matter, organic matter and crude protein, whereas inclusion of both chicory and ribwort resulted in higher CTTAD of non-starch polysaccharides and neutral detergent fibre (NDF). The CTTAD of arabinose were higher for diets C160 and R160 than for diet B (P < 0.05), and the CTTAD of uronic acid was higher for diets C40, C80, C160, R80 and R160 than for diet B (P < 0.05). Age affected the CTTAD for all parameters (P < 0.05) except for NDF, with higher values at 5 than at 3 weeks post-weaning. The coliform counts decreased with increasing age (P < 0.05), but was not affected by treatment. The results indicate that inclusion of up to 160 g/kg of chicory do not negatively affect performance, whereas high inclusion of ribwort have a negative impact on feed consumption and consequently on growth rate. Both herbs have a higher digestibility of fibre compared to cereal fibre. Chicory and ribwort are both promising as feedstuffs to weaned piglets, but the low palatability of ribwort limits the inclusion level.

Phyto-oestrogens are believed to have a range of beneficial effects on predominant Western diseases. A few studies on phyto-oestrogens in milk exist and show that the composition can be affected by feeding. Therefore, the aim was to study how feeding of lucerne and grass/clover silages (GCSs) affects the concentration of phyto-oestrogens in bovine milk. Sixteen Danish Holstein cows were assigned to a 4 × 4 latin square design with four cows per treatment per period of 3 weeks. The four treatment diets were lucerne silage (LS), 2/3 lucerne silage and 1/3 maize silage (2/3LS), 1/3 lucerne silage and 2/3 maize silage (1/3LS) and GCS. Milk was collected at the end of each period and feed samples on day 6, 13 and 20 in each experimental period. Milk and pooled feed samples were analysed for the concentration of isoflavones, coumestans and lignans. The content of isoflavones was higher and the content of coumestrol lower in the GCS diet than in LS, 2/3LS and 1/3LS diets. For the LS, 2/3LS and 1/3LS diets, the concentration of coumestrol and secoisolariciresinol increased with the proportion of lucerne while the concentration of isoflavones was similar across the diets. The concentrations of the formononetin, daidzein and equol in the milk were significantly higher for the GCS diet than for the LS, 2/3LS and 1/3LS diets. In particular, the concentration of equol was 62–291 times higher for GCS. The concentration of coumestrol was significantly lower for the GCS diet compared to the LS, 2/3LS and 1/3LS diets. No pattern for the concentration of lignans was observed. In conclusion, a high concentration of isoflavones, particularly equol, and a low concentration of coumestrol were measured in the milk from the GCS diet. In contrast, a low concentration of isoflavones and a high concentration of coumestrol were measured in the milk from the LS, 2/3LS and 1/3LS diets. However, the concentration of phyto-oestrogens in bovine milk is low compared to food sources rich in phyto-oestrogens but the high concentration of equol could possibly be of therapeutic importance.

Alanyl-glutamine (Ala-Gln), a highly soluble and stable glutamine dipeptide, is known to improve gut integrity and function. The aim of this study was to evaluate whether dietary Ala-Gln supplementation could improve growth performance, intestinal development and digestive-absorption function in weaned piglets. A total of 100 purebred Yorkshire piglets weaned at 21 days of age were assigned randomly to four dietary treatment groups and fed a basal diet (control group) or a basal diet containing 0.15%, 0.30% and 0.45% Ala-Gln, respectively. Compared with the control group, piglets fed the Ala-Gln diets had higher average daily gain and lower feed : gain and diarrhea rate (P < 0.05). Moreover, dietary Ala-Gln supplementation increased villous height and villous height : crypt depth ratio in duodenum and jejunum (P < 0.05), as well as the activities of maltase and lysozyme in jejunum mucosa (P < 0.05). In addition, a decrease in serum diamine oxidase activity and crypt depth in duodenum and jejunum was observed in piglets fed the Ala-Gln diets (P < 0.05). Serum cytosolic phospholipase A2 (cPLA2) concentration and gene expression of cPLA2, Na+-dependent glucose transporter 1, glucose transporter 2 and peptide transporter 1 in jejunum were increased by feeding Ala-Gln diets relative to control diet (P < 0.05). These results indicated that feeding Ala-Gln diet has beneficial effects on the growth performance of weaned piglets, which associated with maintaining intestinal morphology and digestive-absorption function.

