Ecotoxicological studies with cladocerans frequently assess the individual growth of the organisms. Measures upon living organisms should be avoided once they can lead to their death or affectation, therefore constraining the results. In this way, and considering that cladocerans species go through series of moults along their life cycle, measures upon the moult are preferred in almost all the studies. Thus, the establishment of allometric relations is required to enable feasible estimations of diverse growth parameters. Exopodite and total body length of two autochthonous (Daphnia longispina; Ceriodaphnia pulchella) and two standard (Daphnia magna; Ceriodaphnia dubia) living daphnids were registered. Regression analysis was applied in order to establish a relation between the two allometric measurements in each species. As high R-squared values were reached in all species, the obtained equations can be used as reference for estimations of body length in face of the «moult» exopodite length measure. Consequently, this work provides a support reference for ecotoxicological studies performed in lab with these species.

1. Cinq espèces d'Elmidae typiques de ruisseaux furent étudiées et chacune se développe sur un substrat particulier : Stenelmis crenata aux endroits sablonneux, Optioservus ampliatus parmi les gravillons, Macronychus glabratus sur les bois pourris immergés, Promoresia tardella dans les mousses aquatiques et Oulimnius latiusculus sur les fonds de gravier mêlés avec du sable.

2. Ce sont des microphaghes qui se nourrissent de débris végétaux (30-90%) et de Diatomées (10-70 %).

3. Le nombre de stades larvaires varie d'une espèce à l'autre : 8 chez S. crenata, 7 chez O. ampliatus, 6 chez M. glabratus et P. tardella et 5 chez O. latiusculus.

4. Les stades larvaires se distinguent surtout par la longueur du prothorax ou du 9e segment abdominal et par l'ornementation tégumentaire.

5. La longueur minimale du cycle biologique de ces espèces semble être de trois années, peut-être quatre même chez S. crenata, dont deux à l'état larvaire (trois chez S. crenata) et une à l'état adulte.

6. Les oeufs sont pondus au cours de l'été et la majorité éclosent en août.

7. La première année, les larves se développent jusqu'au stade III et passent l'hiver à ce stade, car à 0 °C, la croissance est arrêtée ou du moins extrêmement ralentie.

8. Les larves atteignent leur plein développement du printemps à l'automne de la deuxième année et passent de nouveau l'hiver à l'état larvaire.

9. Ces larves de dernier stade quittent l'eau au printemps pour la nymphose qui a lieu de juin au début d'août, sous les débris végétaux du bord de l'eau.

10. Les imagos émergent surtout en août et doivent attendre jusqu'à l'été de la troisième année avant de se reproduire.

The growth and cell composition of C. meneghiniana in P-, N- or Si-limited continuous cultures under different dilutions are discussed. Carbon, nitrogen and phosphorus content per cell or per unit cell volume increased with growth rate in all chemostats. Maximum growth rate depends on the type of limiting nutrient. The maximum growth rates are. 1.65 ± 0.04, 0.73 ± 0.07 and 0.95 ± 0.08 d-1 for P-, N- and Si-limited cultures respectively. These values are close to those calculated by Droop, Caperon, Goldman and Monod models. The concentrations of N and P in the river Danube are much higher than the requirements for growth and uptake of C. meneghiniana. This indicates that neither P nor N are limiting to the growth of C. meneghiniana in natural habitat.

The distribution and overall fish abundance, and biomass of fish populations in the open water of meso-eutrophic Lake Balatonwere studied in 2003-2007 using hydroacoustics and gill nets. Gillnet catches revealed that bleak (53-76 %) common bream (8-42%), white bream and razor fish (2-18 %) dominated in the open water of the lake. Comparisons of target strength distribution fromhydroacoustics and length distribution from the gillnets showed that the fishing gear did not quantitatively catch the smallest andlargest size groups of fish. Mean acoustic fish abundance and biomass fluctuated significantly between both basins and years. Thebiomass of fish populations reflects the trophic gradient along the longitudinal axis of the lake (min-max.: 89-181 kg ha-1) showinga decreasing trend with years. The mean acoustic fish biomass was significantly higher in the eutrophic western basin, comparedwith mesotrophic eastern and middle ones.

