Michelia lacei W.W. Smith, a magnolia species categorized as Endangered on the IUCN Red List, is subject to severe disturbance. We carried out field surveys and a review of literature records to present a detailed description of the current status of M. lacei. We then predicted the potential distribution of M. lacei under different climatic scenarios based on 60 occurrence records (53 recorded during our field surveys and 7 earlier records) and 19 bioclimatic variables from the WorldClim database. We selected 18 locations and four bioclimatic variables for model training. Temperature seasonality and annual temperature range were the most influential variables for predicting the potential distribution of the species. We used MaxEnt to model distribution under current climate conditions and four Shared Socioeconomic Pathway scenarios in four future time periods to determine the effects of future climate change on the habitat suitable for the species. We predict areas of moderately and highly suitable habitat will gradually decrease over time. We recommend increased in situ and ex situ conservation efforts to mitigate this habitat decline and protect populations of M. lacei.

Effective ex situ conservation of plants in botanic gardens requires sufficient wild accessions to represent wild diversity. In Rhododendron L. (Ericaceae), c. 64% of the taxa are threatened or require field investigation. As a case study of the analysis of ex situ conservation gaps we used ecogeographical representation as a proxy for genetic representation in ex situ collections of the 65 taxa of Rhododendron subsection Maddenia. We compiled the first list profiling both wild distributions and ex situ wild collections of all taxa in subsection Maddenia. Our results reveal that 55 Maddenia taxa are in cultivation. Of the 18 threatened taxa all are in cultivation but nine require further collection to capture adequate wild diversity. There are 12 Data Deficient taxa: these await further field investigation of wild populations and nine of them require wild collections to conserve genetic diversity. The UK, the USA, Australia, New Zealand and China are the top five countries holding ex situ collections of subsection Maddenia; in these countries nearly 66% of the ex situ sites hold > 86% of the global living collections of subsection Maddenia. We recommend that wild collections of endemic species of subsection Maddenia should be established in all 10 countries of origin and that data should be shared internationally for metacollections. In addition to proposing priorities, our case study highlights the challenges facing data and collection management to help achieve effective ex situ conservation for Rhododendron species.

Living plant collections play a crucial role in ex situ conservation, but their conservation value hinges on assessment of their composition, data quality and on strategic planning for optimization of the collection that takes into account species diversity, conservation status and allocation of resources. Cactaceae is one of the most threatened plant families globally and has a broad distribution in Brazil, with two of the seven centres of diversity for this group and a total of 276 known species. Climate change could escalate the threat to cacti, potentially placing 90% of species at risk by 2050. We conducted a case study of Cactaceae in the living Cactarium collection of the Rio de Janeiro Botanic Garden, evaluating the diversity of the collection and recommending strategic actions to enhance its conservation value. In 2022, we inventoried all specimens in the collection and cross-referenced them with the institutional specimen registry system, Jabot. The collection contains 156 cacti species that naturally occur in Brazil (57% of the total), including 43 species categorized as threatened on the Brazilian Red List and 57 categorized as threatened on the IUCN Red List. We recommend research to map cacti species that are threatened but not covered by in situ conservation in the country, list the priority species for acquisition by the collection, and make recommendations that will facilitate improved contribution of the Rio de Janeiro Botanic Garden to the conservation of the Brazilian flora.

The Malabar dwarf puffer, Carinotetraodon travancoricus is the smallest known pufferfish (family Tetraodontidae) and one of the smallest freshwater fishes of the Indian subcontinent. Due to their miniature size, wacky behaviour and appearance, they are much preferred in the international aquarium fish trade, although little is known regarding their breeding activity in captivity and their embryonic development. The purpose of this study was to fill these knowledge gaps. Wild-caught Malabar dwarf puffers were acclimatised to conditions, and pairs were introduced to breeding tanks. Adult fishes were fed with live and frozen diets including Artemia nauplii, moina and bloodworm. During spawning seasons, adult fish displayed elaborate courtship behaviour around sunset. Carinotetraodon travancoricus is a batch spawner releasing 1 to 5 eggs per diem. The eggs were spherical, and non-sticky, with a diameter of 1.48 ± 0.1 mm, and hatching took place after 108 to 116 h post-incubation. The newly hatched larvae were 3.5 ± 0.2 mm in length, and weighed 2.9 ± 0.4 mg. The early larvae have substantial yolk and oil globules as an energy reserve. Histological studies on mature females suggested the batch spawning nature of the species and low fecundity. Given its unique reproductive behaviour and characters, in situ protected habitats are required to ensure their continued survival in the wild, apart from encouraging captive breeding to augment the demand in the international aquarium fish trade.

