Certified Crop Advisors of Arkansas and members of the Arkansas Crop Consultants Association were surveyed in Fall 2006 through direct mail to assess current weed management practices and needs in cotton from both a research and educational perspective. Consultants reported scouting 162,300 of the possible 473,700 ha of cotton grown in Arkansas. Collectively, glyphosate-resistant and enhanced glyphosate-resistant cultivars were reported grown on 98% of the cotton hectares. Ninety-five percent of the consultants believe the planting of enhanced glyphosate-resistant cultivars will increase over the next 5 yr. All consultants indicated a “moderate” to “high” level of concern with herbicide-resistant weeds in cotton, and 79% of the consultants suspect herbicide resistance in the fields they scout, predominately glyphosate-resistant horseweed. Horseweed, Palmer amaranth, and morningglories were the three most problematic weeds in cotton. A continued focus on resistant weed management was the most frequent research and educational request by consultants.

Field experiments were conducted in Georgia to evaluate weed control and crop tolerance with glufosinate applied to ‘PHY 485 WRF®’ cotton. This glyphosate-resistant cotton also contains a gene, used as a selectable marker, for glufosinate resistance. Three experiments were maintained weed-free and focused on crop tolerance; a fourth experiment focused on control of pitted morningglory and glyphosate-resistant Palmer amaranth. In two experiments, PHY 485 WRF cotton was visibly injured 15 and 20% or less by glufosinate ammonium salt at 430 and 860 g ae/ha, respectively, applied POST two or three times. In a third experiment, glufosinate at 550 g/ha injured cotton up to 36%. Pyrithiobac or glyphosate mixed with glufosinate did not increase injury compared to glufosinate applied alone; S-metolachlor mixed with glufosinate increased injury by six to seven percentage points. Cotton injury was not detectable 14 to 21 d after glufosinate application, and cotton yields were not reduced by glufosinate or glufosinate mixtures. A program of pendimethalin PRE, glyphosate applied POST twice, and diuron plus MSMA POST-directed controlled glyphosate-resistant Palmer amaranth only 17% late in the season. S-metolachlor included with the initial glyphosate application did not increase control, and pyrithiobac increased late-season control by only 13 percentage points. Palmer amaranth was controlled 90% or more when glufosinate replaced glyphosate in the aforementioned system. Pitted morningglory was controlled 99% by all glufosinate programs and mixtures of glyphosate plus pyrithiobac. Seed cotton yields with glufosinate-based systems were at least 3.3 times greater than yields with glyphosate-based systems because of differences in control of glyphosate-resistant Palmer amaranth.

Four field experiments were conducted in Louisiana and Mississippi in 2009 and 2010 to evaluate POST herbicides treatments with tembotrione applied alone or as a prepackaged mixture with thiencarbazone for weed control in corn. Treatments included tembotrione at 92 g ai ha−1, thiencarbazone : tembotrione at 15 : 76 g ai ha−1, atrazine at 2,240 g ai ha−1, glufosinate at 450 g ai ha−1, glyphosate at 860 g ae ha−1, and coapplications of tembotrione or thiencarbazone : tembotrione with atrazine, glufosinate, or glyphosate. All treatments were applied to 26-cm corn in the V4 growth stage. Treatments containing thiencarbazone : tembotrione and those with tembotrione controlled barnyardgrass, browntop millet, entireleaf morningglory, hophornbeam copperleaf, johnsongrass, Palmer amaranth, and velvetleaf 85 to 96% and 43 to 97% 28 d after treatment and at corn harvest, respectively. Corn yield ranged from 9,200 to 10,420 kg ha−1 and was greater than the nontreated control following all herbicide treatments, except atrazine alone. Results indicated that thiencarbazone : tembotrione or tembotrione POST is an option for weed management in corn, and applications of thiencarbazone : tembotrione would be strongly encouraged where rhizomatous johnsongrass is problematic.

