This study investigates the genetic basis of lamb vigour (defined as neonatal lamb activity and sucking ability) and lambing difficulty as potential traits to be included in selection programmes to improve ewe and lamb welfare. Scores for lamb birth difficulty, vigour and sucking ability were collected shortly after birth on 1,520 lambs born in 2006 in 19 different flocks that were members of the UK Suffolk Sire Referencing Scheme. Scores evaluated each trait on a scale of 1 to 4; 1 being no assistance given either during birth or to suck, or excellent vigour, through to 4 where a large degree of assistance was required, or poor vigour. Genetic parameters (heritabilities, genetic correlations) were estimated by fitting an individual animal model using ASREML. Variance components obtained from univariate and bivariate analyses were averaged to provide genetic parameter estimates. Heritabilities for birth difficulty and vigour were moderate but heritability for sucking ability was not significant. The genetic correlation between vigour and sucking ability was positive and high, that between vigour and birth difficulty moderately negative, and that between birth difficulty and sucking ability not significant. Birth difficulty and vigour could be included in Suffolk breeding programmes to help reduce health and welfare problems associated with these traits in Suffolk sheep, and in flocks producing crossbred lambs sired by Suffolk rams. Further work is required to evaluate correlations between these traits and performance traits and to comprehensively validate the scoring system once more data become available.

Rapid, non-destructive methods for measuring seed germination and vigour are valuable. Standard germination and seed vigour were determined using 81 soybean seed lots. From these data, seed lots were separated into high and low germinating seed lots as well as high, medium and low vigour seed lots. Near-infrared spectra (950–1650 nm) were collected for training and validation samples for each seed category and used to create partial least squares (PLS) prediction models. For both germination and vigour, qualitative models provided better discrimination of high and low performing seed lots compared with quantitative models. The qualitative germination prediction models correctly identified low and high germination seed lots with an accuracy between 85.7 and 89.7%. For seed vigour, qualitative predictions for the 3-category (low, medium and high vigour) models could not adequately separate high and medium vigour seeds. However, the 2-category (low, medium plus high vigour) prediction models could correctly identify low vigour seed lots between 80 and 100% and the medium plus high vigour seed lots between 96.3 and 96.6%. To our knowledge, the current study is the first to provide near-infrared spectroscopy (NIRS)-based predictive models using agronomically meaningful cut-offs for standard germination and vigour on a commercial scale using over 80 seed lots.

Obtaining corn hybrid seeds (Zea mays L.) with high vigour depends on the parental lines and the direction of the cross, and this relates to seed desiccation tolerance and composition. This research studied reciprocal crosses between pairs of proprietary, elite parent lines (L1 and L5; L2 and L4) producing hybrid seeds with different qualities attempting to correlate vigour with seed composition, focusing on storage proteins, starch and soluble sugar amounts. Four corn hybrid seed lots produced from reciprocal crosses were compared (HS 15 with HS 51, and HS 24 with HS 42) by assessing germination, vigour, and seedling emergence in the field. Seed composition was assessed in mature, dehydrated seeds. Proteins were extracted, quantified, and analysed by electrophoresis and densitometry. Starch amounts were assessed using a kit and soluble sugars were determined using high performance liquid chromatography with pulsed electrochemical detection. The L1 and L2 lineages, used as female parents, provided seeds with lower vigour; however, the quantification of major protein bands, and sucrose, raffinose and stachyose were similar between seed lot pairs. While both total seed protein and starch varied between reciprocal hybrids for one of the two sets of crosses, the amounts of neither correlated with seed vigour. Interestingly, hybrids with low seed vigour (HS 15, HS 24) accumulated greater amounts of fructose relative to their reciprocal; correlation analysis confirmed these results. We demonstrate different effects on seed vigour dependent on the maternal parent in reciprocal crosses producing hybrid corn seeds. We also show that vigour is negatively correlated with seed reducing sugar contents.

Supporting the application of positive psychology in the workplace, the Shirom Melamed Vigor Scale (SMVS), which assesses vigorous feelings at work, was adapted to the Thai context. The Thai SMVS contains 14 items as in the original scale and was validated using a sample of 585 employed participants in Thailand. Results of a confirmatory factor analysis are consistent with the conceptual model of vigour as consisting of physical strength, cognitive liveliness, and emotional energy, with a suggestion that a 13-item scale can also be used. As expected, vigour has been found to be conceptually distinct from some similar constructs, and independent from job burnout. Vigour also showed unique predictive effects on employees’ health. These results support the previously-proposed conceptual framework of vigour and indicate crucial roles of positive emotion in the work setting. In concordance with a positive organisational psychology approach, applications of the Thai vigor scale are discussed with respect to its potential for enhancing employees’ wellbeing and productivity.