Pear (Pyrus communis L.) stands out as a prominent fruit species in temperate regions worldwide. The Çoruh River basin, nestled in the lower Caucasus in Türkiye, serves as a valuable repository of pear germplasm. To elucidate the genetic structure of pear populations in this region, 84 village pear cultivar genotypes (land races) from six villages, sample garden collections (SCC), and wild Panta root stock populations were analysed using eleven microsatellite markers. Genetic diversity and structure analyses indicated that village pear cultivar populations exhibit substantial genetic diversity and admixture. This diversity is attributed to local farming practices such as phenotypic selection and widespread dispersal of clonal materials. The genetic structure analysis, combined with the identification of private alleles, indicates that the pear genetic resources in the Çoruh river basin likely has originated from two gene pool sources, specifically the Meydancık and Camili village pear traditional cultivar populations. The Camili village pear cultivar population as a new in situ genetic reserve site has been proposed. Despite the existence an ex situ conservation site, the study suggests inadequateness of SCC as an ex situ site in capturing the full extent of genetic diversity of village pear cultivar genetic resources. Thus, enriching the genetic diversity in the SCC ex situ site is essential for effective pear genetic resource conservation in the Çoruh river basin. These findings contribute valuable insights for the development of targeted conservation strategies, ensuring the preservation of pear genetic resources in this region.

Variable harlequin frogs Atelopus varius have declined significantly throughout their range as a result of infection with the fungal pathogen Batrachochytrium dendrobatidis (Bd). The Panama Amphibian Rescue and Conservation Project maintains an ex situ population of this Critically Endangered species. We conducted a release trial with surplus captive-bred A. varius individuals to improve our ability to monitor frog populations post-release, observe dispersal patterns after freeing them into the wild and learn about threats to released frogs, as well as to determine whether natural skin toxin defences of frogs could be restored inside mesocosms in the wild and to compare Bd dynamics in natural amphibian communities at the release site vs a non-release site. The 458 released frogs dispersed rapidly and were difficult to re-encounter unless they carried a radio transmitter. No frog was seen after 36 days following release. Thirty frogs were fitted with radio transmitters and only half were trackable by day 10. Tetrodotoxin was not detected in the skins of the frogs inside mesocosms for up to 79 days. Bd loads in other species present at sites were high prior to release and decreased over time in a pattern probably driven by weather. No differences were observed in Bd prevalence between the release and non-release sites. This trial showed that refinements of our methods and approaches are required to study captive Atelopus frogs released into wild conditions. We recommend continuing release trials of captive-bred frogs with post-release monitoring methods, using an adaptive management framework to advance the field of amphibian reintroduction ecology.

Behavioural management in zoos is often practiced within the confines of environmental enrichment, a well-known method which attempts to increase the welfare of captive animals. For the successful conservation and reintroduction of threatened or endangered species, however, it is also important to manage behaviour in such a way as to maintain behavioural diversity. The development of natural behaviour management (NBM) programs is advocated in this paper. These programs will act to maintain behavioural diversity in captivity and will encourage behaviour to be displayed in appropriate contexts through exposure of captive animals to naturalistic stimuli. The importance of developing appropriate antipredator and predation behaviours will be discussed in order to demonstrate how NBM strategies differ from, and can even conflict with, environmental enrichment strategies undertaken for welfare reasons.