Keeping cattle inside on concrete slatted floors can be detrimental to their health and behaviour and is also costly. Therefore, 22 steers of the Swedish Red and White Dairy breed were used to investigate the effect of wintering outdoors on growing cattle. The steers had a mean weight of 310 kg at the onset of the study. During winter, 11 ‘indoor’ steers were housed in pens with concrete slatted floors and 11 ‘outdoor’ steers were kept in a field with access to a shelter and trees. From the end of April until slaughter in September, both groups grazed together. During winter, all steers were given clover silage ad libitum. They were weighed every month. The behaviour of the outdoor steers was recorded from November to the end of March. Outdoor temperature, wind speed and solar radiation were measured continuously. A heated model was used in order to calculate the climatic energy demand. The steers were never observed to shiver. They were not observed to use the shelter during daytime. The most frequently observed behaviour was ‘eating’, followed by ‘standing’. The lower the temperature, the more time the steers were observed lying down (P < 0·01). ‘Moving’ increased with increasing temperature (P < 0·05) as well as with increasing wind speed (P < 0·01). During the grazing period following the experiment, the former outdoor steers grew significantly (P < 0·05) better than the former indoor steers. However, there was no significant difference in overall growth rate from start to slaughter. This study suggests that the winter climate in this part of Sweden (latitude 60°N) did not affect in a negative way the welfare or the growth rate of steers kept outdoors.

Lactobacillus rhamnosus GG (LGG) is a probiotic for humans and is normally not found in pigs; however, it has been shown to protect the human-derived intestinal Caco-2 cells against the damage induced by an important intestinal pathogen, enterotoxigenic Escherichia coli F4 (ETEC). An experiment was conducted to test whether the dietary addition of LGG improves the growth and health of weaned pigs when orally challenged by E. coli F4. Thirty-six pigs were weaned at 21 days and assigned to a standard weaning diet with or without 1010 CFU LGG (ATCC 53103) per day. The pigs, individually penned, were orally challenged with 1.5 ml of a 1010 CFU E. coli F4 suspension on day 7 and slaughtered on day 12 or 14. With the addition of LGG, the average daily gain and the average daily feed intake were reduced after the challenge with ETEC and for the entire trial (P < 0.05). The average faecal score tended to worsen from day 11 to the end of the trial and the concentration of ETEC in the faeces tended to increase (P = 0.07) with the LGG supplementation. The counts of lactic acid bacteria, enterobacteria and yeasts in the colonic digesta were not affected. The pH values in ileal, colonic and caecal digesta, and the small intestine size were also unchanged. Regardless of the site of measurement (duodenum, jejunum or ileum), a trend of decreased villus height was seen with LGG (P = 0.10). Crypt depth and villus to crypt ratio were unchanged by the diet. A gradual increase of total seric IgA was seen after 1 week and after the challenge, in the control (P < 0.05), but not in the treated group. After the challenge, the LGG reduced the total IgA in the blood serum (P < 0.05), v. the control. The total IgA in the saliva and in the jejunum secretion were not affected by the diet. The F4-specific IgA activity was not affected by the diet at all the samplings. Our result shows that, the administration of LGG do not prevent or reduce the detrimental effect of the E. coli F4 infection on the growth performance and health status of weaned piglet.

