In recent years there has been considerable genetic progress in milk production. Yet, increases in yield have been accompanied by an apparent lengthening of calving intervals, days open, days to first heat and a decline in conception rates, which appears to be both at the genetic and phenotypic level. Fertility has a high relative economic value compared to production traits such as protein, making it attractive to include in a breeding programme. To do this there needs to be genetic variance in fertility. Measures of fertility calculated from service dates have a small genetic compared to phenotypic variance, hence heritability estimates are small, typically less than 5%, although coefficients of genetic variance are comparable to those of production traits. Heritabilities of commencement of luteal activity determined using progesterone profiles are generally higher, and have been reported as being from 0.16 to 0.28, which could be because of a more precise quantification of genetic variance, as management influences such as delaying insemination and heat detection rates are excluded. However, it might not be the use of progesterone profiles alone, as days to first heat observed by farm staff has a heritability of 0.15. The most efficient way to breed for improved fertility is to construct a selection index using the genetic and phenotypic parameter estimates of all traits of interest in addition to their respective economic values. Index traits for fertility could include measures such as calving interval, days open, days to first service, or days to first heat but there may also be alternative measures. Examples include traits related to energy balance, such as live weight and condition score (change), both of which have higher heritabilities than fertility measures and have genetic correlations of sufficient magnitude to make genetic progress by using them feasible. To redress the balance between fertility and production, some countries already publish genetic evaluations of fertility including: Denmark, Finland, France, Germany, Israel, The Netherlands, Norway and Sweden.

Increased genetic potential for milk production has been associated with a decline in fertility of lactating cows. Following parturition the nutritional requirements increase rapidly with milk production and result in negative energy balance (NEBAL). NEBAL delays the time of first ovulation thereby affecting ovarian cycles before and during the subsequent breeding period The effects of NEBAL on reinitiation of ovulation are manifested through inhibition of LH pulse frequency and low levels of glucose, insulin and IGF-I in blood that collectively restrain oestrogen production by dominant follicles. Upregulation of LH pulses and peripheral IGF-I in association with the NEBAL nadir increases the likelihood that emerging dominant follicles will ovulate. The legacy of NEBAL is reduced fertility after insemination in conjunction with reduced serum progesterone concentrations. Diets high in crude protein support high milk yield, but may be detrimental to reproductive performance. Depending upon protein quantity and composition, serum concentrations of progesterone may be lower and the uterine luminal environment is altered. High protein intake is correlated with plasma urea concentrations that are inversely related to uterine pH and fertility. The direct effects of high dietary protein and plasma urea on embryo quality and development in cattle are inconsistent. In conclusion, the poor fertility of high producing dairy cows reflects the combined effects of a uterine environment that is dependent on progesterone, but has been rendered suboptimal for embryo development by antecedent effects of negative energy balance and may be further compromised by the effects of urea resultingfrom intake of high dietary protein.

Antinutritional factors in livestock foods may be defined as the substances which either by themselves, or through their metabolic products arising in the system, interfere with food utilization and affect the health and production of animals. These deleterious substances, also referred to as anti-quality factors can be divided tentatively into four groups based on their effects on livestock: (i) affecting protein utilization and depressing digestion (protease inhibitors, tannins, saponins, lectins, etc.), (ii) metal ion scavengers (oxalates, phytates, gossypol pigments, glucosinolates), (Hi) antivitamins, and (iv) those which cannot be put in the above categories (mycotoxins, mimosine, cyanogens, nitrates, alkaloids, photosensitizing agents, isoflavones, etc.). This paper discusses only those deleterious substances which are of prime concern to the farmers and scientists of developing countries. Much emphasis is given to polyphenols compounds, as this class of anti-nutritional factor is the most widespread in non-conventional foodstuffs, i.e. agroindustrial and forestry byproducts. The effective utilization of these non-conventional foods forms the main area of research in the third world countries due to shortages of conventional foods. Efforts have been made to present the current knowledge on their assay methods, effects on animals, mode of action and detoxification/detannification using physical, chemical and biotechnological approaches. Other anti-quality factors viz., mycotoxins, mimosine, cyanogens and nitrates which also cause damage to livestock in developing countries are also discussed briefly.