A study was conducted near Garden City, KS, under irrigated conditions to determine the effect of full-season Palmer amaranth infestation on corn water use efficiency and light interception in a fully developed corn canopy. Palmer amaranth at densities of 0, 0.5, 1, 2, 4, and 8 plants m−1 was established at corn planting in 1996 and 1997 and at two locations in 1998. Soil water was monitored 240 cm deep in 30-cm increments with a neutron probe each year and at each location every 10 d. Photosynthetic photon flux was measured in 1997 and 1998 by using a circular and a linear quantum sensor for above canopy and in four 50-cm increments for within canopy, respectively. Palmer amaranth reduced corn yield from 11 to 91% as density increased from 0.5 to 8 plants m−1. Water use efficiency of corn declined with increased Palmer amaranth density. Regardless of Palmer amaranth density, soil water extraction was greatest in the top 30 cm of the soil profile. The pattern of corn leaf area distribution was similar across Palmer amaranth densities, with 15, 70 to 75, and 5 to 15% of the total leaf area occurring 1.5 m, 0.5 to 1.5 m, and 0 to 0.5 m above the ground, respectively. In weed-free corn, over 60% of light was intercepted from 0.5 to 1.5 m above the ground. In contrast, in mixed canopies 60 to 80% of light was intercepted 1 m above the ground, where 80% of Palmer amaranth leaf area was concentrated. Under the conditions of this study, water was not a limiting factor. The effect of Palmer amaranth density on total light interception was not significant. However, within each treatment, light interception at different heights differed, emphasizing the importance of evaluating the vertical distribution of light through the canopy to assess the effect of weed height on light competition.

Research was conducted for 2 yr at Marianna, AR, to determine whether the fall-planted cover crops rye, wheat, turnip, and a blend of brown and white mustard (Caliente) would aid weed management programs in conservation-tilled, enhanced, glyphosate-resistant cotton. Wheat and rye easily were established both years and turnip and mustard blend stands were better in the second year. The cover crops alone were more suppressive of Palmer amaranth, pitted morningglory, and goosegrass in 2007 than in 2008. Rye was generally superior to wheat in suppressing the three evaluated weeds. Once herbicides were applied, there were seldom differences among cover crops for a particular herbicide program as a result of the highly efficacious herbicide programs. Cotton yields were not affected by wheat, rye, or the mustard blend, but yields were lowest in plots that followed turnip both years, possibly because of allelopathy. Integration of cover crops, especially cereals, into conservation-tilled, glyphosate-resistant cotton aided early-season weed management and could reduce the selection of glyphosate for herbicide resistance.

Waterhemp is an increasingly problematic weed in the U.S. Midwest, having now evolved resistances to herbicides from six different site-of-action groups. Glyphosate-resistant waterhemp in the Midwest is especially concerning given the economic importance of glyphosate in corn and soybean production. Amplification of the target-site gene, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was found to be the mechanism of glyphosate resistance in Palmer amaranth, a species closely related to waterhemp. Here, the relationship between glyphosate resistance and EPSPS gene amplification in waterhemp was investigated. Glyphosate dose response studies were performed at field sites with glyphosate-resistant waterhemp in Illinois, Kansas, Kentucky, Missouri, and Nebraska, and relative EPSPS copy number of survivors was determined via quantitative real-time polymerase chain reaction (qPCR). Waterhemp control increased with increasing glyphosate rate at all locations, but no population was completely controlled even at the highest rate (3,360 g ae ha−1). EPSPS gene amplification was present in plants from four of five locations (Illinois, Kansas, Missouri, and Nebraska) and the proportion of plants with elevated copy number was generally higher in survivors from glyphosate-treated plots than in plants from the untreated control plots. Copy number magnitude varied by site, but an overall trend of increasing copy number with increasing rate was observed in populations with gene amplification, suggesting that waterhemp plants with more EPSPS copies are more resistant. Survivors from the Kentucky population did not have elevated EPSPS copy number. Instead, resistance in this population was attributed to the EPSPS Pro106Ser mutation. Results herein show a quantitative relationship between glyphosate resistance and EPSPS gene amplification in some waterhemp populations, while highlighting that other mechanisms also confer glyphosate resistance in waterhemp.

