Hunting, habitat loss and fragmentation have caused a rapid decline in the distribution and abundance of the clouded leopard Neofelis nebulosa across its range, and in several areas, including Taiwan, the species is now extirpated. Taiwan, a former stronghold for the species, is a candidate for its reintroduction, based on increasing prey abundance and high forest coverage. Such future reintroduction efforts, however, are hampered by a lack of analysis of potential clouded leopard habitat on the island. To address this, we explore habitat suitability for the species in Taiwan. We used a multi-scale, multivariate habitat selection model based on clouded leopard presence–absence data from extensive camera-trap surveys across its current range to predict suitable habitat in Taiwan. Our findings indicate that 38% of Taiwanese territory is potentially suitable habitat for the clouded leopard, of which 46% is under protection. This demonstrates the high potential of Taiwan's habitat for clouded leopard reintroduction.

Epilobium belongs to the family Onagraceae, which consists of approximately 200 species distributed worldwide, and some species have been used as medicinal plants. Epilobium nankotaizanense is an endemic and endangered herb that grows in the high mountains in Taiwan at an elevation of more than 3300 m. Alpine herbs are severely threatened by climate change, which leads to a reduction in their habitats and population sizes. However, only a few studies have addressed genetic diversity and population genetics. In the present study, we developed a new set of microsatellite markers for E. nankotaizanense using high-throughput genome sequencing data. Twenty polymorphic microsatellite markers were developed and tested on 30 individuals collected from three natural populations. These loci were successfully amplified, and polymorphisms were observed in E. nankotaizanense. The number of alleles per locus (A) ranged from 2.000 to 3.000, and the observed (Ho) and expected (He) heterozygosities ranged from 0.000 to 0.929 and from 0.034 to 0.631, respectively. The developed polymorphic microsatellite markers will be useful in future conservation genetic studies of E. nankotaizanense as well as for developing an effective conservation strategy for this species and facilitating germplasm collections and sustainable utilization of other Epilobium species.

Darwin's frogs Rhinoderma darwinii and Rhinoderma rufum are the only known species of amphibians in which males brood their offspring in their vocal sacs. We propose these frogs as flagship species for the conservation of the Austral temperate forests of Chile and Argentina. This recommendation forms part of the vision of the Binational Conservation Strategy for Darwin's Frogs, which was launched in 2018. The strategy is a conservation initiative led by the IUCN SSC Amphibian Specialist Group, which in 2017 convened 30 governmental, non-profit and private organizations from Chile, Argentina and elsewhere. Darwin's frogs are iconic examples of the global amphibian conservation crisis: R. rufum is categorized as Critically Endangered (Possibly Extinct) on the IUCN Red List, and R. darwinii as Endangered. Here we articulate the conservation planning process that led to the development of the conservation strategy for these species and present its main findings and recommendations. Using an evidence-based approach, the Binational Conservation Strategy for Darwin's Frogs contains a comprehensive status review of Rhinoderma spp., including critical threat analyses, and proposes 39 prioritized conservation actions. Its goal is that by 2028, key information gaps on Rhinoderma spp. will be filled, the main threats to these species will be reduced, and financial, legal and societal support will have been achieved. The strategy is a multi-disciplinary, transnational endeavour aimed at ensuring the long-term viability of these unique frogs and their particular habitat.

Cinnamomum chago is a woody species of the family Lauraceae endemic to Yunnan province, China, previously known from only one location, and categorized as a Plant Species with Extremely Small Population. We surveyed to determine the distribution and population size of C. chago, characterize its habitat, identify any threats, assess its conservation status, and provide guidelines for its management and conservation. During 2014–2017 we found only 64 mature C. chago, in five locations. These small, fragmented populations occur along Lancang River in Dali Prefecture at altitudes of 2,200–2,400 m. The species' extent of occurrence is c. 923 km2, with an area of occupancy of c. 60 km2. The habitat of the species has been degraded by expansion of pastoral activities and deforestation. We recommend categorization of C. chago as Endangered on the IUCN Red List, prevention of the collection of seeds and wood of the species, protection and monitoring, and ex situ propagation for future reintroductions.