Whittemore and Fawcett (1976) and Whittemore (1976) showed how ideas and data from different disciplines could be brought together to simulate, using a computer program, the growth of the pig on controlled feeding. Controlled feeding, as shown in Figure 3.3.1, is one of three types of feeding system (Emmans, 1981). Parks (1970) dealt with systems of the second type, in which animals are given free access to single feed. In such a system, the composition of the diet, but not its amount, is known in advance. Parks (1970) suggested that the rate of feed intake in growing animals increased at a diminishing rate with age towards an asymptotic value; Figure 3.3.2. This function has two parameters — the asymptote and a time constant. The values of these were seen as functions of feed composition, genotype and environment. He went on to predict growth as a function of feed intake and a conversion efficiency. While a description of how ad libitum feed intake changes with age is useful, it is not as powerful as an understanding of what governs feed intake would be. While there are many theories about the mechanisms that animals use to control their feed intake at a given level, there is none that can be used to predict what this level will be. One is proposed in this paper.

An analysis on the outcome of 124 inseminations monitored using daily milk progesterone concentration data revealed that those cows in which pregnancy did not occur experienced a 1.7day delay in post-ovulatory luteal progesterone rise when compared to the pregnant group (P<0.001). This suggests that luteal progesterone secretion needs to be initiated prior to day 5 post-ovulation. A comparison between the milk progesterone concentration on day 5 post-ovulation and the pregnancy rate for 1451 inseminations showed the maximum pregnancy rate to occur when the milk progesterone concentration was between 3-9ng/ml. Pregnancy rate fell significantly (P<0.01) when progesterone concentrations were below 3ng/ml, identifying cows that may benefit from progesterone supplementation. The supplementation of progesterone in cows with a day 5 milk progesterone concentration of less than 3ng/ml, using an intra-vaginal progesterone releasing device, only provided benefit for those cows with a day 5 progesterone concentration of between 1-2ng/ml, resulting in a doubling of the pregnancy rate. This data shows that a delay in post-ovulatory progesterone secretion has a detrimental effect on pregnancy but that not all cows with a deficient progesterone concentration benefit from progesterone supplementation.

Genetic progress in poultry species for meat production has contributed to the consistent growth in world production of poultry meat. The poultry species have a number of advantages over the larger species used for meat production. It is possible to maintain large pedigreed populations and use their high reproductive rates to transfer genetic progress to the production generations in less than five years. These populations continue to maintain high heritabilities despite, in some cases, prolonged selection. The history of selection progress in broiler chickens (Gallus gallus domesticus) is reviewed and compared with rates of progress in the duck (Anas platyrhyncos) and the turkey (Meleagris gallopavo).

The rates of genetic change for production traits such as growth, feed efficiency and yield have changed the physiology of the birds. Changes in selection criteria have been made to improve the robustness of the production stock. This allows them to perform well in a wider range of environments. These have been combined with improved definitions of the optimum environments for the birds to minimise any impact on welfare and health. This paper describes examples of selection in the broiler chicken aimed at improving skeletal quality and resistance to ascites. A number of the factors influencing future selection criteria are discussed. Breeding programmes have adapted to respond quickly to adverse genetic correlated responses. The need to combine selection for a large number of traits requires that the programmes are very efficient and use the best statistical techniques available for multivariate breeding value estimation.

