The herbicides that inhibit very-long-chain fatty acid (VLCFA) elongases are primarily used for residual weed control in corn, barley, oat, sorghum, soybean, sugarcane, certain vegetable crops, and wheat production fields in the United States. They act primarily by inhibiting shoot development of susceptible species, preventing weed emergence and growth. The objectives of this review were to summarize 1) the chemical family of VLCFA-inhibiting herbicides and their use in the United States, 2) the VLCFA biosynthesis in plants and their site of action, 3) VLCFA-inhibitor resistant weeds and their mechanism of resistance, and 4) the future of VLCFA-inhibiting herbicides. After their reclassification as Group 15 herbicides to include shoot growth-inhibiting herbicides (Group 8), the VLCFA-inhibiting herbicides are currently represented by eight chemical families (benzofurans, thiocarbamates, α-chloroacetamides, α-oxyacetamides, azolyl-carboxamides, isoxazolines, α-thioacetamides, and oxiranes). On average, VLCFA-inhibiting herbicides are applied once a year to both corn and soybean crops in the United States with acetochlor and S-metolachlor being the most used VLCFA-inhibiting herbicides in corn and soybean production, respectively. The site of action of Group 15 herbicides results from inhibition of the VLCFA synthase, which is encoded by several fatty acid elongase (FAE1)-like genes in VLCFA elongase complex in an endoplasmic reticulum. The VLCFA synthase is a condensing enzyme, and relies on a conserved, reactive cysteinyl sulfur in its active site that performs a nucleophilic attack on either the natural substrate (fatty acyl-CoA) or the herbicide. As of August 2023, 13 weed species have been documented to be resistant to VLCFA inhibitors, including 11 monocot weeds and two dicot weeds (Palmer amaranth and waterhemp). The isoxazolines (pyroxasulfone and fenoxasulfone) are the most recently (2014) discovered VLCFA-inhibiting herbicides. Although the intensity of VLCFA-inhibitor-directed discovery efforts has decreased over the past decade, this biochemical pathway remains a viable mechanistic target for the discovery of herbicide premixes and a valuable component of them.

The herbicides that inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD) are primarily used for weed control in corn, barley, oat, rice, sorghum, sugarcane, and wheat production fields in the United States. The objectives of this review were to summarize 1) the history of HPPD-inhibitor herbicides and their use in the United States; 2) HPPD-inhibitor resistant weeds, their mechanism of resistance, and management; 3) interaction of HPPD-inhibitor herbicides with other herbicides; and 4) the future of HPPD-inhibitor-resistant crops. As of 2022, three broadleaf weeds (Palmer amaranth, waterhemp, and wild radish) have evolved resistance to the HPPD inhibitor. The predominance of metabolic resistance to HPPD inhibitor was found in aforementioned three weed species. Management of HPPD-inhibitor-resistant weeds can be accomplished using alternate herbicides such as glyphosate, glufosinate, 2,4-D, or dicamba; however, metabolic resistance poses a serious challenge, because the weeds may be cross-resistant to other herbicide sites of action, leading to limited herbicide options. An HPPD-inhibitor herbicide is commonly applied with a photosystem II (PS II) inhibitor to increase efficacy and weed control spectrum. The synergism with an HPPD inhibitor arises from depletion of plastoquinones, which allows increased binding of a PS II inhibitor to the D1 protein. New HPPD inhibitors from the azole carboxamides class are in development and expected to be available in the near future. HPPD-inhibitor-resistant crops have been developed through overexpression of a resistant bacterial HPPD enzyme in plants and the overexpression of transgenes for HPPD and a microbial gene that enhances the production of the HPPD substrate. Isoxaflutole-resistant soybean is commercially available, and it is expected that soybean resistant to other HPPD inhibitor herbicides such as mesotrione, stacked with resistance to other herbicides, will be available in the near future.