Microcystis blooms occur worldwide in eutrophic lakes. Microcystins (MCs) including microcystin-LR (MC-LR) released by Microcystis have adverse effects on aquatic organisms such as rotifers. To detect population growth and reproductive responses, the rotifer Brachionus calyciflorus was exposed to MC-LR at eight concentrations ranging from 0 to 200 μg.L−1 under different temperature (20, 25 and 30°C), and population growth rate (r), ovigerous females/non-ovigerous females (OF/NOF) ratio, mictic females/amictic females (MF/AF) ratio, mictic rate (MR), and 7-d resting egg (7-d RE) production were investigated. The results showed that higher temperatures stimulate the population growth of B. calyciflorus. B. calyciflorus showed high tolerance to MC-LR at concentrations lower than 200 μg.L−1 under different temperatures. Compared to the control, MC-LR at all concentrations increased the r (P<0.05), but decreased the OF/NOF and the MR of the rotifers at 30°C (P<0.01). A clear dose–response relationship existed between the r, the OF/NOF, and the MR of B. calyciflorus and MC-LR concentration at 30°C, respectively. These sensitive parameters could be used to monitor the ecological effects of low concentrations of MC-LR in natural water bodies at the high temperature.

The role of predation in the regulation of freshwater communities is predicted to decrease along a habitat-duration gradient, from permanent to episodic waters. We tested the role of invertebrate predation in shaping the community structure in a fishless temperate intermittent pond with a three month long hydroperiod by comparing the community structure in two large field enclosures (4.2 m2) with added predators to two enclosures without added predators. The added predators reflected the density and composition of top predators in the pond and comprised weekly additions of dytiscid larvae (for three weeks) followed by weekly additions of odonate nymphs (for five weeks). Compared with the enclosure controls, the predator addition enclosures had fewer dipterans and crustaceans, higher concentrations of benthic ciliates and other protozoans, higher chlorophyll a and bacterial counts, and lower abundance of rotifers. Many treatment effects were temporally variable and this appeared to be linked to predator identity, predator size, and prey availability. Compared with the surrounding pondwater, the enclosed areas had lower abundance of molluscs, ostracods and cladocerans but higher abundance of cyclopoids and higher concentrations of phytoplankton and ciliates. Despite high productivity and seasonally variable predator and prey assemblages, which likely buffered against strong top-down control, we conclude that the top-predators regulate the dipterans and zooplankton in this intermittent pond and that the effects propagated down through the food web to lower trophic levels.

La durée de vie et le taux net de reproduction (Ro) sont mesurés à 20 °C et avec une alimentation optimale chez 8 souches de Rotifères Monogonontes Pseudotroques, appartenant à 6 espèces différentes : Asplanchna brightwelli, Brachionus calyciflorus, Epiphanes brachionus, Notommata copeus, Polyarthra dolichoptera et Synchaeta pectinata. Les durées de vie et taux net de reproduction respectifs des femelles amictiques et des femelles mictiques fécondées et non fécondées sont comparés chez les quatre premières espèces. La durée de vie des femelles amictiques varie de 5 à 12 jours selon les espèces ou les souches d'une même espèce, de 5 à 8 jours pour les femelles mictiques non fécondées. A l'exception d'Asplanchna brightwelli, le nombre d'oeufs (femelles) pondus par une femelle amictique — soit de 13 oeufs ♀-1 chez Polyarthra dolichoptera à plus de 40 oeufs ♀-1 chez Epiphanes senta — est à peu près équivalent au nombre d'oeufs (mâles) pondus par une femelle mictique. Le taux net de reproduction dépend non seulement de la température mais aussi de l'alimentation qui n'a que peu d'influence sur la durée de vie.

