Five taxa of the genera Omphalodina and Rhizoplaca known from continental Antarctica are reassessed in order to identify a remarkable species collected from northern Victoria Land, for which the new combination Rhizoplaca macleanii (C.W. Dodge) Castello is proposed here. This poorly known species is known only from continental Antarctica. Two synonyms are discussed: Omphalodina exsulans (Th. Fr.) C. W. Dodge and O. siplei (C. W. Dodge & G. E. Baker) C. W. Dodge are synonyms of Rhizoplaca melanophthalma (DC.) Leuckert & Poelt. The correct name of another species is Tephromela priestleyi (C. W. Dodge) Øvstedal. The name O. johnstonii (C. W. Dodge) C. W. Dodge should be abandoned, type material being too scanty for a reliable identification. A contribution to the flora of the Terra Nova Bay area (northern Victoria Land) is provided, with two additional species, including Buellia vilis Th. Fr. new to Antarctic regions, and two nomenclatural corrections.

The ecologically important lichen Cladonia stellaris forms thick carpets in boreal forest floors. In addition to affecting temperature and water conditions in the soil underneath, the secondary metabolites formed by the lichen layer are of ecological interest. In this paper, we investigated the distribution of lichen acids in C. stellaris collected at different latitudes in Finland and developed methods to quantify the two optical enantiomers of usnic acid separately. The lichen extracts were analysed by high-performance liquid chromatography (HPLC) with UV and mass spectrometric (MS) detection and by gas chromatography with flame ionization (GC-FID) and MS detection. Usnic acid and perlatolic acid were quantified using GC-FID. The concentration of usnic acid in the top 20 mm of the lichen thallus ranged from 0·48–3·08% of dry weight, and that of perlatolic acid from 0·08–0·54%. The enantiomeric composition of usnic acid was determined using a chiral HPLC column coupled to an electrospray ionization-tandem mass spectrometer. (−)-Usnic acid was found to be the predominating enantiomer in all extracts; the proportion of (+)-usnic acid ranged from 0·4%–10·0%. Olivetoric acid methyl ester, diphenylmethanol, and 5-pentylresorcinol were identified, and several other olivetoric acid-type compounds were tentatively identified in the extracts.

This study of elevation gradients of lichen species richness in Nepal aimed to compare distribution patterns of different life-forms, substratum affinities, photobiont types, and Nepalese endemism. Distribution patterns of lichens were compared with elevational patterns shown by a wide range of taxonomic groups of plants along the Nepalese Himalayan elevational gradient between 200–7400m. We used published data on the elevation records of 525 Nepalese lichen species to interpolate presence between the maximum and minimum recorded elevations, thereby giving estimates of lichen species richness at each 100-m elevational band. The observed patterns were compared with previously published patterns for other taxonomic groups. The total number of lichens as well as the number of endemic species (55 spp.) showed humped relationships with elevation. Their highest richness was observed between 3100–3400 and 4000–4100m, respectively. Almost 33% of the total lichens and 53% of the endemic species occurred above the treeline (>4300m). Non-endemic richness had the same response as the total richness. All growth forms showed a unimodal relationship of richness with elevation, with crustose lichens having a peak at higher elevations (4100–4200m) than fruticose and foliose lichens. Algal and cyanobacterial lichen richness, as well as corticolous lichen richness, all exhibited unimodal patterns, whereas saxicolous and terricolous lichen richness exhibited slightly bimodal relationships with elevation. The highest lichen richness at mid altitudes concurred with the highest diversity of ecological niches in terms of spatial heterogeneity in rainfall, temperature, cloud formation, as well as high phorophyte abundance and diversity implying large variation in bark roughness, moisture retention capacity, and pH. The slightly bimodal distributions of saxicolous and terricolous lichens were depressed at the elevational maximum of corticolous lichens.

Buellia nordinii, a new muscicolous or lignicolous species characterized by a blastidiate thallus containing atranorin, apothecia with a fine granular surface, markedly enlarged paraphyses tips and large triseptate ascospores is described from Venezuela. The species grows in very humid situations near the ground in open paramo vegetation at high elevations. It is compared with other known asexually reproducing and triseptate Buellia taxa.