A simulation model is used to explore management options to mitigate risks of glyphosate resistance evolution in Palmer amaranth in glyphosate-resistant cotton in the southern United States. Our first analysis compares risks of glyphosate resistance evolution for seven weed-management strategies in continuous glyphosate-resistant cotton monoculture. In the “worst-case scenario” with five applications of glyphosate each year and no other herbicides applied, evolution of glyphosate resistance was predicted in 74% of simulated populations. In other strategies, glyphosate was applied with various combinations of preplant, PRE, and POST residual herbicides. The most effective strategy included four glyphosate applications with a preplant fomesafen application, and POST tank mixtures of glyphosate plus S-metolachlor followed by glyphosate plus flumioxazin. This strategy reduced the resistance risk to 12% of populations. A second series of simulations compared strategies where glyphosate-resistant cotton was grown in one-to-one rotations with corn or cotton with other herbicide resistance traits. In general, crop rotation reduced risks of resistance by approximately 50% and delayed the evolution of resistance by 2 to 3 yr. These analyses demonstrate that risks of glyphosate resistance evolution in Palmer amaranth can be reduced by reducing glyphosate use within and among years, controlling populations with diverse herbicide modes of action, and ensuring that population size is kept low. However, no strategy completely eliminated the risk of glyphosate resistance.

Field studies were conducted near Clayton, Goldsboro, Kinston, and Rocky Mount, NC in 2003 to evaluate weed control and cotton response to postemergence (POST) treatments of glufosinate applied alone or in tank mixtures with s-metolachlor, pyrithiobac, or trifloxysulfuron. Late-season control of common lambsquarters, common ragweed, entireleaf morningglory, ivyleaf morningglory, jimsonweed, pitted morningglory, purple nutsedge, and sicklepod with glufosinate early postemergence (EPOST) was ≥90%. The addition of S-metolachlor to glufosinate EPOST improved control of all weeds except sicklepod, ivyleaf morningglory, and entireleaf morningglory. When applied POST, glufosinate provided ≥90% late season control of common lambsquarters, common ragweed, entireleaf morningglory, ivyleaf morningglory, jimsonweed, large crabgrass, pitted morningglory, purple nutsedge, and sicklepod. Control of goosegrass and Palmer amaranth was 81 and 84%, respectively. When pyrithiobac or trifloxysulfuron were added in POST tank mixtures, control of Palmer amaranth improved 6 and 9 percentage points, respectively. Control of goosegrass remained near 80% regardless of herbicide treatment used. The addition of a late post-directed (LAYBY) tank-mixture of glufosinate plus prometryn provided ≥88% late season control of all weeds. Reduced control of goosegrass and Palmer amaranth was observed with the LAYBY tank mixture of glufosinate plus MSMA when compared to other LAYBY tank mixtures. Cotton lint yields in plots receiving any herbicide application were significantly higher than plots receiving no herbicide application for all application timings. Cotton lint yields were ≥ 740 kg/ha where an EPOST was applied and ≥ 680 kg/ha when a POST herbicide was applied. Cotton lint yields were at least 200 kg/ha greater on plots receiving a LAYBY application when compared to plots where no LAYBY treatment was applied.

Palmer amaranth is the most common and troublesome broadleaf weed species of rice in Mississippi because of the effects of early-season interference and infestations on rice levees, and herbicides for residual or POST control of Palmer amaranth in rice are limited. Three studies were conducted in 2012 and 2013 to evaluate application rates and timings of saflufenacil in rice and to determine the influence of adjuvants when mixed with saflufenacil applied POST. In a PRE study, no injury occurred after saflufenacil PRE, and no control was observed from carfentrazone. Hemp sesbania and Palmer amaranth control increased with increasing saflufenacil rate when applied PRE. Hemp sesbania control with saflufenacil at any rate PRE was ≤ 25% at 35 d after treatment (DAT). Palmer amaranth and ivyleaf morningglory control with saflufenacil at 75 g ai ha−1 PRE was ≥ 94% 35 DAT. In a POST study, rice injury was influenced by application timing and rate of saflufenacil; however, efficacy was not. Rice injury with saflufenacil at 25 g ha−1 and carfentrazone early POST (EPOST) and late POST was similar 7 DAT. Saflufenacil at 50 and 75 g ha−1 EPOST were the most injurious 7 DAT. Control of hemp sesbania and ivyleaf morningglory was similar for all rates of saflufenacil and carfentrazone; however, Palmer amaranth control with saflufenacil at any rate was greater than that of carfentrazone 14 and 28 DAT. In an adjuvant study, rice injury was influenced by adjuvant and saflufenacil rate. Saflufenacil applied alone or in mixture with crop oil concentrate (COC) was least injurious, and saflufenacil at 50 g ha−1 was more injurious than saflufenacil at 25 g ha−1. Saflufenacil applied in combination with any adjuvant provided better control of hemp sesbania and Palmer amaranth than saflufenacil alone. On the basis of this research, saflufenacil should be applied PRE at 50 or 75 g ha−1, depending on weed spectrum, and POST applications should be made at 25 g ha−1 in combination with COC after the two-leaf rice growth stage.

