The potassium sensitive magnesium absorption through the rumen wall may be influenced by additional dietary properties, such as diet type, forage type or forage to concentrate ratio. These properties are likely associated to rumen passage kinetics modified by dietary fibre content. The study aimed to assess the effects of rumen passage kinetics on apparent Mg absorption and retention in lactating dairy cows fed modified levels of fibre. Six lactating Red-Holstein and Holstein cows, including four fitted with ruminal cannulas were randomly assigned to a 3 × 3 cross-over design. The experimental diets consisted of early harvested low NDF (341 g NDF/kg DM) and late harvested high NDF (572 g NDF/kg DM) grass silage (80% DM) and of concentrates (20% of DM). As the low-fibre diet was excessive in protein, a third high-fibre diet was formulated to be balanced in digestible protein with the low-fibre diet to avoid any eventual confounding effects of NDF and protein excess. All diets were formulated to contain iso-Ca, -P, -Mg, -K and -Na. Passage kinetics of solid and liquid phase of rumen digesta were evaluated using ruminal marker disappearance profiles. Cows fed the low-fibre diet had compared to the other diets, an up to 40% lower solid and 26% lower liquid phase volume of rumen digesta and a 10% numerically higher fractional rumen liquid passage rate. Rumen pH lost 0.6 units and Mg concentration in the rumen liquid phase tripled when cows were fed the low-fibre diet. Faecal Mg excretion was up to 14% higher in cows fed the low-fibre diet and Mg absorbability was 12% compared to up to 19% in other diets. Urinary Mg excretion in cows fed the low-fibre diet was half of the ones in the other treatments, but Mg retention was not affected. Dietary protein excess neither affected rumen passage kinetics nor Mg absorption and retention. Absorption of Mg was correlated with rumen liquid volume which both decreased with decreasing daily NDF intake (NDFi, 11.8 ± 2.4 l/kg NDFi). Consequently, daily Mg absorption decreased by 1.32 ± 0.28 g/kg decreasing NDFi. To conclude, in addition to the known antagonistic effect of dietary K, the present data indicate that Mg absorption was dependent from NDFi which modified rumen liquid volume, but was independent of dietary protein excess likely associated to low NDF herbages.

Five experimental grass paddocks were sequentially contaminated with fresh bovine faeces containing known numbers of eggs of predominantly Haemonchus and Cooperia spp. during the 1984/85 dry season (November to March). Faecal, herbage and soil samples were examined at regular intervals between November and June in order to determine the rate of development and mortality of infective larvae (L3) in faeces, the pattern of herbage infestation with L3 and the role of faeces and soil as reservoirs of L3 during the dry season and early rains. L3 first appeared in faeces approximately 4 days post contamination (PC) and peak counts were obtained 28, 14, 7 and 14 days PC in the paddocks contaminated in December, January, February and March, respectively. The counts initially declined linearly at the rate of approximately 7535 L3/week and 10 947 L3/week in P2 and P4 respectively, due primarily to mortality but later there was an accelerated fall in the counts as the surviving L3 moved out of the faecal pads onto herbage. The overall trend of faecal larval populations in each paddock was therefore distinctly curvilinear. Although large numbers of L3 were present inside dry faecal pads in most paddocks throughout the dry season, none migrated on to herbage at that time of the year. Translation of L3 to herbage was very rapid and occurred simultaneously in all the paddocks 24 hours following the first heavy rainfall in late March. Consequently peak herbage infestations in all paddocks were coincident and occurred a few days after commencement of larval migration. The closer to the end of the dry season the contaminations were carried out, the larger were the subsequent early rains rise and the peak herbage infestation and the longer this infestation survived on herbage. No L3 were recovered from soil throughout the study, which suggests that faecal pads were the sole reservoir of L3 during the dry season and hence the source of the early rains herbage infestation.

