A new endoparasitic monogenean of Paradiplectanotrema Gerasev, Gayevskaya & Kovaleva, 1987, Paradiplectanotrema klimpeli sp. nov., is described from the southern Balinese coast, Indonesia. The new species is much larger, wider and characterized by the longest dorsal anchors compared with the congeners. Ventral anchors and ventral bars are the smallest in the genus, with a distinct ratio of 1:1. This is the first species with a gladiator breast-plate-shaped dorsal bar, with a length:width ratio of 1:1. Oesophagi of the Common Grinner Saurida tumbil (Bloch, 1795) (Synodontidae) were infected (prevalence = 17%) at an intensity of 12 (1–21). This is the first record of the genus from the eastern Indian Ocean, and lizardfishes represent a new host family. We provide light microscopy (in situ in oesophagal folds), three-dimensional confocal illustrations and a morphometric comparison of all congeners, with remarks on the recently described first Indonesian endoparasitic Monogenea Pseudempleurosoma haywardi Theisen, Palm, Al-Jufaili & Kleinertz, 2017. First 28S DNA sequences for Paradiplectanotrema allocate the new species close to endoparasitc freshwater monogeneans. Its ecology differs from Pseudempleurosoma Yamaguti, 1965 by utilizing deep-water fishes instead of coastal, coral reef-associated hosts; however, both are infecting schooling, bottom-dwelling fishes.

Eimeria maxima has been used as a model apicomplexan parasite to study sexual stage development and oocyst wall formation. A complete understanding of the wall's biochemical and biophysical properties is of great interest in research on all apicomplexan parasites. Purified gametocytes, zygotes and oocysts were analysed by three-dimensional confocal microscopy, and wide-field fluorescent microscopy was used to investigate the appearance and spatial organization of the 2 types of wall-forming bodies (WFBs). In addition, a variety of staining procedures and immunoassays were used to assess the biosynthesis, metabolic activity, intactness and molecular composition of the WFBs in situ. WFBs were extracted from gametocytes/zygotes and their composition was assessed by microscopy and SDS-PAGE analysis. It was concluded that isolated gametocytes are intact and metabolically active. Additionally, it was observed that the Type 1 WFBs are aligned at the periphery of the parasite and fuse together producing neutral lipid rich patches that appear to be inserted into the space between 2 parasite-specific membranes. Finally, it was shown that the WFBs extracted from purified gametocytes had the same shape, size and staining properties as those observed in situ, and contained the major glycoprotein antigens known to be present in these organelles.