The goal was to study imprinting effects on birth weight (BW), weaning weight (WW), six months weight (W6), pre- and post-weaning growth rate (GRa and GRb), and pre- and post-weaning Kleiber ratio (KRa and KRb) in Baluchi and Makuie sheep breeds. Analyses were done in a two steps process. In the first step, each trait was analysed with a series of 12 univariate animal models, including different combinations of direct and maternal effects and the best model was selected by the AIC criterion. In the second step, three new models were fitted by adding either maternal imprinting effect, paternal imprinting effect, or both of them, respectively, to the best model already selected in the first step, and changes in AIC were monitored. (Co)variances between traits were estimated using bivariate analyses. In Baluchi sheep, for BW, WW and W6, estimates of maternal imprinting heritability (${\boldsymbol h}_{{\boldsymbol mi}}^2$) were 0.12 ± 0.02, 0.08 ± 0.01 and 0.09 ± 0.01, respectively. In Makuie sheep, paternal imprinting heritability (${\boldsymbol h}_{{\boldsymbol pi}}^2$) for BW, WW, W6, GRb and KRb were 0.17 ± 0.05, 0.8 ± 0.05, 0.38 ± 0.10, 0.71 ± 0.15 and 0.65 ± 0.13, respectively. In Baluchi sheep, strong maternal imprinting correlations were observed between BW and WW (0.93 ± 0.05), BW and W6 (0.80 ± 0.07) and WW and W6 (0.91 ± 0.02). In Makuie sheep, paternal imprinting correlations ranged from −0.97 ± 0.33 (BW-GRb) to 0.99 ± 0.47 (GRb-KRb). It was concluded that imprinting effects need to be included in the statistical model to increase the accuracy of genetic evaluation. However, to have accurate and reliable estimates of imprinting effects, the availability of large data sets and deep pedigrees are necessary.

Records on growth and reproductive characteristics collected from 1993 to 2013 at the Breeding Station of Kermani sheep, south-east of Iran, were used for estimating genetic parameters related to parent-of-origin effects. The studied growth traits were birth weight (BWT), weaning weight (WWT), six-month weight (6MW), average daily gain from birth to weaning (ADG1), and average daily gain from weaning to six months of age (ADG2). The reproductive traits were litter size at birth per ewe lambing (LSB), litter size at weaning per ewe lambing (LSW), the sum of litter weight at birth per ewe lambing (LWB), and the sum of litter weight at weaning per ewe lambing (LWW). Models with and without imprinting (maternal and paternal) effects were compared by applying Akaike's information criterion (AIC). Maternal imprinting effects explained 14, 7, 24 and 5% of the phenotypic variances for BWT, WWT, 6MW and ADG1, respectively and their inclusion in the model decreased direct heritability estimates for BWT (from 0.07 to 0.05), WWT (from 0.29 to 0.23), 6MW (from 0.26 to 0.12) and ADG1 (from 0.20 to 0.17). Imprinting effects did not affect ADG2 and studied reproductive traits. The results indicated that a considerable proportion of the maternal imprinting effects overlap with direct additive genetic and maternal effects. Therefore, imprinting effects may be crucial for the genetic assessment of BWT, WWT, 6MW and ADG1 in Kermani sheep.

The ammonia removal ability of heterotrophic bacteria Alcaligenes faecalis strain No.4 isolated from sewage sludge was examined in a batch operation to mitigate ammonia from piggery wastewater, consequently preventing pollution by the inflow of wastewater from piggeries adjacent to rivers. If this process works functionally, it can be effective in controlling nitrous oxide (N2O) and nitrate (NO3−) emissions derived from animal agriculture, the heterotrophic nitrifying and the aerobic denitrifying effect of A. faecalis strain No.4 on high-strength ammonium (NH4+-N) were evaluated in wastewater collected from a piggery. The removal rate by A. faecalis strain No.4 on high-strength ammonium (NH4+-N) was 0.97 kg N/m3/day which was more than 100 fold greater than that achieved using conventional aerobic nitrification and anaerobic denitrification processes. An aerobic one-step denitrification system using A. faecalis strain No.4 can be proposed to remove ammonia and phytopathogens from piggery wastewater with high efficiency and prevent water pollution in adjacent rivers.

