Hierarchical (each dam mated to one sire) and factorial (each dam mated to several sires) designs involving juvenile selection in a closed nucleus breeding programme were compared for rates of genetic response and inbreeding using stochastic simulation. Numbers of sires and dams selected and herd sizes varied. Generations were discrete. Sires and dams were selected at 15 months on the basis of an estimated breeding value (EBV) calculated using selection index which considered information on close relatives. Selection was for total merit index. Matings were carried out assuming use of multiple emulation and embryo transfer (MOET) or in vitro embryo production (1VEP) technologies, over a range of reproductive parameters. Reproductive rates assumed a fixed number of potential offspring per collection with IVEP and MOET. Comparison of mating designs using MOET showed that rates of genetic response and inbreeding with factorial designs voere from 88 to 431% and 36 to 111% of those with the hierarchical design. Generally rates of genetic response were increased and inbreeding decreased by increasing the number of mates per dam due to a reduction of correlations among EBV. Reduced rates of genetic response were occasionally observed in factorial designs involving the mating of dams to large numbers of sires. This increased the generation interval due to the time to carry out the matings. Factorial designs were found to be most efficient for increasing genetic response and or decreasing inbreeding, for breeding programmes involving the selection of equal numbers of sires and dam. For the breeding programmes considered, rates of genetic response with IVEP equalled the best of the MOET designs, achieved at highest MOET rates, at the lowest level of IVEP. But at the equivalent rate of genetic response, rate of inbreeding was increased by 8%. The increased rate of inbreeding was due to the shorter generation interval with IVEP, and the increased probability of selecting related individuals assuming all donors respond to IVEP.

This study examined whether a prolonged anoestrus had any long-term effect on subsequent fertility of cows and estimated the relationship between repletion and resumption of reproductive activity. Twelve low body-condition, non-milking, non-cycling (depletion state) F1 crossbred dairy cows (Friesian × Boron and Simmental × Boran) were stratified to two diets (H: natural grass hay offered ad libitum and mineral lick +3 kg concentrate, and H + P: H + 7 h/day natural pasture grazing) according to parity, body weight, body condition score and calving intervals. Daily dry-matter intake was similar between cows on the two diets, but total intake of nitrogen was proportionately about 0·10 greater for cows with access to pasture. The calculated metabolizable energy intake was more than twice the estimated maintenance requirement for cows on both diets. Live weights increased from depletion to ovulation, to oestrus and to conception, but were not significantly different between cows on both diets. Body condition score increased from depletion time to first oestrus and to repletion and was greater for H + P than for H cows at first oestrus and at conception. After an average of 45 days of repletion, cows were already ovulating with no significant differences between cows on either diet. Days to onset of oestrus were 83 and 44 days for diet H and H + P, respectively. Time to conception was similar between coivs on both diets. Conception occurred when cows on H and H + P diets had recovered proportionately 0·51 and 0·58 of their live-weight and 0·84 and 1·27 of their body condition loss, respectively. Interval to repletion weight was 178 and 139 days for cows on the H and H + P diet, respectively. Cows subjected to an exceptionally long depletion period were able to resume ovarian cyclic activity and to conceive in less than 3 months when given twice maintenance requirements. These results have important management implication for on-farm situations in the tropics where fluctuations of food availability and quality occur.

Over a 15-year period 1011 calving intervals from 187 Brahman (B) cows and 687 calving intervals from 146 Santa Gertrudis (SG) were examined. Year of calving significantly affected intervals in both breeds. Season of calving and parity of dam were both important in SG. Longer intervals were seen in B than SG (437 v. 406 days). Repeatability of calving interval was low (< 0·02). The average life-time calving interval was significantly related to total calf production in that cows with shorter mean intervals had more calves in their lifetime.

