Throughout the Ediacaran Period, variable water-column redox conditions persisted along productive ocean margins due to a complex interplay between nutrient supply and oceanographic restriction. These changing conditions are considered to have influenced early faunal evolution, with marine anoxia potentially inhibiting the development of the ecological niches necessary for aerobic life forms. To understand this link between oxygenation and evolution, the combined geochemical and palaeontological study of marine sediments is preferable. Located in the Yangtze Gorges region of southern China, lagoonal black shales at Miaohe preserve alga and putative metazoans, including Eoandromeda, a candidate total-group ctenophore, thereby providing one example of where integrated study is possible. We present a multi-proxy investigation into water-column redox variability during deposition of these shales (c. 560–551 Ma). For this interval, reactive iron partitioning indicates persistent water-column anoxia, while trace metal enrichments and other geochemical data suggest temporal fluctuations between ferruginous, euxinic and rare suboxic conditions. Although trace metal and total organic carbon values imply extensive basin restriction, sustained trace metal enrichment and δ15Nsed data indicate periodic access to open-ocean inventories across a shallow-marine sill. Lastly, δ13Corg values of between −35‰ and −40‰ allow at least partial correlation of the shales at Miaohe with Member IV of the Doushantuo Formation. This study provides evidence for fluctuating redox conditions in the lagoonal area of the Yangtze platform during late Ediacaran time. If these low-oxygen environments were regionally characteristic, then the restriction of aerobic fauna to isolated environments can be inferred.

The introduction of agriculture is a key defining element of the Neolithic, yet considerable debate persists concerning the nature and significance of early farming practices in north-west Europe. This paper reviews archaeobotanical evidence from 95 Neolithic sites (c. 4000–2200 cal bc) in Wales, focusing on wild plant exploitation, the range of crops present, and the significance of cereals in subsistence practices. Cereal cultivation practices in Early Neolithic Wales are also examined using cereal grain stable carbon (δ13C) and nitrogen (δ15N) isotope analysis. The Early Neolithic period witnessed the widespread uptake of cereals alongside considerable evidence for continued wild plant exploitation, notably hazelnuts and wild fruits. The possibility that wild plants and woodlands were deliberately managed or altered to promote the growth of certain plants is outlined. Small cereal grain assemblages, with little evidence for chaff and weed seeds, are common in the Early Neolithic, whereas cereal-rich sites are rare. Emmer wheat was the dominant crop in the Early Neolithic, while other cereal types were recorded in small quantities. Cereal nitrogen isotope (δ15N) values from Early Neolithic sites provided little evidence for intensive manuring. We suggest that cultivation conditions may have been less intensive when compared to other areas of Britain and Europe. In the later Neolithic period, there is evidence for a decline in the importance of cereals. Finally, the archaeobotanical and crop isotope data from this study are considered within a wider European context.

The introduction of agriculture is known to have profoundly affected the ecological complexion of landscapes. In this study, a rapid transition from C3 to C4 vegetation is inferred from a shift to higher stable carbon (13C/12C) isotope ratios of soils and sediments in the Benoué River Valley and upland Fali Mountains in northern Cameroon. Landscape change is viewed from the perspective of two settlement mounds and adjacent floodplains, as well as a rock terrace agricultural field dating from 1100 cal yr BP to the recent past (<400 cal yr BP). Nitrogen (15N/14N) isotope ratios and soil micromorphology demonstrate variable uses of land adjacent to the mound sites. These results indicate that Early Iron Age settlement practices involved exploitation of C3 plants on soils with low δ15N values, indicating wetter soils. Conversely, from the Late Iron Age (>700 cal yr BP) until recent times, high soil and sediment δ13C and δ15N values reflect more C4 biomass and anthropogenic organic matter in open, dry environments. The results suggest that Iron Age settlement practices profoundly changed landscapes in this part of West Africa through land clearance and/or utilization of C4 plants.

The population size of anadromous salmon in the Pacific Northwest is strongly influenced by decadal variation in watershed and oceanographic conditions and therefore should also be influenced by larger magnitude millennial-scale variations in these conditions. We studied δ15N of bulk organic matter in lake sediment from Woahink Lake, Oregon, as a proxy of marine-derived nutrients (MDN) from spawning coho salmon. We compared this site to a control lake with a natural barrier to salmon migration. From 7.5 to 5.4 ka, a dune was emplaced, breached, and emplaced again, forming Woahink Lake in a former estuary. δ15N decreased steadily since 5.4 ka at Woahink but not at the control lake. δ15N reached a minimum just prior to anthropogenic nutrient loading, which caused an increase in δ15N, thus precluding a comparison with the historical decline in salmon abundance. A mixing model of lake-water nitrate, developed to explore alternate scenarios for the observed range of δ15N, could not explain these results without invoking MDN input from at least several hundred salmon annually. Our results show a previously unreported pattern of a millennial-scale decline in salmon that has plausible linkages to parallel changes in ocean circulation and productivity.

