Field experiments were conducted in 1992 and 1993 to determine the timing and amount of rainfall required to activate UCC-C4243 applied preemergence. UCC-C4243 at 0, 70, and 140 g ai ha−1 was applied 1, 7, 14, and 21 d before 0.5 or 2 cm of simulated rainfall. Temporary rainshelters protected field plots from natural rainfall during the 21 d dry period. Herbicide activity was determined in the field by seeding lentil, wheat, common lambsquarters, and field pennycress and in the greenhouse by a sugarbeet bioassay of soil samples (0 to 3 cm depth) taken from all plots immediately before irrigation. UCC-C4243 did not injure wheat; however, lentil population was reduced when simulated rainfall occurred within 7 d after application. Lentil injury was greater with higher herbicide rate and higher water level. UCC-C4243 at 70 and 140 g ha−1 reduced populations of both weed species by 75 and 90%, respectively, when either 0.5 or 2 cm simulated rainfall was received within 1 d after herbicide application. Weed control was reduced with a 21 d delay between herbicide application and water activation. The sugarbeet bioassay showed a linear decrease of herbicide activity over time and also with accumulated photosynthetically active radiation. After 17.9 d, herbicide activity on a dry soil surface decreased 50%. Laboratory investigations show that [14C]-UCC-C4243 on glass slides was photodegraded by near ultraviolet light (290 to 400 nm). Volatilization of 14C-labeled herbicide from glass slides was less than 5% after exposure to turbulent air for 48 h.

The effects of 14 herbicide treatments, tested in the establishment phase, were investigated on timothy productivity in two field experiments conducted at Melfort, Saskatchewan. Satisfactory weed control achieved with chlorsulfuron, metsulfuron, thifensulfuron, bentazon, diclofop plus bromoxynil, and MCPA/mecoprop/dicamba during the establishment year ensured good forage establishment which in turn caused a ‘residual effect’ for 1 to 3 yr increasing individual cut as well as cumulative forage dry matter yields significantly. In the stand established in 1985, when field pennycress (97 m-2) was the dominant weed in the seedling stage, these treatments increased yields 29 to 52% over a 3-yr period. In the stand established in 1986, when common lambsquarters (27 m-2) was the dominant weed, these treatments increased cumulative yields 24 to 44%. Sethoxydim and fenoxaprop, tested alone, caused significant crop injury in both experiments.

The ability of Medicago sativa to suppress weed growth may provide a viable alternative to chemical weed control and allow crop producers to reduce herbicide inputs. Quantitative information regarding the suppressive effect of M. sativa on weed populations in current cropping systems is lacking. A survey was conducted in Manitoba, Canada, in 1993 and 1994 to investigate weed populations in commercial cereal fields that had been preceded by either M. sativa hay or cereal grain crops. A total of 117 fields were surveyed; approximately half from each field type. Principle component analysis indicated that the inclusion of M. sativa in crop rotations resulted in weed communities different from those of continuous cereal fields. Naturally occurring populations of Avena fatua, Cirsium arvense, Brassica kaber, and Galium aparine were lower in cereal fields that had previously contained M. sativa than in cereal fields that had been preceded by a cereal crop. Lower field uniformity values for C. arvense and Avena fatua indicated that these weeds were also more patchy in the M. sativa rotations. Population differences between field types were nonsignificant for Amaranthus retroflexus, Chenopodium album, and Polygonum convolvulus, and although populations of Taraxacum officinale and Thlaspi arvense were greater in M. sativa/cereal fields than in continuous cereal crops. No consistent effect of field type on Setaria viridis populations was observed. These results show that M. sativa effectively suppressed some, but not all, of the weeds found in the study area. Including M. sativa hay crops in crop rotations can be part of an integrated weed management strategy for weeds such as A. fatua, B. kaber, and C. arvense.