The rapid shift to high-grain (HG) diets in ruminants can affect the function of the rumen epithelium, but the dynamic changes in the composition of the epithelium-associated (epimural) bacterial community in sheep still needs further investigation. Twenty male lambs were randomly allocated to four groups (n = 5). Animals of the first group received hay diet and represented a control group (CON). Simultaneously, animals in the other three groups (HG groups) were rapidly shifted to an HG diet (60% concentrate)which continued for 7 (HG7), 14 (HG14) and 28 (HG28) days, correspondingly. Results showed that ruminal pH dramatically decreased due to the rapid shift to the HG diet (P <0.001), while, the concentrations of butyrate (P <0.001), lactate (P = 0.001), valerate (P = 0.008) and total volatile fatty acids (P = 0.001) increased. Diversity estimators showed a dramatic decrease after the shift without recovering as the HG feeding continued. The principal coordinates analysis showed that CON group clustered separately from all HG groups with the presence of significant difference only between HG7 and HG28 (P = 0.034). The non-parametric multivariate analysis (npmv R-package) deduced that the primary significant differences in phyla and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt)-predicted Kyoto Encyclopedia of Genes and Genomes (KEGGs) was attributed mainly to the diet composition (P <0.001, P = 0.001) compared to its application period (P = 0.140, 0.545) which showed a significant effect only on the genus (P = 0.001) and the operational taxonomic units (OTUs) level (P = 0.011). The Kruskal–Wallis test deduced that six phyla showed a significant effect due to the shift in diet composition. At the genus level, HG feeding altered the abundance of 12 taxa, four of which showed a significant variation due to the duration of the HG diet application. Similarly, we found that 21 OTUs showed significant variations due to the duration of the HG diet application. Furthermore, the genes abundance predicted by PICRUSt revealed that the HG feeding significantly affected seven metabolic pathways identified in the KEGG. Particularly, the abundance of gene families associated with carbohydrates metabolism were significantly higher in HG feeding groups (P = 0.027). Collectively, these results revealed that the rapid transition to an HG diet causes dramatic alterations in ruminal fermentation and the composition and function of ruminal epithelium-associated microbiome in sheep, while, the duration of the HG diet application causes drastic alterations to the abundance of some species.

Twin lambs grazing with their dams at herbage allowances of 30 or 60 gdry matter per kg live weight per day (low and high, respectively) were weaned at 6, 8, 10 or 14 weeks of age. After weaning, the lambs were given a herbage allowance of 160 g dry matter per kg live weight per day.

Ewes on the low allowance ate less herbage, produced less milk and lost more weight than those on the high allowance. The lambs on the low allowance were lighter at weaning than those on the high allowance (137 v. 168, 16·2 v. 20·5, 17·9 v. 24·2 and 21·9 v. 30·4 kg at 6, 8, 10 and 14 weeks respectively).

Lambs weaned from ewes on the low allowance grew more slowly than the unweaned lambs when removed at 6 weeks, but not when weaned at 8 and 10 weeks of age. Weaning lambs from ewes on the high allowance reduced their growth rates at all weaning ages, despite the weaned lambs being grazed at an allowance which would not limit their intake of herbage. The intake of herbage per unit live weight by weaned lambs reached its maximum at approximately 8 weeks of age. After weaning, intake was higher for lambs from ewes on the low allowance than for those on the high allowance (38 v. 33 g organic matter per kg live weight per day). Lambs slaughtered at 17 weeks of age were heavier from the high allowance than from the low allowance. However, after 17 weeks of age the remaining lambs from the low allowance grew more rapidly than those from the high allowance, and when slaughtered at 25 weeks of age had similar weights. Treatments had no effect on the weights of the body components or carcass dimensions independent of their effect on empty-body weights at slaughter.

Forty-two weaned suckled Charolais-cross steers were used to measure changes in body composition during compensatory growth in growing cattle. Six cattle were slaughtered initially and the remaining 36 allocated to either a low level of feeding to 350 kg live weight followed by a high level (LH) or a high level of feeding throughout (HH). Above 350 kg live weight, food intake on both treatments was the same at any given live weight. Six cattle were slaughtered from each treatment at 350, 400 and 450 kg live weight. From initial live weight (259 kg) to 350 kg, live-weight gains were 0·45 and 0·78 kg/day for the LH and HH treatments respectively (P < 0·001). From 350 to 400 kg live weight, live-weight gains were 1·35 and 0·98 kg/day (P < 0·01) for the LH and HH cattle respectively, while from 400 to 450 kg live weight there was no significant difference (1·38 v. 1·20 kg/day). The LH cattle contained less fat in the empty body than the HH cattle at 350 kg (118 v. 153 g/kg; P < 0·05) and 400 kg live weight (117 v. 169 g/kg; P < 0·01), but at 450 kg there was no significant difference between treatments. From 350 to 400 kg live weight the composition of the empty body-weight gain was 663 g water, ' 108 g fat and 216 g protein per kg in the LH cattle and 422 kg water, 311 g fat and 173 g protein in the HH cattle. From 400 to 450 kg live weight the equivalent figures were 491, 291, 156 g/kg for the LH cattle and 744, 67 and 203 g/kg for the HH cattle. The results demonstrate that following a period of food restriction the empty body-weight gain of cattle initially comprises increased proportions of protein and water and a reduced proportion of fat compared with unrestricted cattle when both are given the same amount of food and compared at the same weight. There then follows a second phase in which the proportion of fat increases and the proportions of protein and water decrease.