Massive to lobate volcanic flows and brecciated hyaloclastite units in the Abitibi greenstone belt allow investigation of Late Archæan seafloor alteration and associated incorporation into these rocks of nitrogen (N) biogeochemical signatures. In this suite (the Blake River Group), hyaloclastite units containing putative microbial ichnofossils are particularly enriched in large-ion lithophile elements (K, Rb, Ba, Cs), B, and Li, consistent with their having experienced the greatest fluid–rock interaction during subseafloor hydrothermal alteration. Similarly, silicate-δ18O and δ15N values for samples from the hyaloclastites show the greatest shifts from plausible magmatic values. The chemical and isotopic patterns in these tholeiitic igneous rocks greatly resemble those in modern altered seafloor basalts, consistent with the preservation of an Archæan seafloor alteration signature. The N enrichments and shifts in δ15N appear to reflect stabilization of illite and interaction with fluids carrying sedimentary/organic signatures. Enrichments of N (and the δ15N of this N) in altered glass volcanic rocks on Earth's modern and ancient seafloor point to the potential utility of N for tracing past and present biogeochemical processes in similar rocks at/near the Mars surface.

This study uses two novel archaeobotanical techniques – crop carbon and nitrogen isotope analysis and functional weed ecology – to determine directly how the intensity of agricultural practice changed from the Neolithic to the Early Iron Age in south-west Germany, with the emergence of fortified hilltop settlements (Fürstensitze or chiefly seats) regarded as the first urban centres in central Europe. The crop isotope and functional weed ecological evidence suggest that surplus cereal production in the Early Iron Age was achieved through sustained use of manure combined with expansion in arable cultivation, both developments that are connected with more widespread use of animal traction. The increased scale of cultivation is broadly apparent across rural as well as fortified hilltop centres in the Early Iron Age, and considerable variability in manuring intensity is consistent with agricultural decision-making at a local level rather than centralised control. Additionally, the more intensive manuring of hulled six-row barley, used in beer production, demonstrates that the political importance of drinking and feasting in Early Iron Age society was reflected in crop husbandry practices. In terms of animal husbandry, faunal isotope data reveal a radical decrease in forest cover, potentially reflecting an expansion in the scale of herding accompanying that of arable cultivation. Site-specific patterning points to a range of herding strategies, from specialised herding of cattle at the Heuneburg to generalised patterns of livestock management at rural sites.

Lake Bosumtwi is one of the most widely studied palaeoclimate archives in West Africa. Results from numerous AMS 14C dates of samples from four piston cores from Lake Bosumtwi show that an abrupt sedimentary transition from a mid-Holocene sapropel to calcareous laminated muds occurred at about 3200 cal yr B.P. High-resolution analyses of the nitrogen isotopic composition of organic matter across this transition confirm its abrupt nature, and suggest that the change may signal a step toward increased aridity and intensified surface winds that affected western equatorial Africa from Ghana to the Congo basin. Northern and Eastern Africa experienced a similar abrupt shift toward aridity during the late Holocene, but at about 5000 cal yr B.P., a difference in timing that illustrates the regional nature of climate changes during the Holocene and the importance of feedback mechanisms in regulating Holocene climate variability. Furthermore, an abrupt change at about 3000 cal yr B.P. occurs at several sites adjacent to the tropical and subtropical Atlantic, which may hint at major changes in the surface temperatures of the tropical Atlantic and/or Pacific at this time.

Trees growing on the Mt. Sedom salt diapir, at the southern Dead Sea shore, were swept by runoff into salt caves and subsequently deposited therein, sheltered from surface weathering. A subfossil Tamarix tree trunk, found in a remote section of Sedom Cave is radiocarbon dated to between ∼ 2265 and 1930 BCE. It was sampled in 109 points across the tree rings for carbon and nitrogen isotopes. The Sedom Tamarix demonstrates a few hundred years of 13C and 15N isotopic enrichment, culminating in extremely high δ13C and δ15N values. Calibration using modern Tamarix stable isotopes in various climatic settings in Israel shows direct relationship between isotopic enrichment and climate deterioration, particularly rainfall decrease. The subfossil Tamarix probably reflects an environmental crisis during the Intermediate Bronze Age, which subsequently killed the tree ∼ 1930 BCE. This period coincides with the largest historic fall of the Dead Sea level, as well as the demise of the large regional urban center of the 3rd millennium BCE. The environmental crisis may thus explain the archaeological evidence of a shift from urban to pastoral culture during the Intermediate Bronze Age. This was apparently the most severe long-term historical drought that affected the region in the mid-late Holocene.

