This study aimed to examine and compare the effectiveness of a novel spray-dried inoculant and a commercial freeze-dried additive on the fermentation quality, aerobic stability and bacterial population of vetch-oat silage. An entirely random design used a 3 × 4 factorial arrangement of treatments, with and without lactic acid bacteria (LAB) inoculants and four fermentation periods. Physicochemical parameters, microbiological counts and 16S rRNA gene sequencing analysis on Nanopore MinION were conducted to characterize the ensiling process. Both LAB inoculants increased dry matter, crude protein, lactic, acetic and propionic acid contents, while reducing pH, neutral detergent fibre, ammonia nitrogen/total nitrogen and ethanol concentrations compared to the control group. Overall, the native inoculant decreased the cell load of coliforms, yeasts and moulds. In addition, bio-inoculants enhanced the aerobic stability of vetch-oat intercrops. After ensiling, bacterial alpha diversity decreased noticeably; inoculation reduced the number of observed operational taxonomic units and the Shannon and Simpson indices. Notably, the relative abundance of Lactobacillus in the control group was lower than in treated silages, while the relative values of Staphylococcus increased sharply in the uninoculated group. In conclusion, the native strains showed promise for usage as a bio-inoculant in the ensiling of vetch-oat at a mixture rate of 1:1, producing an immediate impact as well as a favourable effect on the post-opening phase. This represents the first report on 16S rRNA gene-based nanopore metagenomics applied to the bacterial analysis of vetch-oat silage, providing a microbiological insight where native and commercial strains dominate the natural epiphytic community.

Glyphosate is the most widely used herbicide in the United States; however, concern is escalating about increasing residues of glyphosate and its metabolite aminomethylphosphonic acid (AMPA) in soil. There is a lack of scientific literature examining the response of cover crops to soil residues of glyphosate or AMPA. The objectives of this study were to evaluate the impact of glyphosate or AMPA residues in silty clay loam soil on emergence, growth, and biomass of cover crops, including cereal rye, crimson clover, field pea, hairy vetch, and winter wheat, as well as their germination in a 0.07% (0.7 g L–1) solution of AMPA or glyphosate. Greenhouse studies were conducted at the University of Nebraska–Lincoln to determine the dose response of broadleaf and grass cover crops to soil-applied glyphosate or AMPA. The results indicated that soil treated with glyphosate or AMPA up to 105 mg ae kg–1 of soil had no effect on the emergence, growth, above-ground biomass, and root biomass of any of the cover crop species tested. To evaluate the impact of AMPA or glyphosate on the seed germination of cover crop species, seeds were soaked in Petri plates filled with a 0.7 g L–1 solution of AMPA or glyphosate. There was no effect of AMPA on seed germination of any of the cover crop species tested. Seed germination of crimson clover and field pea in a 0.7 g L–1 solution of glyphosate was comparable to the nontreated control; however, the germination of cereal rye, hairy vetch, and winter wheat was reduced by 48%, 75%, and 66%, respectively, compared to the nontreated control. The results suggested that glyphosate or AMPA up to 105 mg ae kg–1 in silt clay loam soil is unlikely to cause any negative effect on the evaluated cover crop species.

Green manure and compost-enriched in phosphorus can promote the sustainability of cropping systems by increasing soil fertility over the long term. They can also be used to manage crop/weed interactions, a key element in guaranteeing an appropriate level of satisfactory crop yields. We studied how green manuring with hairy vetch (Vicia villosa Roth.) and the application of different types of phosphorous-enriched compost affect weed/maize (Zea mays L.) interactions in an organic stockless Mediterranean agroecosystem for two consecutive dry years. Green manure stimulated the expression of maize traits related to a higher competitive ability against weeds, such as early growth, height and leaf area index, while the effect of compost was less clear. Regarding crop/weed competition, both green manuring and a phosphorus-enriched compost application gave a significant advantage to maize. Neither green manure nor compost increased total weed density and biomass compared to the control. Green manuring significantly affected the weed community composition. The relative density of ruderal and competitive-ruderal species (according to Grime's classification) was higher in plots where the green manure was applied. The use of green manure, together with novel composting techniques, significantly affected crop/weed competitive interactions, favoring maize, but also creating favorable conditions for unwanted weed species such as competitive-ruderals. Increasing nitrogen availability in the early growth stages of maize through green manuring can increase crop competitive ability. However, this may not suffice to preserve the system from future weed problems, should potentially detrimental species be selected. Dedicated strategies for the control of emerging weed species may thus be needed.