Two experiments were undertaken to investigate the effect of herbage allowance and concentrate food level offered to twin-bearing ewes in the last 6 weeks of pregnancy on ewe and lamb performance. In each study, 96 twin-bearing Greyface (Border Leicester × Scottish Blackface), Rouge × Greyface and Texel × Greyface ewes were used. In experiment 1, the ewes were allocated to eight treatments consisting of two herbage allowances (1·3 and 2·6 kg herbage dry matter per ewe per day) and four concentrate food levels ranging from zero to 1000 g per day. In experiment 2, ewes were offered four herbage allowances (1·3, 1·75, 2·2 and 2·6 kg herbage dry matter per ewe per day) and zero or 500 g concentrates per day. In experiment 1, herbage allowance and concentrate food level had no significant effect on lamb birth weight, lamb mortality or lamb performance up to weaning. Herbage dry matter intake decreased linearly with increasing concentrate food level with a substitution rate of 18 g of herbage dry matter per 100 g concentrate food level. In experiment 2, lamb birth weight increased with increasing herbage allowance (P < 0·01) and with increasing concentrate food level (P < 0·01). However, lamb performance to weaning was unaffected by late pregnancy nutrition. The satisfactory levels of performance obtained with ewes offered grass-only diets in late pregnancy indicates that grazed grass is a high nutritive value food for twin-bearing ewes in late pregnancy.

Two trials were conducted to study the effect of the stage of maturity of fresh herbage on its digestibility by two breeds of sheep (Churra v. Merino). The sward consisted of a mixture of grasses and clover, and was harvested either in late May (early cut, EC) or in late July (late cut, LC). In each trial, 12 mature sheep of each breed were used. Each animal received a different amount of fresh herbage, so that levels of food intake ranged between 8·1 and 24·7 g dry matter (DM) per kg body weight per day. EC herbage showed higher crude protein content (116 v. 67 g/kg DM), lower neutral-detergent fibre concentration (494 v. 664 g/kg DM) and higher DM digestibility (0·687 (s.e. 0·005) v. 0·463 (s.e. 0·007)) than the LC herbage. DM digestibility coefficients observed in Churra (0·694 (s.e. 0·028) for EC and 0·476 (s.e.0·028) for LC herbage) tended to be greater than those observed in Merino sheep (0·680 (s.e. 0·021) for EC and 0·452 (s.e. 0·040) for LC herbage). With the EC herbage DM digestibility tended to decrease as the level of intake increased. Samples of dried herbage were incubated in the rumen of Churra and Merino sheep to determine the DM degradability by the in situ technique. Fractional degradation rates (c values) were significantly faster in the rumen of Churra than in Merino (0·046 (s.e. 0·003) v. 0·031 (s.e. 0·002)), with large differences between breeds in the DM disappearance rates at intermediate incubation times, and no significant differences between Churra and Merino sheep in the mean a + b values. Microbial N supply, calculated from the urinary excretion of purine derivatives, was significantly affected by the herbage maturity stage and by the level of DM intake, but not by the breed of sheep. It is suggested that differences between breeds of sheep in food digestibility may be related to their ability to digest fibrous diets.

Pats of pig faeces containing known numbers of Oesophagostomum dentatum eggs were placed on plots with bare soil, short or tall herbage on 8 occasions during 1 year. The number of eggs and larvae and the relative distribution of larvae in faeces, soil and herbage was monitored for 1 year after deposition. On 2 occasions soil from 8 selected plots was given to pigs, which were later slaughtered and examined for the presence of adult O. dentatum. Less than 1% of the deposited eggs could be recovered as infective larvae. The highest recoveries were generally made on tall herbage plots. The majority of infective larvae was found within the faecal pats, which indicates that infective O. dentatum larvae, to a large extent, do not disperse onto the herbage or into soil. The infective larval stage was reached only when the mean temperature in the weeks post-deposition was above 10 °C. This stage was reached within 1 week when the mean weekly temperature was above 13 °C. After the winter period no infective larvae could be recovered from any plots and no parasitic worms could be isolated from pigs fed soil from 8 selected plots.