Records on total of 1746 calvings of principally Milking Criollos and their crosses were collected between 1972 and 1990 at the experiment station of the Mexican Association of Animal Production on the Gulf Coast of Mexico. Traits of interest were 305-day milk yield, days in lactation, number of services per lactation (raised to power 1/2), age at first calving and lifetime milk yield. Data were analysed by restricted maximum likelihood (REML) under an individual animal model based on 584 animals of which 146 were female ancestors and 35 were male ancestors. Heritabilities of 305-day milk and age at first calving were 0·17 and 0·07, respectively, but heritabilities of other traits were close to zero. Genetic trend in the Milking Criollos for 305-day milk was small and not significant (0·76 (s.e. 2·38) kg/year). Of crosses with Criollo sires, those involving Holstein and Canadienne breeding had highest 305-day and lifetime milk yields and those involving Brown Swiss and native Mexican (mostly Oaxaca) had lowest yields. Jersey crosses were intermediate for 305-day yield. The F1 Criollo × Jersey cross had highest lifetime yield, but backcrosses involving Jersey breeding were poor for lifetime milk. Additive breed effects for Jersey and Canadienne, relative to Milking Criollo, were 88 (s.e. 91) and 227 (s.e. 74) kg 305-day milk, respectively. Heterosis was 144 (s.e. 55) kg (11·6%) for 305-day milk, 16–4 (s.e. 9·6) (5·0%) for days in milk, −0·107 (s.e. 0–042) (7·7%) for number of services per lactation raised to power 1/2, −25·6 (s.e. 41·4) days (2•3%) for age at first calving and 1789 (s.e. 664) kg (60·0%) for lifetime milk. A plan was designed to develop a nucleus breeding scheme utilizing multiple ovulation and embryo transfer technology (MOET) for the genetic improvement of the Milking Criollo breed in Mexico.

Selection indices to improve lean meat production of terminal sire breeds of sheep were derived. An aggregate breeding value which comprised growth rate, killing-out proportion and carcass lean proportion was examined. Selection criteria included growth rate and ultrasonic or X-ray computer tomography (CT) estimates of carcass composition. Correlations betwen the index and the aggregate breeding value were about 0·38 for indices using ultrasonic fat measurements, and about 0·40 for those using CT measurements. Dropping ultrasonic or CT measurements from the indices reduced the correlations with the aggregate breeding value by only about 0·01 and 002 respectively, indicating that selection on growth rate alone under current economic conditions was highly efficient. Changes in economic weights, and genetic parameters had little effect on the efficiency of selection. The total national discounted value of returns from a single round of selection on the indices was expected to be between £6 and £30·5 million, depending on penetration rates of improved stock, the breeding scheme used, and with a time horizon of 20 years.

1. Bulls and steers of the Yugoslav Simmental breed (38 in total) were reared and fattened to 470 kg live weight. Half received a moderate level of feeding to 8 months of age and half a high level of feeding over this period; all were fed on a high level from 8 months to slaughter, their diet involving a concentrate mixture, lucerne hay and maize silage.

2. At slaughter weight, steers were 2·5% higher at withers than bulls, but other body measurements showed no significant difference.

3. Castrates needed a 3 to 8% longer fattening period depending on the feeding treatment.

4. Castration reduced the rate of live-weight gain by 4·9% over the whole rearing and fattening period, and by 8·0% over the fattening period only.

5. Bulls had better feed conversion ratios overall. Steers consumed more starch units per kg of live-weight gain—8·1% more over the whole rearing and fattening period and 9·8% over the fattening period. Consumption of digestible crude protein was also higher in steers.

6. The detrimental effect of castration was more strongly expressed on the HH feeding treatment. An increase in the concentration of the rations did not improve food conversion ratios.

In determining the milk yield of the sow by weighing the litter before and after suckling, loss of weight due to metabolic processes must be considered.

An experiment is described to investigate the loss of weight of piglets caused by metabolic processes between two consecutive weighings, and to investigate the relationship between this loss on the one hand and the body weight and the age of the piglets on the other. Weight changes of 15 litters of average 9·8 piglets were studied during 66 weighing periods of 16 minutes, by putting the pigs in a cage, fixed on a differential balance. Each weighing period consisted of 8 periods of 2 minutes, so that in all, loss of weight was studied during 528 2-minute periods.

The relative importance of the breed of lamb and the breed of dam in determining the copper status of the lamb until 24 weeks of age was investigated by transferring embryos within and reciprocally between Scottish Blackface and Welsh Mountain ewes. A diet low in Cu (2·4 mg/kg dry matter (DM) was fed both to the ewes from mating onward and subsequently to their lambs.

At birth, lambs with Welsh dams had greater concentrations of Cu in their livers than lambs with Blackface dams. This difference, although reduced, still remained at 9 weeks of age (weaning), when lambs with Welsh dams also had greater concentrations of Cu in plasma.

