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Effects of excess metabolizable protein on ovarian function and circulating amino acids of beef cows: 2. Excessive supply in varying concentrations from corn gluten meal

Published online by Cambridge University Press:  09 September 2016

T. C. Geppert ,
A. M. Meyer ,
G. A. Perry  and
P. J. Gunn
T. C. Geppert
Affiliation:
Department of Animal Science, Iowa State University, Ames, IA 50011, USA
A. M. Meyer
Affiliation:
Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA
G. A. Perry
Affiliation:
Department of Animal Sciences, South Dakota State University, Brookings, SD 57007, USA
P. J. Gunn*
Affiliation:
Department of Animal Science, Iowa State University, Ames, IA 50011, USA
*
†Email: [email protected]
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Abstract

In the dairy industry, excess dietary CP is consistently correlated with decreased conception rates. However, amount of excess CP effects on reproductive function in beef cattle is largely undefined. The objective of this experiment was to determine the effects of excess metabolizable protein (MP) supplementation from a moderately abundant rumen undegradable protein (RUP) source (corn gluten meal: 62% RUP) on ovarian function and circulating amino acid (AA) concentrations in beef cows consuming low quality forage. Non-pregnant, non-lactating beef cows (n=16) were allocated by age, BW and body condition score (BCS) to 1 of 2 isocaloric supplements designed to maintain BW for 60 days. Cows had ad libitum access to corn stalks and were individually offered a corn gluten meal-based supplement daily at 125% (MP125) or 150% (MP150) of National Research Council (NRC) MP requirements. After a 20-day supplement adaptation period, cows were synchronized for ovulation. After 10 days of synchronization, follicular growth was reset with gonadotropin releasing hormone. Daily thereafter, transrectal ultrasonography was performed to diagram ovarian follicular waves, and blood samples were collected for hormone, metabolite and AA analyses. After 7 days of observation of estrus, corpus luteum (CL) size was determined via ultrasound. Data were analyzed using the MIXED procedures of SAS. No differences (P⩾0.21) in BW and BCS existed throughout the study; however, plasma urea N at ovulation was greater (P=0.04) in MP150. Preovulatory ovarian follicle size at dominance, duration of dominance, size at spontaneous luteolysis, length of proestrus and wavelength were not different (P⩾0.11) between treatments. However, ovulatory follicles were larger (P=0.04) and average antral follicle count was greater (P=0.01) in MP150 than MP125. Estradiol concentration and ratio of estradiol to ovulatory follicle volume were not different due to treatment (P⩾0.25). While CL volume 7 days post-estrus was greater (P<0.01) in MP150 than MP125, circulating progesterone 7 days post-estrus and ratio of progesterone to CL volume were not different (P⩾0.21). Total AA were not different (P⩾0.76) at study initiation or completion; however, as a percent of total AA, branched-chain AA at ovulation were greater (P=0.02) in MP150. In conclusion, supplementation of CP at 150% of NRC MP requirements from a moderately undegradable protein source may enhance growth of the ovulatory follicle and subsequent CL compared with MP supplementation at 125% of NRC MP requirements.

Keywords

branched-chain amino acidsbeef cowfollicleplasma urea nitrogenprotein
Type
Research Article
Information
animal , Volume 11 , Issue 4 , April 2017 , pp. 634 - 642
Copyright
© The Animal Consortium 2016 

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Footnotes

a Present address: South Dakota State University Extension, 1800 E. Spruce St., Mitchell, SD 57301, USA.

