Glyphosate, alone and in combination with other herbicides or additives, was evaluated as a spot or shielded treatment for the control of silverleaf nightshade in cotton in three experiments over four years. Glyphosate used at 10.8 g ae/L in 1985 on dryland cotton controlled 81 to 98% of the silverleaf nightshade, but cotton lint yields were reduced by all treatments compared to the weed-free check. Glyphosate used at 7.2 g ae/L in 1986 on irrigated cotton controlled 6 to 83% of the silverleaf nightshade, and lint yields were reduced only when multiple spot applications were made. Spot applications of glyphosate were effective for silverleaf nightshade control when applied 7 wk after crop emergence in 1988. Weed control and cotton injury were lower with shielded applications than spot applications. In 1989, retreatments during a season or the next year resulted in 95% or greater silverleaf nightshade control. Cotton yield increases from herbicide treatment were not observed in 1988; however, yield increases were observed in 1989 when spot treatments were applied to the more successful 1988 plots.