The financial implications of delays in conception at different stages of lactation in the average and the high yielding dairy cows were investigated. Variables included in the calculations were net loss in annual yield, lost income from a calf, cost of extended dry period, cost of slipping in calving pattern, cost of extra veterinary treatments and AI services, benefit of a delay in calving for reduced risk of production diseases, potential benefit of delay in conception on milk yield from the current lactation, and the value of quota leasing. For the average yielding cow, the net cost of one day of delay in conception was calculated at £2.41 when conception is delayed from 85 to 100 days post-calving, increasing to £5.02 per day if conception occurred at 146 to 175 days post-calving. After taking value of quota leasing into account, the net cost of a lost day was calculated at £1.73 and £3.55 per day for the two delay intervals respectively. For the high yielding cow, the net cost of one day of delay in conception was calculated at £2.48 when conception is delayed from 85 to 115 days post-calving, increasing to £6.52 per day if conception occurred between 206 and 235 days post-calving. The net costs after quota leasing being considered were calculated at £1.68 and £4.08 per day for the two delay intervals respectively. On the basis of the above estimations, and after considering the cost of culling for poor fertility, it was concluded that it is a cost-effective option to keep trying to get the typical average cow in calf until 266 days post-calving, whereas the breakeven point for the high yielding cow is at 290 days post-calving.

Over the past 30-40 years genetic improvement and better nutrition of dairy cows have led to a significant increase in milk production per cow but this is associated with an increase in cow reproductive wastage. Reproductive wastage in the dairy herd particularly in a seasonal calving system, results in a serious financial loss. Early embryo death accounts for a significant portion of cow reproductive wastage and information is becoming available on the extent and timing of early embryo loss and on aspects of embryo growth, development, metabolism and viability. Such information is necessary to facilitate objective investigation of factors that contribute to early embryo death. For heifers and moderate yielding dairy cows published estimates of fertilisation rate of about 90%, and of average calving rates of about 55% indicate an embryonic and foetal mortality rate of about 40%. Of this total loss, 70 to 80% is sustained between days 8 and 16 after insemination, a further 10% between days 16 and 42 and a further 5-8% between day 42 and term. In high yielding cows there is some evidence of a higher increment of late embryo loss. During the period of greatest embryo loss, between days 8 and 16 after fertilisation, there is a dramatic increase in the growth rate and protein content of embryos, particularly from day 13 to day 16 when the increase is exponential. There is evidence that from day 13 to 15 cattle embryos undergo time and developmental stage-dependent changes in the rate of de-novo protein synthesis and protein phosphorylation. It seems that by the time cattle embryos have elongated they have passed their maximal synthetic activity in terms of protein synthesis and phosphorylation, which seems to occur at day 13 or earlier. While there is little published information on the causes of embryo loss it is clear that even a short-term reduction in energy intake near the time of insemination can significantly reduce embryo survival rate. Abo and low post-ovulatory systemic progesterone has been associated with increased embryo loss. Recent evidence shows that elevated systemic concentrations of ammonia and urea per se do not reduce embryo survival rate but there may be other modifying factors, such as negative energy balance, operating in the high yielding dairy cow that lead to reduced fertility when the systemic concentrations of urea and or, ammonia are high.

Breeding programmes and improved knowledge of genetics have resulted in gradually increased genetic changes in pigs. In this paper the genetic changes in the European pig during the last century are analysed and compared with societal needs for the following decades. Dutch data are used to support the general trend in Europe and results of an EC project on breeding and society are used to identify societal needs.

Genetic changes in pigs started early this century, mainly in Northern Europe, by the gradual set up of local and regional breeding programmes. In the first 60 years the goal of these programmes was focussed only on leanness and feed efficiency. However, type, leg quality and breed characteristics were of utmost importance in these years for some breeding programmes. During these decades the genetic changes were negligible for reproduction traits. Progress was achieved especially for breed characteristics like type and colour and later also backfat thickness, daily gain and consequently improved feed efficiency.

