The tolerance of cereal rye to eight herbicides registered for use in wheat, at two rates, was evaluated for potential labeling in cereal rye to expand limited chemical weed control options. Across five site-years, halauxifen-methyl + florasulam, pyroxsulam, and thifensulfuron-methyl + tribenuron-methyl applied at a 2X rate to cereal rye at Zadoks (Z) 13 caused less than 15% injury and had no impact on cereal rye density. These herbicides at the 2X rate reduced cereal rye heights 11% at 10 days after treatment (DAT), with rye recovering by 31 DAT; cereal rye heights were not reduced with these herbicides at their 1X rate. In contrast, significant injury was observed with the 1X rate of mesosulfuron-methyl (45%), pinoxaden (27%), and pinoxaden + fenoxaprop-P-ethyl (30%) applied postemergence; early-season height was reduced 19% to 26%. Residual herbicide pyroxasulfone applied as a delayed preemergence at Z 10 and flumioxazin + pyroxasulfone applied at Z 11 caused 27% to 28% and 16% to 47% injury, respectively, when the 1X rate was activated by rainfall within 2 d of application. These residual herbicides reduced cereal rye height and density up to 35% and 40%, respectively. Cereal rye grain yield was not influenced by herbicide or rate applied.

Catchweed bedstraw (Galium aparine L.) is a problematic dicot weed that occurs in major winter wheat (Triticum aestivum L.) fields in China. Tribenuron-methyl has been widely used to control broadleaf weeds since 1988 in China. However, overuse has led to the resistance evolution of G. aparine to tribenuron-methyl. In this study, 20 G. aparine populations collected from Shandong and Henan provinces were used to determine tribenuron-methyl resistance and target-site resistance mechanisms. In dose–response experiments, 12 G. aparine populations showed different resistance levels (2.92 to 842.41-fold) to tribenuron-methyl compared with the susceptible population. Five different acetolactate synthase (ALS) mutations (Pro-197-Leu, Pro-197-Ser, Pro-197-His, Asp-376-Glu, and Trp-574-Leu) were detected in different resistant populations. Individuals heterozygous for Pro-197-Ser and Trp-574-Leu mutations were also observed in a resistant population (HN6). In addition, pHB4 (Pro-197-Ser), pHB7 (Pro-197-His), pHB8 (Pro-197-Leu), pHB5 (Asp-376-Glu), and pHB3 (Trp-574-Leu) subpopulations individually homozygous for specific ALS mutations were generated to evaluate the cross-resistance to ALS-inhibiting herbicides. The pHB4, pHB7, pHB8, pHB5, and pHB3 subpopulations all were resistant to sulfonylurea, pyrazosulfuron-ethyl, triazolopyrimidine, flumetsulam, sulfonylamino-carbonyl-triazolinone, flucarbazone-sodium, pyrimidinyl thiobenzoate, pyribenzoxim, and the imidazolinone imazethapyr. These results indicated the diversity of the resistance-conferring ALS mutations in G. aparine, and all these mutations resulted in broad cross-resistance to five kinds of ALS-inhibiting herbicides.

Eclipta, widespread in tropical, subtropical, and temperate regions, is one of the main malignant broadleaf weeds and thrives in moist and dryland fields. Field rates of acetolactate synthase (ALS) inhibitors have failed to control eclipta in some farmlands in China. One ALS inhibitor–resistant population (R) collected from Jiangsu province in China was confirmed in the greenhouse in our preliminary work. Whole-plant assays revealed that this R population was highly resistant to four sulfonylureas (pyrazosulfuron-ethyl, 134-fold; bensulfuron-methyl, 172-fold; metsulfuron-methyl, 30-fold; and tribenuron-methyl, 195-fold), two triazolopyrimidines (pyroxsulam, 98-fold; penoxsulam, 30-fold), and one pyrimidinylthio-benzoate (bispyribac-sodium, 166-fold) and was moderately resistant to two imidazolinones (imazethapyr, 10-fold; imazapic, 19-fold). ALS enzyme-activity assays showed insensitivity of the ALS from the R population (resistance index values ranged from 12 to 293) to all of the above ALS inhibitors in vitro. Chromatograms from ALS gene sequence analysis detected a homozygous Pro-197-Ser amino acid substitution in the R population. These results confirmed that the Pro-197-Ser substitution results in broad-spectrum cross-resistance to ALS inhibitors in the eclipta R population. To our knowledge, this study is the first to report broad cross-resistance to ALS inhibitors in eclipta and to obtain the full-length ALS gene sequence.

