A diverse assemblage of moderately well-preserved radiolarians occurs in the Ordovician cherts of the Crawford Group in the Southern Uplands terrane of the Scottish borders. A total of 8 genera and 11 species are described. Taxa present correlate to the Middle Ordovician Pygodus anserinus biozone. Key species identified include: Proventocitum procerulum, Inanigutta gansuensis, Inanigutta sp. cf. I. complanata, Inanibigutta sp. cf. I. verrucula, Inanibigutta sp. cf. I. pinglianensis, ?Inanibigutta inconstans, ?Inanihella penrosei, Haplentactinia armillata, ?Oriundogutta ramificans and Syntagentactinia sp. A new species Haplotaeniatum albaensis is introduced here and described on the basis of details of skeletal architecture observed using microcomputed tomography, demonstrating the potential of this technique as a tool for improving the understanding of radiolarian taxonomy.