Ex situ conservation of species is risky and expensive, but it can prevent extinction when in situ conservation fails. We used the IUCN Guidelines on the Use of Ex Situ Management for Species Conservation to evaluate whether to begin ex situ conservation for the South-east Asian subspecies of Bengal florican Houbaropsis bengalensis blandini, which is predicted to be extinct in the wild within 5 years. To inform our decision, we developed a decision tree, and used a demographic model to evaluate the probability of establishing a captive population under a range of husbandry scenarios and egg harvest regimes, and compared this with the probability of the wild population persisting. The model showed that if ex situ conservation draws on international best practice in bustard husbandry there is a high probability of establishing a captive population, but the wild population is unlikely to persist. We identified and evaluated the practical risks associated with ex situ conservation, and documented our plans to mitigate them. Modelling shows that it is unlikely that birds could be released within 20–30 years, by which time genetic, morphological and behavioural changes in the captive population, combined with habitat loss and extinction of the wild population, make it unlikely that Bengal florican could be released into a situation approximating their current wild state. We considered the philosophical and practical implications through a decision tree so that our decision to begin ex situ management is not held back by our preconceived notions of what it means to be wild.

UK trees require increased conservation efforts due to sparse and fragmented populations. Ex situ conservation, including seed banking, can be used to better manage these issues. We conducted accelerated ageing tests on seeds of 22 UK native woody species, in order to assess their likely longevity and optimize their conservation in a seed bank. Germination at four ageing time points was determined to construct survival curves, and it was concluded that multiple samples within a species showed comparable responses for most species tested, except for Fraxinus excelsior. Of all species studied, one could be classified as very short-lived, four as short-lived and 17 as medium, with none exceeding the medium category. The most important finding of this manuscript is that although some taxonomic trends were observed, the results indicate the need for caution when making broad conclusions on potential seed storage life at a species, genus or family level. Longevity predictions were compared to actual performance of older collections held in long-term storage at the Millennium Seed Bank, Kew. Although most collections remain high in viability in storage after more than 20 years, for short-lived species at least, there is some indication that accelerated ageing predicts longevity in seed bank conditions. For species with reduced potential longevity, such as Fagus sylvatica and Ulmus glabra, additional storage options are recommended for long-term gene banking.

Seeds are increasingly being stored in seed banks for ex situ conservation, food security, and restoration reserves. Although it is hypothesized that seeds of many species can survive the dry, freezer conditions (−20°C) of seed banks for many years, shelf life is unknown for many species. In particular, changes to dormancy and germination requirements following freezer storage have not yet been studied extensively. To improve our understanding of how seed dormancy and germination respond to seed banking conditions, we evaluated seed of six restoration-priority species in the southwestern United States, which is a region of increasing restoration need. Germination tendencies of seeds that were and were not exposed to a −20°C treatment for 4–6 months were evaluated under 22 cold-moist stratification and incubation treatments to broadly assess changes to dormancy and germination requirements and speed. Direct or indirect (via interactions with stratification and/or incubation treatments) differences in dormancy breaking and germination were observed in seeds for four of the six species studied. Specifically, storage temperature accounted for differences in the final germination proportion, germination during stratification, and/or rate of germination in five of the six species. Notably, seeds of the one species that exhibit a combination of physiological and physical dormancy showed significant differences in all germination measures. However, while significant differences were found between seeds exposed to short-term seed bank storage and those that were not, these differences were small and may not be biologically meaningful, indicating that seed banking these species should not change how they are used for restoration or reintroduction purposes.

Genebanks explore new partnerships with farmers and other user groups to provide smallholder farmers in Africa better access to crop diversity for improved nutrition, climate change adaptation and agricultural diversification. This paper shows how the World Vegetable Center (WorldVeg) genebank of traditional African vegetables and its partners distributed over 42,000 seed kits containing over 183,000 vegetable seed samples from 2013 to 2017 to smallholder farmers in Tanzania, Kenya and Uganda. The seed kits contained seed samples of promising accessions and open-pollinated breeding lines of traditional African vegetables, and to a lower degree of tomato, Capsicum pepper and soybean, usually enough to plant in a home garden. We identified four research questions to better understand the role of vegetable seed kits in strengthening local seed systems, impact on local vegetable diversity, improving human nutrition and supporting climate-resilient agriculture. As formal seed systems expand their reach, the genebank's role to supply vegetable diversity to public and private breeding programmes becomes more important. To optimize supply of vegetable diversity, the WorldVeg genebank of traditional African vegetables continues working with partners in both formal and local seed systems.