The genetic diversity within and among populations of Xanthoria parietina was studied at the subspecific level with a fingerprinting technique (RAPD-PCR) applied to sterile cultured multispore isolates, each being derived from a single apothecium. Populations from coastal, rural and urban sites from NW, SW and central France and from NE Switzerland were investigated. Between 1 and 8 microsites of a few decimetres square, each comprising 13 to 27 thalli of X. parietina, were analysed per population. A total of 132 isolates from epiphytic and 3 isolates from epilithic specimens were investigated. Phenotypic variation was recorded among some of the thalli in the field and among sterile cultured isolates in the laboratory. A high diversity of genotypes was observed, even among thalli growing side by side in phenotypically homogenous populations. An average of 73·5 % polymorphism was found in all samples. As shown with Principal Coordinates Analysis (PCO), most of the genetic variation (90%) resided within, not among, populations. As X. parietina had previously been shown with molecular and fingerprinting techniques to be homothallic, the potential genetic background of this diversity is discussed. Intense genotype rather than gene (allele) flow seems to be an important element in X. parietina populations.

In this study, types of species in the genus Buellia described by J. Steiner are reviewed. Previously ignored holotypes of B. alboatra var. epipolioides, B. caesioatra, B. leptina, B. procellarum var. continuior, B. skutariensis f. compacta and B. skottsbergii are identified, lectotypes and isolectotypes of B. gotlandica, B. saxorum var. flavescens, B. skutariensis, B. skutariensis f. geographica and B. subtumida are designated, and the presumed holotypes of B. incrustans, B. sardiniensis, B. tergestina, B. trifracta and Diplotomma epipolium var. reagens are corrected to lectotypes. Further, the typification of B. stillingiana is reassessed. Amandinea skottsbergii (J. Steiner & Zahlbr.) Şenkard. and B. flavescens (J. Steiner) Şenkard. are proposed as new combinations. It is argued that the varieties Buellia alboatra var. epipolioides, B. procellarum var. continuior, B. subdisciformis f. compacta (=B. skutariensis f. compacta) and B. subdisciformis f. geographica (=B. skutariensis f. geographica) should no longer be recognized but regarded as synonyms of these species. Buellia leptina is regarded as a synonym of Diploicia subcanescens and B. subtumida is reduced to being a synonym of B. dispersa. Finally, new locations are provided for B. flavescens from Italy and B. sardiniensis from France.

Estimating carbon budgets for poikilohydric organisms, such as lichens and bryophytes, requires methods other than those for homoiohydric plants due to a strong dependency of carbon gain on fluctuating hydration. This paper provides guidance with respect to optimal sampling strategies for estimating annual carbon budgets of lichens and bryophytes, based on a one-year dataset of half-hourly CO2-exchange readings on the epilithic placodioid lichen Lecanora muralis (syn. Protoparmeliopsis muralis) and tests the effects of reduced sampling frequencies and different temporal sampling schemes on carbon budget estimates. Both fine-scale sampling (measurements within a day) and large-scale sampling (selection of days within a year) are addressed.

Lowering the sampling frequency within a day caused large deviations for 24-h (diel) budget estimates. Averaged over a larger number of days, these errors did not necessarily cause a large deviation in the annual budget estimate. However, the occurrence of extreme deviations in diel budgets could strongly offset the annual budget estimate. To avoid this problem, frequent sampling (c. every 1·5 hours) is necessary for estimating annual budgets. For estimating diel budgets and patterns a more frequent sampling (every c. 0·5 hours, balancing data resolution and disturbance) is often needed.

Sampling fewer than 365 days in a given year inevitably caused estimates to deviate from the ‘true’ carbon budget, i.e. the annual budget based on half-hourly measurements during 365 days. Accuracy increased with total sample frequency, and blocking days caused larger deviations than sampling randomly or regularly spaced single days. Restricting sampling to only one season led to strongly biased estimates. The sampling effort required for a reliable estimate of the annual carbon balance of lichens based on simple extrapolations of diel carbon budgets is impracticably large. For example, a relatively large sample of 52 random days yielded an estimate within 25% of the true annual budget with only 60% certainty. Supporting approaches are therefore suggested, in particular extrapolating diel budgets using ‘weather response types’, possibly aided by diel activity patterns from chlorophyll fluorescence, or modelling CO2 exchange as a function of climatic conditions.