Cover crops can offer erosion protection as well as soil and environmental quality benefits. Cereal rye (Secale cereale L.) is the most commonly used winter cover crop in corn–soybean rotations in the upper Midwest of the USA because of its superior winter hardiness and growth at cool temperatures. Cereal rye cover crops, however, can occasionally have negative impacts on the yield of a following corn crop, which discourages broader adoption and introduces substantial risk for corn farmers employing cover crops. We hypothesized that because cereal rye shares some pathogens with corn, it may be causing increased disease in corn seedlings planted soon after cereal rye termination. To test this, we performed a series of experiments in a controlled environment chamber to assess the response of corn seedlings with and without a commercial fungicide seed treatment to the presence of cereal rye or other species of cover crops that were terminated with herbicide prior to corn planting. Our results indicate that under cool and wet conditions, cereal rye reduces corn seedling growth performance and increases incidence of corn seedling root disease. Fungicide seed treatment had limited efficacy in preventing these effects, perhaps because environmental conditions were set to be very conducive for disease development. However, hairy vetch (Vicia villosa Roth) and winter canola (Brassica napus L.) cover crops had fewer negative impacts on corn seedlings compared with cereal rye. Thus, to expand the practice of cover cropping before corn, it should become a research priority to develop alternative management practices to reduce the risk of corn seedling root infection following cereal rye cover crops. Over the longer term, testing, selection and breeding efforts should identify potential cover crop species or genotypes that are able to match the winter hardiness, growth at cool temperatures and the conservation and environmental quality benefits of cereal rye, while avoiding the potential for negative impacts on corn seedlings when environmental conditions are suitable for disease development.

Cover crop residues are not widely used for weed control because, as a stand-alone tactic, they do not effectively suppress all weeds and their duration of weed control is too short. Field experiments were conducted in 1995 and 1996, under both irrigated and rainfed conditions, to quantify Amaranthus spp., Setaria spp., and soybean emergence and growth in residues of fall-planted, spring-killed barley, rye, triticale, wheat, and hairy vetch. For both weed species, seedling emergence was reduced 3 wk after soybean planting by rye and wheat residues (≥ 2, 170 kg ha−1) in 1996. In 1996, Amaranthus spp. canopy volume was reduced 38 to 71% by residues 3 wk after planting. Likewise, Setaria spp. canopy biomass was reduced 37 to 97% in residues 5 wk after planting over both years. The response comparison index was used to identify frequency by which weed growth was placed at a disadvantage relative to soybean growth. Amaranthus spp. and Setaria spp. growth suppressions 3 to 5 wk after planting indicate potential times for intervention with other integrated weed management tactics such as reduced postemergence herbicide rates and interrow cultivation.