By 24 weeks of age, Welsh lambs, irrespective of their dams' breed, had greater concentrations of Cu in both liver and plasma than Blackface lambs, although the average Cu concentration in the liver was below 10 mg/kg DM in both breeds.

Results are also given for concentrations of Cu in kidney, brain and milk.

The experiment suggests the importance of maternal breed in determining the susceptibility, both of the foetus and of the young lamb to problems associated with Cu deficiency.

Four steers from a group of eight British Friesian steers were implanted with 300 mg trenbolone acetate and 30 mg hexoestrol at the beginning of a 90-day trial period. The remainder were untreated. They were offered diets that varied in estimated content of metabolizable energy as follows (MJ/day): day 0 to 34 (period A), 100; day 35 to 60 (period B), 50; and day 61 to 90 (period C), 75 increasing to 110. Implanted steers gained significantly more live weight in periods A and C, and lost less in period B, than controls. Implanted steers had significantly elevated concentrations of plasma glucose in period A, and lower values for plasma urea and serum albumin throughout. Differences between control and implanted steers for the other blood constituents studied, including growth hormone, insulin and prolactin, were small and not significant. The main effects of changes in dietary energy intake on blood composition included significant increases in both groups of animals in the concentration of free fatty acids and growth hormone during underfeeding (period B). These concentrations decreased in period C, concomitant with significant increases in the concentrations of insulin and prolactin.

The purpose of the study was to investigate the rôle of novel mitochondrial uncoupling proteins UCP2 and UCP3 in the poor efficiency of energy utilization observed in piglets offered a low protein diet. Eight male piglets (Landrace × Large White) from different litters were allocated randomly to one of two groups; a high protein group (HP) was given restricted amounts of a diet containing 270 g/kg of crude protein whereas the low protein group (LP) was offered ad libitum a diet containing 27 g/kg of crude protein. The experimental period extended over 4 weeks, including an initial period of 1 week for adaptation. Live weights were recorded weekly and food residues were weighed daily. During the 2nd and 3rd weeks, faeces were collected for measurement of digestible energy intake. Over the last 2 weeks whole body oxygen consumption was measured by indirect calorimetry before, 1·5 h after and 3·5 h after the start of feeding. The piglets were killed at the end of week 4 and tissue expressions of UCP2 and UCP3 were measured by Northern blot analyses using a partial length pig UCP2 probe that had high homology to both UCP2 and UCP3. The HP group maintained their live weight during the experimental period whereas the live weight of the LP group increased significantly (P < 0·01) but only slightly despite a digestible energy intake that was more than twice that of the HP group (P < 0·01). Whole body oxygen consumption of the LP piglets was about three times that of the HP piglets (P < 0·05), with similar increases 1·5 h after feeding (P < 0·05) in both groups indicating increased thermogenesis. A marked increase in the expression of UCP2 and UCP3 was observed in the skeletal muscle, adipose tissue and spleen of the LP piglets. The results are consistent with a rôle for UCP2 and UCP3 in diet-induced thermogenesis in piglets and specifically in the regulation of efficiency of energy utilization in pigs.

The ruminal activity, daily live-weight gain and feed conversion efficiency of zebu calves inoculated with whole rumen contents from a mature cow were compared with those of calves maintained under the same conditions except that they were ciliate-free and isolated from other ruminants.

A thriving mixed population of ciliate protozoa became established in the rumen of calves two weeks after inoculation. Entodinium was the first genus to appear and was followed by Isotricha, Polyplastron, Ophryoscolex, Diplodinium and Dasytricha.

The ruminal volatile fatty acid and ammonia concentrations in the inoculated calves showed a pronounced rise above the values found in the uninoculated controls.

The inoculated calves grew faster and had a higher feed conversion efficiency than the uninoculated controls.

