This research investigated the temperature and light requirements for seed germination and emergence patterns of pinnate poppy, violet horned-poppy, and nodding hypecoum, three annual Papaveraceae species found in arable lands in the Mediterranean region. Two experiments performed in growth chambers (1) analyzed light (complete darkness or 12 h light) and temperature (10/5, 15/5, and 20/10 C day/night temperatures) requirements for germination, and (2) determined base temperature (Tb) for germination. An outdoor pot trial was also set up to study emergence patterns. All species showed higher germination in complete darkness than they did with a light regime, irrespective of dormancy level, time of the year, and temperature regime under which germination was tested, illustrating better germination when seeds are buried. Tb ranged from −2.6 to 0 C, depending on the species, indicating low temperature requirements for germination. Given their higher germination in daily fluctuating, rather than constant temperatures, the three Papaveraceae species should have the capacity to form persistent seed banks. These species behaved as winter annuals (from November to February) in the pot experiment and had difficulties to emerge in spring. Given that they cannot avoid autumn–winter chemical treatments, this could partially explain their regression in arable fields. These results bring new information to develop management strategies for these Papaveraceae species in agroecosystems.

In order to improve growth chamber protocols for lichens, we tested the effect of 1) wet filter paper versus self-drained nets as a substratum for lichens, and 2) gradual versus abrupt transitions between dark and light periods. For Lobaria pulmonaria (L.) Hoffm. cultivated on nets, RGR increased by 60% compared to those on wet papers, whereas abrupt on/off transitions between day/night gave as high growth rates as gradual transitions mimicking sunrise/sunset. Because thalli on nets had less surface water than those on papers, the higher RGR on nets likely resulted from less suprasaturation depression of photosynthesis. By supporting very high growth and eliminating any visible damage, the revised growth chamber protocols facilitate new functional lichen studies.

Light and fluctuating temperatures are two important factors triggering seed germination. The aim of this work was to: (1) elucidate the effect of temperature, light regime and storage time on seed germination in nine taxa of Verbascum spp., collected from different habitats in the Mediterranean area; and (2) estimate threshold temperatures for seed germination. For all taxa, germination assays were performed at constant and fluctuating temperatures, both in continuous darkness (D) and in alternating light/dark (L/D; 12 h photoperiod). Final germinated proportions (FGPs), base (T b), optimal (T o) and cut-off (T c) temperatures were derived. At constant temperatures, seed germination was strongly suppressed under the D regime. In L/D, the effect of storage time was very small and the highest FGPs were observed from 15 to 30°C (40–100%), depending on the species. T b ranged from below 7 to above 10°C and it appeared to be constant within each seed lot. T o and T c showed some within-lot variability and were higher for fast-germinating seeds in each lot. Considering fluctuating temperatures, germination appeared to be quicker and more complete than at constant temperatures. The germination of Verbascum spp. is favoured in L/D and fluctuating temperatures, which explains their pioneer character when positioned near the soil surface and under a low vegetation canopy. V. arcturus and V. pinnatifidum were shown to be less favoured by L and fluctuating temperatures, which might explain their ability to germinate in rocky areas or sandy dunes, even when they are not directly exposed to the light.

The present work was planned to study the effects of different lighting regimes in both mild and hot periods of the year on New Zealand White doe rabbit traits. The light regimes used were natural daylight as control, 16 h light and 8 h darkness, 12 h light and 12 h darkness, and 8 h light and 16 h darkness. The traits studied were thermoregulatory parameters (respiration rate and temperatures of ear, rectum and skin), type of mating necessary, conception rate, gestation period, fur plucking, behavioural indicators of stress, litter size, litter weight, kit body weight, kit daily gain in weight, milk yield, kit milk intake, efficiency of conversion of milk into live-weight gain and kit mortality, at weekly intervals between birth and weaning at 35 days. Estimation of temperature-humidity index indicated that the doe rabbits were exposed to very severe heat stress, during the hot period of the year. Period of the year (heat stress) affected adversely many of the doe traits studied. The effects were significant (P < 0·001, 0·01 or 0·05) on thermoregulatory parameters (respiration rate and temperatures of ear, rectum and skin), litter weight at days 21 and 28 of age, kit body weight at birth, milk yield of the doe at day 7 of suckling and milk intake per kit at 7 and 14 days of age. Exposure of doe rabbits to a long daylight regime affected adversely many of the traits studied, while the contrary occurred with short daylight. The effects were significant (P < 0·001, 0·01 or 0·05) on litter size at all ages except at birth, litter weight at 14, 21 and 28 days of age, milk yield on all days studied, efficiency of conversion of milk into live-weight gain at days 7, 21 and 28 and mortality at birth and pre-weaning. There was no significant interaction between period of the year and light regime on any of the traits examined. Therefore, only the main factors are reported.

An 8-h photoperiod, compared with 12-, 16- or 23-h photoperiods, has been shown to reduce the incidence of injurious pecking and leg problems in intact male turkeys but to result in lower body weights, slower sexual development and less efficient food conversion. The effect of 8 h of illumination given as a solid photoperiod, as eight 1-h periods equally spread through the 24 h or concentrated into a 12-h part of the day on the performance of BUT 8 intact male turkeys was investigated.

Fragmentation of the daily illumination increased body weight, improved food conversion efficiency but did not significantly affect food intake. Increased sexual displaying and significantly better food conversion between 16 and 20 weeks by the two intermittently illuminated groups strongly suggested that both regimens were interpreted as stimulatory daylengths. Generally, losses due to mortality and culling were higher in both fragmented groups, while injurious pecking was higher in 8(1L: ID) birds before 5 weeks and in birds given a 12-h ‘subjective day’ after 5 weeks than in 8-h controls. Culling due to leg problems was significantly higher in birds on the 8(1L:2D) regimen but the incidence of leg imperfections was minimal (< 0·037) in all groups. Both intermittent groups but the 8(1L: 2D) in particular, showed a reduced response to hand movement across the eyes compared with 8-h controls. The controls ate proportionately more food during their 16-h dark period than did the intermittent group during their 12-h ‘subjective night’, though both groups increased their ‘nocturnal’ feeding with age.