Since its discovery in 2005, glyphosate-resistant Palmer amaranth has become a major problem for many farmers in the southern United States. One mechanism of resistance found in a Georgia population of glyphosate-resistant Palmer amaranth is amplification of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene throughout the genome, with some resistant plants containing and expressing more than 100 EPSPS genes. Such high numbers of EPSPS genes and protein production could result in a fitness cost to resistant plants due to (1) metabolic cost of overproduction of this enzyme and (2) disruption of other genes after insertion of the EPSPS gene. A greenhouse experiment was set up to investigate differences in growth and reproduction between glyphosate-susceptible and -resistant Palmer amaranth plants. Measurements included growth rate, plant height/volume ratio, final biomass, photosynthetic rate, inflorescence length, pollen viability, and seed set. This study found no significant fitness costs for plants with the resistance trait. This study also provided a clear example of how controlling for genetic background is important in fitness cost studies and how potentially misleading results can be obtained if only a few fitness traits are measured. These results indicate that glyphosate-resistant Palmer amaranth plants with high EPSPS gene copy numbers are likely to persist in field populations, even in the absence of glyphosate, potentially leading to long-term loss of glyphosate as a control option for Palmer amaranth.

Several inbred lines of acetolactate synthase (ALS)-inhibiting herbicide-resistant (ALS-R) Palmer amaranth and ALS-susceptible (ALS-S) common waterhemp were developed in the greenhouse. Interspecific hybrids were obtained by allowing several ALS-S common waterhemp females to be pollinated only by ALS-R Palmer amaranth in a growth chamber. Putative hybrid progeny were treated with an ALS-inhibiting herbicide, and the hybrid nature verified using a polymorphism found in the parental ALS gene. Polymerase chain reaction (PCR) was used to amplify a region of the ALS gene in both parental plants and putative hybrids. Restriction enzyme digestion of the ALS-R Palmer amaranth PCR fragment resulted in two smaller fragments, whereas the PCR fragment in the ALS-S common waterhemp was not cut. Restriction digestion of the putative hybrid PCR fragment showed a combination of ALS-R Palmer amaranth double fragments and an ALS-S common waterhemp single fragment. Approximately 4 million flowers were present on 11 common waterhemp females and produced about 44,000 seeds that appeared viable. From the approximately 3,500 putative hybrid seedlings that were screened, 35 were confirmed as hybrids using herbicide resistance as a phenotypic and molecular marker. The data collected here verify that interspecific hybridization does occur between these two species, and even at a low rate, it could contribute to the rapid spread of ALS resistance in these species.

The dissipation of trifloxysulfuron in solution has not been evaluated. The objectives of this study were to evaluate the effect of spray carrier pH (pH values of 5, 7, and 9) on dissipation of trifloxysulfuron during a 21-d period and to evaluate the resulting effects on efficacy on Palmer amaranth. ANOVA of half-life values indicated that hydrolysis occurred more rapidly under acidic spray carrier pH with no differences between neutral and alkaline pH levels. Trifloxysulfuron solutions that were applied to Palmer amaranth significantly decreased dry weights compared with nontreated at all pH levels and time intervals. These results indicated that trifloxysulfuron dissipation was most rapid when the spray tank pH was more acidic, but trifloxysulfuron efficacy was not adversely affected by chemical degradation on Palmer amaranth. Data suggest that pesticide applicators may reduce trifloxysulfuron degradation in spray tanks by using neutral or alkaline spray carriers.