The magnitude of the discrepancy between conventional regression estimates of protein retention efficiency and theoretical estimates of synthesis efficiency indicates a major contribution ascribable to protein turn-over in the generally accepted estimates. As protein turn-over is known to be influenced by diet, feeding level and degree of maturity, this suggests the development of an estimator of protein efficiency that can be adapted for such differences. Therefore, based on generally accepted formulas for growth description, a method of estimating protein retention efficiency was developed which is flexible enough to accommodate different diets, feeding levels and degrees of maturity. Moreover, a formula was derived to convert one type of estimate to the other by regarding constant efficiency as equivalent to variable efficiency at the mid point of the estimation interval. Increase in scientific depth to this descriptive approach is provided by a theoretical consideration of a possible mechanism of hormonal control of protein synthesis and breakdown, ultimately expressed as proportionalities to powers of whole body protein (P). Molecular considerations on cellular synthesis and breakdown indicate a difference between breakdown and synthesis powers equal to (2/9)Q. The factor (2/9) is indicated by an argument based on insulinlike growth factor derived activator diffusion attributes by nucleus and body tissue geometries, while Q is equal to the proportion of nuclei activated by insulin-like growth factor. This proportion is likely to be a function of the concentration of growth factor in the blood. Hence, a linear relationship between intake and blood insulin-like growth factor concentration suggests that Q can be represented by a scaled transformation of intake, 0 ≤ Q ≤ 1, such that a value of Q = 1 represents ad libitum intake on a suitable diet and Q = 0 intake at the maintenance requirement. The quantification of breakdown and synthesis power differences by (2/9)Q leads to kP = {1 + [1 − (P/α)(2/9)Q]−1/6}−1, for turn-over related protein retention efficiency (kP), with α the limit value of P at maturity, so that 0 ≤ (P/α) ≤ 1. Experimental estimates, derived from direct estimates of whole body protein synthesis and breakdown at predetermined levels of intake, are in excellent agreement with the theoretical (2/9)Q in the power associated with (P/α) in kP. Furthermore, conventional multiple regression retention efficiencies satisfactorily approximate the turn-over related retention efficiency that can be calculated at a given level of intake for the mid point of the interval covered by the regression estimates.

1. A growth trial was carried out using 24 British Friesian steers given diets containing (1) 100%, (2) 60%, (3) 40% and (4) 0% concentrates and chopped dried grass up to 100%.

2. Daily dry-matter intake was significantly lower for steers on Treatment 1 than for steers on the other three treatments.

3. Rate of live-weight gain declined from 1·18 to 0·88 kg/day and carcass gain from 625 to 430 g/day between steers on Treatments 1 and 4.

4. Killing-out percentage dropped from 55·3% (1) to 51·9% (4) and the 8th-10th rib from steers on Treatments 3 and 4 contained significantly more crude protein and less ether-extractable matter than ribs from steers on Treatments 1 and 2.

1. Three measurements on both sides of 15 carcasses and 5 additional measurements on bot h sides of 6 carcasses were taken in duplicate by 3 observers working independently. The carcasses, which ranged in weight from 520 to 884 lb., were measured from 2 to 6 hr. after slaughter while on the hook in the chill-room of a slaughter-house.

2. Length of body and loin were the measurements which could be taken the most consistently, whereas the 2 depth measurements and the length of tibia were the least repeatable.

3. For 2 measurements, there were marked differences between observers i n the average differences between sides. In all carcass investigations, measurements should be taken on both sides and averaged.

4. For all measurements, except the length of body and loin, there were differences in the average values found by the 3 observers.

5. It is suggested that to achieve a satisfactory degree of repeatability, slight redefinition and clarification of the points of reference is required for some measurements, whereas others may have to be discarded.

Hybrid vigour effects were measured on weights at birth and at 3, 6, 9 and 12 months, in crosses between Red Poll and Boran cattle. Means of crossbred weights were superior to means of purebred weights by 9·7% at birth and by 26·4% to 29·7% at 3–12 months. Boran cows had superior mothering ability to Red Poll cows, judged by the early growth of reciprocal cross calves, and lost less body weight over a nine-month lactation period.

