Introduction. Rambutan is a tropical fruit species with recalcitrant seeds. Despite the expansion of exotic fruit cultivation in Brazil, lots of which fruit species, including rambutan, need basic information, especially in relation to propagation and storage of seeds, which are important for genetic improvement studies, maintenance of genetic sources and seedling production. Materials and methods. A completely randomized design was adopted with treatments distributed in a factorial arrangement, 3 × 4, referring to three seed storage conditions [room temperature conditions; a dry chamber with (18 ± 2) °C and 60% relative humidity; and a cold chamber with (10 ± 2) °C and 70% relative humidity] and four storage times (0, 7, 14 and 21 d). Each treatment of 10 seeds was replicated five times. Data on seedling emergence, emergence rate, plant height, number of leaves and length of main root were submitted to variance analysis and means were separated using Tukey’s test. Correlation analysis between seed moisture and seedling emergence was performed. Results and discussion. Our results indicated that dry chamber conditions promoted the statistically significantly highest seedling emergence after 7 d of storage. Cold chamber conditions promoted an extremely low seedling emergence independently of time. Conclusion. Rambutan seeds can be stored in a dry chamber for 7 d without losing viability; after 14 d of storage the loss of emergence is 60%.

Introduction. Canarium schweinfurthii, a tree species in the rainforest of Nigeria whose fruits are intensively gathered and eaten, is seriously endangered because of poor seed germinability and usage. Our study examined the pattern of seed distribution of C. schweinfurthii in the soil from the stem base of the parent plant and through the soil profile, and it assessed the seed level of germinability. Materials and methods. Four stands of C. schweinfurthii were selected from a study area in Southeastern Nigeria. Soils were collected from six distances from each trunk base (1 m, 5 m, 10 m, 15 m, 20 m and 25 m) and at four soil depths (0–5 cm, 5–10 cm, 10–20 cm and 20–30 cm); then seeds of C. schweinfurthii were isolated from the cores. Moreover, the percentage of viability and germinability of the seeds collected from each distance and depth were determined. Results. The maximum number of seeds occurred at 1 m from the trunk base (82.3 seeds·30 cm–2), which was 55% of the total collected seeds, and no seed occurred beyond 20 m from the trunk base. The mean number of C. schweinfurthii seeds occurring at 0–5 cm soil depth was 51.3 seeds·30 cm–2, which was the highest from any profile. No seeds occurred below 20 cm. Sixty-seven percent of the total 148 seeds collected were considered viable, with about 80% of them occurring at the 0–5 cm soil depth. Even with the pre-sowing treatment (cutting the hard endocarp), only 30.8% of viable seeds collected from 0–5 cm soil depth were able to germinate, while 10% of those collected from 5–10 cm depth germinated. Conclusion. The localization of over 80% of C. schweinfurthii seeds within a 5-m radius from the stem base and 65% within 0–5 cm soil depth encourages easy fruit gathering by local sellers and leads to depletion of the seed bank. Seedling multiplication, protection of stands and domestication need to be initiated to lift C. schweinfurthii out of endangered status, considering the demand for the fruit and poor seed germination due to the hard endocarp.

Introduction. Cette étude a eu pour objectif d’identifier les principales espèces de mouches des fruits (Diptera, Tephritidae) d’importance économique, inféodées au goyavier dans les vergers fruitiers de la province du Centre, au Cameroun. Matériel et méthode. Ce travail a été réalisé dans trois localités (Yaoundé, Bikok et Essé) de la zone humide du pays. Un total de 270 fruits infestés a été collecté, puis mis en observation jusqu’à émergence des adultes. Résultats. Les fruits collectés ont permis de récolter 1260 puparia à partir desquelles trois espèces de mouches des fruits ont été identifiées : Ceratitis anonae qui représente 64 % des adultes obtenus, Bactrocera invadens (35 %) et B. mesomelas (1 %). Les deux premières espèces, présentes dans les trois localités, sont d’importance économique. B. invadens est une nouvelle espèce invasive pour le Cameroun, tandis que B. mesomelas, non observée à la périphérie de Yaoundé, est nouvellement citée pour ce pays. Conclusion. Ce premier travail d’inventaire devra être suivi d’une étude sur la distribution des espèces identifiées, la gamme de leurs plantes-hôtes, l’évolution de leurs populations et leurs ennemis naturels. Des études complémentaires plus spécifiques devraient permettre d’améliorer les connaissances sur les espèces de mouches de fruits du Cameroun en vue de la mise en place d’une lutte intégrée contre ces importants ravageurs.