With the introduction of crosses in the early 60's, specialisation in dam and sire lines but moreover in breeding programmes arose in the pig industry. This specialisation resulted rapidly in increased genetic changes, more breeds and/or lines and breeding goals that supported efficient lean meat production systems. Especially in the sire lines, this specialisation resulted in increased progress for daily gain, backfat thickness and feed efficiency. Improved knowledge of genetics increased genetic changes in leanness and feed efficiency during the last few decades, but at the same time attention could be and was paid to fertility and vitality. During the last decade most European pig breeding programmes realised anual genetic progress for daily gain of +20 g/day, lean meat % of +0.5% and litter size of +0.2 piglet/litter. Molecular genetics does not enable more genetic change for these traits, but does so especially for expensive or difficult to measure traits, e.g. meat quality, health and longevity. In commercial pig breeding programmes selection limits have not been reached yet. Nevertheless, there are experimental results that indicate unintended side effects of the present breeding goals.

Next to changes in breeding possibilities, the societal needs have also changed. During the first 80–90 years of the century, pig producers were aiming at production of lean meat at low(er) prices. In this period low prices were more important than quality and/or welfare of the pigs. Nowadays, diversification in consumers' needs is growing and quality of the product (including production circumstances) is important in Western Europe. The market for pork is changing from a market of producers into a market of (potential) buyers. Clearly, the success of any pig breeding programme next century will be judged by the consumer. Where a newly bred, or genetically modified, variety of animal fails to provide something people are prepared to buy, the breeding programme will be judged a failure. In this respect ethical issues are also becoming more important. Modern breeding techniques can threaten animal welfare; a very fast increase in one or a few traits can easily result in an insufficient increase or adaptation of the organs and skeleton. Animal integrety is another important issue, especially related to genetically modified animals. Clearly, the relationship between animal and human interests is central to any view.

From the analysis of genetic changes in pigs and the present and foreseen changes in market demands, it may be concluded that the pig breeding programmes were very successful; but they will have to change their goals. Less emphasis will be needed on low cost price and more emphasis on product quality, biodiversity and well balanced genetic changes for economically important traits.

During recent decades, the UK dairy industry has had to adjust to the introduction of milk quotas in 1984, the deregulation of milk markets in 1994, and accommodate changes in the demand for dairy products. The combination of these factors, in addition to Bovine Spongiform Encephalopathy and Foot and Mouth disease, and a fall in milk price has inevitably resulted in a restructuring of the industry, but also reinforced the need for all sectors of the industry to respond to the prevailing economic climate and changes in consumer preferences.

Breeders of poultry and pigs have selected for some combination of increased growth rate, decreased fatness and increased muscularity. Increasingly various fitness traits are included in the index used. The consequences of such selection include complex effects on nutritional and environmental requirements, at least some of which are reliably predictable using suitable models. Appropriate changes to the environment and to nutrition as selection proceeds will help to avoid unwanted effects occurring. Among the predictable effects are that higher ratios of nutrients to energy, and lower temperatures, will be needed by the improved genotypes. Selection for growth rate must eventually exhaust the capacity of the support systems – digestive, respiratory, circulatory and excretory – to cope with the increased metabolic rate. Selection for increased yield of valuable parts will cause these problems to occur earlier. While it is possible to predict that these problems will occur it cannot be predicted when they will. Breeders need to be aware of these problems, and use all possible routes to help them in reducing their severity. Where the appropriate actions for fitness selection, and nutritional and environmental modifications, are taken the occurrence of the problems will be delayed.

Genotype by environment interaction was investigated for a range of traits. Selection (S) and control line (C) cows, housed and managed at the Langhill Dairy Cattle Research Centre, were offered ad libitum complete mixed diets, with proportions (in total dry matter) of concentrates, silage, brewers’ grains of either 20:5:75 (LC; 1.0 t concentrate per annum) or 45 :5 :50 (HC; 2.5 t concentrate), over a full lactation. No diet X genetic line interactions were observed for a range of traits (production, food intake, live weight and condition score) when compared as means. However, regression coefficients of protein yield (P < 0.05), milk yield (P < 0.10) and protein percentage (P < 0.10) on pedigree index for fat plus protein yield were different between LC and HC. Genetic correlations between HC and LC were high for most traits, and only for fat yield was the value below 0-80. Because the rate of increase in energy output from selection is unlikely to be matched by the rate of increase in intake during the first part of the lactation, it was expected that intake capacity is likely to become more important in the future, regardless of the feeding system. For this reason, it is suggested that selecting for a lower live weight whilst simultaneously selecting for increased yield (an approach which has been advocated in some countries) should be treated cautiously. Especially on low input diets increases in yield as a result of continued selection (on higher input diets) may be limited by the rate, or extent of tissue mobilization; under these circumstances health and fertility problems might be the first indicators of such limits being approached.

