The war in Donbas led some observers to speculate that this event might threaten intergroup relations in Ukraine. While studies in the 1990s indicated relatively positive attitudes between the different ethnic and linguistic groups, it has not been analyzed systematically how these attitudes have developed over time. Such an analysis contributes to our general understanding as to how war and nation-building politics affect attitudes toward minorities. Analyzing survey data from the Kyiv International Institute of Sociology from 1995 to 2018 using multivariate statistical methods, I show that the prejudice toward Russian-speaking Ukrainians – measured using the social distance scale – has increased since 2014, when both the war and Poroshenko’s presidency began, although the rise is rather small. A likely explanation to this phenomenon is the perceived link between Russian speakers and Russia as the aggressor in the war. The fact that Yushchenko’s presidency (2006–2009) did not result in a similar increase of negative sentiments, despite similarities between Yushchenko’s and Poroshenko’s identity politics, allows me to suggest that the higher level of prejudice under Poroshenko cannot be solely explained by the political rhetoric promoting an ethnic Ukrainian identity. However, the interplay of political rhetoric and war might have been relevant.