In the natural environment, microbial community structure of river biofilm is controlled by biotic and abiotic factors. This study explored the capacity to manipulate the structure of microbial communities by modifying environmental conditions during the course of biofilm development. River epilithic biofilm was cultivated in situ on artificial substrates placed parallel to river water flow. Substrates were incubated for 3 and 5.5 weeks in river to allow natural biofilm development, at two sites with contrasting physico-chemical characteristics. The first site (Auradé, Gers, France) was located in an agricultural watershed basin and the second site (Larroque, Haute-Garonne, France) was located in a forested watershed basin. After 3 weeks of biofilm development, a subset of substrates was collected from one site and transplanted to the second site where they remained for 2.5 further weeks. Epilithic bacterial community structure (at 3 weeks from each site and at 5.5 weeks from biofilms with and without transplantation) was assessed using PCR-DGGE of 16S rDNA fragment. Biofilm biomass was estimated using ash free dry mass (AFDM). After 3 weeks of development, biofilms from the two sites exhibited comparable AFDM values (average of 1.4±0.2 g.m-2). A difference between the two sites was observed after 5.5 weeks of development: AFDM decreased for biofilms from the agricultural watershed basin (from 1.4 to 0.18 g.m-2) as a consequence of grazing pressure (Bithynia), and increased for biofilms from the forested agricultural watershed (from 1.4 to 2.6 g.m-2). Microbial community analyses revealed a differentiated community structure between biofilms from the different sites and exhibited a change of microbial community structure after 5.5 weeks of biofilm development. These observations confirm a process of ecological succession in microbial communities. Changing the incubation site during biofilm development modified the trajectory of these ecological successions, suggesting that site characteristics mainly conditioned the structure of these microbial communities.

Using field experiments, we investigated the effects of fish carcasses (so called ‘food falls') on benthic fauna. We simulated food falls using freshly killed fish during two different seasons (spring and summer) in the littoral zone of a large, pre-alpine meso-oligotrophic lake in central Europe (Lake Constance, Germany). This study provides evidence that input in the form of fish carcasses may play an important role in nutrient dynamics within this ecosystem. The benthic communities in the vicitinity and underneath the food fall were strongly influenced by the food fall. The results show that this supply of organic matter has a significant influence on the relationships within the communities, which are clearly dominated by bacteria, followed by copepod nauplii, cyclopoid copepods, chironomids, and ostracods. Total decomposition was obtained between days 80 and 68 of the experiments. The food fall had a positively but not significant effect on meiofaunal assemblages in these experiments. Furthermore, the results showed a negative relationship between bacteria and meiofauna abundance, indicating grazing by the meiobenthos on the bacterial community. These findings support other studies that found that the meiofauna exerted a grazing pressure on the microbial community, where this process was important in the decomposition of carcasses. Moreover, this study shows the potential importance of fish carcasses in Lake Constance, where food falls may generate high abundances and diversity of the benthic fauna and support high bacterial activities in this littoral ecosystem.

Tropical island streams worldwide are being threatened by existing or proposed dams and diversions. Numerous streams of the Hawaiian Islands have diversions that remove freshwater for human use; however, little research has addressed diversion effects on aquatic insect communities in tropical streams. We studied the effect of water removal by a stream diversion on density and biomass of two introduced Trichoptera, Cheumatopsyche analis (Banks) (Hydropsychidae) and Hydroptila potosina (Buenoa-Soria) (Hydroptilidae), in Iao Stream, Maui, Hawaii, from April-August 2000. Both species seem to have multivoltine life cycles. Pooled Trichoptera (both species) biomass was significantly greater above the diversion (178.3 mg.m-2 and 112.9 mg.m-2 for upstream and downstream, respectively). For each individual species the mean total instar biomass was significantly reduced below the diversion (38 % and 54 % reduction for C. analis and H. potosina, respectively). Both species had greater densities above the diversion; however, individual larval mass of H. potosina instars, but not C. analis instars, was significantly lower below the diversion. Our results suggest that reduced habitat quality, due to lowered stream flow, limits density of both species below the diversion, whereas lowered food quantity and quality may differentially affect H. potosina biomass through reduced body sizes. These data suggest that water removal not only reduces habitat quality, but significantly lowers the community biomass of these introduced Trichoptera species, affecting the trophic energetics of stream reaches downstream of a diversion in Hawaii. Similar changes to macroinvertebrate communities would be expected below dams and diversions of streams in other tropical regions.