A number of measurable physiological events characteristically occur and cause changes during the perioestrual period including the classical, diagnostic sign of standing behaviour. The onset of oestrus coincides with peak titres of oestradiol-17β that subsequently induce the preovulatory surge of LH within 1 to 3 h and ovulation of a mature follicle some 24 to 32 h after the onset of oestrus. Although detection efficiencies are consistently greater in higher producing herds, oestrus-detection efficiency generally has declined in recent years as herd size and milk production have increased. New technologies have introduced some needed assistance for detecting cows in oestrus. These include various in expensive heat mount detectors to more sophisticated electronic gadgetry, such aspedometry and radiotelemetric sensors that detect temperature, tissue impedance, and pressure. Oestrus detection aids are usually more efficient but not necessarily more accurate than visual observation. Differences in housing and environmental conditions, in addition to labor inputs, costs, and efficacies, result in variable acceptance of such technologies. Detection efficiency and accuracy can be improved by simultaneous use of synergistic technologies; those that compliment each other and monitor different indicators of oestrus. Combining technologies for simultaneous measurements of several physiological events associated specifically with the onset of oestrus and their radiotelemetrically signaling to a central computer for subsequent analysis should provide greater efficiency ofoestrus detection with fewer false positives. The ultimate goal of determining the onset of oestrus or ovulation is to predict the optimal timing for insemination. Ultimately, herd personnel must interpret information gathered by these technologies and judge whether or not and when to inseminate cows based on their visual inspection of identified cows.

1. It is shown how the growth of an animal in non-limiting conditions can be predicted from the values of a small set of animal variables. The functions used are derived from a small set of simple, and reasonable, assumptions.

2. Growth may be limited by intakes of nutrients and energy which are less than those needed. The prediction of growth where such limitation occurs calls for the prediction of the intakes. There may be genetic variation in the abilities of animals to deal with the relevant constraints which are, commonly, feed bulk or hot environments.

3. Breeders need to pay more attention to giving sufficient descriptions of their stocks.

The strategy most widely adopted to improve milk production efficiency is to increase yield per cow. To date, this has been achieved primarily through genetic selection and improved nutrition. Achievement of very high individual yield has had its down-side, especially in terms of reduced reproductive efficiency and there is now quite widespread concern that the high genetic merit cow is at greater risk of metabolic disease than her unimproved counterpart. To quote from the recent Farm Animal Welfare Council Report on Dairy Cow Welfare (FAWC, 1997): ‘High metabolic turnover in cows can be associated with a greater risk of mastitis, lameness, infertility and other production diseases…’. Whilst there can be little doubt that metabolic turn-over is indeed higher in high merit cows, it is not safe to assume that this necessarily equates with more risk; metabolic turn-over is higher in an elephant than in a mouse but risk is certainly not. Metabolic load might be a better term to use. If we think, simplistically, of this being the ‘strain’ on a system it is logical to expect an inverse relationship between metabolic load and health. The extrapolation to high genetic merit cows being at greater risk then presupposes that they experience an increased metabolic load but there has been no rigorous evaluation of whether this is so. In this review we will consider what is meant by metabolic load, examine in qualitative theoretical terms what degree of load might be expected from different commercial systems and present some recently obtained data which addresses directly the question, is metabolic load greater in high genetic merit cows?