A small assortment of microbial proteins have the ability to activate defense responses and induce necrosis in plant cells through cell signaling pathways. These proteins are of interest because of their potential use as bioherbicides and inducers of plant resistance in agriculture. A 24-kDa protein (Nep1) was purified from culture filtrates of Fusarium oxysporum, and the effects of this protein on weed leaves were investigated. This protein induced necrosis in detached leaves of Papaver somniferum, Lycopersicon esculentum, Malva neglecta, and Acroptilon repens when taken up through the petiole. The pattern and level of necrosis were dependent on the plant species. Treatment with Nep1 induced the production of ethylene in isolated leaves of various species, and the level of ethylene response was shown to be correlated to the concentration of the protein. Pretreating leaves of P. somniferum, L. esculentum, M. neglecta, and Cardaria draba with 100 µl L−1 ethylene enhanced the protein induction of ethylene biosynthesis in those leaves. Application of Nep1 (200 nM) as a spray to intact plants of Abutilon theophrasti, P. somniferum, Centaurea solstitialis, Centaurea maculosa, and Sonchus oleraceus resulted in extensive necrosis of leaves within 48 h. The results of this research are supplemental to our understanding of the role of specific polypeptides in plant/microbe interactions and demonstrates for the first time that a fungal protein can cause extensive necrosis when applied to weed species as a foliar spray.

Fall-planted cover crops offer many benefits including weed suppressive residues in spring sown crops when controlled and left on the soil surface. However, vegetable growers have been slow to adopt direct-seeding (no-till) into cover crop residues. Field studies were conducted in 2009 and 2010 near Paterson, WA and Urbana, IL to evaluate mortality of rye and common vetch (WA) hairy vetch (IL) cover crops, weed density and biomass, and snap bean growth and yield following four cover crop control methods utilizing a roller–crimper. Rye had higher mortality than common and hairy vetch by roller-crimping, and carfentrazone applied after roller crimping only slightly increased vetch mortality. Heavy residues of rye and escaped vetch were difficult to plant into, often resulting in lower snap bean populations. Rye and hairy vetch residues suppressed final weed biomass, while common vetch reduced weed biomass 1 of 2 years. Escaped plants of both vetch species became a weed. Snap bean yields were inconsistent and often lower following cover crops compared with a fallow treatment. Being able to completely control cover crops and to plant, manage escaped weeds and mechanically harvest in the presence of heavy residues are challenges that deter vegetable growers from readily adopting these systems.

Cover crops play an important role in agricultural sustainability. Unlike commodity cash crops, however, there has been relatively little cover crop breeding research and development. We conducted an online survey to evaluate: (a) the perspectives of organic and conventional farmers in the USA who use cover crops and (b) the specific cover crop traits that are important to farmers. We recruited participants from both organic and conventional agriculture networks and 69% of respondents reported that they farmed organic land. In addition to demographic data and information on management practices, we quantified farmer perspectives on four winter annual cover crops: (1) Austrian winter pea, (2) crimson clover, (3) hairy vetch and (4) cereal rye. Overall, respondents represented a wide range of states, farm sizes, plant hardiness zones and cash crops produced. Of the 417 full responses received, 87% of respondents reported that they used cover crops. The maximum amount farmers were willing to spend on cover crop seed varied by farmer type: 1% of conventional farmers versus 19% of organic farmers were willing to spend over US$185 ha−1 (US$75 acre−1). Organic and conventional farmers differed in terms of the reasons why they grew cover crops, with organic farmers placing greater value on the ecosystem services from cover crops. More organic (63%) than conventional (51%) farmers agreed that participatory breeding was important for cover crop variety development (P = 0.047). Both groups shared strong support for cover crop research and considered many of the same traits to be important for breeding. For the legume cover crops, nitrogen fixation was considered the most important trait, whereas winter hardiness, early vigor, biomass production and weed suppression were the most important traits for cereal rye. Our results illustrate common interests as well as differences in the perspectives between organic and conventional farmers on cover crops and can be used to inform nascent cover crop breeding efforts.