References

Atkins, JA, Smith, MF, MacNeil, MD, Jinks, EM, Abreu, FM, Alexander, LJ and Geary, TW 2013. Pregnancy establishment and maintenance in cattle. Journal of Animal Science 91, 722–733.CrossRefGoogle ScholarPubMed
Bridges, GA, Ahola, JK, Brauner, C, Cruppe, LH, Currin, JC, Day, ML, Gunn, PJ, Jaeger, JR, Lake, SL, Lamb, GC, Marquezini, GHL, Peel, RK, Radunz, RE, Stevenson, JS and Whittier, WD 2012. Determination of the appropriate delivery of prostaglandin F2α in the five-day CO-Synch + controlled intravaginal drug release protocol in suckled beef cows. Journal of Animal Science 90, 4814–4822.CrossRefGoogle ScholarPubMed
Bridges, GA, Mussard, ML, Burke, CR and Day, ML 2010. Influence of length of proestrus on fertility and endocrine function in female cattle. Animal Reproduction Science 117, 208–215.CrossRefGoogle ScholarPubMed
Busch, DC, Atkins, JA, Bader, JF, Schafer, DJ, Patterson, DJ, Geary, TW and Smith, MF 2008. Effect of ovulatory follicle size and expression of estrus on progesterone secretion in beef cows. Journal of Animal Science 86, 553–563.CrossRefGoogle ScholarPubMed
Butler, WR, Calaman, JJ and Beam, SW 1996. Plasma and milk urea nitrogen in relation to pregnancy rate in lactating dairy cattle. Journal of Animal Science 74, 858–865.CrossRefGoogle ScholarPubMed
Ciccioli, NH, Wettemann, RP, Spicer, LJ, Lents, CA, White, RF and Keisler, DH 2003. Influence of body condition at calving and postpartum nutrition on endocrine function and reproductive performance of primiparous beef cows. Journal of Animal Science 81, 3107–3120.CrossRefGoogle ScholarPubMed
Cushman, RA, Allan, MF, Kuehn, LA, Snelling, WM, Cupp, AS and Freetly, HC 2009. Evaluation of antral follicle count and ovarian morphology in crossbred beef cows: Investigation of influence of stage of the estrous cycle, age and birth weight. Journal of Animal Sciene 871, 971–1980.Google Scholar
Cushman, RA, McNeel, AK and Freetly, HC 2014. The impact of cow nutrient status during the second and third trimesters on age at puberty, antral follicle count, and fertility of daughters. Livestock Science 162, 252–258.CrossRefGoogle Scholar
Echternkamp, SE, Cushman, RA and Allan, MR 2009. Size of ovulatory follicles in cattle expressing multiple ovulations naturally and its influence on corpus luteum development and fertility. Journal of Animal Science 87, 3556–35568.CrossRefGoogle ScholarPubMed
Elrod, CC and Butler, WR 1993. Reduction of fertility and alteration of uterine pH in heifers fed excess ruminally degradable protein. Journal of Animal Science 71, 694–701.CrossRefGoogle ScholarPubMed
Geppert, TC, Meyer, AM, Perry, GA and Gunn, PJ 2016. Effects of excess metabolizable protein on ovarian function and circulating amino acids of beef cows: 1. Excessive supply from corn gluten meal or soybean meal. Animal, published online doi:10.1017/S1751731116001889.CrossRefGoogle Scholar
Ginther, OJ, Kot, K, Kulick, LJ and Wiltbank, MC 1997. Emergence and deviation of follicles during the development of follicular waves in cattle. Theriogenology 48, 75–87.CrossRefGoogle ScholarPubMed
Ginther, OJ, Silva, LA, Araujo, RR and Beg, MA 2007. Temporal associations among pulses of 13,14-Dihydro-15-keto-PGF2alpha, luteal blood flow, and luteolysis in beef cattle. Biology of Reproduction 76, 506–513.CrossRefGoogle Scholar
Greiwe, JS, Kwon, G, McDaniel, ML and Semenkovich, CF 2001. Leucine and insulin activate p70 S6 kinase through different pathways in human skeletal muscle. American Journal of Physiology, Endocrinoloy, and Metabolism 281, E466–E471.CrossRefGoogle ScholarPubMed
Gunn, PJ, Lemenager, RP and Bridges, GA 2014a. Using corn stover and dried distillers grains with soluble to conserve stockpiled forages and improve reproductive performance and progeny growth in fall-calving beef cows. Professional Animal Scientist 30, 215–224.CrossRefGoogle Scholar
Gunn, PJ, Lemenager, RP and Bridges, GA 2014b. Excess rumen undegradable protein alters parameters of reproductive function in beef cows. Animal Industry Report AS 660, ASL R2852. Retrieved on 5 May 2015 from http://lib.dr.iastate.edu/ans_air/vol660/iss1/17.Google Scholar
Gunn, PJ, Schoonmaker, JP, Lemenager, RP and Bridges, GA 2014c. Feeding excess crude protein to gestating and lactating beef heifers: Impact on parturition, milk composition, ovarian function, reproductive efficiency and pre-weaning progeny growth. Livestock Science 167, 435–448.CrossRefGoogle Scholar