The relations between genetic change in domestic livestock and infectious disease (including both its epidemiology and the animal's reaction to it) are examined. The overall picture is confusing because there are different, and seemingly unrelated, ways of considering the issue. An attempt is made to put these together into a more general framework. Four processes of particular interest are distinguished and discussed in more detail: (i) the way a population's genetic potential for immunocompetence can be changed by breeding, (ii) the way an animal's immunocompetence is influenced by that animal's production potential, in combination with the environmental resources that are available to it at a given time, (iii) the way the disease status of an animal (and a population of animals) is influenced by its immunocompetence, and (iv) the way the production level of an animal is influenced by activation of its immune system. Ultimately, all four processes influence the realised level of production.

This comes down to four questions that need to be addressed: (i) can we use genetic variation in immunocompetence in animal breeding? (ii) does a higher production potential (today's direction of breeding) have a negative impact on immunocompetence? (iii) does improved immunocompetence result in improved health? and (iv) how large is the negative impact of disease on production?

The high yielding dairy cow is expected to produce a substantial milk output every year and at the same time to conceive and maintain a pregnancy to term. To fulfil lifetime production potential a balance between yield, fertility and other influential factors has to be achieved. Any inability on the part of the management system to identify and rectify problems or on the part of the cow to cope with metabolic demands invariably results in economic or welfare issues. Our studies of high yielding dairy cows have revealed that some animals are capable of normal reproductive function whilst others are classic repeat breeders (requiring 3+ services per conception) or simply fail to rebreed. It is well established that the somatotrophic axis (growth hormone and insulin-like growth factors) drives lactation in ruminants but it is also intimately involved in reproductive processes. An awareness of metabolic adaptations to lactation that impact on reproduction in dairy cows is needed for appropriate management.

The objective of our studies was to explore the metabolic profiles of high yielding dairy cows to identify factors influencing their ovarian function and fertility, hence to characterise the physiological adaptations involved. Our studies revealed different relationships between progesterone profile categories and metabolic status post partum. Delayed ovulation (DOV) or persistent corpora lutea (PCL) may be an appropriate response to a nutritional state or physiological situation and it may therefore be inaccurate to refer to these as ‘abnormal’. Whilst associated with high milk yields, not all profile categories detrimentally affected fertility parameters. Delayed ovulation postcalving (DOV1) was identified as the most prevalent abnormal profile encountered in first lactation high yielding cows. This may have occurred because the cows were not yet physically mature and unable to sustain both milk production and growth. The condition lasted long enough (71 ± 8.3 days from calving) to have a detrimental impact on their overall fertility parameters and was associated with significant physiological changes, representative of tissue mobilisation. Although the incidence of persistent luteal phases (PCL1 and PCL2) in dairy cows is increasing, this condition was not found to have any substantial detrimental effects on fertility or production parameters of the primiparous or multiparous cows in these studies. The main reproductive problems in our high yielding primiparous and multiparous cows appeared to be a failure to ovulate and conceive at the expected time or to maintain a pregnancy. These situations were associated predominantly with high milk yields and low concentrations of plasma IGF-I. A failure to ovulate appears to occur when body reserves are mobilised to maintain milk yield at the expense of reproduction and seems most likely to occur in primiparous high yielding cows or those experiencing GH-resistance (low IGF-I) due to excessive body condition loss, reduced feed intakes and factors such as stress and disease. More detailed investigations of dietary means of increasing IGF-I and optimising insulin concentrations, targeted at important reproductive times, are required in high yielding dairy cows, to aid in their management.

To assess farm animal welfare we need to understand how animals make choices and how these choices relate to preference strength. Studies of environmental choice can be categorized by the method used to investigate them, or by the underlying basis on which the animal is choosing. Choices made between resources that vary along a single dimension should meet certain criteria e.g. those of transitivity. Choices made between resources that vary along more than one dimension may or may not meet these criteria, depending how the animal evaluates each option. Understanding how farm animals choose will allow the results of individual experiments to be applied in a wider context. It is also important to know how preferences are formed during development. Evidence suggests that preferences for nests and pecking substrates in hens may be influenced by prefunctional experience. Experimental data from studies of environmental choice may enable us either to provide important resources in commercial systems, or to provide facilities for animals to continue to make their own decisions.