Population genetics of invading pests can be informative for understanding their ecology. In this study, we investigated population genetics of the invasive alfalfa weevil Hypera postica in Fukuoka Prefecture, Japan. We analyzed mitochondrial tRNALeu-COII, nuclear EF-1α gene fragments, and Wolbachia infection in relation to three leguminous host plants: Vicia angustifolia, Vicia villosa, and a new host Astragalus sinicus cultivated as a honey source and green manure crop. A parsimony network generated from mitochondrial gene sequences uncovered two major haplotypic groups, Western and Egyptian. In contrast to reported Wolbachia infection of the Western strain in the United States, none of our analyzed individuals were infected. The absence of Wolbachia may contribute to the stable coexistence of mitochondrial strains through inter-strain reproductive compatibility. Hypera postica genetic variants for the mitochondrial and nuclear genes were associated neither with host plant species nor with two geographic regions (Hisayama and Kama) within Fukuoka. Mitochondrial haplogroups were incongruent with nuclear genetic variants. Genetic diversity at the nuclear locus was the highest for the populations feeding on V. angustifolia. The nuclear data for A. sinicus-feeding populations indicated past sudden population growth and extended Bayesian skyline plot analysis based on the mitochondrial and nuclear data showed that the growth of A. sinicus-feeding population took place within the past 1000 years. These results suggest a shorter history of A. sinicus as a host plant compared with V. angustifolia and a recent rapid growth of H. postica population using the new host A. sinicus.

Organic cropping systems that utilize winter grown cereal–legume cover crop mixtures can increase plant available nitrogen (N) to a subsequent cash crop, but the rate of N release is uncertain due to variations in residue composition and environmental conditions. A study was conducted to evaluate N availability from rye (Secale cereale L.)–hairy vetch (Vicia villosa Roth) cover crop mixtures and to measure the response of organically grown sweet corn (Zea mays L.) to N provided by cover crop mixtures. Nitrogen availability from pure rye, pure hairy vetch, and rye–vetch mixtures was estimated using laboratory incubation with controlled temperature and soil moisture. Sweet corn N response was determined in a 2-year field experiment in western Washington with three cover crop treatments as main plots (50:50 rye–vetch seed mixture planted mid September, planted early October, and none) and four feather meal N rates as subplots (0, 56, 112 and 168 kg available N ha−1). Pure hairy vetch and a 75% rye–25% hairy vetch biomass mixture (R75V25) released similar amounts of N over 70 days in the laboratory incubation. But, the initial release of N from the (R75V25) treatment was nearly 70% lower, which may result in N release that is better timed with crop uptake. Cover crops in the field were dominated by rye and contained 34–76 kg ha−1 total N with C:N ranging from 18 to 27. Although time of planting and management of cover crop quality improved N uptake in sweet corn, cover crops provided only supplemental plant available N in this system.

Novel technologies to reduce tillage in organic systems include a no-tillage roller/crimper for terminating cover crops prior to commercial crop planting. The objective of this experiment was to compare: (1) weed management and yield effects of organic tilled and no-tillage systems for corn (Zea mays L.), soybean [Glycine max (L.) Merr.] and irrigated tomato (Lycopersicon esculentum Mill.), using a roller/crimper and two cover crop combinations [hairy vetch/rye (Vicia villosa Roth/Secale cereale L.) and winter wheat/Austrian winter pea (Triticum vulgare L./Pisum sativum L. ssp. arvense (L.) Poir.)]; and (2) the economic performance of each system. Weed management ranged from fair to excellent in the organic no-tillage system for soybean and tomato crops, with the rye/hairy vetch mulch generally providing the most weed suppression. Corn suffered from low rainfall, competition from weeds and hairy vetch re-growth and, potentially, low soil nitrogen (N) from lack of supplemental fertilization and N immobilization during cover crop decomposition. No-tillage corn yields averaged 5618 and 634 kg ha−1 in 2006 and 2007, respectively, which was 42–92% lower than tilled corn. No-tillage soybeans in 2007 averaged 2793 kg ha−1 compared to 3170 kg ha−1 for tilled soybeans, although no-tillage yields were 48% of tilled yields in the dry year of 2006. Irrigated tomato yields averaged 40 t ha−1 in 2006 and 63 t ha−1 in 2007, with no statistical differences among tillage treatments. Economic analysis for the three crops revealed additional cover crop seed and management costs in the no-tillage system. Average organic corn returns to management were US$1028 and US$2466 ha−1 greater in the tilled system compared to the no-tillage system in 2006 and 2007, respectively, which resulted mainly from the dramatically lower no-tillage yields. No-tillage soybean returns to management were negative in 2006, averaging US$ −14 ha−1, compared to US$742 ha−1 for tilled soybeans. However, in 2007, no-tillage soybean returns averaged US$1096 ha−1. The 2007 no-tillage irrigated tomato returns to management averaged US$53,515 compared to US$55,515 in the tilled system. Overall, the organic no-tillage soybean and irrigated tomato system demonstrated some promise for reducing tillage in organic systems, but until economic benefits from soil carbon enhancement can be included for no-tillage systems, soil improvements probably cannot offset the economic losses in no-tillage systems. Irrigation could improve the performance of the no-tillage system in dry years, especially if grain crops are rotated with a high-value irrigated tomato crop.

