The short term degradation of ‘Arabian light’ crude oil was followed under various seasonal conditions in coastal seawater at Iles Kerguelen. Artificial degradation experiments were carried out in 3 m3 tanks. In situ experiments were conducted in free-floating, semi-enclosed chambers permitting direct contact between the crude oil and the marine environment. Daily sampling allowed a regular survey of the bacterial changes of the oil contaminated seawater. All samples were analysed for total bacteria, heterotrophic viable microflora and hydrocarbon utilizing microflora. At the end of experiments, the remaining oil was carefully collected for rough quantitative estimation of hydrocarbon degradation. All the results clearly revealed a significant increase in the three types of bacterial microflora after the addition of crude oil. However, the data suggest that the initial state of the bacterial communities is important. Thus, the seasonal variations in the bacterial responses to hydrocarbon addition can be related to the differences in the natural bacterial populations involved. In all cases the wall effects observed in batch systems were reduced with in situ incubations.

Both bacterial culture and molecular approaches were used to investigate the bacterial diversity inside the Chinese Antarctic Great Wall Station and in its adjacent area. Heterotrophic bacteria were isolated from the samples using a direct plating method. γ-Proteobacteria, Actinobacteria, Flavobacteria and Firmicutes were isolated from these samples. In the three water samples, Pseudomonas species were dominant. In soil samples, Flavobacterium, Bacillus or Arthrobacter species dominated. Escherichia coli strains were isolated only in two samples from inside the station. Total cell counts in the six soil samples were semi-quantified by Quantitative Competitive-PCR of the 16S rRNA gene copies. The soil samples contained 105 to 109 cells g−1. Denaturing gradient gel electrophoresis (DGGE) was used further to investigate the bacterial diversity in the soil samples. A wider range of bacterial diversity including α-Proteobacteria, β-Proteobacteria, δ-Proteobacteria, γ-Proteobacteria, Flavobacteria, Actinobacteria and unclassified bacteria was discovered.

Twenty five species of Polychaeta were recorded in soft bottom samples collected from 4–30 m. Total abundance of polychaetes ranged from 60 to 3300 m-2. High abundance values were locally recorded for Microspio moorei, Tharyx epitoca and Ophelina syringopyge. These species, together with more regularly distributed Capitella capitata and Scoloplos marginatus, constituted over 70% of all specimens. Total biomass value of the polychaetes varied between 3.8 and 46.4 gm-2. Travisia kerguelenensis and Aglaophamus ornatus constituted over 75% of total biomass. Species composition, richness and diversity of the polychaete assemblage varied with depth. Two parts of the investigated bottom section, differing in the polychaete assemblages structure, were distinguished; the first one in the depth range from 4–20 m and the second one at the depths of 25–30 m. On the basis of both new and previously published data two types of polychaete assemblages of the shallow soft bottom of the Antarctic sublittoral were distinguished. The type of sediment seems to be the main factor influencing the composition of polychaete assemblages.

The aim of this study was to evaluate the hypothesis that Deschampsia antarctica Desv., the only grass of the Maritime Antarctic, responds to temperatures higher or lower than its optimum for photosynthesis (13°C), with the accumulation of heat shock proteins. The LT50 was determined in plants acclimated at 4°C (cold-acclimated plants) and in plants grown at 13°C (control plants) by membrane damage and by the activity of respiratory dehydrogenases. The LT50 was 48.3°C in both groups of plants when determined by membrane damage. When the LT50 was determined by the activity of respiratory dehydrogenases, this was, without significant difference, 47.8°C in cold-acclimated plants and 46.5°C in control plants. Western blot analyses were performed to investigate the accumulation of HSP70 at different temperatures. The optimal temperature for HSP70 accumulation was 35°C in cold-acclimated and control plants. Cold-acclimated plants subjected to a thermal stress of 35°C accumulated HSP70 protein more than control plants subjected to 35°C. After eight hours of continuous stress at 35°C, the maximum relative content of HSP70 in cold-acclimated plants was 1.9 fold higher than control plants. The threshold temperature for HSP70 accumulation was lower in cold-acclimated plants than in control plants. It is concluded that D. antarctica is able to tolerate heat stress with an LT50 similar to those of heat tolerant plants and that the cold-acclimated plants accumulate higher levels of HSP70 than those grown at 13°C.