The corticolous lichen Lecanora subviridis de la Rosa & Messuti is described from temperate forests in south-western Argentina as new to science. It differs from other species of Lecanora occurring on bark and wood by containing usnic or isousnic acid. The relationships with the other taxa are discussed.

Two asexual reproductive strategies of the common lichen Pseudevernia furfuracea are described. Although the species propagates mainly by isidia, some specimens also show the development of soralia. Morphological, chemical and molecular analyses were performed on three such sorediate specimens from the Canary Islands, Morocco and Turkey. Maximum parsimony, maximum likelihood and Bayesian analyses indicate that: a) sorediate samples represent only a morphological variant of the reproductive mode and b) the separation of taxa (at species level or below) on the basis of their containing either olivetoric acid or physodic and oxyphysodic acids is not appropriate. In addition, a phylogenetic reconstruction of the genus Pseudevernia is presented for the first time. The tree shows two sister monophyletic clades, one containing American species (P. intensa, P. cladonia, P. consocians), and the second encompassing the P. furfuracea samples (including sorediate specimens). The biological and taxonomic significance of soralia in sorediate samples is discussed.

Although the tripartite terricolous lichen Nephroma arcticum is easily accessible to lichen-feeding gastropods, grazing marks are mainly restricted to localized cephalodia with N-fixing Nostoc. We tested if this gastropod preference for cephalodia can be explained by differences in carbon based secondary compounds (CBSCs) in cyanobacterial versus green-algal tissues. CBSCs were non-destructively removed from air-dry thalli by 100% acetone. Compound deficient and control thallus parts were offered to the slug Arion fuscus and grazing preferences were quantified by area measurements in ArcGIS™. The concentrations of CBSCs (phenarctin, usnic acid, nephroarctin and methyl gyrophorate) in thallus parts with and without cephalodia were quantified with HPLC. Compared to purely green-algal parts, cephalodial parts with adjoining fungal tissues contained less defensive compounds, and were preferred by A. fuscus. The cephalodia themselves do not contain any CBSCs. After acetone rinsing, A. fuscus did not discriminate between green-algal and cyanobacterial parts. The results were consistent with the hypothesis that CBSCs in green-algal parts of N. arcticum play a herbivore-defensive role. It is further hypothesized that grazing of cephalodia may lead to N-starvation and reduced growth of N. arcticum thalli in southern portions of its range where lichenivorous gastropods are more abundant. This may play a role in shaping the southern distribution limit of this arctic-boreal lichen species.

Chimeras with green algae are reported for the first time from a lichen outside the Peltigerales, viz. the cyanophilous Lichinomycete Phyllopeltula corticola. The species is reported here to be pantropical, based on additional collections from Hawaii, Puerto Rico, Thailand and the Philippines.

We explored lichen species richness and patterns of lichen succession on rough barked Nothofagus cunninghamii trees and on smooth barked Atherosperma moschatum trees in cool temperate rainforests in Victoria, Australia. Nothofagus cunninghamii trees from the Yarra Ranges, and A. moschatum trees from Errinundra were ranked into size classes (small, medium, large and extra-large), and differences in species richness and composition were compared between size classes for each tree species. Nothofagus cunninghamii supported a rich lichen flora (108 trees, 52 lichen species), with the largest trees supporting a significantly higher number of species, including many uncommon species. This success was attributed to varying bark texture, stand characteristics and microhabitat variations as the trees age. Atherosperma moschatum supported a comparable number of species (120 trees, 54 lichen species). Indeed on average, this host supported more lichen species than N. cunninghamii. However, successional patterns with increasing girth were not as clear for A. moschatum, possibly due to the more stable microclimate that this smooth barked host provided. Victorian cool temperate rainforests exist primarily as small, often isolated pockets within a sea of Eucalypt-dominated, fire-prone forest. Many are regenerating from past disturbance. We find that protection of Victoria's oldest rainforest pockets is crucial, as they represent sources of rare, potentially threatened lichen species, and may be acting as reservoirs for propagules for nearby ageing rainforests. Indeed, even single, large old trees have conservation importance, as they may provide exceptional microhabitats, not found elsewhere in the regenerating rainforest environment.