Crop wild relatives (CWR) are valuable resources for crop breeding due to their close genetic relationship to the cultivated plants and their wide genetic variation, allowing the introgression of desirable traits into the crops, such as resistance to plant pests and diseases or adaptability to climate change. Mexico is a centre of agrobiodiversity, including CWR, but climate change, and other factors, are contributing to the loss of important Mexican CWR genetic diversity. The in situ and ex situ conservation status of Mexican priority CWR were assessed through a gap analysis as part of a national CWR conservation strategy for Mexico, to ensure the long-term preservation and improve the availability of these genetic resources. A set of 310 priority CWR taxa, previously identified as part of the national CWR inventory for Mexico, were analysed. Species distribution modelling and ecogeographic diversity analyses were used to detect gaps in in situ and ex situ conservation at taxon and ecogeographic levels. Priority target sites were identified throughout the country for complementary in situ and ex situ conservation of these taxa. The results obtained allow us to make recommendations for immediate conservation actions, thus helping to mitigate the threats to Mexican agrobiodiversity and enhance both national and global food security.

Crop wild relatives (CWR) are valuable gene pools for crop improvement and offer unique potential and opportunity for enhancing food security and adaptation to climate change. However, current actions towards conservation of plant genetic resources in Zambia do not adequately cover CWR occurring in the country. The article describes the process leading to the development of a national strategic action plan (NSAP) for the conservation and sustainable use of priority CWR in Zambia. Based on 59 prioritized crops, a partial checklist of 459 CWR taxa was generated from the national flora checklist of 6305 taxa. The generated CWR taxa were prioritized based on the socio-economic value of the related crop, their utilization potential in crop improvement, relative distribution and threat status to produce 30 prioritized CWR taxa. Occurrence data were compiled for all CWR inventory taxa and used in spatial analyses to establish species distribution, species richness, gaps in in situ conservation and genebank collections, and to identify priority sites for in situ conservation and ex situ collecting. Consistent with the national developmental agenda, along with the contribution of national stakeholders, spatial analyses of occurrence data of priority CWR taxa are valuable input for the development of the NSAP for the conservation and sustainable use of the priority CWR.

Exotic crops—plant species grown in relatively small quantities and not traditionally cultivated in a country or region—are often intimately linked with the ethnic origins of their maintainers and are a principal source of culinary and nutritional diversity for many people. Recognizing that a wealth of exotic crop diversity and associated knowledge is held by small-scale growers in the UK, Garden Organic initiated the Sowing New Seeds project to capture and preserve some of this valuable resource by building a seed collection and knowledge base. To establish a sample of this diversity and knowledge, we undertook a survey at 31 allotment sites in the Midlands region of the UK with the objectives of identifying the exotic crops cultivated, characterizing the demography of those who grow them, understanding their direct use values, and assessing their potential indirect use value for the diversification and improvement of other crops. Results reveal that 26% of the food crops recorded are exotic and that they are grown by people belonging to 13 different ethnic groups. The majority save their own seed, indicating that these crops are performing well in the UK, with grower selection providing the basis for their continuing success. Further, most maintainers swap seed with other growers, indicating that exotic crops are likely to be gradually diversifying in response to different growing conditions—a positive sign for their value for local food security and as national genetic resources with potential for use in crop improvement programs. The research highlights the multitude of benefits that growers obtain through cultivating exotic crops, which are not only related to nutrition and culinary requirements, but also to general health and well-being, culture, and a range of other forms of life enrichment. It is critical that growers are encouraged and supported in continuing to cultivate, save and pass on their exotic crops to younger generations, as well as to protect allotments from development in order to maintain this important diversity adapted to local growing conditions. Importantly, many exotic crops currently grown on a small scale may enter into commerce, and thus expand the diversity of the UK's food crop base. Such a shift may be particularly important in the face of the increasingly detrimental impacts of climate change on crop production. We conclude that exotic crop diversity could be more important for future nutrition, health and food security than we currently appreciate.

