The maternal metabolic environment can be detrimental to the health of the offspring. In a previous work, we showed that maternal high-fat (HH) feeding in rabbit induced sex-dependent metabolic adaptation in the fetus and led to metabolic syndrome in adult offspring. As early development representing a critical window of susceptibility, in the present work we aimed to explore the effects of the HH diet on the oocyte, preimplantation embryo and its microenvironment. In oocytes from females on HH diet, transcriptomic analysis revealed a weak modification in the content of transcripts mainly involved in meiosis and translational control. The effect of maternal HH diet on the embryonic microenvironment was investigated by identifying the metabolite composition of uterine and embryonic fluids collected in vivo by biomicroscopy. Metabolomic analysis revealed differences in the HH uterine fluid surrounding the embryo, with increased pyruvate concentration. Within the blastocoelic fluid, metabolomic profiles showed decreased glucose and alanine concentrations. In addition, the blastocyst transcriptome showed under-expression of genes and pathways involved in lipid, glucose and amino acid transport and metabolism, most pronounced in female embryos. This work demonstrates that the maternal HH diet disrupts the in vivo composition of the embryonic microenvironment, where the presence of nutrients is increased. In contrast to this nutrient-rich environment, the embryo presents a decrease in nutrient sensing and metabolism suggesting a potential protective process. In addition, this work identifies a very early sex-specific response to the maternal HH diet, from the blastocyst stage.

Hypoxia and nutrient deprivation are responsible for inducing malignant behavior in neoplastic cells. In these conditions, metabolic stress leads the cells to enhance their autophagic flux and to activate key molecules for homeostasis maintenance. Galectin-3 (Gal-3) is upregulated in pancreatic cancer and it is activated under the hypoxic atmosphere. We aimed to analyze the most effective autophagic-inducing conditions in pancreatic ductal adenocarcinoma cells and the effect exerted under these conditions in association with hypoxia on the Gal-3 expression. Gal-3 and the microtubule-associated protein light chain 3 beta (LC3) were accessed through western blot and immunofluorescence. Degradative vacuole quantification was analyzed by transmission electronic microscopy, and inhibition of Gal-3 was performed using siRNA. According to the analyses, the most effective conditions in the inducement of autophagy for PANC-1 and MIA PaCa-2 cells were nutritional deprivation and complete amino acid/glucose deprivation, respectively. PANC-1 cells presented higher Gal-3 when they were submitted to 24 h of nutritional deprivation alone and simultaneously nutritional and oxygen deprivation. Inhibition of Gal-3 causes a decrease of LC3 levels in all experimental conditions. These results confirm that Gal-3 is modulated by microenvironment factors and the possibility of Gal-3 participating in an adaptive response from PDAC cells to extreme conditions.

Mean depth and mean size of burrow system components, burrow-soil temperatures and actual diurnal temperatures, humidity and oxygen concentration in burrows of silvery mole-rats Heliophobius argenteocinereus were measured in two mesic areas in Malawi. Mesic tropic climate, together with soil and plant cover contribute to a diurnally and seasonally stable microclimate even in shallow burrows. Daily and seasonal changes of burrow temperatures were lower compared to those in bathyergids from temperate areas where ambient and burrow temperatures undergo larger fluctuations. Silvery mole-rats in Malawi are thus probably not distressed by these environmental factors.

Field studies to investigate the effect of intercropping on the relative resistance and susceptibility of cowpea cultivars to Maruca testulalis impure stands and intercropped with maize, were conducted at the International Centre of Insect Physiology and Ecology (ICIPE). It was clearly indicated that intercropping affected the relative resistance and susceptibility of cowpea cultivars. The resistance of TVU 946 to Maruca was reduced when the cultivar was under maize. This was attributed to the phenological changes observed, i.e. when planted with maize there was a significant (P = 0.05) increase in length of the pods and peduncles and significant (P = 0.05) reduction in the number of branches. Weekly mean temperatures and relative humidities indicated that there were significant differences between cropping patterns. Similarly there was a significant reduction in the photosynthetic active radiation incident on cowpea canopy in all the intercropped plots. This suggested that both intercropping and microenvironmental differences observed, reduced resistance in cultivar TVU 946 by probably acting directly on the crop, thus rendering it more susceptible.

A study of the effect of microenvironment on the pea leaf weevil's behaviour was made comparing a broad bean monoculture with a broad bean-oat crop mixture. It is observed that more weevils depart from the crop mixtures than from the monocultures. Microenvironment study in the two cropping systems show that temperature and wind speed are not significantly altered in the crop mixtures as compared to the monoculture, but beans growing in the mixtures were significantly more shaded than those in the monoculture. Evidence is given to show that this relative shading could be the main factor responsible for the observed increased weevil departure from the crop mixtures.