Cover crops can provide many benefits to peanut and cotton crops planted in rotation including suppressing weeds, conserving soil moisture after termination, increasing soil organic matter, and reducing soil erosion. However, herbicide carryover can affect cover crop establishment. The objective of this study was to investigate the responses of 6 cover crops (daikon radish, cereal rye, oat, crimson clover, winter wheat, and common vetch) to 12 soil residual herbicides. A multiyear (2016–2018), multilocation study was conducted in Macon and Henry counties, Alabama. Herbicide treatments included S-metolachlor, acetochlor, pyroxasulfone, diclosulam, imazapic, chlorimuron-ethyl, bentazon plus acifluorfen, pyrithiobac-sodium, trifloxysulfuron-sodium, diuron, prometryn, and flumioxazin, each applied at 10% of the full-labeled rate. At 42 to 52 and 145 to 149 d after planting (DAP), cover crop plant heights and stand counts were evaluated, as was biomass at 145 to 149 DAP. Treatments varied from year to year but not locations. In 2016, significant stand reductions (P ≤ 0.10) of 36% to 43% in rye and 44% to 75% in wheat were observed at 48 to 52 DAP for S-metolachlor, acetochlor, pyroxasulfone, imazapic, and bentazon plus acifluorfen compared with nontreated plants. Vetch had stand reductions ranging from 14% to 80% for all treatments 50 DAP except for plants treated with prometryn. S-metolachlor, pyroxasulfone, and acetochlor reduced stands of rye, wheat, and vetch more than any other herbicides. In 2017, at 147 to 149 DAP, clover stands were reduced by 29% with diclosulam and by 38% with trifloxysulfuron-sodium. Similarly, radish stands were reduced by 64% with diclosulam treatment. No significant biomass reductions were observed for any cover crop species either year. Oat showed the most tolerance with no treatments reducing any growth parameters either year. Although initial injury and stunting may occur, biomass at termination of cover crops were not affected by herbicide residues evaluated in this study.

Cover crops have increased in popularity in midwestern U.S. corn and soybean systems in recent years. However, little research has been conducted to evaluate how cover crops and residual herbicides are effectively integrated together for weed control in a soybean production system. Field studies were conducted in 2016 and 2017 to evaluate summer annual weed control and to determine the effect of cover crop biomass on residual herbicide reaching the soil. The herbicide treatments consisted of preplant (PP) applications of glyphosate plus 2,4-D with or without sulfentrazone plus chlorimuron at two different timings, 21 and 7 d prior to soybean planting (DPP). Cover crops evaluated included winter vetch, cereal rye, Italian ryegrass, oat, Austrian winter pea, winter wheat, and a winter vetch plus cereal rye mixture. Herbicide treatments were applied to tilled and nontilled soil without cover crop for comparison. The tillage treatment resulted in low weed biomass at all collection intervals after both application timings, which corresponded to tilled soil having the highest sulfentrazone concentration (171 ng g−1) compared with all cover crop treatments. When applied PP, herbicide treatments applied 21 DPP with sulfentrazone had greater weed (93%) and waterhemp (89%) control than when applied 7 DPP (60% and 69%, respectively). When applied POST, herbicide treatments with a residual herbicide resulted in greater weed and waterhemp control at 7 DPP (83% and 77%, respectively) than at 21 DPP (74% and 61%, respectively). Herbicide programs that included a residual herbicide had the highest soybean yields (≥3,403 kg ha−1). Results from this study indicate that residual herbicides can be effectively integrated either PP or POST in conjunction with cover crop termination applications, but termination timing and biomass accumulation will affect the amount of sulfentrazone reaching the soil.

Imazapyr and imazamox are frequently applied postemergence to control grass and broadleaf weeds in imidazolinone-resistant sunflower in Argentina. Herbicide carryover to rotational crops represents a disadvantage of these herbicides, particularly in regions with low rainfall during the months prior to rotational crop sowing. Between 2009 and 2012, field and greenhouse studies were conducted on four important sunflower-cropped areas of Argentina. The objective was to quantify the effects of imazapyr alone and imazamox plus imazapyr applied in sunflower crops on the subsequent establishment, growth, and yield of barley, oat, and wheat. In all field experiments, imazapyr alone and imazamox plus imazapyr were applied at recommended rates (80 gha–1 and 66 plus 30 gha–1, respectively), and also, in some experiments, at double the recommended rates. Soil bioassays were also conducted in the greenhouse to study the effect of these herbicides on barley, oat, and wheat seedlings. The mixture of imazamox plus imazapyr was safer for rotational crops than imazapyr applied alone, because of the reduced rate of imazapyr in the mixture treatments. Barley was more sensitive to imidazolinones, particularly imazapyr, than the other winter cereals. Imazapyr at double rate (160 gha–1) reduced barley yield by 45% when seeds were sown 165 d after herbicide application and with 240 mm rainfall after herbicide application.