A series of benches on nunataks of Alexander Island (Antarctica) are described. An increase in bench size with distance away from the retreating glacier suggests an age spectrum. The benches have thermal contraction cracks (in bedrock) on shallower, upper sections of the risers as well as salt encrusted runnels on the steeper lower section of the tread. The benches also show a distinct orientational preference (orientated to the north through to west) and, from first principles, these seem to be the aspects with optimal freeze-thaw cycles and temperatures conducive to thermal stress fatigue. The extensive dilatation associated with the retreating glaciers is thought to play a significant role in the origin and development of the benches as the combination of extensive jointing and optimal process conditions are thought to constrain where benches begin. The jointing, aided by the thermal contraction cracking, then facilitates extension and continued weathering of the treads. It would appear that these benches are examples of so called “cryoplanation terraces” that have been reported as fossil forms in Europe and North America. The study of such active forms in the Antarctic may provide good analogues for fossil features found in the Northern Hemisphere.

Asterozoans (Echinodermata) of Late Miocene age (6.02 ± 0.12 Ma) are preserved as external moulds in water-lain tuffs of the James Ross Island Volcanic Group (JRIVG), James Ross Island, Antarctic Peninsula. The asterozoans are complete, and appear to represent specimens suffocated after having been pinioned by rapid sedimentation on the distal fringe of an erupting sub-aqueous tuff cone. Although the coarse nature of the host sediments has obliterated the fine morphological detail of the specimens, at least one suggests evidence of entrainment by a turbidity current. A second shows evidence of detachment of the distal tip of one of its arms. In addition to fossil discoveries from glaciomarine sediments, the volcanic tuffs of the JRIVG represent a new source of fossil data that can be used to interpret the ecology and environment of the Antarctic marine shelf biota during the Neogene.

Alkanes and polycyclic aromatic hydrocarbons were quantified in samples of coastal sediments along Fildes Peninsula, King George Island, South Shetland Islands, Antarctica, during the summers of 2005 and 2007. Quantification was done by using GC-MS and applying the EPA 3550B method. Individual polycyclic aromatic hydrocarbon (PAHs) concentrations were below 14.4 ng g-1 dry wt in 2005 and below 88.7 ng g-1 dry wt in 2007. Alkanes concentrations were higher than those of PAHs and ranged from 8 to 2236 ng g-1 dry wt in 2005 and from 53 to 745 ng g-1 dry wt in 2007. Results of total PAHs and alkanes were integrated along with base administrative and geographic maps in a GIS environment to determine the geographic extent of hydrocarbons detected. The largest concentrations were found in areas near research stations where total n-alkanes suggest petrogenic sources and where some diagnostic ratios suggest the presence of some PAHs produced by pyrogenic processes. Even if concentrations of hydrocarbons are low, they seem to be a result of increases in scientific activities, in the activities of the stable population, in the number of tourists, or a combination of theses factors.

This study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l-1. After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l-1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l-1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement (R2 = 0.9785) between experimental and predicted values, with less strong but still good agreement (R2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol.

Enzymatic polymorphism was used to detect variability within Notothenia rossii from two sites on the Kerguelen Plateau and Champsocephalus gunnari from the same sites and the South Orkney Islands. No polymorphism was found in the second species and it was low but not statistically significant in the first. This apparent homogeneity does not substantiate suggestions from other results that the populations can be separated, especially in the case of C. gunnari. Other approaches will be necessary to solve definitively the question of population separation.

