Using a high protein diet (483 g/kg), two trials were carried out to assess any potential benefit which may result from the supplementation of diets for fingerling and grower class gilthead seabream with pyridoxine at levels which were below, matched and far exceeded the minimum dietary requirement. At the level of supplementation below the minimal requirement (lowest level) the responses from the practical diet almost matched the responses from the diet containing an amount of pyridoxine corresponding to the published minimum requirement for the species and no significant improvement in performance was recorded in response to increasing supplement level. However, despite the absence of a marked effect on overall performance, a small potential for increased activity of alanine aminotransferase was recorded amongst grower class fish given diets containing the lowest supplement. The proximate composition of the grower class fish was unaffected by the level of pyridoxine supplementation though marginal increases in the lipid content of the fingerlings were observed. At the haematological level, haematocrit, total haemoglobin and the plasma concentrations of glucose and protein were also unaffected. However, on the application of an acute stressor (repeated netting), significant alterations in haematocrit and plasma glucose concentration reflecting dietary pyridoxine supplement were recorded.