Introduction. Saline soils may exert a different effect on seed germination and seedling growth. Materials and methods. The seeds of two rootstocks of pistachio (Pistacia vera), Ghazvini and Badami-e-zarand, were incubated at 20 °C in the dark in a 150 mM NaCl solution or in 150 mM NaCl solutions amended with (50, 100 and 150) mM CaSO4. Seeds were planted in pots containing a mixture of garden soil, sand and compost (1/3 v/v) to investigate the effect of calcium sulphate on plants grown under salt stress. Irrigation water treatments were control (deionised water alone); salinity stress (150 mM NaCl); salinity with 50 mM CaSO4; salinity with 100 mM CaSO4; and salinity with 150 mM CaSO4. Results and discussion. In all treatments, both the final germination percentage and the final percentage of seeds with emerging seedlings longer than 20 mm were higher in the Ghazvini rootstock than in the Badami-e-zarand rootstock. Both the final germination percentage and the final percentage of seeds with emerging seedlings were significantly increased with increasing CaSO4 concentration, except at the highest CaSO4 concentration. The plants grown under 150 mM NaCl produced less dry matter and had lower chlorophyll content than those grown without NaCl. Supplementary CaSO4 only at (50 and 100) mM concentrations ameliorated the negative effects of salinity on plant dry matter and chlorophyll content. Sodium (Na) concentration in plant tissues increased in both leaves and roots of plants under the NaCl treatment alone. The Ghazvini rootstock had much lower Na. Additions of CaSO4 significantly lowered the concentration of Na in both leaves and roots. The Ghazvini rootstock was more tolerant to salinity than the Badami-e-zarand rootstock. The accumulation of Na in leaves and roots indicates a possible mechanism whereby cv. Ghazvini copes with salinity in the rooting medium, and/or may indicate the existence of an inhibition mechanism of Na transport to leaves. Concentrations of Ca and K were lower in the plants grown at high NaCl concentration than in those under the control treatment, and, for the cv. Ghazvini, these two element's concentrations were increased in both leaves and roots for the plants with calcium sulphate treatment; for the cv. Badami-e-zarand, these concentrations were increased in only the roots.

Origin and importance. Acerola, or Malpighia emarginata D.C., is native to the Caribbean islands, Central America and the Amazonian region. More recently, it has been introduced in subtropical areas (Asia, India and South America). The vitamin C produced by acerola is better absorbed by the human organism than synthetic ascorbic acid. Exportation of acerola crops is a potential alternative source of income in agricultural businesses. In Brazil, the commercial farming of acerola is quite recent. Climatic conditions. Acerola is a rustic plant. It can resist temperatures close to 0 °C, but it is well adapted to temperatures around 26 °C with rainfall between (1200 and 1600) mm per year. Fruit characteristics. Acerola fruit is drupaceous, whose form can vary from round to conic. When ripe, it can be red, purple or yellow. The fruit weight varies between (3 and 16) g. Maturation. Acerola fruit presents fast metabolic activity and its maturation occurs rapidly. When commercialised in ambient conditions, it requires fast transportation or the use of refrigerated containers to retard its respiration and metabolism partially. Production and productivity. Flowering and fruiting are typically in cycles associated with rain. Usually, they take place in 25-day cycles, up to 8 times per year. The plant can be propagated by cuttings, grafting or seedlings. Harvest. Fruits produced for markets needs to be harvested at its optimal maturation stage. For distant markets, they need to be packed in boxes and piled up in low layers; transportation should be done in refrigerated trucks in relatively high humid conditions. Biochemical constituents. Acerola is the most important natural source of vitamin C [(1000 to 4500) mg·100–1 g of pulp], but it is also rich in pectin and pectolytic enzymes, carotenoids, plant fibre, vitamin B, thiamin, riboflavin, niacin, proteins and mineral salts. It has also shown active anti-fungal properties. Products and market. Acerola is used in the production of juice, soft drinks, gums and liqueurs. The USA and Europe are great potential markets. In Europe, acerola extracts are used to enrich pear or apple juices. In the USA, they are used in the pharmaceutical industry. Conclusions. The demand for acerola has increased significantly in recent years because of the relevance of vitamin C in human health, coupled with the use of ascorbic acid as an antioxidant in food and feed. Acerola fruit contains other significant components, which are likely to lead to a further increase in its production and trade all over the world.