In order to establish the response of dairy cow performance to concentrate supplementation in contrasting grazing conditions and for cows differing in milk yield at turn-out, three experiments were conducted. Each year, two levels of herbage allowance were studied in interaction with four (experiment 1) or three (experiments 2 and 3) levels of concentrate on two groups of 30 to 40 mid-lactation Holstein cows producing between 20 and 46 kg milk at turnout. Amount of concentrate and herbage allowance ranged from 0 to 6 kg fresh weight and from 12 to 22 kg dry matter (DM) per cow per day respectively. The supplementation led to average responses, per kg DM concentrate, of 104 kg milk, +66 g/day body-weight gain, +0·19 g/kg milk protein and -0·57 g/kg milk fat. These responses remained linear up to 4 or 6 kg according to the years and treatments. The response to the concentrate did not vary with the milk yield or composition at turn-out. The increase in the herbage allowance from 12 to 16 kg DM per cow per day (experiment 1) improved milk yield (+1·2 kg/day) and milk protein (+0·7 g/kg) while the increase from 16 to 22 kg DM (experiments 2 and 3) had less effect (+0·5 kg/day milk yield and +0·4 g/kg milk protein). There was no clear interaction between concentrate supplementation and herbage allowance. Under the usual conditions of spring pasture, with cows in mid lactation, the use of a constant level of concentrate at grazing proves to be a technique of some interest.

Annual surveys of fertilizer practice have been conducted since 1942. Since 1985, nitrogen application rates to grass have progressively declined and phosphorus application has been reduced since the mid 1970s. Increasing environmental issues and the present interest in organic farming and low input systems indicate that these trends will continue. Present overall fertilizer use for grazing on dairy farms is about 170 kg N, 10 kg P and 20 kg К per ha. Higher rates are used for intensive silage production, 200 kg N, 15 kg P and 50 kg К per ha. In contrast, recommended applications are 340 kg N, 18 kg P and 25 kg К per ha for grazing and 380 kg N, 40 kg P and 260 kg К per ha for intensive silage. Herbage yield is controlled by the amount and timing of nitrogen fertilizer applications. The major mineral content depends on herbage maturity within the growth cycle; both phosphorus and potassium contents fall with declining crude protein concentrations.

Nitrogen fertilizer generally increases both magnesium and sodium concentration whilst potassium application decreases both. The overall effect of combined applications is to minimize changes. In the critical spring and autumn periods, herbage dry-matter intakes may be as important as magnesium content and availability when assessing the risk of hypomagnesaemia in the grazing cow.

Fertilizers, including phosphorus, even in the long term, have little effect on the calcium and phosphorus contents of herbage. Nitrogen and phosphorus concentrations are significantly ocrrelated in grazed herbage, silage and hay. If environmental or economic policies were to result in a reduction in the crude protein content of grazed herbage from ca. 250 to ca. 200 g/kg dry matter, the phosphorus intake of a cow giving 30 kg milk per day would fall by 0·5 g/kg dry-matter intake. This, together with any desirable additional magnesium, should preferably be given as an oral mineral supplement rather than by attempting to modify the mineral content of herbage.

Two experiments were conducted with dairy cows to examine the effects of including white clover in perennial ryegrass swards and of offering the cows a choice between tall and short mixed perennial ryegrass/white clover swards. In the first experiment, cows grazed a perennial ryegrass sward (treatment Prg); a mixed sward of perennial ryegrass and white clover (treatment WC); treatment Prg during the night and treatment WC during the day (treatment Prg night/WC day); or they were offered a choice between Prg and WC (treatment Prg/WC). Cows in treatment WC produced more milk than cows in treatment Prg, with cows in Prg night/WC day and Prg/WC intermediate. The milk fat content of cows in treatment Prg was greater than in the other three treatments. When cows were offered both mixed grass/clover and pure grass swards (treatments Prg night/WC day and Prg/WC) they exhibited a partial preference for the WC sward and grazed it to a lower herbage height than the pure grass sward. In the second experiment, cows were offered a WC sward at 6 or 3·5 cm sward surface height, or a choice between pastures at the two heights. Cows offered only the tall sward produced most milk, and those offered only the short sward produced the least. Milk fat content was reduced for cows offered only the tall sward. Cows offered a choice of the tall or short swards spent longer grazing in total than the cows offered only tall or only short swards but there was no difference in the time spent grazing the tall and short swards.