The inoculation with Azospirillum brasilense has the potential to reduce the use of mineral fertilizers with efficient capacity to promote plant growth and yield. Most studies on the Azospirillum–plant association have been conducted on cereals and annual grasses. More studies are needed in perennial pastures, such as bermudagrass (Cynodon dactylon (L.) Pers.) that require substantial nitrogen (N) fertilization to maximize their production potential. Therefore, pastures based on Tifton 85 bermudagrass in association with annual ryegrass (Lolium multiflorum Lam.), which were inoculated with A. brasilense and fertilized with increasing amounts of N fertilizer and grazed by lactating Holstein cows were evaluated. Three grazing systems were evaluated: (i) Tifton 85, inoculated + 180 kg N/ha per year; (ii) Tifton 85 + 230 kg N/ha per year; and (iii) Tifton 85 + 280 kg N/ha per year. Forage samples were collected before and after grazing to evaluate the responses of the plants and animals. The forage yields of the systems were 21.0, 20.8 and 22.1 t DM/ha per year and the stocking rates were 3.9, 3.8 and 4.0 animal unit/ha per day, respectively. Crude protein, total digestible nutrients and neutral detergent fibre concentrations were 162, 560 and 667 g/kg, respectively. Inoculation in pastures planted with Tifton 85 bermudagrass in combination with ryegrass (plus 180 kg N/ha per year) had a positive effect, providing forage yield and nutritional value equivalent to those with fertilization with 230 kg N/ha per year.

Calcium content in the diet can affect egg quality and production in aged birds. Calcium pidolate is a new form of calcium supplementation in poultry diets, which favours the absorption of this mineral. The current study was conducted to evaluate the effect of dietary calcium pidolate concentration in the diets of aged laying Japanese quails. A total of 120 female quails (27 weeks old) were assigned to four treatment groups, each with six replicates of five quails per pen. In the 10-week trial, quail diets were supplemented with calcium pidolate at four concentrations (0, 0.25, 0.50 and 1.00 g/kg), providing 25.1, 25.4, 25.8 and 26.5 g/kg calcium, respectively. As a result of this study, body weight, feed intake and feed conversion ratio did not change with the concentration of calcium pidolate (P > 0.05). In contrast, albumen and yolk index decreased as calcium pidolate level increased (P < 0.05). Moreover, calcium pidolate at a concentration of 0.50 g/kg resulted in the greatest egg production, eggshell breaking strength and eggshell thickness (P < 0.05), while above these levels the factors studied were negatively affected. According to these results, the optimal concentration of calcium pidolate in the diets of aged laying quails was 0.50 g/kg.

To overcome grass supply shortages on the main grazing block, some pasture-based dairy farmers are using zero-grazing (also known as ‘cut and carry’), whereby cows are periodically housed and fed fresh grass harvested from external land blocks. To determine the effect of zero-grazing on cow performance, two early-lactation experiments were conducted with autumn and spring-calving dairy cows. Cows were assigned to one of two treatments in a randomized complete block design. The two treatments were zero-grazing (ZG) and grazing (G). The ZG group were housed and fed zero-grazed grass, while the G group grazed outdoors at pasture. Both treatments were fed perennial ryegrass (Lolium perenne L.) from the same paddock. In experiment 1, 24 Holstein Friesian cows (n = 12) were studied over a 35-day experimental period in autumn and offered fresh grass, grass silage, ground maize and concentrates. In experiment 2, 30 Holstein Friesian cows (n = 15) were studied over a 42-day experimental period and offered fresh grass and concentrates. Average dry matter intake and milk yield was similar for ZG and G in both experiments. Likewise, ZG did not have an effect on milk composition, body condition or locomotion. Zero-grazing had no effect on total nitrogen excretion or nitrogen utilization efficiency in either experiment, or on rumen pH and ammonia concentration in experiment 1. While zero-grazing may enable farmers to supply fresh grass to early-lactation cows in spring and autumn, results from this study suggest that there are no additional benefits to cow performance in comparison to well-managed grazed grass.