Japanese bindweed was found to be one of the most abundant and most difficult-to-control weed species during a 2-yr weed survey in more than 100 winter wheat fields in the North China Plain region. Multivariate data analysis showed that Japanese bindweed is most abundant at sites with comparative low nitrogen (N) fertilization intensities and low crop densities. To gain deeper insights into the biology of Japanese bindweed under various N fertilization intensities, winter wheat seeding rates, herbicide treatments, and their interactions, a 2-yr field experiment was performed. In nonfertilized plots, a herbicide efficacy (based on density reduction) of 22% for 2,4-D, and of 25% for tribenuron-methyl was found. The maximum herbicide efficacy in Nmin-fertilized plots (target N value based on expected crop yield minus soil mineral nitrogen content, ) was 32% for 2,4-D and 34% for tribenuron-methyl. In plots fertilized according to the farmer's practices, a maximum herbicide efficacy of 72% for 2,4-D and of 64% for tribenuron-methyl could be observed. Furthermore, medium and high seeding rates improved the herbicide efficacy by at least 39% for tribenuron-methyl and 44% for 2,4-D compared to the low seeding rate. Winter wheat yield was not significantly affected by seeding rate itself, whereas at low and medium seeding rates, Nmin fertilization was decreasing winter wheat yield significantly compared to the farmer's usual fertilization practice. At the highest seeding rate, Nmin fertilization resulted in equal yields compared to the farmer's practices of fertilization.

A field experiment was conducted in 2009–2010 at Pennsylvania and Maryland locations, and repeated it in 2010–2011 to test the effectiveness of POST-applied herbicides at fall and spring timings on seeded hairy vetch in winter wheat. A total of 16 herbicide treatment combinations was tested that included synthetic auxins, acetolactate synthase (ALS) inhibitors, and a protoporphyrinogen oxidase inhibitor. Spring applications tended to be more effective than fall applications. Among synthetic auxins, clopyralid (105 g ae ha−1) and treatments containing dicamba (140 g ae ha−1) were effective at both timings, resulting in greater than 90% hairy vetch control at wheat harvest. Pyroxsulam and prosulfuron applied at 18 g ai ha−1 provided the most effective hairy vetch control (> 90%) at both application timings among ALS inhibitors. Spring applications of several herbicides provided moderate (> 80%) to high (> 90%) levels of hairy vetch control, including: 2,4-D amine (140 g ae ha−1), mesosulfuron-methyl (15 g ai ha−1), tribenuron-methyl (13 g ai ha−1), and thifensulfuron/tribenuron-methyl treatments (16 and 32 g ai ha−1). Winter wheat injury was evaluated, but symptoms were negligible for most treatments. Winter wheat yields declined with increasing hairy vetch biomass. Fall herbicides may be prioritized to reduce hairy vetch competition during the fall and early spring growing season. Our research has established that several synthetic auxin and ALS-inhibiting herbicides, applied POST in fall or spring, can be safely used in winter wheat to control hairy vetch in an integrated weed management program.

The molecular basis of resistance to tribenuron-methyl, an acetolactate synthase (ALS)–inhibiting herbicide was investigated in four resistant (R) and three susceptible (S) flixweed populations. The resistance level in the R populations was assessed in whole-plant pot experiments in a greenhouse, and resistance indices ranged from 723 to 1422. The ALS genes of the three S populations and four R populations were cloned and sequenced, and the full coding sequence of the ALS gene of flixweed was 2,004 bp. The sequences of the ALS genes of the three S populations collected from Shaanxi, Gansu, and Tianjin were identical. Comparison of the ALS gene sequences of the S and R populations with Arabidopsis revealed that proline at position 197 of the ALS gene was substituted by leucine in R population SSX-2, by alanine in R population SSX-3, and by serine in R populations TJ-2 and GS-2. In another study of two R flixweed populations from Hebei and Shaanxi, resistance was also related to mutation at position 197 of the ALS gene. Both studies confirmed tribenuron-methyl resistance in flixweed in China, with the resistance mechanism being conferred by specific ALS point mutations at amino acid position 197.

Broadleaf weeds are common and troublesome pests in cool-season turfgrass species such as tall fescue, Kentucky bluegrass, perennial ryegrass, and creeping bentgrass. Broadleaf weeds are primarily managed in these grasses through POST applications of growth regulator herbicides in the phenoxy, benzoic acid, and pyridine chemical classes. There are disadvantages to use of these chemicals, including nontarget plant damage and limited residual control. Certain annual broadleaf weeds can be controlled through application of isoxaben or a PRE crabgrass herbicide, but these herbicides do not control emerged broadleaf weeds. There are advantages to use of sulfonylurea herbicides, including PRE and POST control of annual and perennial weeds, a different mode of action, and these herbicides have low vapor pressure, reducing the potential for offsite movement. There are disadvantages to the use of sulfonylurea herbicides, including limited spectrum of broadleaf weed species controlled and limited tolerance in cool-season turfgrass species. The primary sulfonylurea herbicides used in cool-season turfgrass are chlorsulfuron, halosulfuron, metsulfuron, and sulfosulfuron. There have been specialized uses for primisulfuron and tribenuron-methyl.