Data were collected on 19 high-producing Holstein dairy cows from day 20 post partum until they were either determined pregnant or reached 150 days post partum. Blood metabolites (progesterone (P4), glucose, beta-hydroxybutyrate (BHBA) and free fatty acids (FFA)) were related to the interval from parturition to first luteal phase, first visual oestrus and conception. Prior to data analysis, cows were divided into two groups based on their 120-day milk production and designated as below herd average (BHA; no. = 9) or above herd average (AHA; no. = 10). Average 120-day milk productions for BHA, AHA and herdmates (no. = 56) were 3932 (s.e. 157), 4841 (s.e. 60) and 4353 (s.e. 96) kg, respectively. Days from parturition to first luteal phase were not different between the BHA and AHA groups (31·7 (s.e. 1·7) and 31·5 (s.e. 6·9), respectively). In contrast, days from parturition to first visual oestrus and to conception oestrus were less for the BHA group than for the AHA group (46·8 (s.e. 4·6) and 76·9 (s.e. 7·5) v. 67·7 (s.e. 5·2) and 102·5 (s.e. 9·2) days, respectively (P < 0·05)). Further, average glucose concentration from day 20 to first luteal phase was higher (P < 0·05) for the BHA group than for the AHA group (64·1 (s.e. 1·6) v. 58·4 (s.e. 1·6) mg/dl). From day 20 to first visual oestrus, plasma glucose concentration increased while BHBA and FFA declined; all three metabolites then remained relatively constant until conception. These data suggest that as the amount of milk a dairy cow produces increases, the interval from parturition to first visual oestrus and conception increases. These data are consistent with a suppression of oestrus behaviour rather than with a delayed interval to ovarian cyclicity.

New methods of design and analysis for mixed grazing experiments are proposed and compared with previously reported designs. In general, with previous designs the effects of mixed grazing per se were confounded with other factors. The concepts of equality of grazing pressure and of livestock equivalents are examined and criticized when used as starting points for mixed grazing experiments. Two year's results of a mixed grazing experiment with cattle and sheep are analysed and provide evidence that mixed grazing is beneficial to both species in terms of production per animal. A simple economic analysis shows how the price ratio between the value per unit weight gain of the two species can be used to decide on the area of financial benefit from mixed- as opposed to single-species enterprises.