We investigated intraspecific geographic variation in buoyancy by obtaining percentage buoyancy (%B) measurements for the Antarctic notothenioid species Pleuragramma antarcticum, Trematomus hansoni, T. bernacchii and Gymnodraco acuticeps from both McMurdo Sound in East Antarctica and the South Shetland Islands in West Antarctica. Mean percentage buoyancies in these species ranged from 0.22–0.52% in the neutrally buoyant P. antarcticum to 3.34–3.67% in the benthic T. bernacchii. Dispersion (1 standard deviation) of percentage buoyancy (%B) values around the mean was ± 0.2–0.5 %B units for the entire sample. Although intraspecific differences in mean percentage buoyancy were statistically significant (P < 0.05) in P. antarcticum and T. hansoni, we consider these differences as normal variation without substantive biological significance. The dispersion in buoyancy measurements during adult life reflects the density of the fish and this may be influenced, in both the short- and long-term, by gut contents, nutritional condition, and reproductive state. Mitigation of the effects of these variables is not biologically realistic because they constitute normal aspects of the daily and yearly life cycles. The results of our measurements of buoyancy are consistent with what is known about the ecology of these four species and this is considered in the discussion.

Epiphytic diatoms are important constituents of the Southern Ocean coastal water ecosystem, being a key element in many of the Antarctic trophic chains. However, only limited information exists relating to these microalgal communities. Here we describe our findings of a study on epiphytic diatoms from Terra Nova Bay (Ross Sea, Antarctica) based on material collected during the summer campaigns spanning from 1990–2004. Observations of diatoms associated with three rhodophyte species (Iridaea cordata (Turner) Bory, Phyllophora antarctica Gepp & Gepp, and Plocamium cartilagineum (L.) Dixon) were carried out with the use of a scanning electron microscope. A total of 73 diatom taxa (32 genera) were distinguished, of which 20 taxa exceeded 3% of total abundance. Cocconeis fasciolata (Ehrenberg) Brown, Navicula perminuta Grunow, and Fragilariopsis nana (Steemann Nielsen) Paasche appeared in every sample. The analysis of similarities (ANOSIM) test as well as non-metric multidimensional scaling analysis indicated the nature of host organism as a major factor influencing associated diatom community structure, whereas depth, site, and time of sampling seemed to be less important. The epizooic communities associated with sessile fauna epiphytic on macroalgae differed significantly from those associated with macroalgal surface. A pronounced difference between the communities epiphytic on various host macroalgae species was also observed, although most of the dissimilarities occurred between diatom taxa of the same growth form.

Tintinnid ciliates were present throughout the upper (100m) water column of the ice-edge zone when sampled in autumn 1986 in the Weddell Sea. Biomass ranged from 0.02 μgC1−1 under the sea-ice to 1.3 μgC1−1 in the ice-free water column. Cymatocylis, Codonellopsis, Laackmaniella and a small Salpingella were the most abundant and/or largest biomass contributors. The under ice assemblage was characterized by low biomass and dominated by small species (Salpingella and Codonellopsis); the ice edge stations were dominated by these same taxa but in higher abundances while the open water assemblage was characterized by high biomass and dominated by Cymatocylis, the largest taxa. All taxa exhibited maximum concentrations in the upper 50m of the water column. Both krill and salps grazed upon the Cymatocylis and Codonellopsis without preference in both the ice covered and open water regimes.

A detailed study was made of the extent of genetic variation within populations of two moss species, Bryum argenteum and Hennediella heimii, from the Garwood Valley in Southern Victoria Land, Antarctica. RAPD (Random Amplified Polymorphic DNA) technology was used to analyse over 30 clumps of each species, collected from adjacent sites in five small meltstream drainage channels. Overall, extensive genetic variation was found, with no two samples being identical in either species. For both species, most clumps showed within-clump variation, although generally the shoots from each clump were most closely related to other shoots from that clump, indicating somatic mutation. Of the B. argenteum isolates, most showed distinct clustering corresponding to the five drainage channels, with some clustering within the top, middle or bottom of the channels, and separation of northern and southern sides of the valley. There was some evidence of across-channel dispersal for B. argenteum. For H. heimii, the situation was quite different; there was very little clustering of clumps according to channel from which they were collected. Rather, the isolates appeared to form one continuous population across the five channels, with partial separation of northern and southern sides of the valley. These results are consistent with the predicted means of dispersal of these species in Antarctica: predominantly by water for B. argenteum, and by wind for H. heimii.