The population dynamics of the red swamp crayfish (Procambarus clarkii Girard, 1852) was studied from May 2005 to May 2006 in a pond (PCD) subjected to drought between June and December and in a continuously flooded rice field drainage channel (PMG) located in the Tejo river basin, Portugal. The proportion of females in the population was not independent from the season and was negatively correlated with both female and male maturation status. Male and female maturation was synchronous but males remained in a reproductive form after recruitment while females reverted quickly to lower maturation indexes. At PCD and PMG, respectively 2 and 5 cohorts were detected by modal analysis. Large crayfish were abundant in spring, summer and early autumn and juveniles were abundant in autumn, especially at PCD. Crayfish density at PCD was lower than at PMG and mortality rate (Z) was 3.99 at PCD and 2.43 at PMG. The average lifespan of the crayfish populations was 91 days at PCD and 150 days at PMG. Our study indicated faster growth and larger maximum sizes at the site with the more stressed environment and lowest relative crayfish densities (PCD), suggesting that any exploitation or control strategies for these two populations should consider management of the hydroperiod and of maximum crayfish densities.

La part prise par les aliments végétaux décroît dans la série Arcynopteryx -Isoperla - Périodes - Perla et Dinocras. Des différences morphologiques sont liées à cette évolution. Les régimes alimentaires des Isoperla, Perla et Dinocras varient au cours de la vie larvaire. Les relations entre la taille du prédateur et celle des proies sont plus nettes pour les Baetis que pour les Chironomides. Pour les Chironomides, elles sont de plus en plus marquées dans la série Arcynopteryx - Isoperla - Perlidae.

To test whether leaf litter from eucalyptus plantations affects stream macroinvertebrate communities, we manipulated litterinputs into a small headwater tributary of the Agüera stream (northern Spain) running under mixed deciduous forests. Threecontiguous 50 m long reaches were used: the upstream reach was left as a control (site C), whereas the two downstream siteswere covered by 1 mm mesh nets to avoid natural litter inputs, and twice a month received litter collected at a deciduous forest(site D, mid) or a eucalyptus plantation (site E, downstream). Benthic Surber samples were taken from the three sites before andafter the treatment and density, biomass and structure of the community were compared. Density and biomass of macroinvertebratesdid not differ among sites before the treatment. Total density and biomass of macroinvertebrates increased in all the sitesduring the experimental period but most considerably at the site were deciduous forest inputs were simulated (station D). At theend of the experiment shredders were significantly less abundant at site E (eucalyptus) than at site D (deciduous). In addition,shredder density and biomass per total organic matter amount and per total leaf amount became higher at site D. In conclusion,these results suggest a subtle negative impact of eucalyptus litter on stream macroinvertebrate communities, especially on shredders.

During 2001 - 2002, we studied periphytic biomass at 5 sites along the Agüera stream (Northern Iberian Peninsula), and comparedthe results to those from two previous researches (1990 - 1991 and 1992 - 1993). Sampling sites showed large differencesin cover, conductivity and nutrient contents, as well as some inter-annual differences, resulting from changes in discharge andin human activities. Most environmental variables showed low seasonality, main variations being produced by floods. Solarradiation changed little during 2001 - 2002, because of the coincidence of a sunny winter followed by a cloudy summer, andseasonal differences in riparian cover resulted in more sunlight reaching the streambed of closed sites during winter, and smalldifferences at the open ones. The growth and abscission of riparian leaves results in a strong light regime at the closed sites, withmore sunlight during winter, but this pattern changes from year to year, depending on the weather. Spatial differences and highestvalues of periphytic biomass were much lower than those measured in 1990 - 91, because of contrasting weather patterns.Variables controlling periphytic biomass in the Agüera change from reach to reach. At open sites flow is the main temporalcontroller, whereas at closed sites the effects of light availability prevail, thus giving more similar seasonal patterns from yearto year. Nutrients seem to play a minor role in temporal variations.