We reconstructed late Holocene fluctuations of Kluane Lake in Yukon Territory from variations in bulk physical properties and carbon and nitrogen elemental and isotopic abundances in nine sediment cores. Fluctuations of Kluane Lake in the past were controlled by changes in climate and glaciers, which affected inflow of Slims and Duke rivers, the two largest sources of water flowing into the lake. Kluane Lake fluctuated within a narrow range, at levels about 25 m below the present datum, from about 5000 to 1300 cal yr BP. Low lake levels during this interval are probably due to southerly drainage of Kluane Lake to the Pacific Ocean, opposite the present northerly drainage to Bering Sea. Slims River, which today is the largest contributor of water to Kluane Lake, only rarely flowed into the lake during the period 5000 to 1300 cal yr BP. The lake rose 7–12 m between 1300 and 900 cal yr BP, reached its present level around AD 1650, and within a few decades had risen an additional 12 m. Shortly thereafter, the lake established a northern outlet and fell to near its present level.

The high-quality organic preservation at Alpine lakeshore settlement sites allows us to go beyond simplistic reconstructions of farming in the Neolithic. The rich archaeological datasets from these sites may be further complemented by methods such as nitrogen isotope (δ15N) analysis of charred crop remains. At Hornstaad-Hörnle IA and Sipplingen, on the shore of Lake Constance in south-west Germany, this method has been used to provide a unique insight into strategies of cultivation such as manuring on both a spatial and temporal scale.

What benefits were derived from the invention of pottery, and why did ceramics remain marginal for so long? The increasing use of pottery has been seen as a response to large-scale harvesting in a model that favours economic advantage through increased efficiency. This paper challenges that view; combining carbon and nitrogen isotope and lipid analysis, the authors argue that pottery was used selectively for storing or processing valued exchange commodities such as fish oil. Its use can be seen as part of broader developments in hunter-gatherer society, featuring seasonal gatherings, collective feasting and a new articulation of social relations.

Nitrous oxide (N2O) is a potent greenhouse gas and the dominant anthropogenic stratospheric ozone-depleting emission. The tropospheric concentration of N2O continues to increase, with animal production systems constituting the largest anthropogenic source. Stable isotopes of nitrogen (N) provide tools for constraining emission sources and, following the temporal dynamics of N2O, providing additional insight and unequivocal proof of N2O source, production pathways and consumption. The potential for using stable isotopes of N is underutilised. The intent of this article is to provide an overview of what these tools are and demonstrate where and how these tools could be applied to advance the mitigation of N2O emissions from animal production systems. Nitrogen inputs and outputs are dominated by fertiliser and excreta, respectively, both of which are substrates for N2O production. These substrates can be labelled with 15N to enable the substrate-N to be traced and linked to N2O emissions. Thus, the effects of changes to animal production systems to reduce feed-N wastage by animals and fertiliser wastage, aimed at N2O mitigation and/or improved animal or economic performance, can be traced. Further 15N-tracer studies are required to fully understand the dynamics and N2O fluxes associated with excreta, and the biological contribution to these fluxes. These data are also essential for the new generation of 15N models. Recent technique developments in isotopomer science along with stable isotope probing using multiple isotopes also offer exciting capability for addressing the N2O mitigation quest.

Sweet lupin (Lupinus albus), a protein-rich legume devoid of anti-nutritional factors, is considered to have a high potential for protein nutrition in man. Results concerning the nutritional value of lupin protein are, however, conflicting in animals and very scarce in human subjects. Furthermore, where fibre-rich protein sources are concerned, the long-term nutritional results are often obscured, particularly since fibre-promoted colonic fermentation may bias the energy supply and redistribute N flux. We therefore studied, during the postprandial phase, the bioavailability and utilisation of lupin-flour protein in nine healthy men who had ingested a mixed meal containing intrinsically 15N-labelled lupin flour as the protein source (Expt 1). The real ileal digestibility (RID) and ileal endogenous N losses (IENL) were assessed using a perfusion technique at the terminal ileum, and the N content and 15N enrichment of ileal samples. Lupin flour exhibited a high RID of 91 (SD 3) % AND LOW IENL (5·4 (sd 1·3) mmol N/h). Postprandial dietary deamination was also assessed from body dietary urea and urinary dietary N excretion, and compared with results in nine healthy men following an isoenergetic meal containing a 15N-soyabean-protein isolate with a similar RID, as a control (Expt 2). Postprandial dietary deamination was similar after lupin and soyabean meals (17 (sd 2) and 18 (sd 4) % ingested N respectively). We therefore conclude that lupin protein is highly bioavailable, even if included in fibre-rich flour, and that it can be used with the same efficiency as soyabean protein to achieve postprandial protein gain in healthy human subjects.