The effects of duration of fasting on the short-term feeding behaviour of 12 grazing and 12 silage-fed lactating Holstein-Friesian dairy cows were examined. Four groups of three cows were rotated around fasting treatments of 1, 3, 6 or 13 h following a balanced Latin-square design. Herbage intakes for each treatment group were assessed over al-h period.

As intended there were no significant differences in sward characteristics between the experimental plots grazed by cows from different treatment groups. However, total dry-matter (DM) intake, biting rate and DM intake per bite, measured over the 1-h grazing period, increased significantly when the duration offasting was extended from 1 to 6 or 13 h (P < 0·05). There were only minor differences in grazing behaviour following fasting durations ofl and 3, or 6 and 13 h.

In a parallel study, undertaken to assess the influence offasting duration on appetite independently of grazing, four groups of three cows were housed indoors and offered 30 kg of a high quality grass silage. Silage DM intakes, measured over a 1-h period, increased significantly with extended fasting periods (P < 0·01), though silage DM intake was considerably lower than that of grazing cows for each fasting treatment.

These results suggest that dairy cows grazing on good quality swards may be able to compensate for increased degree of hunger by increasing both biting rate and DM intake per bite to increase DM intake rate. Although the DM intakes of silage and grazed grass followed similar patterns of increasing intake with extended fasting duration, DM intake rates were considerably higher in grazing cows for each fasting treatment.

A study urns conducted to determine the best method of preparation of silage and freshly cut forage samples for the estimation of rumen degradability by the nylon bag technique. Three types of forage were evaluated: a grass silage, a fresh grass (ryegrass) and a fresh legume (white clover). For each forage, six preparation methods were compared: chopping fresh (FRS); chopping and macerating fresh (FMC); chopping, freezing, thawing and macerating (FRZ); freeze-drying (FD); low-temperature oven-drying (45°C, 72 h) (OVD); high-temperature oven-drying (160°C, 90 min) (DHD). Dried samples were hammer-milled (2·5-mm screen). Chemical composition and rumen degradability were affected by the method of preparation. Protein fractions were sensitive to drying method, and oven-drying reduced nitrogen (N) solubility and degradability as the severity of the heating was increased. OVD and DHD gave lower estimates of N and dry matter (DM) solubility and degradability than FD. The effect of drying method on DM degradability was to reduce solubility and effective degradability, calculated for an outflow rate of 0·033 per h, but not potential degradability.

Silage DM degradability was less affected than that of fresh forages. Freshly prepared samples (FRS, FMC, FRZ) gave lower estimates of N and DM solubility and degradability than FD, the differences being least with silage and FRZ, which was the most effective at rupturing plant structures of the FRS, FMC and FRZ methods. Of the six preparation methods, freeze-drying was the most effective preparation method. FRZ and OVD were less effective, but similar to each other. The small particles (calculated) produced by grinding dried samples may result in an overestimation of zero time losses and hence the degradability of the sample. These undesirable effects might be minimized by coarser grinding (4-mm screen) and correction for particulate matter loss.

Ninety-six Border Leicester × Scottish Blackface ewes, aged 2 to 6 years, grazed perennial ryegrass pastures during lactation. The state of their incisor teeth was assessed at parturition on a scale from score 1 (sound teeth) to score 4 (poor teeth). Their live weights and condition scores during lactation were then related to incisor score and age by regression analysis. Lamb growth was similarly analysed. Herbage intakes and milk production were measured in a subset of 24 ewes.

Ewe live weights were significantly lower, per unit incisor score, by 2·2, 2·5 and 2·1 kg at days 22, 50 and 96 of lactation respectively. In ewes with twin lambs, the equivalent reductions were 1·6, 2·8 and 2·1 kg respectively. Reductions in condition score, whilst still significant, were less marked. After adjusting for the effects of incisor dentition, there was no effect of age on ewe live weight or condition score.