Introduction. The raphia palm tree is found in abundance in the southern part of Nigeria, especially in the southeastern part. It is grown mainly for its production of palm wine. It also bears fruits (Raphia hookeri) whose pulp is considered edible in some parts of the country and not edible in other parts. Materials and methods. Conventional methods of analysis were used to investigate the vitamin, phytochemical and toxic element composition of the pulp and seed of the fruit. Results. The investigation shows that the pulp has higher concentrations of vitamin E (1.04 mg·100 g–1), niacin (0.2 mg·100 g–1), alkaloid (5 g·kg–1), saponins (3.6 g·kg–1), flavonoids (4 g·kg–1) and phenols (4.1 g·kg–1) than the seed, but the seed has higher values of vitamin A (0.16 mg·100g–1), thiamine (0.07 mg·100 g–1), riboflavin (0.07 mg·100 g–1), nitrates (3.05 mg·100 g–1) and nitrites (0.29 mg·100 g–1), and of the toxic elements: lead (0.03 µg·g–1), mercury (0.04 µg·g–1), arsenic (0.23 µg·g–1) and cadmium (0.04 µg·g–1) than the pulp. Conclusion. The pulp and seed of R. hookeri are non-toxic and can serve as food as well as in medicine.

Introduction. In Nigeria, the species M. barteri is under-exploited although the tree is of both medicinal and nutritional importance. It bears succulent black-purple fruits that are edible and stain the tongue. The seeds are often with a conspicuous caruncle, with the endosperm present or absent. We studied the nutritive values of the fruit and seeds. Materials and methods. After sampling the fruits in a forest of Abia State, Nigeria, they were washed and opened. The seeds were removed from the succulent portion (pulp). Both pulp and seeds were dried in an oven at 65 °C for 5 h, before being ground to obtain powdery samples. The crude protein, moisture, crude fat, crude fiber, ash contents, total carbohydrate, vitamin contents (A, C, E, thiamine, riboflavin and niacin) and mineral elements were determined. Results and discussion. The analyses showed that both the fruit and seed of M. barteri are good sources of essential dietary nutrients. The values obtained are comparably higher than those reported for some common tropical African fruits.

Introduction. The widely recognised dietary and socio-economic value of the shea nut and its butter extract seems to have diminished the usefulness attached to the shea fruit pulp, which is also very important. This is evident from the dearth of scientific information on the nutritional content of the pulp. Materials and methods. We determined the nutritional composition of shea fruit pulp of fruits collected in seven locations spread in the southern Guinea savanna, northern Guinea savanna and the Sudan savanna of Nigeria. Results. Analysis of variance revealed a significant effect of agro-ecological zones on carbohydrate, protein, fibre and energy. The southern Guinea savanna zone recorded comparatively more carbohydrate and energy content than the other two zones, while maintaining lower values for protein and fibre. Conversely, all nutritional traits varied significantly across the fruit pulps for fruits collected in the individual locations surveyed in the three zones. Values for carbohydrate, protein and fat ranged from 29.3–45.3%, 2.6–7.0% and 0.7–1.7%, respectively. Correlation analysis established a positive significant relationship between carbohydrate and energy, but its relationship with fibre and protein was negative and significant. Fibre content maintained a negative relationship with energy while being positively correlated with protein. Principal component analysis identified fibre, energy, ash, protein and moisture as nutritional traits that could be used in classifying shea fruit pulp. Discussion. Our study showed that the Nigerian Vitellaria fruit pulp has adequate nutritional content that is comparable with that of other species. The strong statistical linkage between fibre and protein in shea fruits presents an attractive combination, particularly for children of the rural zones with restricted access to the more conventional and expensive food sources.