Thirty young male sheep were given air-dried foliage of Acacia cyanophylla Lindl. ad libitum and 400 g oat hay. Animals were divided into five equal groups each of which was supplemented with either 300 g concentrate with (CPEG) or without (C) 20 g polyethylene glycol 4000 (PEG) or urea-containing feedblocks with (BUPEG) or without (BU) PEG. One other treatment was PEG-containing feedblock without urea (BPEG). PEG was used to preferentially bind A. cyanophylla condensed tannins (CT). Feedblocks (BU, BPEG and BUPEG respectively) were made from olive cake (395, 376 and 353), wheat bran (263, 250 and 235), wheatfeeds (132, 125 and 118), quicklime (88, 83 and 78), salt (44, 42 and 39), mineral and vitamin supplement (12, 10 and 10), urea (66, 0 and 59) and PEG (0, 114 and 107 g/kg). These diets were used in growth (60 days) and metabolism (7 days) studies. Urea-containing feedblocks, as compared with concentrate, were higher in crude protein (CP; 235 v. 116 g/kg DM) and lower in metabolizable energy (6 v. 10 MJ/kg DM). The type of supplement (i.e. concentrate v. feedblocks) had no effect (P > 0·05) on A. cyanophylla DM intake, CP and neutral-detergent fibre (NDF) digestibilities and nitrogen (N) retention. However, concentrate-containing diets exhibited higher DM and organic matter (OM) digestibilities (P < 0·05), urinary excretion of allantoin (P < 0·05), microbial N supply (P < 0·001) and average daily gain (P < 0·001). PEG supply increased DM intake of A. cyanophylla, CP digestibility, N retention, allantoin voided in urine, microbial N supply and lamb growth. PEG-containing feedblocks enriched (BUPEG) or not (BPEG) with urea had similar effect on A. cyanophylla intake, DM, OM and NDF digestibilities, allantoin in urine, microbial N supply and average daily gain. These findings suggested that urea inclusion in feedblocks are not necessary and thus the feeding cost may be reduced. The higher amount of microbial N and daily gain in lambs given concentrate as compared with those on feedblock-supplemented diets was ascribed to the level of energy which was low in the latter diets. Enrichment of feedblocks with energy-rich ingredients such as molasses or cactus fruits could be a solution. This work confirmed that tannins depressed the nutritive value of this shrub species and lamb growth. However, the reduction was not drastic in the absence of PEG in the diet. Feedblock diet (BU) matched maintenance requirements of lambs while lambs on concentrate-supplemented diet (C) grew at a rate of 78 g/day. The choice between these two supplements will depend upon farm conditions and objectives.

In a new ranching area of Uganda recently cleared of trypanosomiasis, with a high level of disease control, adequate water supplies and extensive grazing on free range, three dam breeds, the indigenous Ankole and Zebu, and the Boran from Kenya, were mated to Boran, Angus and Red Poll sires.

Under artificial insemination, at least 10 % fewer cows were detected on heat than under natural service, and 15% fewer artificially inseminated cows actually produced calves than did naturally served cows. The introduced Boran females were not inferior in calving percentage to the indigenous Ankole and Zebu.

Relationships between dam body weight and progeny performance were investigated. The introduced Boran breeding cows were able to maintain their body weight as least as well as the indigenous Ankole and Zebu cows. While cows which calved regularly and reared their calves could not maintain body weight, it was found that heavier cows conceived at a higher rate than lighter cows, cows heavier at parturition weaned heavier calves than lighter cows, and cows that reared heavier calves lost more, or gained less, body weight than cows that reared lighter calves. It was therefore indicated that investigations into the reduction of lactation stress on cows by such methods as earlier weaning of calves, mating at the most suitable time of year, and possible nutritional supplementation at a stage in the cycle, should be commenced.

1. The four systems of rearing dairy heifer calves, described in Part 1 of this series are now studied for their effect on absolute and relative growth rates to maturity.

2. Data on live-weights and measurements and the percentages of mature size attained by these at 44, 80, 104, 132, 182, 260 and 312 weeks of age are tabulated.

3. In all groups the measurement to reach maturity earliest was circumference of metacarpus followed in order by length of back, height at withers and width of hooks.

4. By 6 years of age all groups had attained approximately the same body size. Maturity in the LL group in terms of four skeletal measurements was delayed by only 9 months, and in the case of HL and LH animals by 5 and 4 months respectively.

5. All groups showed the same general pattern of relative growth but the effect of restricted feeding was to increase at the younger ages the difference i n percentage of mature size between early and late maturing measurements. After 44 weeks of age the rate of growth was most rapid in those measurements which were furthest from maturity then.

6. The rate of tissue deposition was markedly increased in high plane animals during early pregnancy compared with low plane. Evidence is presented to show that much of this was lost during lactation.

7. At first oestrus, animals in all four treatment groups had reached the same percentage of mature size for each body dimension (except for length of back in the LL group).

8. From data on 5 animals it is shown that although growth in height at withers and length of back ceased by 6 years of age there was slow but continuous growth in live-weight, middle and heart girths and width of hooks to 9 years of age.