Seed germination is responsive to diverse environmental, hormonal and chemical signals. Germination rates (i.e. speed and distribution in time) reveal information about timing, uniformity and extent of germination in seed populations and are sensitive indicators of seed vigour and stress tolerance. Population-based threshold (PBT) models have been applied to describe germination responses to temperature, water potential, hormones, ageing and oxygen. However, obtaining detailed data on germination rates of seed populations requires repeated observations at frequent times to construct germination time courses, which is labour intensive and often impractical. Recently, instruments have been developed to measure repeatedly the respiration (oxygen consumption) of individual seeds following imbibition, providing complete respiratory time courses for populations of individual seeds in an automated manner. In this study, we demonstrate a new approach that enables the use of single-seed respiratory data, rather than germination data, to characterize the responses of seed populations to diverse conditions. We applied PBT models to single-seed respiratory data and compared the results to similar analyses of germination time courses. We found consistent and quantitatively comparable relationships between seed respiratory and germination patterns in response to temperature, water potential, abscisic acid, gibberellin, respiratory inhibitors, ageing and priming. This close correspondence between seed respiration and germination time courses enables the use of semi-automated respiratory measurements to assess seed vigour and quality parameters. It also raises intriguing questions about the fundamental relationship between the respiratory capacities of seeds and the rates at which they proceed toward completion of germination.

Introduction. Mango productivityis low in seasonally wet-dry tropical areas where breeding programsrequire information on factors affecting productivity of mango cultivars.Specifically, our study tested a novel hypothesis that, among Australian-and Florida-bred cultivars, the greater growth of vegetatively vigorouscultivars would contribute to lower levels of fruit production in comparisonwith less vegetatively vigorous cultivars, in a wet-dry tropical environment. Materials and methods. A field experiment was conductedon trees of the cultivars ‘Kensington Pride’ and ‘Strawberry’, both polyembryoniccultivars, and ‘Haden’, ‘Irwin’ and ‘Tommy Atkins’, all monoembryoniccultivars. Results. Shoot growth was recorded overtwo years; in both years the polyembryonic cultivars produced morenew shoot length than the monoembryonic cultivars; ‘Irwin’ was theleast vigorous cultivar in both years. Across cultivars, there wasa negative relationship between normalised (by flowering intensity andcanopy area) fruit number or yield and vegetative vigour as representedby new shoot length. Conclusion. The results supportedthe hypothesis that the greater shoot growth of vegetatively vigorouscultivars contributed to lower levels of fruit production in comparisonwith less vegetatively vigorous cultivars in a tropical environment.This is the first study which demonstrates that the extent of seasonalshoot growth had a fruit production cost for mango.

Alfalfa (Medicago sativa L.) yield is severely compromised by soil salinity, especially at the level of seedling establishment. This question was addressed by proteomics to decipher whether specific changes in protein accumulation correlate with germination performance of alfalfa seeds submitted to a salinity stress as obtained by imbibing seeds in the presence of NaCl. This study used alfalfa seeds submitted to an osmopriming invigoration treatment that proved very efficient in counteracting the negative effect of salinity stress on germination performance. Comparative proteomic analyses disclosed 94 proteins commonly characterizing the response of both the untreated control and osmoprimed seeds to the experimental salinity stress. Remarkably, many of them, representing 84 proteins, showed contrasting accumulation patterns when comparing the untreated control and osmoprimed seeds submitted to the same salt stress. Thus numerous changes observed in the proteome of the untreated control seeds imbibed in the presence of salt, and presumably accounting for the loss in seed vigour associated with salinity stress, can be substantially reversed in osmoprimed seeds undergoing this stress. These data therefore provide a biochemical understanding of the increase in seed vigour generally observed with primed seeds.

In a sample of 1449 adults, divided by age into young, middle-age, and old, mood was found to consist of two age invariant components, vigour and affect. Factor structure differed by age for affect but not vigour. For old adults, two unipolar affect factors were obtained, whereas one bipolar affect factor was obtained for the two younger groups. From these factor analyses a mood scale (the Memorial University Mood Scale, the MUMS) was developed and its predictive validity and reliability established for all age groups. Using the MUMS, mean level differences by age were found in both vigour and affect, as well as a measure of globed mood, with the old adults higher on all three measures. Age invariant, diurnal patterns were found for both vigour and affect. Vigour followed an inverted U-shaped diurnal pattern and affect a primarily linear pattern, suggestive of appraisals of somatic state and environmental conditions, respectively.