Sustainable production systems are needed to maintain soil resources and reduce environmental contamination on erodible lands that are incompatible with tillage-intensive operations. A long-term cropping systems comparison was established at Beltsville, Maryland, on a site with 2 to 15% slope to evaluate the efficacy of sustainable strategies compatible with reduced-tillage systems. All systems followed a 2-year rotation of corn the first year and winter wheat followed by soybean the second year. Treatments included (1) no-tillage system with recommended fertilizer and herbicide inputs, (2) crownvetch living mulch system with similar inputs to the no-tillage system, (3) cover crop system including a hairy vetch cover crop before corn and a wheat cover crop before soybean with reduced fertilizer and herbicide inputs, and (4) manure system including crimson clover green manure plus cow manure for nutrient sources, chisel plow/disk for incorporating manure, and rotary hoe plus cultivation for weed control. Results from the initial 4 years demonstrated the relative productivity of these systems. Corn yields were similar in the no-tillage and cover crop systems in each year; both systems averaged 7.8 Mg ha-1 compared to 5.7 Mg ha-1 in both the crownvetch and manure systems. Wheat yields were highest in the manure system in the first 2 years and in the crownvetch system in the last 2 years. Soybean yields were highest in the cover crop system in all years. The manure system usually had lower yields than the highest yielding systems, partly because of competition from uncontrolled weeds. Several measures of the efficiency of grain production were evaluated. The no-tillage system produced the most grain per total vegetative biomass throughout the rotation. The cover crop system produced the most grain per unit of external nitrogen input and, along with the no-tillage system, had the highest corn water-use efficiency. The cover crop system also recycled the most vegetative residues and nutrients of all systems. No single system performed best according to all measures of comparison, suggesting that trade-offs will be required when choosing production systems.

We conducted a field experiment within a low-input reduced tillage trial to determine how a cover crop affects inoculum levels of vesicular-arbuscular mycorrhizal (VAM) fungi. Plots with and without the hairy vetch cover crop were established on September 30, 1993, under moldboard plow (MP), chisel-disk (CD), and no-till (NT) treatments in low-input (LI) management, and MP in conventional (CONV) management. We conducted a 3-week colonization assay in the greenhouse with bahiagrass seedlings to assess the relative colonization potential of the soils in the fall and following spring. Hairy vetch roots were colonized by indigenous VAM fungi by 65 days after planting, with plants from NT being more colonized than plants from MP or CD plots. Spore populations were greater in the LI than in the CONV system. The beneficial effect of the cover crop on VAM spore populations in soil was manifested in the spring, with the Glomus type group more abundant in plots with cover than without it. The greenhouse bioassay showed that colonization potential of spring 1994 soil samples was higher in plots with cover than without cover for both the LI and CONV systems. Just one season of an overwintering cover crop of hairy vetch increased the inoculum of VAM fungi the following spring before the next cash crop was planted.