Variation in Gremmeniella abietina var.
abietina was studied in three stands of Scots
pine in northern Finland and in the Kola
Peninsula. Eighty-four isolates of large- and small-tree
types of G. abietina var. abietina
(LTT and STT, respectively) were identified
on the basis of tentative characteristics (spore morphology,
disease type and host size), fatty acid and sterol profiles
(FAST), and random amplified microsatellite technique (RAMS).
Both LTT and STT occurred in all three stands. In general,
the classifications
obtained using the three methods agreed with one another,
although a few contradicting results were observed. Variation
in fatty acids and sterols in G. abietina var.
abietina was rather low, although the amounts of
some individual extractives showed statistically
significant differences between the stands. All pathogenic
and asymptotic G. abietina var. abietina
isolates originating from branches
located at heights above the annual snow cover were identified
as LTT based on RAMS, but some were grouped to STT according
to their FAST profiles. Both STT and LTT were detected among
the isolates obtained from seedlings according to both FAST and
RAMS. In addition, in two cases RAMS markers thought to be
STT- or LTT-specific were found in the same isolate. The results
presented here suggest that LTT of G. abietina
var. abietina caused the devastating epidemics on
pines in the first-thinning stage or
middle age similar to pines in this study reported in
northern Finland and in the Kola Peninsula during the 1980s.