The effectiveness of ATPase in germinated seed may play an important role in the vigour of germination. The activities of tonoplast and plasma membrane ATPases in two maize (Zea mays L.) lines with different vigour of germination were determined. ATP hydrolysis was measured in microsomal fractions from coleoptiles along with the responses to specific inhibitors for the plasma membrane, tonoplast and mitochondrial ATPases as well as for acid phosphatase. Nitrate-sensitive ATPase activity was 1.5–3.0 times lower in the low-vigour line than in the high-vigour line. Kinetic analysis of ATP hydrolysis at different substrate concentrations revealed the existence of two enzymes in the microsomal fractions of the two lines. The Vmax of enzyme 1 in the low-vigour line was a third of that in the high-vigour line. This enzyme was identified as the nitrate-sensitive or tonoplast ATPase on the basis of measurements of ATP hydrolysis in the presence of specific inhibitors at high (8.12mm) and low (0.77mm) ATP concentrations.

Even though the embryo of a maize (Zea mays L.) kernel contributes relatively little to total kernel weight, it is a main source of electrolytes which leach from the kernel during imbibition. Ageing of maize kernels for 18 days at 40°C and a moisture content of about 15% results in an increase of electrolyte leakage which almost exclusively originates from the embryo. The effect of ageing is most apparent after prolonged periods of imbibition. Mechanical damage increases leakage early during imbibition, the effect of damage being considerably larger for aged than for unaged kernels. The large amount of electrolytes measured after the first hour of imbibition of undamaged kernels comes mainly from the pericarp. The electrolyte content of the pericarp is variety dependent and may interfere with quality testing by conductivity measurements.

Growers expect high-quality, genetically pure seed. As a result, seed companies maintain quality control programmes that monitor seed from harvest to purchase. An array of ‘traditional’ seed quality tests, including mechanical tests and tests of genetic purity, seed germination and vigour, and seed health tests, is used, and seed quality assessment techniques continue to evolve. Advances in molecular genetics are allowing the release of new varieties differing essentially in one gene. New molecular biology approaches offer the potential to identify these subtle genetic differences. Advances in seed enhancements, such as pelleting, priming and pregermination, require increased scrutiny of seed quality before and after the enhancement process. New developments in computer imaging for improved purity and germination/vigour analyses are being developed. These novel approaches to seed quality assessment become important as new genetic improvements are conveyed in the seed at increased cost to the grower.

The loss of percentage germination and vigour of non-sprouted and sprouted seeds of winter wheat was determined during storage for 80 days at 11 different combinations of moisture content and temperature. Before storage the initial percentage germination and vigour were reduced according to the level of sprouting. During storage the percentage germination and vigour decreased. The rate of germination loss increased with increasing seed moisture content and/or increasing temperature. The species-specific constants KE, CW, CH and CQ of the viability equation were not different for non-sprouted and sprouted seeds. Likewise, the relation between the percentage of normal and abnormal seedlings was independent of sprouting. The rate of vigour loss was also independent of the storage conditions and of sprouting.

To determine whether feeding a sustainable, algal source of docosahexaenoic acid (DHA) to sheep during late pregnancy would improve neonatal lamb vigour, 48 English mule ewes, of known conception date, were divided into four treatment groups. For the last 9 weeks of gestation, ewes received one of two dietary supplements: either a DHA-rich algal biomass providing 12 g DHA/ewe per day, or a control supplement based on vegetable oil. The four dietary treatment groups (n = 12) were: control supplement for the duration of the trial (C), DHA supplement from 9 to 6 weeks before parturition (3 week), DHA supplement from 9 to 3 weeks before parturition (6 week) and DHA supplement for the duration of the trial (9 week). Dietary supplements were fed alongside grass silage and commercial concentrate. There was a tendency for gestation length to be extended with increased duration of DHA supplementation (P = 0.08). After parturition, the concentrations of eicosapentaenoic acid (EPA) and DHA in ewe and lamb plasma and colostrum were elevated in line with increased periods of DHA supplementation. Lambs from the 6-week and 9-week groups stood significantly sooner after birth than lambs from the C group (P < 0.05). These data show that neonatal vigour may be improved by the supplementation of maternal diets with DHA-rich algal biomass and that this beneficial effect depends upon the timing and/or duration of DHA allocation.

Introduction. Research was conducted at the Pistachio Research Institute in Rafsanjan, Iran, to evaluate the effects of ‘Badami’ (Pistacia vera L.), ‘Sarakhs’ (wild P. vera) and ‘Beneh’ (P. mutica F. & M.) seedling rootstocks on the nut quality of ‘Owhadi’, ‘Kalleh-ghuochi’ and ‘Ahmad-aghaie’ pistachio cultivars. Twenty-year-old trees of these nine rootstock/scion combinations were used in a two-year study done in 2003 and 2004. Materials and methods. Growth parameters [trunk cross-sectional area (TCA), tree height, canopy diameter, shoot growth, leaf area and number of suckers], yield parameters (cumulative yield and yield efficiency), and nut quality (blankness, splitting, nuts per ounce and weight of 100 nuts) were measured. Results and discussion. TCA was significantly lower on ‘Sarakhs’ rootstock than on the two other rootstocks and ‘Sarakhs’ was the least vigorous among the tested rootstocks and gave the lowest leaf area of scion cultivars. ‘Sarakhs’ rootstock consistently influenced the formation of the highest number of suckers. ‘Badami’ rootstock allowed us to obtain the greatest cumulative production and yield efficiency, the lowest blankness of pistachio nuts, and the highest percentage of splitting of pistachio nuts. Conclusion. Our study showed that genetic variability among pistachio rootstocks may influence scion vigour, yield, extent of shell splitting, blankness and nut weight.