A data set of individual observations was compiled from 17 digestibility trials with sheep with the aim of evaluating the impact of the method of analysis and, within each method, the effect of dietary fibre content and trial on the faecal recovery rate (RR) of indigestible dry matter (iDM) and indigestible neutral detergent fibre (iNDF). Markers concentration in samples of diet and faeces were determined either in polyester filter bags of 40 μm of porosity incubated in situ for 144 h (method A; n = 257) or in bags of 16 μm of porosity incubated in situ for 288 h (method B; n = 321). Regardless of the incubation method, the intake of either iDM or iNDF was linearly (P < 0.05) related to the amount excreted in faeces and, for all linear relationships, the slopes were lower than 1 (i.e. between 0.53 and 0.71). Within both methods, the RR of both markers linearly decreased at increased NDF content in diet (P < 0.05). The root mean square error of the linear relationships varied from 0.147 to 0.189 which represented from 19% to 24% of the respective average RR of markers. In conclusion, regardless of the incubation method, the RR of both markers showed high individual variability and, thus, the use of average RR values obtained in controlled digestibility trials as an index to correct the individual digestibility values obtained with either marker in field trials has the potential to improve the accuracy but not the precision of the digestibility estimates.

One of the most important aspects of genetic evaluation (GE) is the definition of contemporary groups (CG), commonly defined as animals of the same sex born in the same herd, year and season. The objective of this study was to use an aridity index (AI) to classify season and evaluate the implications on the GE of Braunvieh cattle. A data set with 32 777 and 22 448 birth weight (BW) and weaning weight adjusted to 240 days (WW) records, respectively, was used to compare two methods of classification of climatic seasons to be used in the definition of CG for GE models. The first method considered rain season criterion (RC), and the second method is a proposed classification using an AI. Both methods were compared using two approaches. The first approach examined differences in mixed models using the RC and AI season to select the best model for BW and WW, evaluated by different goodness of fit measures. The second approach considered fitting a GE model including the season classifications into the CG structure. Lower probability values for season effect and better goodness of fit measures were obtained when the season was classified according to the AI. Results showed that although differences are small, the AI allows a better model fitting for live-weight traits than RC and revealed a re-ranking effect on expected progeny differences data. Further analysis with other traits would demonstrate the extended utility of AI indicators to be considered for fitting models under a climatic change environment.

This experiment was conducted to evaluate the impact of substituting rice straw with citrus pulp (CRP), sweet potato peels (SPP) and vines (SPV) on fermentation quality and in vitro digestibility when ensiled with or without wet brewers' grains (WBG). Seven treatments were set as follows: (i) rice straw only (control); (ii) 800 g rice straw/kg + 200 g CRP/kg (RC); (iii) 720 g rice straw/kg + 180 g CRP/kg + 100 g WBG/kg (RC+); (iv) 800 g rice straw/kg + 200 g SPP/kg (RP); (v) 720 g rice straw + 180 g SPP/kg + 100 g WBG/kg (RP+); (vi) 800 g rice straw/kg + 200 g SPV/kg (RV); (vii) 720 g rice straw/kg + 180 g SPV/kg + 100 g WBG/kg (RV+). After ensiling for 3, 7, 14, 30 and 60 days, silos were opened for fermentation profile, aerobic stability and in vitro parameter analyses. All substitution types improved fermentation quality, characterized by significantly lower pH and NH3-N content, higher lactic acid content and V-scores. WBG application promoted hetero-fermentation and significantly increased DM losses, acetic acid and ethanol contents. After 60 days of ensiling, the NDF, ADF and cellulose contents in mixed silages significantly decreased compared to control. During aerobic exposure, WBG application significantly prolonged aerobic stability. SPV substitution significantly improved in vitro digestibility of dry matter, NDF and ADF. In conclusion, the SPP substitution combined with WBG application was recommended as a result of the optimal balance of fermentation quality, aerobic stability and in vitro digestibility.