Research was conducted in Mississippi in 2012 and 2013 to compare the efficacy of saflufenacil to other broadleaf herbicides applied in mixtures with imazethapyr in a Clearfield rice system. Saflufenacil at 50 g ai ha−1, carfentrazone at 35 g ai ha−1, a prepackaged mixture of halosulfuron plus thifensulfuron at 35 plus 4 g ai ha−1, and a prepackaged mixture of propanil plus thiobencarb at 2,240 plus 2,240 g ai ha−1 were applied in mixture with imazethapyr at 70 g ai ha−1 early-POST (EPOST) to rice in the one- to two-leaf stage or late-POST (LPOST) to rice in the four-leaf to one-tiller stage. No differences in injury among the broadleaf herbicides or between application timings were detected at any evaluation. Imazethapyr combined with propanil plus thiobencarb or saflufenacil provided the greatest control of barnyardgrass 7 and 14 d after treatment (DAT). Hemp sesbania, ivyleaf morningglory, and Palmer amaranth control was greatest and similar for imazethapyr combined with carfentrazone, propanil plus thiobencarb, and saflufenacil; however, rough rice yield was greatest for imazethapyr combined with propanil plus thiobencarb or saflufenacil. Propanil plus thiobencarb or saflufenacil can be used in a Clearfield rice system to achieve optimum weed control and highest rice yields.

Cotton gins in Arkansas, western Tennessee, and western Mississippi were sampled for weed seed contamination of gin trash in fall 2007. A total of 473 samples were collected, with 453 samples from Arkansas. The objectives of this research were to determine the weed species most frequently found in gin trash and determine what means gin operators are using to dispose of gin trash. There were 25 weed species found in the gin trash samples—11 grass and 14 broadleaf weeds. Grass and broadleaf weeds were present in 41.4 and 8.5% of the samples, respectively. The most frequently found species were large crabgrass (19.0%), barnyardgrass (14.0%), goosegrass (12.9%), red sprangletop (8.2%) and Palmer amaranth (4.2%). Viable seeds of barnyardgrass, large crabgrass, Palmer amaranth, and prickly sida were present in the surface layer (0- to 25-cm depth) of gin trash piles after 1 yr of composting. Viable Palmer amaranth seeds were present in the surface layer of gin trash piles after 2 yr of composting, but no germinable seeds were found deeper than 25 cm following 1 yr of composting. Gin trash disposal involved application of the material to crop fields during the fall or winter months; composting followed by application of the compost as mulch or a soil amendment to gardens, flower beds, or crop fields; use as cattle feed; and coverage for landfills to reduce erosion and encourage growth of vegetation. Because of the demonstrated potential for weed seed dispersal via gin trash, including composted material, development of technologies to ensure disposal of a gin-trash product free of viable weed seed are justified.

Research was conducted at Marianna, AR, for 2 yr to determine whether hairy vetch and Austrian winter pea cover crops would aid weed management programs in conservation-tilled, enhanced glyphosate-resistant cotton. Both cover crops were easily established and produced rapid growth in early spring, with biomass production of 435 to 491 g m−2 by Austrian winter pea and 415 to 438 g m−2 by hairy vetch. The effect of cover crops on weed control was short-lived in both years, with herbicide programs being the major determinant of weed control and seed-cotton yield. Averaged over cover crops, seed-cotton yields when the initial in-crop glyphosate application was delayed to the four-node cotton stage were up to 710 kg ha−1 less than in a PRE herbicide program. In 1 of 2 yr, seed-cotton yields were greater in PRE-treated plots compared with a program where initial weed management was delayed to the one-leaf stage of cotton. As a result of rapid decay of hairy vetch and Austrian winter pea biomass following cotton planting and the lack of adequate Palmer amaranth, pitted morningglory, and goosegrass control in the absence of herbicides, it appears there may be minimal weed management benefits from the use of hairy vetch and Austrian winter pea in Midsouth cotton production.