Introduction. Mineral status related to micronutrients and sodium is important to obtain high yields, despite the fact that no standardized contents have been established for yellow passion fruit. Biofertilizers have emerged as an important component of the integrated nutrient supply system aiming at an environmentally better nutrient supply because they have a direct action on micronutrient uptake. In this context, the objective of our research was to evaluate the micronutrient and sodium foliar contents of yellow passion fruit plants fertilized with two different biofertilizers in Brazil. Materials and methods. A randomized block design with treatments distributed in a factorial arrangement (2 × 5) referring to two biofertilizers (simple and enriched with Zn, Mg, Mn, Cu, Co, Fe, Ca, B and Mo) and five doses of each biofertilizer [(0.0, 0.6, 1.2, 1.8 and 2.4) L·plant–1] was adopted. Results of boron, copper, iron, manganese, zinc and sodium foliar contents were submitted to variance analysis, the Tukey test and regression analysis. Results and discussion. The results indicate that enriched biofertilizer promoted the statistically highest foliar contents of all elements. The biofertilizer doses applied had different effects on foliar contents of the elements studied. Conclusion. Micronutrient and sodium foliar contents of yellow passion fruit were affected by both the type and doses of biofertilizers. Simple biofertilizer promoted optimum supplies of boron and iron, while enriched biofertilizer supplied boron, iron, manganese and zinc.

The plant. The aguaje (Mauritia flexuosa L.f.) is an Amazonian palm in the process of domestication to be cultivated as a dwarf form. In Peruvian Amazonia, it has a major impact on the economic, social and ecological systems. Fruit are sold and take part in the economy of Peruvian Amazonia. The insects. In this survey, the authors present 18 species of insect pests observed in eight different localities in the surroundings of the city of Iquitos (Loreto, Peru) between 2000 and 2003. Among those species, four develop in the pulp of the fruit, two are borers of the rachillae, one is a borer of the trunk and the petioles of the young leaves, two species bore the rachillae and floral buds, one develops in buds of female flowers and another damages the base of the roots; eight of those species are considered as the most important pests: Eupalamides cyparissias Fab., Antiteuches kerzhneri Rider, Stenoma sp., Clinodiplosis sp., an unidentified species of Cecidomyiidae, Tyrannion sp. and two unidentified species of Curculionidae. Discussion. The cultivation of the dwarf aguaje is a good alternative to the cutting of the native palms to harvest the fruit, but the cultivated palms are damaged by insects. The planters must learn to know those pests to control them. The biology and the ecology of the new pests are currently being studied.

Introduction. Prunus amygdalus and P. webbii can be used as rootstocks for almond cultivars due to their adaptability to severe environmental conditions. However, the time required for seedlings to reach a suitable size for transplanting may take 1 to 3 years. Our study tested the effects of foliar application of growth regulators on the vegetative growth and carbohydrate accumulation in shoots and roots of these species. Materials and methods. Six-week-old seedlings were treated with gibberellic acid (GA3) (100 mg·L–1) alone or with GA3 followed by ethephon [(100 and 200) mg·L–1], or chlormequat chloride (CCC) [(500 and 1000) mg·L–1], or paclobutrazol (PBZ) [(500 and 1000) mg·L–1]. Results and discussion. Most levels of plant growth regulators significantly enhanced seedling growth. However, GA3 alone was most effective on stem height, leaf area, and shoot fresh and dry weights of both almond species. The thickest stems of P. amygdalus and P. webbii were obtained from the application of 100 mg GA3·L–1 followed by application of (1000 and 500) mg PBZ·L–1, respectively. In both species, PBZ significantly increased leaf chlorophyll content compared with the controls as well as with the other treatments. Application of GA3 alone on P. webbii and of GA3 followed by 100 mg ethephon·L–1 on P. amygdalus showed the highest root number, and root fresh and dry weights. High levels of soluble sugars and starch in the shoots and roots of both species were observed when GA3 application was followed by PBZ. Conclusion. These results demonstrated that application of plant growth regulators to the seedlings might be a useful way of enhancing growth of P. amygdalus and P. webbii and reducing the time and cost of seedling production.