Gut cell losses contribute to overall feed efficiency due to the energy requirement for cell replenishment. Intestinal epithelial cells are sloughed into the intestinal lumen as digesta passes through the gastrointestinal tract, where cells are degraded by endonucleases. This leads to fragmented DNA being present in faeces, which may be an indicator of gut cell loss. Therefore, measuring host faecal DNA content could have potential as a non-invasive marker of gut cell loss and result in a novel technique for the assessment of how different feed ingredients impact upon gut health. Faecal calprotectin (CALP) is a marker of intestinal inflammation. This was a pilot study designed to test a methodology for extracting and quantifying DNA from pig faeces, and to assess whether any differences in host faecal DNA and CALP could be detected. An additional aim was to determine whether any differences in the above measures were related to the pig performance response to dietary yeast-enriched protein concentrate (YPC). Newly weaned (∼26.5 days of age) Large White × Landrace × Pietrain piglets (8.37 kg ±1.10, n = 180) were assigned to one of four treatment groups (nine replicates of five pigs), differing in dietary YPC content: 0% (control), 2.5%, 5% and 7.5% (w/w). Pooled faecal samples were collected on days 14 and 28 of the 36-day trial. Deoxyribonucleic acid was extracted and quantitative PCR was used to assess DNA composition. Pig genomic DNA was detected using primers specific for the pig cytochrome b (CYTB) gene, and bacterial DNA was detected using universal 16S primers. A pig CALP ELISA was used to assess gut inflammation. Dietary YPC significantly reduced feed conversion ratio (FCR) from weaning to day 14 (P<0.001), but not from day 14 to day 28 (P = 0.220). Pig faecal CYTB DNA content was significantly (P = 0.008) reduced in YPC-treated pigs, with no effect of time, whereas total faecal bacterial DNA content was unaffected by diet or time (P>0.05). Faecal CALP levels were significantly higher at day 14 compared with day 28, but there was no effect of YPC inclusion and no relationship with FCR. In conclusion, YPC reduced faecal CYTB DNA content and this correlated positively with FCR, but was unrelated to gut inflammation, suggesting that it could be a non-invasive marker of gut cell loss. However, further validation experiments by an independent method are required to verify the origin of pig faecal CYTB DNA as being from sloughed intestinal epithelial cells.

This study was conducted by two trials to investigate effects of sequential feeding with low- and high-protein diets on growth traits and plasma metabolites in geese. In Trial I, the effect of sequential feeding under time-restricted feeding system was investigated. Seventy-two White Roman goslings were randomly allotted into either sequential feeding (S1) or control feeding (C1) group. All goslings were fed for 1 h at morning and at evening, respectively, from 2 to 8 weeks of age. S1 group was offered 13% CP diet at morning and 19% CP diet at evening. C1 group was offered the same diet (16% CP; mixed equally with the two diets mentioned above) at both morning and evening. Blood samples were hourly collected for 4 h after feeding at both morning and evening for the determination of the postprandial plasma levels of glucose, triacylglycerol and uric acid at the end of experiment. Results showed that BW, average daily gain (ADG), and daily feed intake (FI) were not different between groups, but the feed efficiency (FE) in S1 group was significantly higher than that in C1 group (P<0.05). The areas under curve (AUC) of plasma postprandial levels of glucose, triacylglycerol and uric acid were not affected by treatment, but the AUC of triacylglycerol and uric acid in morning were lower than those in evening (P<0.05). In Trial II, the effect of sequential feeding under ad libitum feeding system was investigated. Twenty-four goslings were randomly allotted into either sequential feeding (S2) or control feeding (C2) group. Diets were altered at 0600 and 1800 h, respectively, and geese were fed ad libitum from 4 to 8 weeks of age. S2 group was offered 14% CP diet at morning and 20% CP diet at evening. C2 group was supplied the same diet (mixed with the two diets according to the ratio of diets consumed by S2 group on the preceded day) at both morning and evening. Results showed that the ADG in S2 group was higher than those in C2 group (P<0.05). Summarized data from both trials showed that sequential feeding improves daily gain and FE in growing geese.