Weight loss of leaves of Populus nigra and Quercus ilex was observed in coarse-mesh bags exposed in three watercourses and their sediment (-30 and -50 cm) near the city of Fes. The watercourses were: Oued Sebou (a river), Oued Sidi-Harazem (issuing from a warm spring), and Ain Chkef (a cold spring). Weight loss was faster at the surface than within the sediment. Weight loss was faster in poplar than in the tough holm oak leaves. At all levels, weight loss was fastest in Oued Sebou where the water flowed faster both at the surface and within the coarser sediment. It was slowest in the cold spring. Thus the influence of leaf texture, current velocity, and temperature were stressed.

Présentation, sous forme de catalogue, des principaux renseignements tirés de l'étude des 204 espèces et sous-espèces de Trichoptères récoltés sur le versant nord des Pyrénées. Ce travail est basé sur des captures d'adultes effectuées ces dernières années, ainsi que sur l'étude des collections de Trichoptères pyrénéens du Muséum d'Histoire Naturelle de Paris et du British Museum.

Les principaux travaux de faunistique sont indiqués, avec les dates et lieux de capture.

Pour chaque espèce sont signalés :

1) les citations antérieures,

2) la classe d'abondance,

3) la répartition dans les vallées nord-pyrénéennes,

4) les mois de capture des adultes,

5) l'habitat et l'amplitude altitudinale,

6) des remarques d'ordre systématique.

35 espèces — citations douteuses ou inexactes, synonymes — ont été écartées de la liste des Trichoptères pyrénéens.

Les mois de capture des adultes sont indiqués dans un tableau concernant l'ensemble des espèces. Les formes à longue période de vol sont relativement nombreuses.

L'étude des captures d'adultes dans trois stations différentes montre une contraction des périodes de vol en montagne, avec un seul maximum, en juillet-août, au-dessus de 1 400 m.

Les modifications subies par les périodes de vol en fonction de l'altitude sont différentes pour les espèces des faunes précoce, intermédiaire et tardive.

La faune précoce de haute montagne paraît très appauvrie dans les Pyrénées; la faune automnale survit jusqu'en décembre à des altitudes élevées.

Surface runoff can supply lakes with a variety of chemical substances – their type and quantity may significantly vary and depend on the characteristics of the catchment area: geomorphology, phytocoenosis type and degree of human impact. In this study we investigated the physicochemical properties of the surface runoff water collected from the wooded catchment of Lake Piaseczno Duze (LPD, Drawa National Park, Poland) covered by a monoculture of Scots pine (Pinus sylvestris) and mixed forest with white birch (Betula pubescens) as the dominant tree. Experimentally, we also aimed to study the leaching of nutrients from pine and birch litter. Throughout the investigated period runoff waters had low pH, brown colour and high levels of dissolved organic carbon (DOC) – most likely induced by humic acids. Furthermore, considerable levels of nitrogen (N) and phosphorus (P) were found. The highest concentrations of nutrients were observed in runoff collected after heavy rainfall and snow melting. Runoff from the coniferous area contained significantly higher levels of DOC but lower concentrations of N and P compared to runoff collected from the birch-dominated forest. Similar physicochemical conditions were observed in the leaching experiment. Moreover, it was found that the release of chemical substances from both coniferous and deciduous litter was rapid. Our study indicates that surface runoff from forest areas can significantly affect lake chemistry. Based on the simultaneous analyses of littoral water chemistry we suggest that it may contribute to LPD dystrophication through the transportation of high levels of acidic compounds.