Today, the aim of weed management is to keep the weed community at an acceptable level rather than to keep the crop totally free of weeds. Satisfactory control of weeds may often be obtained when herbicides are used at lower doses than normally recommended. To facilitate the decision of what is an adequate dose in a specific field, the farmer needs support. In 1988 and 1989, a total of 10 field trials in spring cereals were initiated in Sweden with the objective of studying long-term effects of herbicide application according to recommendations from guidelines: the guidelines were developed at the Swedish University of Agricultural Sciences and consisted of printed cards designed for in-field use. Treatments also included a full and a half dose and an untreated control. As an average over the experimental time, i.e., until 1997, the dose used in the guideline treatment varied at different sites between 20 and 70% of a full dose. In 1998, i.e., 1 yr after the last herbicide application, the plant densities of annual weeds in the guideline treatment, the half and the full doses were 51, 57, and 67% lower, respectively, than in the untreated control when averaged over sites. At two and four sites, the half and full doses resulted in significantly lower weed densities than where guidelines had been used. Compared with the control, the full and half doses increased the proportion of difficult-to-control weed species significantly at five and four sites by 21 and 24%, respectively. In the guideline treatment the proportion of difficult-to-control weeds was increased at one site. In 1998, weed counts were higher where guidelines had been used than in the full dose for common lambsquarters and common chickweed at three sites each and for wallflower mustard, catchweed bedstraw, field violet, Galeopsis spp., and Lamium spp. at one site each. At three sites, no significant treatment effects on crop yields were found, whereas yields at the remaining seven sites were higher where guidelines had been used than in other treatments in several years. It is concluded that application of dose rates according to recommendations from guidelines can be a fruitful way to reduce herbicide use.

A sulfonylurea (SU) herbicide resistance allele discovered in prickly lettuce was previously transferred to domestic lettuce with the cultivar name ‘ID-BR1’. ID-BR1 was acquired, and the SU resistance allele was transferred through traditional breeding methods to five common commercial lettuce types: butterhead, crisphead, green leaf, red leaf, and romaine. Field trials were conducted at Salinas, CA during 2011 and 2012 to evaluate POST applications of tribenuron-methyl (tribenuron) on SU-susceptible and SU-resistant lettuce types. Treatments included a nontreated control, pronamide applied PRE at 1,340 g ai ha−1, and tribenuron at 4, 9, and 17 g ai ha−1 applied POST. Data collected were: weed control, crop injury estimates (0 = safe, 100 = dead), stand counts, and lettuce yields. Injury to lettuce from tribenuron was high in SU-susceptible lettuce types and low in SU-resistant accessions. With the exceptions of a romaine lettuce line that still may have some susceptible individuals, tribenuron did not reduce yield of SU-resistant lettuce, but did reduce the yield of SU-susceptible lettuce. Suppression of weeds such as common groundsel and annual sowthistle was higher with tribenuron than with pronamide. Tribenuron should be considered for registration as a lettuce herbicide for SU-resistant lettuce to improve current weed management options for that crop.

Different herbicide-application strategies may serve as part of an integrated weed management (IWM) system. In 1987 and 1988, ten field trials were initiated in the south-central part of Sweden, with the objective of studying the long-term effects of herbicide-application strategies on the development of weed populations and productivity of spring-sown cereals. Each year until 1997, herbicides were applied at 25, 50, 75, or 100% of the full recommended dose. Treatments included herbicide exclusion every second year and herbicide application at 25 or 50% of a full dose during two out of three years, with a full dose applied in the third year. An untreated control was included. In 1998, weed densities in 25, 50, 75, and 100% of a full dose were reduced 43, 58, 64, and 67% compared with the control. At seven sites, the exclusion of herbicides every second year resulted in 43 to 178% higher weed densities than in 50% of a full dose, although the same amount of herbicide was applied when summarized over 2 yr. At four sites, herbicide application each year at 50, 75, or 100% of a full dose increased the density of difficult-to-control species by 24% compared with the untreated control. Averaged over sites, the untreated control contained 30% more weed species than herbicide application at 75 or 100% of a full dose. At three sites in 1996, the full dose resulted in 520 kg ha–1 (pooled over sites) higher barley yields than in the control. Neither in 1996 nor in 1997, was there any difference in crop yield between herbicide application at 25 and 100% of a full dose. This study demonstrates the potential of reducing the input of agrochemicals for weed control by using herbicides at reduced rates in competitive crops.

