One of the early changes upon tuber induction is the switch from apoplastic to symplastic unloading. Whether and how this change in unloading mode contributes to sink strength has remained unclear. In addition, developing tubers also change from energy to storage-based sucrose metabolism. Here, we investigated the coordination between changes in unloading mode and sucrose metabolism and their relative role in tuber sink strength by looking into callose and sucrose metabolism gene expression combined with a model of apoplastic and symplastic unloading. Gene expression analysis suggests that callose deposition in tubers is decreased by lower callose synthase expression. Furthermore, changes in callose and sucrose metabolism are strongly correlated, indicating a well-coordinated developmental switch. Modelling indicates that symplastic unloading is not the most efficient unloading mode per se. Instead, it is the concurrent metabolic switch that provides the physiological conditions necessary to potentiate symplastic transport and thereby enhance tuber sink strength .

Ramp load/unload (L/UL) mechanisms are widely used to rest sliders in hard disk drives (HDDs). Loading/unloading a slider swiftly and smoothly is crucial in a HDD design. A novel, efficient simulation scheme is proposed to investigate the behaviors of a head disk interface (HDI) in ramp unloading processes. A dual scale model is enabled by decoupling the nano-meter scale change of an air bearing and the micro- or milli-meter scale deformation of a suspension. A modified Reynolds equation governing the air bearing was solved numerically. The slider design was characterized with performance functions. Three stages in an unloading process were analyzed with a lumped parameter suspension model. Key parameters for the model were estimated with a comprehensive finite element suspension model. Finally, simulation results are presented for a commercial HDI design.