While beneficial for sow reproductive efficiency and biosecurity, segregated early weaning (SEW) leads to a systemic immune response that adversely affects the digestive physiology and post-weaning growth of pigs. Two experiments were conducted to evaluate the effects of a glucocorticoid receptor agonist (GA) on growth performance, measures of immune function and intestinal integrity of SEW pigs. In both experiments, pigs were fed corn-soybean meal-based starter diets. In the first experiment, 48 pigs (initial BW 4.8 ± 0.7 kg) were weaned at 21 ± 1 days and randomly assigned to three GA treatment groups: 0, 0.2 and 0.6 mg GA/kg of BW injected intramuscularly. Treatments were administered one day before weaning. Pigs in the 0 mg GA group received sterile saline in place of GA. Body weight was measured daily from one day before to 7 days post-weaning, and then weekly until 28 days post-weaning. Piglets treated with 0.2 mg GA had a higher BW than piglets in other treatment groups during the 28-day course of the study (P <0.02). To further explore the mechanisms behind this result, a second experiment was performed in which a total of 18 gilts (BW 5.6 ± 0.85 kg) were randomly assigned into three treatment groups: suckling plus saline (UWS), weaned treated with GA (WGA; 0.2 mg GA/kg BW) and weaned plus saline (CON). Treatments were administered one day before and 3 days post-weaning. The WGA and CON groups were weaned at 23 ± 2 days, while the UWS group remained with sow for the duration of the study. Body weight was measured daily and blood plasma was collected at 0, 1, 4 and 5 days post-weaning. All gilts were euthanized 5 days after weaning and jejunum samples were collected for mucosal scrapings, histomorphological analysis and gene expression analysis. Plasma levels of interleukin-1β (IL-1β) and haptoglobin were lower in WGA pigs compared with CON (P <0.02), while plasma total antioxidant capacity was higher in WGA pigs compared with both CON and UWS groups (P <0.01). Relative to CON, GA downregulated IL-18 gene expression in the jejunum, as assessed by both tissue homogenate and mucosal scrapings, but it upregulated claudin-IV gene expression only in the tissue homogenate (P <0.01). These results suggest that GA treatment improves the growth performance of SEW pigs in part by mitigating the negative effects of systemic inflammation. However, the effect of GA on barrier integrity requires further investigation.

Mitochondrial (mt) DNA D-loop heterogeneity, haplotype distribution and possible sub-population structures within the relevant populations are important for DNA-based traceability. To gain insight into this distribution, we compared 1515 Bos taurus mtDNA D-loop sequences available from GenBank to 219 sequences that we sequenced de novo. A pronounced ambiguous trace typical of C-track length heteroplasmy was encountered in 5% of the samples, which were excluded from the analysis. Previously undescribed mutations and haplotypes were observed in 6% and 63% of the sequences, respectively. B. taurus haplotypes divided into the taurus, indicus and grunniens types and 302 variable sites formed the 858 taurus haplotypes detected. Fifty-five sites displayed a complex level of variation. As each level represents an independent mutation event, a total of 399 mutations were traced, which could potentially explain independent formation of less than half (47%) of the haplotypes encountered: most haplotypes were derived from different combinations of these mutations. We suggest that a mutational hotspot may explain these results and discuss the usefulness of mtDNA for identity and maternity assurance.

Reducing the CP level of the diet allows for a reduction in N excretion without limiting performance as long as the amino acid (AA) requirements are covered. The availability of crystalline AA has permitted for a considerable reduction in the CP level of diets, practically used in pig nutrition. The adoption of low CP diets and the extent to which the CP content can be reduced further depends on the knowledge of the minimum levels of indispensable AA that maximize growth. The standardized ileal digestible (SID) Phe : Lys and Tyr : Lys requirements and the possibility to substitute Tyr by Phe have never been studied in piglets. The objectives of this study were to estimate these requirements in 10 to 20 kg pig as well as to determine the extent to which Phe can be used to cover the Tyr requirement. In three dose–response studies, six pigs within each of 14 blocks were assigned to six low CP diets (14.5% CP) sub-limiting in Lys at 1.00% SID. In experiment 1, the SID Phe : Lys requirement estimate was assessed by supplementing a Phe-deficient diet with different levels of l-Phe to attain 33%, 39%, 46%, 52%, 58%, and 65% SID Phe : Lys. Because Phe can be used for Tyr synthesis, the diets provided a sufficient Tyr supply. A similar approach was used in experiment 2 with six levels of l-Tyr supplementation to attain 21%, 27%, 33%, 39%, 45% and 52% SID Tyr : Lys. Phenylalanine was supplied at a level sufficient to sustain maximum growth (estimated in experiment 1). The SID Phe : Lys and SID Tyr : Lys requirements for maximizing daily gain were 54% and 40% using a curvilinear-plateau model, respectively. A 10% deficiency in Phe and Tyr reduced daily gain by 3.0% and 0.7%, respectively. In experiment 3, the effect of the equimolar substitution of dietary SID Tyr by Phe to obtain 50%, 57%, and 64% SID Phe : (Phe+Tyr) was studied at two limiting levels of Phe+Tyr. From 57% to 64% SID Phe : (Phe+Tyr), performance was slightly reduced. In conclusion, it is recommended not to use a Phe+Tyr requirement in the ideal AA profile but rather use a SID Phe : Lys of 54% and a SID Tyr : Lys of 40% to support maximal growth.