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amine-organic buffers

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1 results

Freezing buck semen diluted in amine-organic buffers
S. Dunner
Journal:

Animal Production / Volume 56 / Issue 3 / June 1993

Published online by Cambridge University Press:

02 September 2010, pp. 387-391

Print publication:

June 1993
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Amine-organic buffers (BESKOH and TESTRIS) were compared for their ability to be used for buck semen dilution in a freezing procedure. BESKOH showed better results (P < 0·05) at the time of dilution and after chilling at +5°C (72% motility v. 54% at dilution and 62% v. 39% after chilling). After freezing and thawing, none of the variables measured (motility, normal acrosome ridge and normal swelling) was significantly different. A washing procedure was necessary when freezing was undertaken and egg yolk addition was necessary to avoid cold shock as the temperature lowered during chilling. There were no significant differences between a 3% and 12% level of egg yolk addition.