The water balance and urinary parameters of lambs fed diets containing cactus cladodes varieties were evaluated. Thirty-six uncastrated male Santa Inês lambs (22.0 ± 2.9 kg initial body weight) were distributed in a completely randomized design, with three treatments and 12 replicates. The animals were fed a control diet (Tifton-85 hay as exclusive roughage), Miúda or Orelha de Elefante Mexicana (OEM) cactus-based diets. Urine samples were collected 45 days after the introduction of the tested diets, and voluntary water intake was measured over 10 days, during the experimental period. Diets containing Miúda or OEM cactus caused a reduction of 68.35 and 77.03% in voluntary water intake, respectively. In addition, the Miúda cactus cladodes caused urinary alkalinization (pH = 8.14) and reduction in the urinary excretion index (UEI) and on the fractional excretion rate (FER) of total calcium (Ca). The diets with OEM cactus cladodes caused higher oxalates intake (6.21 g/day) and increase in urinary volume. Regardless of the variety, there was a reduction in the urinary concentrations of urea, creatinine, total proteins, Ca, phosphorus (P), UEI of urea and P, as well as in FER of P, compared to the control diet. Urinary Mg content and UEI of uric acid and Mg increased and FER of P decreased by diets supplemented with different varieties of cactus cladodes. In conclusion, the cactus cladodes increased water intake and retention, and diuresis in lambs, altered urine parameters, without compromising kidney function.

The great demographic pressure brings tremendous volume of beef demand. The key to solve this problem is the growth and development of Chinese cattle. In order to find molecular markers conducive to the growth and development of Chinese cattle, sequencing was used to determine the position of copy number variations (CNVs), bioinformatics analysis was used to predict the function of ZNF146 gene, real-time fluorescent quantitative polymerase chain reaction (qPCR) was used for CNV genotyping and one-way analysis of variance was used for association analysis. The results showed that there exists CNV in Chr 18: 47225201-47229600 (5.0.1 version) of ZNF146 gene through the early sequencing results in the laboratory and predicted ZNF146 gene was expressed in liver, skeletal muscle and breast cells, and was amplified or overexpressed in pancreatic cancer, which promoted the development of tumour through bioinformatics. Therefore, it is predicted that ZNF146 gene affects the proliferation of muscle cells, and then affects the growth and development of cattle. Furthermore, CNV genotyping of ZNF146 gene was three types (deletion type, normal type and duplication type) by Real-time fluorescent quantitative PCR (qPCR). The association analysis results showed that ZNF146-CNV was significantly correlated with rump length of Qinchuan cattle, hucklebone width of Jiaxian red cattle and heart girth of Yunling cattle. From the above results, ZNF146-CNV had a significant effect on growth traits, which provided an important candidate molecular marker for growth and development of Chinese cattle.

Wet winter conditions can create animal welfare issues in feedlots if the pen surface becomes a deep, wet, penetrable substrate. Feedlot pens with a clay and gravel base (N = 30) bedded with 150 mm (W15) and 300 mm (W30) depth of woodchips were compared to a control treatment with no bedding over a 109-day feeding period, while irrigated to supplement natural rainfall. The pad substrate was measured for variables which would affect cattle comfort and value of the substrate for composting. The penetrable depth of control pens was higher than both woodchip-bedded treatments from week 2, and increased until the end of the experiment. Meanwhile these scores were steady for W30 throughout the experiment, and increased for W15 only after week 10. Moisture content of the pad was higher throughout the experiment in the control pens than in the woodchip-bedded pens. In the control pens, the force required to pull a cattle leg analogue out of the pen substrate was three times that required in woodchip-bedded treatments. The W15 treatment increased C : N in the substrate to the upper limit of suitability for composting, and in W30, C : N was too high for composting after a 109-day feeding period. Overall, providing feedlot cattle with 150 or 300 mm of woodchip bedding during a 109-day feeding period improved the condition of the pad substrate for cattle comfort by reducing penetrable depth and moisture content of the substrate surface stratum, but composting value decreased in W30 over this feeding period duration.