Ireland, JJ, Smith, GW, Scheetz, D, Jimenez-Krassel, F, Folger, JK, Ireland, JLH, Mossa, F, Lonergan, P and Evans, ACO 2011. Does size matter in females? An overview of the impact of the high variation in the ovarian reserve on ovarian function and fertility, utility of anti-Mllerian hormone as a diagnostic marker for fertility and causes of variation in the ovarian reserve. Reproduction, Fertility and Development 23, 1–14.CrossRefGoogle Scholar
Jinks, EM, Smith, MF, Atkins, JA, Pohler, KG, Perry, GA, MacNeil, MD, Roberts, AJ, Waterman, RC, Alexander, LJ and Gear, TW 2012. Preovulatory estradiol and the establishment and maintenance of pregnancy in suckled beef cows. Journal of Animal Science 91, 1176–1185.CrossRefGoogle Scholar
Lemley, CO, Camacho, LE, Meyer, AM, Kapphahn, M, Caton, JS and Vonnahme, KA 2013. Dietary melatonin supplementation alters uteroplacental amino acid flux during intrauterine growth restriction in ewes. Animal 7, 1500–1507.CrossRefGoogle ScholarPubMed
Lents, CA, White, FJ, Ciccioli, NH, Wettemann, RP, Spicer, LJ and Lalman, DL 2008. Effects of body condition score at parturition and postpartum protein supplementation on estrous behavior and size of the dominant follicle in beef cows. Journal of Animal Science 86, 2549–2556.CrossRefGoogle ScholarPubMed
McNatty, KP, Gibb, M, Dobson, C, Thurley, DC and Findlay, JK 1981. Changes in the concentration of gonadotrophic and steroidal hormones in the antral fluid of ovarian follicles throughout the oestrous cycle of the sheep. Australian Journal of Biological Science 34, 67–80.CrossRefGoogle Scholar
National Research Council (NRC) 2000. Nutrient requirements of beef cattle, 7th revised edition. National Academy Press, Washington, DC, USA.Google Scholar
Perry, GA and Perry, BL 2008. Effect of preovulatory concentrations of estradiol and initiation of standing estrus on uterine pH in beef cows. Domestic Animal Endocrinology 34, 333–338.CrossRefGoogle ScholarPubMed
Perry, GA, Smith, MF, Lucy, MC, Green, JA, Parks, TE, MacNeil, MD, Roberts, AJ and Geary, TW 2005. Relationship between follicle size at insemination and pregnancy success. Proceedings of the National Academy of Science of the USA 102, 5268–5273.CrossRefGoogle ScholarPubMed
Perry, GA, Smith, MF, Roberts, AJ, MacNeil, MD and Geary, TW 2007. Relationship between size of the ovulatory follicle and pregnancy success in beef heifers. Journal of Animal Science 85, 684–689.CrossRefGoogle ScholarPubMed
Rensis, FD and Scaramuzzi, RJ 2003. Heat stress and seasonal effects on reproduction in the dairy cow – a review. Theriogenology 60, 1139–1151.CrossRefGoogle ScholarPubMed
Rusche, WC, Cochran, RC, Corah, LR, Stevenson, JS, Harmon, DL, Brandt, RT Jr. and Minton, JE 1993. Influence of source and amount of dietary protein on performance, blood metabolites, and reproductive function of primiparous beef cows. Journal of Animal Science 71, 557–563.CrossRefGoogle ScholarPubMed
Silva, JR, Figueiredo, VJR and Van Den Hurk, R 2009. Involvement of growth hormone (GH) and insulin-like growth factor (IGF) system in ovarian folliculogenesis. Theriogenology 71, 1193–1208.CrossRefGoogle ScholarPubMed
Tamanini, C, Basini, G, Grasselli, F and Tirelli, M 2003. Nitric oxide and the ovary. Journal of Animal Science 81 (suppl. 2), E1–E7.Google Scholar
Tamanini, C and De Ambrogi, M 2004. Angiogenesis in developing follicle and corpus luteum. Domestic Animal Reproduction 39, 206–216.CrossRefGoogle ScholarPubMed
Vasconcelos, JLM, Sartori, R, Oliveira, HN, Guenther, JG and Wiltbank, MC 2001. Reduction in size of the ovulatory follicle reduces subsequent luteal size and pregnancy rate. Theriogenology 56, 307–314.CrossRefGoogle ScholarPubMed
Wagner, JJ, Lusby, KS, Oltjen, JW, Kakestraw, J, Wettermann, RP and Walters, LE 1988. Carcass composition in mature Hereford cows: estimation and effect on daily metabolizable energy requirement during winter. Journal of Animal Science 66, 603–612.CrossRefGoogle ScholarPubMed
Zhang, Z, Debing, Y, Dingzhong, Y and Zhengchao, W 2011. Activation of PI3K/mTOR signaling pathway contributes to induction of vascular endothelial growth factor by hCG in bovine developing luteal cells. Animal Reproduction Science 125, 42–48.CrossRefGoogle ScholarPubMed