The valorization of genetic diversities of major crops like wheat may help substantially to feed the world Population. Durum wheat genotypes consisting of 265 farmers’ varieties (FVs), which have been cultivated for many centuries in Ethiopia, as well as 24 improved varieties (IMVs) have been recently evaluated in northern Ethiopia. The evaluation has been carried out at two different locations for 2 consecutive years to verify the inherited diversity in FVs for important phenological and agronomic traits; with the intention to provide refined information to breeders and genebank managers. As a result of a careful evaluation, a very significant variation was observed between the FVs and IMVs. A large number of the former have demonstrated superior performance to the latter in terms of mean values of the major traits within the stipulated years and locations. The best performing FV has shown a gain of 20% grain yield over the best IMV. Multivariate analyses revealed that FVs displayed larger genetic diversity than in those IMVs. FVs could therefore be used as donor of useful alleles in durum wheat breeding for improvement of yield per se and other traits of agronomic and phenological importance. The identified stable superior FVs include: 8208, 226834A, 238567, 222426, 226282 could be best candidates for farmers in marginal environments. Genotypes that have shown stable performance for spatial variation such as 204493A, 214357 and 238567; and temporal variation such as 8208, 208479, 214357 and 226834A could be the best candidates for exploitation in future breeding programs.

Magnolia sinica, a Critically Endangered tree endemic to Yunnan, China, is one of the 20 plant species with extremely small populations approved by the Yunnan government for urgent rescue action before 2015. Information on the geographical distribution and population size of this species had not previously been reported, hindering effective conservation. We therefore carried out a survey of the literature and of herbarium specimens, followed by a detailed field survey and morphological measurements and observations of surviving individuals. We located 52 individuals in the wild, in eight localities. Two distinguishing morphological characters (tepal colour and tepal number) were revised based on observations of all remaining wild individuals that produced flowers and on one 30-year-old flowering plant in Kunming Botanical Garden. The survival rate of individuals propagated from seed for ex situ conservation at the Garden was 100% over 5 years; of 100 individuals transplanted to each of two reinforcement sites, 20 and 18, respectively, were alive after 6 years. We propose two groups of measures to protect M. sinica: (1) in situ conservation, population monitoring, and public engagement, and (2) ex situ conservation with reinforcement or reintroduction.

This investigation aimed to evaluate the quality of cryopreserved semen from 21 Curraleiro Pé Duro bulls in a germplasm bank, categorizing the doses of the sires according to parameters related to sperm viability, and verifying the fertilizing capacity in vitro of the spermatozoids. All the samples were thawed and analyzed as to morphology, kinetics, plasma membrane integrity and acrosome integrity. Samples of only four bulls were used for evaluating the fertilization rates. The results indicated that the Curraleiro Pé Duro semen doses, stocked in the Brazilian Animal Germplasm Bank, showed variable sperm quality and high in vitro fertilizing capacity, making it possible to design appropriate strategies for assisted reproduction in order to conserve this Brazilian genetic resource, which is in danger of extinction.

Wenchengia alternifolia (Lamiaceae) is a plant species endemic to Hainan Island, China. Since the 1930s it was known from only four collections and was believed to be extinct until a remnant population was rediscovered in 2010. We conducted further field surveys during 2010–2012 but located only one population, with 66 individuals. W. alternifolia is restricted to the c. 1,500 m2 Shuangximu Valley, in a harsh microenvironment surrounded by plantations. As the population comprises < 50 mature individuals, we propose that W. alternifolia be categorized as Critically Endangered on the IUCN Red List, based on criterion D. We recommend that the remnant population and its habitat need urgent protection and monitoring and that ex situ conservation, for future reintroduction, should be implemented.

A commentary on the highlights of the 2013 EUCARPIA (the European Associationc for Research on Plant Breeding) Plant Genetic Resources Section meeting at the Swedish University of Agricultural Sciences in Alnarp is presented here. The central theme of the meeting was the use of plant genetic resources in pre-breeding programmes, particularly in the context of incipient climate change and human population pressure.