Phytotoxic effects of metabolites from a naturally occurring rhizobacterial isolate, Pseudomonas syringae strain 3366, were determined on downy brome and ‘Hill 81’ winter wheat, along with 10 other weed and crop species. Centrifuged supernatant and concentrated ethyl acetate extracts from aerobic shake cultures of strain 3366 suppressed germination of seeds and reduced root and shoot growth in agar diffusion assays, soil assays, and under field conditions. Generally, root growth was inhibited more than shoot growth. Strain 3366 metabolites applied in soil inhibited all species tested. Crude ethyl acetate extracts in soil inhibited downy brome at concentrations that had little effect on winter wheat. Inhibitory activity was greater in Palouse silt loam (pH 5.8, 3.6% organic matter) than in Shano silt loam (pH 9.0, 0.8% organic matter). Activity of extracted metabolites decreased rapidly in wet soil but remained high in dry soil. Active metabolites were isolated and purified from the ethyl acetate extract using column chromatography, thin-layer chromatography, and crystallization. Chemical analysis revealed the presence of phenazine-1-carboxylic acid, 2-amino phenoxazone, and 2-amino phenol. Activity of these metabolites against downy brome was confirmed in agar assays. Phenazine-1-carboxylic acid, the major identifiable metabolite present in ethyl acetate extracts (20% by weight), inhibited downy brome root growth by 99% at concentrations of 5.7 mg L−1. Production of these metabolites in field soil by live bacteria of strain 3366 was confirmed with thin-layer chromatography.

Studies were conducted to determine the effect of ensiling and/or rumen digestion by cattle on the germination and viability of several common weed species. Seed survival of grass species subjected to ensiling and/or rumen digestion tended to be less than that of broadleaf species. Downy brome, foxtail barley, and barnyardgrass were nonviable after either ensiling for 8 weeks or rumen digestion for 24 h. Some green foxtail (17%) and wild oats (0 to 88%) seeds survived digestion in the rumen but were killed by the ensiling process. Varying percentages of seeds of kochia, redroot pigweed, common lambsquarters, wild buckwheat, round-leaved mallow, and field pennycress remained viable after ensiling (3 to 30%), rumen digestion (15 to 98%), and ensiling plus rumen digestion (2 to 19%). A time course study of rumen digestion indicated that loss of seed viability often was not a gradual process. With some species, there was an initial lag phase while degradation of the protective seed coat likely occurred, followed by a rapid decline in embryo viability. The diet fed to livestock appeared to affect viability losses caused by rumen digestion. Estimates of seed survival with varying rates of passage through the rumen due to differing ratios of grain to forage in the diet are presented.

Field studies were conducted to determine suitability of flurtamone alone and in tank mixture with ethalfluralin for selective control of weeds in safflower. Flurtamone at 0.6 to 0.9 kg ha−1 controlled field pennycress, kochia, and Russian thistle. Flurtamone and ethalfluralin were compatible in tank mixture and provided comparable or superior control of wild oat, kochia, and field pennycress than either herbicide alone. Plant height, date of flowering, seed yield, and quality data indicated that safflower tolerated flurtamone alone up to 1.6 kg ha−1 and flurtamone tank mixed with ethalfluralin at rates as high as 1.1 plus 1.1 kg tar1, respectively. Safflower yield response to these herbicides was positive and reflected level of weed control attained with various treatments.

Chlorsulfuron at 0.018 kg ai/ha, metsulfuron at 0.0045 kg ai/ha, and 2,4-D at 1.1 kg ae/ha plus dicamba at 0.28 kg ae/ha were applied over snow-covered winter wheat to study the feasibility of combining herbicides with a graphite snow-removal treatment. Chlorsulfuron or metsulfuron applied on snow effectively controlled annual broadleaf weeds when tank mixed with urea ammonium nitrate (UAN) at 28 kg N/ha and graphite powder at 20 kg/ha. Over-snow treatments of 2,4-D plus dicamba plus UAN did not control weeds. There was no antagonism or synergism among herbicides, UAN, or graphite.

A long-term study was conducted at Lethbridge, Alberta, to determine the response of weed populations to various crop rotations and tillage treatments. Weed density and species composition differed with rotation, tillage, and date of sampling within years. Fewer weeds were found in winter wheat-fallow than continuous winter wheat, winter wheat-lentil, or winter wheat-canola rotations. A dense infestation of downy brome developed in the continuous winter wheat rotation. In all rotations, more weeds were present in zero tillage plots than in either minimum or conventional tillage plots. Dandelion and perennial sowthistle densities increased slightly over years in the minimum and zero tillage treatments. Flixweed, field pennycress, wild buckwheat, and common lambsquarters densities decreased in zero till but densities of downy brome, redroot pigweed, and Russian thistle increased. Russian thistle was not well controlled with trifluralin and its density increased in the winter wheat-canola rotation.