The reproductive performance and food conversion efficiency ofyoung female pigs (gilts) destined for slaughter after weaning their first litter were investigated using four treatment groups of 19 Landrace × (Landrace × Large White) gilts. In two treatment groups gilts were mated at pubertal heat and suckled for 5 to 11 and 35 to 42 days respectively. These gilts were slaughtered following weaning. A third group of gilts were conventionally mated at third oestrus and suckled for 35 to 42 days. These animals acted as controls for reproductive performace. The fourth treatment group consisted of unmated gilts, slaughtered at 118 kg live weight, which served as controls for food conversion efficiency, growth rate and carcass measurements.

There were no significant differences in either the number or weight of piglets born in any of the treatment groups. The food conversion efficiency and growth rate of the mated gilts was significantly (P< 0·001) worse than that of the unmated gilts marketed at heavy weights. Of the mated gilts those mated at puberty and weaned at 35 to 42 days had the best overall feed conversion efficiency (4·56: 1) between the start of the experiment and weaning and required less food per piglet reared to 6 weeks of age than the gilts mated at third heat.

The aim of this experiment was to study the effects of dietary energy level and source on foetal growth and survival and mobilization of body stores in concurrently pregnant and lactating primiparous rabbit does. Does were given a moderate-energy diet (≍9·9 MJ digestible energy (DE) per kg dry matter (DM); group M, no. = 23) or a high-energy diet (-12·2 MJ DE per kg DM). In this latter case, additional energy came from starch (group S, no. = 23) or starch and fat (group F, no. = 22). Primiparous rabbit does were mated within 12 h after parturition and were slaughtered on day 28 of gestation-lactation. During the first 21 days, the DE intake and milk production were higher in the group F than in the groups S and M (P < 0·05). On day 28 of pregnancy, the weight of adipose tissues and liver, as well as the lipid content of the carcass, were higher in the groups S and F than in the group M (P < 0·05). Foetal survival and weight were similar in the three groups. These results indicate that increased energy level of the diet did not improve foetal survival or growth in concurrently pregnant and lactating primiparous does. On the contrary, it could reduce the mobilization offat stores, especially if the energy source is starch.

Silages were made from the primary growth of perennial ryegrass (Lolium perenne) or red clover (Trifolium pratense) and were given ad libitum to 52 British Friesian cows, together with 70 kg dry matter per day of a barley/ground maize/soya bean supplement in 2 years. Twenty of the cows were used in both years, providing a total of 72 observations. Three treatments were imposed during weeks 3 t o 20 of lactation: red clover silage, (RC/RC); red clover silage, weeks 3 to 10, grass silage weeks 12 to 20 (RC/PRG); grass silage (PRG/PRG).

Apparent digestibility of the gross energy assessed in lactation weeks 14 and 15 was slightly lower for red clover than for perennial ryegrass (0·683, 0·706 and 0·701 for treatments RC/RC, RC/PRG and PRG/PRG respectively). There was no effect of previous treatment on the digestibility of perennial ryegrass diets (RC/PRG v. PRG/PRG). Cows given red clover silage consumed proportionately 1·22 of the dry matter and 1·09 of the digestible energy ingested by those given perennial ryegrass. The intake of perennial ryegrass in mid lactation was similar, irrespective of early lactation treatment.

During weeks 3 to 20, cows given red clover yielded 1·9 kg more milk than did those given perennial ryegrass (P < 0·01). However, this milk had a significantly lower concentration of fat (P < 0·01; 37·2 and 41·2 g/kg for treatment RC/RC and PRG/PRG respectively). In mid lactation the cows that had received red clover silage in early lactation (RC/PRG) had the lowest yield of milk, milk fat, protein and lactose and the difference between this treatment and PRG/PRG was significant for milk and milk fat yields (P < 0·05).

Red clover silage rather than perennial ryegrass silage of similar digestibility resulted in higher silage intake and higher yields of milk, milk protein and lactose. However, the strategic use of red clover silage to increase yield in early lactation did not induce positive residual effects in mid lactation; rather, there was evidence of a negative effect on milk and milk-fat yield.

Diet selection, live-weight changes and productivity per hectare of two breeds of goats (Cashmere and local Celtiberic) managed in natural vegetation dominated by heather, and the effect of stocking rate (high or low) in the Cashmere breed, were studied during four grazing seasons. Breed had a significant (P < 0.01) effect on the available biomass and its components over the experimental period as a consequence of the differences in dietary components selected by the animals. Local goats produced a greater reduction in short and tall heather and in the percentage of leaves in the tall heather. Live-weight changes were significantly (P < 0.001) different between breeds. Over all experimental years Cashmere goats maintained weight under both low and high stocking rate (4 and − 1 g/day respectively), while local Celtiberic goats lost 30 g/day.