The arrival of iceberg B09B in Commonwealth Bay, East Antarctica, and subsequent fast ice expansion has dramatically increased the distance Adélie penguins (Pygoscelis adeliae) breeding at Cape Denison must travel in search of food. This has provided a natural experiment to investigate the impact of iceberg stranding events and sea ice expansion along the East Antarctic coast. As part of the Australasian Antarctic Expedition 2013–14, the Adélie penguin colony at Cape Denison was censused to compare to historic counts. Whilst some 5520 pairs still bred at Cape Denison there has been an order of magnitude decline in Adélie numbers in the area in comparison to the first counts a century ago and, critically, recent estimates based on satellite images and a census in 1997. In contrast, an Adélie population on the eastern fringe of Commonwealth Bay just 8 km from the fast ice edge was thriving, indicating the arrival of B09B and fast ice expansion was probably responsible for the observed recent population decline. In conclusion, the Cape Denison population could be extirpated within 20 years unless B09B relocates or the now perennial fast ice within the bay breaks out. Our results have important implications for wider East Antarctic if the current increasing sea ice trend continues.

Primary, tail and body moult of three seabirds from Tristan da Cunha archipelago were studied by castnetting offshore south Brazil from February 2006 to August 2007. Timing, duration and synchronization of primary and tail moult are described relative to the annual calendar. Body moult overlapped breeding in Atlantic yellow-nosed albatrosses (Thalassarche chlororhynchos), but tail and primary moult did not. Spectacled petrels (Procellaria conspicillata) had protracted body moult, whereas primary and tail moult were completed by August. We documented onset of primary moult during chick-rearing in spectacled petrels and great shearwaters (Puffinus gravis) of unknown breeding status, and suggest that the south-west Atlantic Ocean holds important numbers of moulting birds of both species during the summer–early autumn. The albatrosses and the spectacled petrels replaced rectrices alternately. Great shearwaters replaced rectrices outward, starting at the central pair. Primary, tail and body moult largely overlap in all three species, suggesting that the metabolic costs of primary moult may not be overly restrictive. Metabolic and nutritional ability to afford simultaneous moult of different feather tracts support the idea that impaired flight caused by wing moult is a strong factor driving no overlap of primary moult and breeding.

Several factors threaten populations of albatrosses and giant petrels, including the impact of fisheries bycatch and, at some colonies, predation from introduced mammals. We undertook population monitoring on Gough Island of three albatross species (Tristan albatross Diomedea dabbenena L., sooty albatross Phoebetria fusca Hilsenberg, Atlantic yellow-nosed albatross Thalassarche chlororhynchos Gmelin) and southern giant petrels Macronectes giganteus (Gmelin). Over the study period, numbers of the Critically Endangered Tristan albatross decreased at 3.0% a year. Breeding success for this species was low (23%), and in eight count areas was correlated (r2 = 0.808) with rates of population decline, demonstrating chick predation by house mice Mus musculus L. is driving site-specific trends and an overall decline. Numbers of southern giant petrels were stable, contrasting with large increases in this small population since 1979. Significant population declines were not detected for either the Atlantic yellow-nosed or sooty albatross, however, caution should be applied to these results due to the small proportion of the population monitored (sooty albatross) and significant interannual variation in numbers. These trends confirm the Critically Endangered status of the Tristan albatross but further information, including a more accurate estimate of sooty albatross population size, is required before determining island wide and global population trends of the remaining species.