Field experiments were initiated at Alliance and Hairy Hill, Alberta, in 1989 to investigate the effects of conventional tillage, zero tillage, and four levels of nitrogen fertilizer on continuous barley production. In both tillage systems, the nitrogen was banded 6 to 8 cm deep between alternate barley rows. Herbicides were used for weed control each year. The influence of tillage and nitrogen on weed seed population dynamics was determined in 1991 and 1992. In the zero-tillage system, a large proportion of the weed seeds were present either at the soil surface or at the 5- to 10-cm depth. Green foxtail, the dominant species at Alliance, was also present at Hairy Hill where field pennycress was dominant. Green foxtail was consistently associated with low (residual) nitrogen and, in most cases, with conventional tillage. At both locations, green foxtail populations tended to decrease to very low levels as nitrogen rate increased, especially in zero tillage. At Hairy Hill, field pennycress populations in the soil seedbank were higher in zero tillage compared with conventional tillage, but plants that emerged from the soil seedbank in the field in spring were lower in zero tillage. Field pennycress populations were highest under low nitrogen. The results indicate that banding nitrogen has the potential to be an effective tool for green foxtail and field pennycress management in conventional- and zero-tillage systems, resulting in less dependence on herbicides for their control.

Laboratory and greenhouse studies were conducted to determine the mode of action of soil- and foliar-applied UCC-C4243. Experiments demonstrated that UCC-C4243 required light for phytotoxicity, phytotoxic symptoms were similar to inhibitors of porphyrin synthesis such as acifluorfen, and UCC-C4243 potently inhibited protoporphyrinogen oxidase. Germination and emergence of field pennycress and lentil in the dark were not affected by soil-incorporated UCC-C4243 at rates more than 10 times greater than like treatments that killed all plants in the light. Soil-incorporated UCC-C4243 required light for activity and killed seedlings within 1 d after emergence; sublethal doses caused desiccation, veinal necrosis, and leaf deformation. Field pennycress and lentil were susceptible to soil-incorporated UCC-C4243 and acifluorfen in the light, but were 5 to 93 times less sensitive to the herbicides in the dark. Wheat was not affected by either herbicide in the light or dark. Injury symptoms from UCC-C4243 applied POST to redroot pigweed were similar to symptoms from diphenyl ether and bipyridinium herbicides: rapid, light-dependent chlorophyll bleaching, desiccation, and necrosis. UCC-C4243, acifluorfen-methyl, and acifluorfen acid caused light- and concentration-dependent chlorophyll bleaching and electrolyte leakage from cucumber leaf disks (I50 = 1.0, 1.8, and 4.3 μM, respectively). An inhibitor of the porphyrin synthesis pathway, 4,6-dioxoheptanoic acid, almost completely inhibited herbicide-induced electrolyte leakage. δ-Aminolevulinic acid, a tetrapyrrole precursor and stimulator of the porphyrin synthesis pathway, caused synergistic effects with each herbicide. Protoporphyrinogen oxidase from barley etioplast preparations was inhibited 50% by 40 nM UCC-C4243. Barley leaf sections treated with 100 μM UCC-C4243 accumulated protoporphyrin IX in vivo to levels > 75 times non-treated controls. These data indicate the light-requiring herbicide activity of UCC-C4243, like acifluorfen, is due to inhibition of protoporphyrinogen oxidase.

Dicamba and 2,4-D, which are used prior to seeding in conservation tillage systems, were applied in late fall and early spring to determine their residual effect on subsequent spring-seeded crops. The herbicides 2,4-D ester and 2,4-D amine with dicamba or glyphosate, applied 0 or 15 d prior to spring seeding, damaged canola, peas, lentils, and alfalfa. The legumes were damaged by spring-applied dicamba. There was also a slight reduction in the total dry matter yield of wheat and barley by 2,4-D applied in spring prior to seeding. All crops tested, except lentils, were tolerant of recommended fall applications of 2,4-D for the control of winter annual flixweed and field pennycress.

Effects of ethylene, gibberellins, kinetin, nitrate, light, and temperature, each alone or combined with other factors, were studied on the germination of dormant seed of field pennycress (Thlaspi arvense L. # THLAR). Treated seed were incubated at a range of constant temperatures, at temperatures alternating daily between 12 and 22 C (12 h each), or at 12 C followed by transfer to 22 C. No germination ocurrred in water under any conditions. When applied in the dark KNO3 had no effect on germination under any of the temperature regimes. A combination of KNO3, light, and a shift in temperature from 12 to 22 C induced 100% germination. Four days of incubation at 12 C induced maximal seed sensitivity towards KNO3 + light, and the sensitivity remained unchanged if the incubation at 12 C was extended to 15 days. Upon transfer to 22 C, the effect of low-temperature incubation was lost within 3 days. Of the two seed lots studied, one had a relatively lower chilling requirement, but both had similar requirements for light and KNO3. Gibberellic acids4+7 (GA4+7) induced 100% germination in the dark or light at all temperature regimes. Gibberellic acid3 (GA3) was promotive under isothermal conditions, but its effectiveness was enhanced by a shift in temperature from 12 to 22 C. Kinetin or ethephon, each applied alone, had little effect on germination. Ethephon and GA3 together induced 100% germination.