Across all grazing seasons and experimental years stocking rate did not significantly affect individual live-weight changes of Cashmere goats. Productivity per hectare did not differ significantly (P>0.05) between high ( − 0.6 kg/ha) and low stocking rate treatments (4.1 kg/ha). However it was significantly (P < 0.001) worse for the local breed treatment ( − 51 kg/ha).

As a consequence, in these vegetation communities with low abundance and poor quality of herbaceous plants it will be impossible to develop sustainable systems from the animal production point of view, and their management would need to be integrated during the grazing season by alternating with vegetation communities with better quality.

A total of 3200 milk samples from Holstein and Jersey cows were analysed for free glucose and glucose-6-phosphate (G6P) by an enzymatic-fluorometric method that requires no pre-treatment. The cows were primiparous as well as multiparous, and samples were taken throughout the entire lactation period. In addition, lactose, protein, fat, citrate and β-hydroxybutyrate were determined and comparisons between these variables were made. Data were analysed using GLM model for the effect of parity, breed, time from last milking and stage of lactation on variations in parameters in milk. Pearson’s correlations were generated between milk variables. P<0.05 was considered significant. Concentration of free glucose and G6P were on average 331 and 81 μM, respectively. Time from last milking (stay in the gland cistern) did not increase the concentration of these monosaccharides, indicating that they are not hydrolysis product from lactose post secretion, but rather reflecting the energy status of the mammary epithelial cells pre-secretion. Wide variation in range of these metabolites, that is, from 90 to 630 μM and 5 to 324 μM, for glucose and G6P, respectively, was observed. During the first 21 weeks in milk, free glucose increased whereas G6P decreased. Concentration of free glucose in milk is greater for primiparous than multiparous cows and greater for Holstein than Jersey cows. Concentration of G6P was not affected by parity or breed. The use of free glucose and G6P as indicators of physiological conditions and risk of disease is warranted for use as potential biomarkers for in-line surveillance systems on-farm.

Intramuscular fat (IMF) content depends on sex, genotype and diet and varies with pig growth. The aim of the present work was to determine the evolution of IMF by genotype-sex, muscle and muscle location, to determine relationships between IMF content of different muscles and to predict IMF in live pigs with computed tomography (CT). For this purpose, 155 pigs of seven combinations of genotype-sex were CT scanned and slaughtered at 70, 100 and 120 kg. From the carcasses, fat thickness was measured at several locations along the midline. Loin samples from three anatomical positions (between the eighth and ninth last ribs, between the third and fourth last ribs and between the third and fourth lumbar vertebrae) and three ham muscles (biceps femoris, semimembranosus and gluteus medius) were extracted, weighed and IMF was determined with near-IR equipment. From CT images, the distribution of volume by Hounsfield value (unit related with the density) was obtained for each muscle and anatomical location. Marbling was evaluated in the three loin locations. The effects of genotype-sex and live weight and their interaction were included in the statistical model. For prediction of IMF with CT images, partial least square regression was used. The results show differences in IMF content by genotype-sex and muscle. In general, the most cranial part of the loin presented higher IMF content, as well as the biceps femoris muscle of the ham. Depending on the genotype-sex, IMF content increased during all growth or increased until 100 kg and then became constant. Correlation coefficients between IMF content by muscle/location were between 0.74 and 0.83 within loin locations and between 0.53 and 0.70 for ham muscles. Correlation coefficients between marbling and IMF content evaluated at the same location varied between 0.51 and 0.66. Prediction of IMF content from CT images is not accurate enough (residual predictive deviation statistical values lower than 1.3). Muscle weight increase with animal growth and allometric coefficients varied between 0.89 and 0.97 for the muscles evaluated. The conclusions of the present work are that IMF content differs between and within muscle, during growth and by genotype-sex and that prediction of IMF in CT images of live pigs is not accurate.