Selection of dairy cattle for increased milk production has decreased some indices of reproductive efficiency. For example, days open are increased by one day for every 100 kg of increased milk yield per lactation. Some of the change in days open can be explained by delayed onset of oestrous cyclicity and lower conception rate to artificial insemination in cows with greater milk production. Despite these negative associations between milk production and reproduction, reproduction in herds of high producing dairy cattle is not necessarily compromised relative to reproduction in herds of low producing dairy cattle. This is because there is a large environmental effect on dairy reproduction. High producing herds generally have better management and better oestrous detection. Therefore, high producing dairy herds may partially overcome the antagonistic relationship between milk production and reproduction. Physiological mechanisms that lead to poorer reproduction in high producing cows are partially defined. Negative energy balance that occurs in high producing dairy cows can be associated with a delay in the initiation of ovarian cycles and the interval to first breeding. Many of the effects of negative energy balance on postpartum reproduction can be explained by decreased serum luteinizing hormone (LH) that is associated with negative energy balance. Serum LH increases as cows move toward positive energy balance and greater LH stimulates growth and ovulation of ovarian follicles. We have initiated studies to address physiological differences in high and low index dairy cows. The reproductive endocrinology of cows from a control line (5,900 kg milk/lactation) and a select line (10,900 kg milk/lactation) of dairy cows at the University of Minnesota was studied over a two-year period. Cows in Year 1 were similar for serum concentrations of LH, follicle stimulating hormone (FSH), and oestradiol (preovulatory period). In both years, serum concentrations of progesterone during luteal phases, however, were decreased in select cows. The Year 2 cows also had a delay in the return to oestrous cyclicity that was associated with reduced LH. The possibility that decreased progesterone causes infertility in dairy cows will require further study. Collectively, these data suggest that changes in blood progesterone concentrations may explain, partially, lower fertility in high index dairy cows.

In commercial egg type chicken breeding three and four way crosses are used to produce commercial layers. The primary breeders are using closed nucleus breeding programmes, with birds kept under maximum biosecurity. All grand parents and parents are produced from a closed nucleus for the world wide demand of commercial layers. The breeding goals have been focused for several decades on increasing number of eggs per hen housed. Additional traits have become more important during the last decade, i.e. feed efficiency, internal and external egg quality and general adaptability. Prior to each selection, weights for individual traits within the selection index are adjusted to meet market demands. Breeding stock and commercial layers have to be bred to perform adequately in a variety of systems ranging from large intensive cage units to free range management under different environmental conditions world-wide.

Despite intensive selection for egg production the decrease in genetic variation observed in closed commercial lines is not yet critical. Peak production is approaching the biological limit of one egg a day. During this period genetic and phenotypic variation have been significantly reduced. But for early production (sexual maturity) and late production (persistency) genetic variation is still high. In a mating scheme avoiding full and half sib matings no serious inbreeding depression is observed. To achieve continued future genetic progress, selection pressure will shift to other traits like internal and external egg quality and perhaps behaviour traits which still respond to selection.

Primary breeders are responding to this challenge by testing pedigreed cross-line hens in a wide range of environments and housing systems while the pure-line elite stock is kept under conditions of maximum biosecurity. Marker assisted selection is already part of commercial breeding programmes. In the past, blood typing has been used to improve Marek's resistance, whereas today anonymous microsatellites which are linked to traits of economic interest are used for selection. In particular, selection between full sib males can give a major improvement.

The whole industry is getting more specialised. While the genetic potential of the birds is improved management and nutrition have also to be adapted to changing demands. The general goal for the future is to breed chickens with the ability to function well within a wider range of production conditions and do not respond to the slightest stress.