14C-chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino] carbonyl] benzenesulfonamide} and its metabolites were extracted from flax (Linum sativum L.), field penny cress (stinkweed) (Thlaspi arvense L. # THLAR), and wheat (Triticum aestivum L.) with an average efficiency of 94% using an aqueous extraction procedure. Chlorsulfuron and its metabolites were separated on a reverse-phase preparative chromatography column by eluting with a step gradient of aqueous 0.1% (v/v) formic acid and methanol. Major peaks were eluted at 35 and 45% methanol, and minor peaks at 10, 25, and 100% methanol. The 45% methanol peak represented unmetabolized chlorsulfuron or its hydroxylated derivative and was the only fraction that exhibited biological activity. In wheat and flax, 75 and 62%, respectively, of the extracted activity were eluted by 35% methanol and, in the case of wheat, this fraction was shown to be a glycosylated derivative of chlorsulfuron. Although 14C-chlorsulfuron was metabolized to a lesser extent in field pennycress, our data indicate that over 50% of the absorbed 14C-activity was recovered in forms other than 14C-chlorsulfuron 5 days after treatment. The separation procedure can be used readily to assess the amount of chlorsulfuron detoxification that occurs in plants.

Generalizations concerning the effects of management practices on weed community dynamics often lack robustness, most likely because of the concomitant effects of agronomic and environmental factors. However, such generalizations, when valid, provide useful grounds for predictions and are thus desirable. This study attempted to evaluate the relative importance of crop rotation, tillage, and weed management as factors affecting weed communities and tested the hypothesis of an association between management practices and weeds from certain life cycle groups. Principal component analysis (PCA) of weed density data from a 4-yr field study conducted on a Kamouraska clay and a Saint-André gravelly sandy loam at La Pocatière QC, Canada, identified groups of weed species, while an analysis of variance (ANOVA) of PCA scores associated these groups with management factors. A multivariate analysis of variance (MANOVA) of regression coefficients describing time courses of density for each species confirmed treatment effects. Species segregated roughly according to life cycles. Interactions among weed management intensity, tillage, and crop rotation mostly explained species dominance in the various cropping systems. A first group of species, mostly annual dicots, largely dominated in minimum weed management treatments; their relative importance in each rotation varied with their level of susceptibility to postemergence herbicides. A second group included annuals and perennials, whose commonality seemed to be their tolerance to herbicides; these species also had a particular affinity for chisel and no-till treatments. A third group was formed by perennial species, each with a different response to tillage. The tenuous correspondence between commonly used classification schemes and management factors suggests that other aspects of weed biology (e.g., seed size, dispersal, production, germination requirements, and seedbank longevity) should be considered when trying to explain and predict the presence and dominance of certain weed species with regard to management practices.

In greenhouse experiments, Ohio accessions of 22 weed species representing 13 dicot families were screened as alternative hosts of soybean cyst nematode (SCN, Heterodera glycines). Purple deadnettle (Lamium purpureum), henbit (Lamium amplexicaule), field pennycress (Thlaspi arvense), shepherd's-purse (Capsella bursa-pastoris), and a susceptible soybean (Glycine max) cultivar produced SCN population densities of 510, 155, 73, 1, and 366 cysts/450 cm3 soil, respectively, 5 wk after inoculation with eggs from a race 3 SCN population. Purple deadnettle was also a strong host of race 1 SCN and a weak host of race 6 SCN. Average numbers of eggs/cyst among race 3 hosts were highest in purple deadnettle (357), followed by soybean (292), field pennycress (266), henbit (122), and shepherd's-purse (none detected). To our knowledge, henbit is the only SCN host identified here that has been previously identified as a host. The weeds identified as SCN hosts in this study have a winter annual life cycle in Ohio and may serve as sites for SCN reproduction in infested fields during the early or late growing season and when soybean plants are absent.