Weed management is a major constraint in organic cropping systems. In 2004, the Cornell Organic Vegetable Cropping Systems Experiment was established in central New York state using a split-plot randomized complete block design with two crop rotation entry points (split-plot factor). Four organic vegetable cropping systems that varied in cropping intensity and tillage (main plot factor) were compared: (1) intensive, (2) intermediate, (3) bio-extensive, and (4) ridge tillage. The basic crop rotation was cabbage, lettuce, potato, and winter squash, with additional short-season crops in the intensive system and with cover crops and fallow substituted for cabbage and potato in the bio-extensive system. In 2014, two uniformity trials were conducted in which oat and then a mixture of sorghum-sudangrass plus Japanese millet were grown uniformly over the entire experiment. Prior to sowing oat, soil samples were collected from each plot and an emergence bioassay was conducted to assess the soil weed seedbank. Crop biomass, weed density, and weed biomass were sampled in the uniformity crops. Soil weed seedbank density was three to four times greater in the intensive, intermediate, and ridge-tillage systems than in the bio-extensive system. The bio-extensive system also had lower weed density and weed biomass in the oat uniformity trial compared with the other three systems. Oat biomass did not differ between the cropping systems. Weed density and biomass in oat were also affected by the crop rotation entry point. Cropping system legacy effects on weed abundance and community composition were greater in the oat than in the sorghum-sudangrass plus Japanese millet uniformity trial. Our results illustrate the effects of different organic vegetable production practices on weed community structure and highlight the value of tilled fallow periods, cover crops, and prevention of weed seed rain for reducing weed populations.

A field study was conducted for the 2014 and 2015 growing season in Arkansas, Indiana, Illinois, Missouri, Ohio, and Tennessee to determine the effect of cereal rye and either oats, radish, or annual ryegrass on the control of Amaranthus spp. when integrated with comprehensive herbicide programs in glyphosate-resistant and glufosinate-resistant soybean. Amaranthus species included redroot pigweed, waterhemp, and Palmer amaranth. The two herbicide programs included were: a PRE residual herbicide followed by POST application of foliar and residual herbicide (PRE/POST); or PRE residual herbicide followed by POST application of foliar and residual herbicide, followed by another POST application of residual herbicide (PRE/POST/POST). Control was not affected by type of soybean resistance trait. At the end of the season, herbicides controlled 100 and 96% of the redroot pigweed and Palmer amaranth, respectively, versus 49 and 29% in the absence of herbicides, averaged over sites and other factors. The PRE/POST and PRE/POST/POST herbicide treatments controlled 83 and 90% of waterhemp at the end of the season, respectively, versus 14% without herbicide. Cover crop treatments affected control of waterhemp and Palmer amaranth and soybean yield, only in the absence of herbicides. The rye cover crop consistently reduced Amaranthus spp. density in the absence of herbicides compared to no cover treatment.

Velvetleaf is difficult to control in corn and soybean and the seed can persist in soil for many years. Seven cultural and tillage practices were established in 1974 on a site heavily infested with velvetleaf to determine the time required to eradicate velvetleaf seed from the soil. A rapid decline in velvetleaf seed population in the top 23 cm of soil occurred during the first 5 yr of this study. In the fifth year, the chemical fallow and continuous alfalfa treatments had 37 and 56% of the original velvetleaf seed population remaining, respectively. In the 17th year, soils in these treatments that had received no tillage since study initiation still contained 15 and 25% of the original velvetleaf seed population, respectively. Systems involving moldboard plowing with continuous-tillage fallow, continuous cropping of corn or oat, or an annual corn and soybean rotation had a more rapid decline in the velvetleaf seed population in soil compared to the chemical fallow and continuous alfalfa treatment. After 17 yr, soil in any system that had received at least one moldboard plowing per year still contained 1 to 3 million velvetleaf seed ha−1, which is only 0.8 to 2.5% of the initial viable seed population. Nearly 100% of the seed remaining in the soil in the 17th year for all treatments was still viable.

One year after application under irrigated conditions, atrazine at 1.2 kg/ha injured oat but there was no effect after two years. On dryland, wheat and barley were injured for up to two years after atrazine was applied at 1.5 kg/ha. Atrazine concentrations in the soil were related to accumulated rainfall in an exponential equation. The equation predicted that, under the most severe drought conditions ever recorded for southern Alberta, injury to cereal crops could occur for at least three years after atrazine application at 1.2 to 1.5 kg/ha. Cyanazine did not injure subsequent crops under either irrigated or dryland conditions.

The response of oat, foxtail millet, proso millet, and sunflower to atrazine and clomazone applied the previous fall was investigated two years in field studies near Akron, CO and Sidney, NE. Foxtail millet biomass, and proso millet and sunflower grain yields were not reduced when these crops were seeded into soil that had been treated the previous fall with atrazine and/or clomazone at rates of 0.6 or 1.1 kg ai/ha. Forage yield of oat was reduced 11 to 18% by some treatments, but this effect was not consistent over years or sites. Treatments containing 1.1 kg/ha of atrazine provided 1 to 5 wk of residual weed control in foxtail millet, proso millet, and sunflower. The study indicated that producers have flexibility in crop selection when using atrazine and clomazone in reduced- and no-till production systems.