Introduction. This protocol aims at evaluating (a) the efficacy of new fungicides for the control of post-harvest diseases, (b) the efficacy of various application methods for the chemical control of post-harvest diseases, and (c) the quality of the fungicide solution during the same packing day where this solution is recycled. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. Materials required and details of the eighteen steps of the protocol (fruit sampling and inoculum preparation, wound anthracnose study, quiescent anthracnose study, and crown-rot study) are described. Results. Comparison between untreated control bananas and bananas treated with fungicide allows the calculation of the fungicide treatment efficacy.

Introduction. The production of high quality fruit juice involves several cultural inputs. Among other factors, fruit quality attributes are influenced by the cultivar, the climate, the harvesting time and soil fertility. Particularly, the soil fertility has a significant effect on the nutritional characteristics of the juice. Good practice as regards fertilization consists of applying rates adapted to plant optimum growth, yield and fruit quality. Materials and methods. Effects of four poultry manure rates [(0, 5, 10 and 15) t·ha–1] on the juice quality of passion fruits (Passiflora edulis var. flavicarpa) were evaluated for two cropping years (2005 and 2006). The seedlings of passion fruit vine were field-established in a randomized complete block design, and the manure treatments were replicated four times. Juice quality assessment was performed on fruits picked in December 2005, coinciding with the dry season and low soil moisture recharge, and August 2006, during the wet season and high soil moisture recharge. Results. The results obtained indicated a significant poultry manure effect on all the juice quality parameters studied; the quality of the juice increased gradually as the manure rate increased. Similarly, the season of fruit-picking had a significant effect on the juice quality. As expected, vines that received no manure produced fruits with the poorest juice quality, suggesting unfavorable nutritive conditions within the vines. The concentrations of anti-nutrient factors (tannin, hydrogen cyanide, phytate and calcium oxalate) were low in ripe yellow passion fruits, and insignificant regarding health hazards for consumers of yellow passion fruit. Conclusion. The results obtained showed that the manure rate and the harvest period affected the quality of passion fruit juice. Application of 15 t·ha–1 poultry manure generally gave the best juice quality; similarly, fruits harvested in the first cropping season had better juice quality.

Introduction. The fruit of the date palm (Phoenix dactylifera L.) is an important crop of the arid and semi-arid areas of the world. It has always played a genuine economic and social part in the lives of the people of these areas. Nevertheless, there are few studies related to the composition and the functional properties of the different components (fibres, fats and proteins) of the date seeds. In order to obtain more information on the nutritive value and the chemical composition of the pits of the most important and most valuable Tunisian date palm varieties (Deglet Nour and Allig), we studied their profiles of proteins and amino acids. Materials and methods. Date seeds of two Tunisian cultivars, Deglet Nour and Allig, were separately milled in a heavy-duty grinder to pass 1–2 mm screens; then, they were preserved at – 20 °C until analysis. The soluble proteins from defatted date seeds were extracted with water at pH 10, and were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Results and discussion. Amino acid profiles revealed that date seeds from the Deglet Nour and Allig varieties contained the majority of essential amino acids: lysine, isoleucine, leucine, methionine, threonine, valine and phenylalanine. Seventeen amino acids were detected in the seeds of the two studied cultivars. Glutamic acid was the major amino acid in Deglet Nour and Allig date seeds, representing 17.83% and 16.77%, respectively, of the total amino acids. Date seeds appeared to contain a number of proteins with molecular weights ranging from 22 kDa to 70 kDa. Three prominent bands appeared at 32 kDa, 60 kDa and 70 kDa.

Introduction. Under the sub-tropical climate of India, ‘Sweet Charlie’ strawberry is being grown on a large scale. However, under such climatic conditions, it is not possible to transplant runners before mid-October, because the environmental temperature is very high, and planting after mid-October is also not good because then winter sets in and affects growth, development, flowering and fruiting of strawberry. Under such circumstances, covering of strawberry beds with row covers and mulching with plastic mulch can be useful for early and high production by way of modifying the leaf physiology and producing marketable berries in high number. Materials and methods. Row covers of transparent polyethylene (100-µm thickness) were erected over beds after one month of transplanting of the runners. Mulching (black polyethylene, white polyethylene and paddy straw) was also applied after one month of transplanting of the runners; it was removed only after fruiting was over. Observations on different leaf physiological parameters were made. Results. Row covers and mulching individually or in combination significantly influenced plant physiology, growth, berry yield attributes and albinism incidence. Plants showed better growth and physiological attributes under row covers than without row covers. Plants had the best growth and physiological attributes under black polyethylene mulch. Berry weight and yield were the highest in plants under row covers and black polyethylene mulch. Although the [row covers × mulching] interaction showed the highest incidence of albinism, berry yield per plant (excluding albino fruit) was significantly the highest with that combination. Conclusion. Erection of row covers and mulching in winter is highly useful for better growth and production of strawberry under a sub-tropical climate.