The breeding season diet of the three surface-nesting petrel species on the Rauer Islands, Prydz Bay were examined. The stomach contents of cape petrel (Daption capense), Antarctic petrel (Thalassoica antarctica), and southern fulmar (Fulmarus glacialoides) were sampled by collecting regurgitates and by stomach flushing during the 1987/88 early chick-rearing period. Pleuragramma antarcticum and Euphausia superba dominated the diets of all species. By mass they constituted 78% and 22% of Antarctic petrel diet, 63% and 36% of southern fulmar diet, and 14% and 85% of cape petrel diet respectively. P. antarcticum increased in importance in the diet of Antarctic petrels during the course of the study, while E. superba became increasingly important in the diets of southern fulmars and cape petrels. Previous studies conducted during incubation consistently reported the diets as comprising chiefly cephalopods and euphausiids, whilst studies during chick-rearing have reported fish and euphausiids dominating, and very little cephalopod material.

At monthly and annual timescales, the zonally asymmetric circulation over the Southern Ocean is dominated by two quasi-stationary anticyclones: a western anticyclone approximately located south of New Zealand, and an eastern anticyclone located over the Drake Passage region. In this research their influence on late 20th century Antarctic sea ice is explored. During early winter, sea ice in the Weddell, Amundsen and Bellingshausen seas is influenced by the location of the east anticyclone. During late winter, the strength and location of the west anticyclone influences sea ice primarily in the Ross and Amundsen seas. The anticyclones have some effect on wind-driven sea ice motion, but the primary mechanism explaining their link to sea ice appears to be meridional atmospheric thermal advection. A western shift in the west anticyclone may be partly responsible for observed increases in ice cover in the Ross Sea over the late 20th century, but there is little evidence in existing data to support a link between the east anticyclone and observed sea ice trends.

The effect of solar radiation on the survival of Escherichia coli, Salmonella zanzibar and a faecal Streptococcus strain in seawater was tested in laboratory experiments, and survival of E. coli was tested under natural light conditions at Davis Station, Antarctica. Exposure to artificial light of wavelengths 290–800 nm caused a rapid decline in viability of each strain examined. T90 values (the time taken for 90% of the population to be inactivated) were ~50 min, 40 min and 2 h for E. coli, S. zanzibar and the faecal Streptococcus respectively. Selective removal of UV-B wavelengths of (<320 nm) increased survival of E. coli and S. zanzibar, resulting in T90 values of ~2.5 h. Screening out wavelengths of <370 nm (UV-B and most of UV-A) increased T90 values to 5 h for E. coli and 10 h for S. zanzibar. The visible band of the spectrum (400–800 nm) also had deterimental effects on faecal bacteria, when compared with survival in the absence of light. Rates of decline for E. coli in natural light in Antarctica were similar to those from laboratory experiments. However, resuscitation of sub-lethally damaged cells under optimum conditions resulted in greater recovery rates than were observed after exposure to artificial sunlight. Faecal bacteria were rapidly inactivated when exposed to sunlight in Antarctic waters, and as repair mechanisms are unlikely to operate under in situ conditions, resuscitation of sub-lethally damaged cells is improbable.

A dramatic reduction in catch rates of Antarctic toothfish in McMurdo Sound, Antarctica, has led to conclusions that the commercial bottom longline fishery for toothfish in the Ross Sea has drastically altered the toothfish population with cascading effects on the McMurdo Sound ecosystem. However, results from a new monitoring programme for Antarctic toothfish and other top predators carried out in McMurdo Sound in 2014 have shown toothfish catch rate, fish size and fish age similar to those observed prior to 2002. These results suggest that either large and old fish have returned to McMurdo Sound following a temporary environmentally driven absence or that they remained locally present but were not detected in the areas sampled. These findings highlight the importance of continued standardized monitoring for detecting the potential effects of fishing on the Ross Sea ecosystem.