Wenchengia alternifolia (Lamiaceae) is a plant species endemic to Hainan Island, China. Since the 1930s it was known from only four collections and was believed to be extinct until a remnant population was rediscovered in 2010. We conducted further field surveys during 2010–2012 but located only one population, with 66 individuals. W. alternifolia is restricted to the c. 1,500 m2 Shuangximu Valley, in a harsh microenvironment surrounded by plantations. As the population comprises < 50 mature individuals, we propose that W. alternifolia be categorized as Critically Endangered on the IUCN Red List, based on criterion D. We recommend that the remnant population and its habitat need urgent protection and monitoring and that ex situ conservation, for future reintroduction, should be implemented.

Relict species provide a unique opportunity to understand past and recent biogeographical and evolutionary processes. Zelkova abelicea (Ulmaceae), which is endemic to the island of Crete (Greece), is one of the most prominent Tertiary relict trees of the Mediterranean region. We collected distribution, threat and population structure data by reviewing literature and herbaria and through field surveys at 14 study plots throughout the range of the species. The present distribution of Z. abelicea is extremely fragmented. Although the total estimated number of individuals is relatively high, the populations are dominated by dwarf, severely browsed, non-flowering individuals. The population structure is asymmetric. At most, 5% of a plot's trees are large and fruit-bearing. The asymmetric structure is particularly pronounced in isolated and small populations. Based on its limited geographical range, the fragmented spatial pattern, and data on distribution and population structure, our study confirms that Z. abelicea is a threatened species (IUCN category Endangered). Our research aim is to promote the development of new approaches for the improvement of conservation strategies for Tertiary relict trees characterized by major local disjunctions.

This paper presents a study to partition the role of three regional-scale drivers – woodland extent and continuity, pollution regime, and climatic setting – in explaining the composition and richness of lichen epiphytes in Scotland. To do this we used partial canonical correspondence analysis and multiple least squares regression, to examine lichen communities across 170 study sites. First, our results demonstrate the importance of climate in explaining species composition. This highlights the relatively clean-air environment of Scotland within a European setting, and emphasizes the important consideration of regional context in the development of bioclimatic species-response models. This result contrasts with a previous similar study which collapsed complex environmental data into summary gradients, and which therefore discounted climate as a key factor. Second, we show a functional decoupling between composition and species richness, which was optimally explained by old-growth woodland extent and pollution, and only weakly explained by climate. The difference in explanatory variables between composition and richness is a focal issue in determining the processes by which species compositional change, driven by rapid and deep climate change, may indirectly impact species richness. For example, this impact may occur through an imbalance in rates of species extinction (for sensitive range-edge species) and establishment in a fragmented landscape (for dispersal-limited colonists), though operating against the ‘stabilizing effect’ of microclimatic setting.

The dry tropical ecosystems are among the world's most threatened, and the dry deciduous forest of northern India is being progressively converted to scrub, savannah and grasslands through industrialization, agriculture, fuelwood collection, lopping of trees for fodder and severe grazing/browsing. This habitat destruction threatens the survival of many species. This study examined the demographic instability of tree species in 3-ha permanent plots: at five sites differing in the degree of disturbance. Based on the proportion of seedlings of a species in its total population (seedling + sapling + adults), about 52% of the total 65 species exhibited local demographic instability, and at one or more sites a single individual represented 10 species. The increase in the proportion of declining species with increase in disturbance intensity indicated that local anthropogenic pressure is responsible for the depletion. Apart from stronger protection measures, it is necessary to encourage fuelwood plantations, develop village pastures and reduce livestock numbers.

Wilderness designation continues to be a contentious effort and must be fully justified even in wealthy countries such as the USA. An important consideration in setting priorities for additional designations of Wilderness is to ensure that under-represented ecosystems are protected. The utility of Geographic Information Systems in performing this task is illustrated using data on ecoregions and areas in the National Wilderness Preservation System to determine the relative protection currently afforded to different ecoregions in the continental USA. We find that 23 of the 35 ecoregions have less than 1% of their land area protected as Wilderness, and 7 of the 35 have no land protected as Wilderness whatsoever. While much of the land with little protection is in areas dominated by private land ownership in the mid-west and southeast, a surprisingly large amount of land in the Intermountain states of Nevada and Utah, which is in public ownership, is substantially under-represented in the National Wilderness Preservation System as well. The implications of this analysis for wilderness allocation strategies are detailed. The technique illustrated in this paper is a useful aid in designing protected area strategies in countries throughout the world.