Introduction. In subtropical regions, the lack of or insufficient winter chilling has been one of the most important yield-limiting factors for table grapes. Winter chilling for several weeks is required to cause the transition of both vegetative and floral buds from the dormant to the active state. Thus, the effect of time of application of Dormex® (hydrogen cyanamide) on fruit quality and ripening of ‘Superior’ seedless grape was evaluated under Jordan Valley conditions.Materials and methods. Five-year-old ‘Superior’ seedless vines were pruned on 20 December and sprayed to run-off with a 5% (v/v) concentration of Dormex® at pruning (Dormexprun0), 7 d (Dormexprun7) or 14 d (Dormexprun14) after pruning in comparison with the pruned-only control plants. The date of vegetative budbreak and start of flowering were determined. Fruits were harvested at intervals of 7 d starting from 9 May, with four harvests. Studied data were fruit weight, total soluble solids (TSS), titratable acidity (TA) and maturity with the [TSS:TA] ratio. Results and discussion. All pruned and sprayed vines broke bud 23–46 d after pruning. The (Dormexprun0) and (Dormexprun7) treatments were the earliest. Compared with the control, Dormex®-treated vines broke bud 4–26 d earlier. All pruned and Dormex®-treated vines started flowering 55–64 d after pruning, with 4–13 d earlier than the control. Fruits of the (Dormexprun0) treatment had significantly the highest fruit weight, TSS and the least TA, and it was the only treatment that exceeded the [18:1] (TSS:TA) level. Additionally, higher TSS and lower TA were observed for fruits of Dormex®-treated vines during all harvest dates in comparison with the control. Conclusion. Our study showed that, under southern Jordan Valley conditions, Dormex® should be applied as early as at pruning time to obtain early budbreak and maturity.

Introduction. This protocol makes it possible to determine the chromosome number of banana clones in root tip squashes. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. This part describes the required laboratory materials, and the four steps of the protocol used for the chromosome counts (root sampling and treatment, slide preparation, observation of useful cells and chromosome counts on useful cells). Possible troubleshooting is discussed. Results. The protocol usually results in condensed and intensely stained chromosomes.

Introduction. The centrifugal-flotation technique allows all nematode species and life stages to be separated from root debris and residual soil particles to facilitate their observation. The principle of the method applied, key advantages, starting plant material and time required are presented. Materials and methods. Necessary laboratory materials, and details of the 19 steps required for extracting nematodes from plant tissue for counting are described. Possible troubleshooting is explained.

Introduction. This rearing technique is applicable to migratory nematodes (e.g., Pratylenchus spp. and Radopholus similis) for mass production of nematodes for experimental purposes, conservation of nematodes, and direct studies of nematodes’ multiplication or reproduction mechanisms. The principle of the method applied, key advantages, starting plant material and time required are presented. Materials and methods. Necessary laboratory materials, and details of the nine steps required for the preparation of carrot discs, preparation of nematodes, inoculation of nematodes and collection of nematodes out of petri dishes are described. Possible troubleshooting is explained. Results. Several thousands of nematodes can be extracted out of one carrot disc after 6–8 weeks of culture, depending on nematode species and geographical population.

Introduction. This protocol describes a method for obtaining in vitro germination of zygotic embryos from open-pollinated wild seedy bananas and controlled hybridizations. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. This part describes the required laboratory materials, medium preparation, embryo extraction and culture. Results. Embryos typically begin germination in the dark about 15 days after inoculation on germinating medium. After 30––40 days of culture, plantlets are subcultured on a plant growth medium and placed in the light to achieve plant growth and rooting. The ratios of zygotic embryos obtained vary with the types of crosses achieved between Musa sp. genotypes.