Acanthocheilonema viteae, Litomosoides carinii and Setaria cervi were found to actively synthesize proteins in vitro. Different centrifugation fractions and their TCA-precipitable fractions were assessed for the distribution of newly synthesized proteins. Penicillin and streptomycin inhibited the process in A. viteae. The synthesis in S. cervi was susceptible to puromycin, chloramphenicol, cycloheximide, neomycin and polymyxin B. The process in L. carinii was strongly blocked by puromycin while chloramphenicol had no significant effect.