Incisor dentition did not markedly affect herbage intake or milk production, except in measurements at day 53 of lactation, where higher incisor scores resulted in significant reductions in the production of milk and its components. Effects on lamb performance were confined to twins, where live-weight gain to day 50 was reduced by 12·2 g/day, per unit increase in ewe incisor score. This resulted in reductions of 0·7 kg (day 50) and 0·9 kg (weaning) in the live weight of twin lambs.

The effects on herbage intake of changes of herbage mass associated with different stocking rates were studied in Greyface (Border Leicester × Scottish Blackface) ewes in the autumn. Intake was measured in 5-day periods in late September (PI), late October (P2) and late November (P3) on two established, perennial ryegrass/white clover swards (SI and S2). Both swards had previously been variably stocked to produce areas with a range in herbage masses, some of which were grazed continuously (G) at stocking rates declining from 18 to 6 ewes per ha.

Intake was also measured in P2 and P3 on other areas of the swards which were ungrazed for 6 weeks in September and October (C) and then stocked at 18 ewes per ha. Intakes of dry matter, organic matter and digestible organic matter were 16 g, 10 g and 7·5 g/kg live weight, respectively. Organic matter digestibility was 075 in PI and P2 but declined in P3.

Differences in herbage intake and digestibility arose largely because of differences between the swards in herbage mass, sward height and sward density. Ewes in condition scores > 3·00 in early September had lower intakes at all times than did thinner ewes. Changes in sward management associated with different strategies of use had little long-term effect on herbage intake. Keywords: digestibility, herbage, sheep, stocking rate.

Thirty-four mature ewes (17 rearing twins and 17 rearing singles) were used to examine the effect of litter size on herbage intake at pasture by ewes over the course of lactation; intake by their lambs during weeks 13 to 15 of lactation was also evaluated. Daily organic matter intake (OMI) was estimated using the n-alkane technique. Ewes and lambs rotationally grazed 19 × 0·15-ha paddocks during the experimental period which extended from week 2 of lactation to weaning at week 15 of lactation. Sward conditions were maintained steady throughout with daily OM allowance per ewe varying between 4·6 and 5·7 kg and dry matter digestibility between 763 and 858 g/kg DM.

Throughout lactation OMI and OMI per kg body weight were higher for twin-rearing ewes (P < 0·05) than for ewes rearing singles: 2·50 and 2·26 kg/day, and 38 and 32 g/kg per day, respectively. Intake reached a maximum on weeks 6 to 7 of lactation. From weeks 13 to 15 of lactation, OMI and OMI per kg body weight for single- and twin-reared lambs were 1·09 and 1·14 kg/day, and 31 and 36 g/kg per day, respectively.

The combined food intake per dam-offspring unit for weeks 13 to 14 of lactation for a ewe plus her single lamb was 2·8 kg OM per day compared with 4·3 kg OM per day for a ewe plus her two lambs. The repeatability of average weekly intake by ewes was 0·39 over the course of lactation and 0·40 for lambs over the 13 to 15 week period. Repeatability estimates for ewes increased slightly when data from weeks 3 to 7 and weeks 13 to 14 was analysed separately (0·42 and 0·53, respectively).

Continuously grazed rye grassl clover swards with surface heights of 8 to 10 cm at 5 weeks before a synchronized mating and 7·5 cm at mating were grazed by 151 Greyface ewes stocked at 12 per ha until mating. The effects of two stocking rates (eight and 16 ewes per ha) for 6 weeks over the mating and post-mating period were then studied on live weight, body condition and reproductive peformance. Sward height fell more rapidly post mating when stocked at 16 ewes per ha than at eight ewes per ha, but remained above 3·5 cm until 4 weeks after first mating under both rates. Ewes stocked at 16 per ha became significantly lighter and leaner than ewes stocked at eight per ha. There was no significant effect of post-mating stocking rate on reproductive performance in terms of conception rate and lambing rate to first mating or lambing rate to all matings. Reproductive performance of Greyface ewes is therefore unlikely to be adversely affected by post-mating stocking rate on swards of 7 to 8 cm which do not fall below 3·5 to 4·0 cm until 3 to 4 weeks after mating.