Internal cracks caused by high temperature or excessive moisture during maize (Zea mays L.) kernel development were characterized, and their effects on kernel quality were assessed. Pre-harvest stress cracks are often located near the middle of the kernel along the embryo axis, but they were also detected in other positions, irrespective of the shape of the kernel. X-ray analysis enabled visualisation of stress cracks that are invisible to the human eye and, therefore, gave a better estimate of the percentage of cracks. However, low temperature scanning electron microscopy of the surface of milled kernels revealed small cracks not noticed by visual or X-ray inspection. All kernels tested in this way had a crack of some sort in the endosperm tissue. Cracks were also frequent in the scutellum, but rare in the embryo axis. Endosperm cracks followed the boundary of the starch granules, but did not extend into the pericarp tissue. In contrast to external cracks caused by mechanical impact, pre-harvest internal stress cracks generally are not detrimental to germination and vigour. However, if the crack is located inside or perpendicular to the embryo axis, it may affect the quality of the kernel, probably by impeding nutrient translocation to the embryo.

An image analysis system is described to study the process of imbibition in white cabbage (Brassica oleracea L.) seeds. The system consists of two components: 1) an environmental system where seeds germinate; 2) a computer imaging system, composed of a Charged Coupled Device (CCD)-imaging sensor, an image frame grabber, a computer and a video monitor. The measurement software can determine area, perimeter, width, length and eccentricity as descriptors of changes in seed size during swelling. The captured images of imbibing seeds allowed estimation of timing of ‘visible germination’ occurrence without any subjective evaluation. Using the area parameter, the imbibition process can be described graphically by a series of curves similar to the triphasic pattern of water uptake, with extension and rate depending on the degree of seed viability and germination medium conditions. Imbibitional osmotic stress, provided by -1.5 MPa mannitol, induced a large delay in germination and, upon stress removal, a more uniform radicle emergence. The versatility and the sensitivity of the method, together with the feasibility to investigate germination rate in individual seeds within a population, suggest that image analysis techniques have high potential in seed biology studies.

The aim of the present investigation was, to examine whether outdoor wintering of lambing ewes is a successful and animal friendly production system. The welfare and growth performance of winterborn lambs in outdoor husbandry were assessed in two winter periods (1995/96, 1996/97) when a total of 353 lambs were born into three different husbandry systems. System 1 consisted of 75 lambs from 26 (winter 1) and 29 (winter 2) ewes and was wintered outdoors with access to a lambing shelter with a straw bedded floor. System 2 included 120 lambs from 52 (winter 1) and 54 (winter 2) ewes which were born in an uninsulated barn with access to an outdoor yard. System 3 was kept in an uninsulated barn with no access to the outside and consisted of 158 lambs from 52 (winter 1) and 54 (winter 2) ewes. The lambs were of five different genetic origins which were equally distributed over the three husbandry systems. The genetic groups included purebreds from the hardy ‘Rhönschaf’ (RHO) and from the ‘German Blackface’ (GBF) mutton breed and the reciprocal crosses of these breeds (GBF ✕ RHO, RHO ✕ GBF). The fifth group were crossbreds between rams of the small-framed French mutton breed ‘Charmoise’ (CHA) and Rhönschaf-ewes (CHA ✕ RHO). The time period between parturition and first standing (‘time to stand’) and the time period from birth to first sucking (‘time to suck’) were considered as vigour traits. Vigour was visually assessed and scored. The rectal temperature was measured 3 h post partum. Live-weight gains were estimated from birth to 25 and 42 days of life. No differences between the different husbandry systems were observed in the measured traits. Outdoor-born lambs showed the same vigour and were able to maintain homeothermy as well as those born indoors. Ewes sought the shelter prior to lambing which might have favoured the early vigour of lambs and thus, their ability to maintain homeothermy. The visually assessed vigour score was in good accordance with the vigour traits time to stand and time to suck. The breed of the ewe had a significant effect on lamb vigour, lambs from hardy RHO ewes showing a better vigour than lambs from GBF ewes. Hybrid vigour estimates of time to stand and time to suck were 0·17 and 0·20, respectively, but were not statistically significant.

Crossbred lambs between GBF and CHA mutton rams and hardy RHO ewes had a good vitality and growth performance. Outdoor wintering with a lambing shelter did not adversely influence survival of newborn lambs or their subsequent growth performance.