The aim of this research was to investigate three main movement patterns of meiofauna and macrofauna in the riverbed: (1) vertical (downwards) within the interstitial habitat; (2) downstream (negative rheotaxis); (3) upstream (positive rheotaxis). The study was conducted in two headwater streams in the Northern Apennines (Italy), during summer 2009. Sets of traps opening upwards, upstream and downstream to collect, respectively, organisms moving down into the sediment, and organisms with negative and positive rheotaxis, were placed in each sampling site. Benthic samples were collected as well, to compare the benthic community composition with the assemblages colonizing the traps. Meiofauna was the dominant component, representing 95% in benthos and 85% in traps. Vertical top-opened traps collected more taxonomic groups and more individuals of macro- and meiofauna than the horizontal traps, suggesting a dominance of movements deep within the substrate rather than horizontal patterns. Horizontal traps opening upstream (negative rheotaxis) were colonized by more individuals than the traps opening downstream (positive rheotaxis), demonstrating the great importance of movements directed downstream as a primary source of colonization of new areas. Temporary meiofauna (i.e., insect larvae), which was the dominant component of trap assemblages, displayed predominantly vertical movements, supporting the nursery and refuge function of the hyporheic habitat for taxa which spend only the early larval stages in the hyporheos. The results also stress the importance of including meiofauna in studies characterizing the lotic communities.

Corbicula fluminea populations were sampled annually from 1997 to 2000 at the beginning of the autumn, i.e. at the end of the reproduction period, at 14 sites along the Saône and Rhône rivers. Recruitment in the upper reach of these rivers was generally high. Conversely, the observation of other sectors revealed generally low or no production of juveniles, missing cohorts and major year to year variations in population structure. Nevertheless, no dramatic fall in population density was observed during the surveyed four years. This suggests that the longevity of the Corbicula populations of the Saône and Rhône rivers is ensured to a great extent by the drifting of individuals from the upper course of these rivers and their tributaries. Two possible causes are put forward to explain the low recruitment observed : low production of phytoplankton that prevents adults from constituting sufficient energy reserves to ensure the full development of larvae, and/or toxic contamination, which inhibits the development of phytoplankton and it is lethal for pediveligers.

The objectives were to evaluate temporal variation in 137Cs in trout and charr from Crummock Water, Wastwater, Ennerdale Water, and to compare levels in trout from six lakes (above three + Windermere, Loweswater, Devoke Water) and charr from four lakes (above three + Windermere). Fish were caught between June 1986 and October 1988, chiefly with fyke nets and gill nets. Overall means for 137Cs in trout were markedly higher than those for charr in the three lakes. Values for individual trout exceeded 1000 Bq.kg-1 in Ennerdale and 500 Bq.kg-1 in Wastwater and Crummock Water, but values for charr never exceeded 350 Bq.kg-1. 137Cs content increased with fish weight for trout from Wastwater and therefore values were scaled to a standard weight in subsequent analyses. No similar relationships were found for trout in Ennerdale or Crummock Water, or for charr in all three lakes. Monthly mean values for 137Cs in trout followed similar temporal patterns in the three lakes and a parabola provided a simple model for these changes. Chernobyl deposition occurred early in May 1986 and maximum values in trout were in December 1986 in Wastwater, January/February 1987 in Ennerdale Water and March 1987 in Crummock Water. The exponential rate of decrease from these maxima did not differ significantly between the three lakes, and ecological half-lives of 137Cs in the trout differed slightly, but not significantly, between lakes : 180 days for Wastwater, 194 days for Crummock Water and 249 days for Ennerdale Water. The equivalent value for Ennerdale charr was lower at 132 days, and it was impossible to fit models or calculate half-lives for Wastwater and Crummock charr. Maximum monthly geometric mean 137Cs values in trout from six lakes and charr from four lakes were related to the initial concentration of 137Cs in both water and sediment, and the maximum monthly geometric mean 137Cs values obtained from the routine water samples. The relationship was curvilinear and approximated, but was not identical to, an asymptotic curve. No similar relationships were found with mean Ca or K levels in the lake water. Metabolic and diet differences may explain the lower 137Cs levels in charr compared with trout and differences for the same species from different lakes may be due to limnological variations and différences between lakes in the nature of their sediments and catchment soils. Both points are discussed in detail.