Over 4 years, a flock of mature North Country Cheviot ewes was grazed on different swards between weaning in August and mid October to achieve a range of condition scores at 5 weeks before mating (post-weaning period). During the subsequent 5 weeks before a synchronized mating (pre-mating period), all ewes were grazed on a perennial ryegrass/white clover sward, provided at high (H) or low (L) herbage masses (1600 to 2100 or 1200 to 1400 kg dry matter (DM) per ha, respectively). Ovulation rate was derived from the number of corpora lutea in 101 ewes slaughtered after first mating. Conception and lambing rates were derived from the number of lambs born to first mating of 406 ewes. In 2 out of the 4 years, 79 ewes were used to estimate herbage intake.

There was no effect of post-weaning management on reproductive performance. Herbage intake and reproductive performance were examined in relation to the body condition of the ewes at 5 weeks before mating. On an H herbage mass pre-mating, herbage intake in the period immediately prior to mating was greatest in ewes in low body condition and declined significantly with increasing condition. On an L herbage mass pre-mating, herbage intake did not vary significantly with condition. Ovulation rate was low in ewes in low body condition (scores =≤·25) at 5 weeks before mating, increased significantly with increasing condition up to scores 2·50/2·75 but did not change significantly above such intermediate scores. Conception and lambing rates increased significantly with increasing body condition up to scores 2·50/2·75 and then decreased significantly as condition increased above these levels. These effects of body condition were greater on an L herbage mass pre-mating than they were on an H herbage mass. Ewes in the intermediate body condition scores of 2·50 and 2·75 at 5 weeks before mating had a low sensitivity to contemporary nutrition pre-mating. Such condition levels are therefore considered to be above the range of condition in the North Country Cheviot breed within which reproductive response is affected by contemporary nutrition pre-mating.

The lower reproductive performance of ewes in high body condition (scores 3=3·00) at 5 weeks before mating was related to an increase in estimated ova and embryo loss and may be associated with a reduced herbage intake.

The effects on reproductive performance of different strategies of pasture management designed to conserve herbage in situ during the late growing season for use during the pre-mating and mating period were studied in three experiments over 3 years with Greyface ewes. Two experiments with 341 ewes compared set-stocking of pasture at 12 ewes per ha on two initial levels of herbage mass with a conservation strategy which left half of the area ungrazed and grazed the other half of the area at 24 ewes per ha from late August until mid October (2 weeks before the start of mating) after which the total area was grazed at 12 ewes per ha. In a third experiment with 124 ewes, the conservation strategy left a quarter of the area ungrazed and varied stocking rate to a minimum of 12 ewes per ha to maintain two initial levels of herbage mass on the other three-quarters of the area and then compared set-stocking at six ewes per ha on this area with set-stocking at 18 ewes per ha on the previously ungrazed quarter of the area during the pre-mating and mating period.

Initial levels of herbage mass were within the range of 2000 to 2100 kg dry matter (DM) per ha (7 cm sward height; high) and 1500 to 1700 kg DM per ha (4 to 5 cm; low). Mean herbage accumulation rates between August and October were estimated to be 25 and 44 kg DM per ha per day in the first two experiments. Initial live weight and body condition score also varied considerably between years and subsequent response was influenced by herbage growth rate. Where mean ewe body condition was within the score 2·75 to 3·00, range over the mating period and herbage mass on set-stocked areas was not higher than 2200 kg DM per ha (8 cm sward height) in the pre-mating period or not lower than 1300 kg DM per ha (3·5 cm) at about 3 weeks after mating, the strategy of management did not influence reproductive performance in terms of the number of lambs born. Within these limits, however, reproductive performance was positively related to herbage mass in late August (low = 1·60, high = 1·94).

When herbage mass fell below the 1300 kg DM per ha level before or during mating, reproductive performance was improved by a herbage-conservation strategy which maintained ewe body-condition score within the 2·75 to 3·00 range. When herbage mass and growth rate were high, reproductive performance was also improved by a herbage-conservation strategy which restricted the development of excessive body condition before mating by avoiding the increase in barrenness shown to derive from very high levels of body condition.