Field studies were conducted to evaluate POST herbicides with adjuvants for the control of three-cornered jack in winter wheat. The herbicides evaluated were fluroxypyr 9.7% w/w + MCPA 38.8% w/w (formulated mixture [450 g ai ha−1]), carfentrazone-ethyl (20 g ai ha−1), bromoxynil 26.8% w/w + MCPA 40.0% w/w (formulated mixture [450 g ai ha−1]), thifensulfuron-methyl (75 g ai ha−1), and tribenuron-methyl (75 g ai ha−1), applied alone or tank mixed with the adjuvants alkyl ether sulfate sodium salt (625 mL ha−1) or fatty alcohol ethoxylate (375 mL ha−1). The addition of adjuvants to carfentrazone-ethyl resulted in > 94% control of three-cornered jack. Both adjuvants enhanced the efficacy of all herbicides for control of three-cornered jack, except thifensulfuron-methyl. However, a maximum increase in phytotoxicity on three-cornered jack was achieved with the addition of either adjuvant to tribenuron-methyl compared with herbicides used alone. Bromoxynil + MCPA and carfentrazone-ethyl gave maximum wheat yield and yield components.

The affect of mesosulfuron and tribenuron applied POST to oat and rye was determined at three locations in Georgia during 2004 and 2005. Five herbicide treatments were applied to oat and rye in Feekes scale (FS) 1.2 or 2 developmental stage. Herbicide treatments consisted of tribenuron at 13 or 26 g ai/ha, tribenuron at 13 g/ha plus MCPA at 561 g ai/ha, MCPA at 561 g/ha, and mesosulfuron at 15 g ai/ha. Tribenuron, MCPA, and tribenuron plus MCPA injured both crops less than 4% and did not affect grain yield. Mesosulfuron applied to rye in FS 1.2 injured the crop 36% at 2 wk after treatment (WAT), 12% at 5 WAT, and 0% at harvest. Mesosulfuron applied at FS 2 injured rye less than 9%. Mesosulfuron did not influence rye-grain yield with either application timing. Mesosulfuron injured oat 65 to 86% at 5 WAT and reduced yield at least 70% regardless of application timing.

Research was conducted to establish a method to investigate the resistance level of flixweed to tribenuron-methyl and the evolved biochemical resistance mechanism. Four resistant biotypes were collected from wheat fields in Mazhuangcun, Jiacun, Dishangcun, and Bafangcun in the Hebei province of China where tribenuron-methyl had been continuously used for more than 10 yr. Two susceptible biotypes were collected from wheat fields where tribenuron-methyl was never applied. Different biotypes were assessed by petri-dish bioassay, whole-plant bioassay, and acetolactate synthase (ALS) assay. Comparisons of data indicated a similarity between methods and that experiments demonstrated that petri-dish bioassay was a feasible method to identify flixweed resistant to tribenuron-methyl. Data indicated differences among the flixweed biotypes when assessed by the petri-dish bioassay, whole-plant bioassay, or ALS enzyme assay, and a close association was obtained for the three bioassay methods. ALS resistance varied by biotypes with Mazhuangcun > Jiacun > Dishangcun > Bafangcun. Target-site enzyme assay data indicated that the resistant biotype's enhanced ALS activity was the biochemical mechanism that induced flixweed's evolved resistance to tribenuron-methyl. The concentrations of tribenuron-methyl causing 50% inhibition of ALS activity of the four resistant biotypes were 1,359, 513, 184, and 164 nM; in the susceptible biotypes these concentrations were 64 and 65 nM. Resistance indexes were 21, 8, 3, and 3 for Mazhuangcun, Jiacun, Dishangcun, and Bafangcun biotypes, respectively.

In a repeated multi-year study, mother potato plants were exposed to herbicides at rates that simulated off-target application, such as through tank contamination. Following exposure of mother plants to herbicides, seed from mother plants was planted in the following growing season and crop growth, yield and tuber quality were quantified. Visual injury from herbicides was observed both in the mother plant and daughter tuber growing seasons and occasional impacts on tuber yield were noted. However, an inconsistent relationship was observed for herbicide related injury and tuber yield reductions of mother potato plants with daughter tuber growth and yield. The lack of consistency in the relationship between visual potato injury in the mother plant production and adverse daughter tuber growth and yield in the following year challenges traditional crop scouting as a tool to predict off-target herbicide risk near seed potato production.