A practical expert system is needed to handle POST weed control in herbicide-intensive crops such as wheat, barley, oat, rye, sugarbeet, corn, and sorghum. SELOMA is an expert system having a step-by-step problem-solving procedure closely resembling what a weed management expert would follow. It is based on field surveys of weed density, and crop and weed growth stage and height. SELOMA evaluates weed competitiveness and provides weed management advice. It suggests whether or not to intervene, chemical and mechanical weed control treatments, and selects the best herbicides, including commercial formulations, costs, and optimal dosages.

Certain salts in the spray carrier can antagonize sethoxydim, while other salts overcome the antagonism or increase the herbicide's efficacy. Sethoxydim responses to salts have not been consistent, and the inconsistency is not completely understood. Experiments were conducted in the greenhouse to determine sethoxydim phytotoxicity to oat as influenced by salts in the spray carrier and by air temperature at and immediately after application as they alter the spray deposit characteristics. Thick amorphous spray deposits were related to ineffective sethoxydim-petroleum oil adjuvant treatments applied alone at 10 C or with bentazon at 10 or 25 C. Also, thin deposits with salt residues were related to ineffective sethoxydim treatments, as when sodium bicarbonate was present. Ammonium sulfate reduced or overcame antagonism of sethoxydim phytotoxicity caused by low temperature, bentazon, and sodium bicarbonate and gave thin deposits having close contact with the leaf epicuticular surface but without obvious salt deposits. UV light antagonism of sethoxydim phytotoxicity was different depending on temperature at and shortly after application and depending on salts in the spray mixture. The results indicate that salts in a bentazon spray carrier are in part antagonistic because of their effect on final spray deposit.

Field experiments were conducted from 1985 through 1989 to evaluate herbicide selectivity and impact on seeding-year yields of spring oat and underseeded alfalfa, and carryover weed control benefits from increased legume-fixed N for second-year dryland no-till corn. PRE metolachlor, pendimethalin, and prodiamine controlled green foxtail and POST bromoxynil or 2,4-DB controlled broadleaf weeds. These herbicides caused 0 to 20% alfalfa injury and 0 to 17% oat injury, and increased oat yield one of three years but did not increase the yield of underseeded alfalfa. POST pyridate, thifensulfuron, and tribenuron were too injurious to either oat, alfalfa, or both crops. Forage yields of annual ‘Nitro’ and perennial ‘Wrangler’ alfalfa seeded alone were greater than when they were underseeded in oat, with herbicides applied in both systems. As a result of drought in 1988 and 1989, yield of second-year corn planted after one-year alfalfa was not increased from potentially greater legume-fixed N. Dryland corn yield following monoculture oat or corn was 254% higher than corn following alfalfa.

Controlled-release formulations have the potential to reduce atrazine leaching but may reduce herbicide bioavailability and weed control. This research was conducted to determine if starch-encapsulated atrazine produced by the extrusion process could be modified to provide an optimum rate of herbicide release for adequate weed control and reduced leaching potential in sandy soils. The dry flowable (DF) and six starch-encapsulated atrazine formulations of various mesh size (14 to 20 and 20 to 40), clay content (0 and 5%), and extruder processing speed (200 and 400 rpm) were evaluated. All starch encapsulations reduced the amount of atrazine leaching from the surface 5 cm of soil columns by an average of 68% compared with the dry flowable formulation. Compared with the larger starch granules (14 to 20 mesh) 8% more atrazine in the smaller granules leached from the surface 5 cm. Atrazine efficacy was measured using an oat bioassay conducted in the greenhouse. Smaller granules (20 to 40 mesh) provided equivalent efficacy to DF while large granules (14 to 20 mesh) did not. Extruder speed and clay content did not affect efficacy or leaching potential. This study indicates that finer starch granules should result in faster atrazine release and improved weed control while still reducing the leaching potential of atrazine.

Studies were conducted at the Vegetable Substation in Kibler, AR, in 1992 and 1993, in the same plots, to evaluate weed suppression by spring-seeded cover crops and to determine the effects of cover crop and imazethapyr on no-till southern pea. A plot without cover, conventionally tilled before planting southern pea, served as control. Weed control treatments, applied as subplots in each cover crop, included a weedy check, handweeded check, and half and full rates of imazethapyr (0.035 and 0.07 kg/ha) followed by sethoxydim (0.22 kg/ha). Biomass of Palmer amaranth 6 WAR without herbicides, was less in Italian ryegrass and sorghum-sudangrass residues than in oat residue and no cover crop. Over the years, Palmer amaranth density increased 333% without cover crops and 28% with cover crops. Rice flatsedge density increased four to five times in oat and sorghum-sudangrass residues but remained the same in Italian ryegrass residue. In general, Italian ryegrass residue suppressed the most weeds. Oat residue was least suppressive. Italian ryegrass and sorghum-sudangrass also reduced southern pea stand. Regardless of cover crop and year, half and full rates of imazethapyr followed by sethoxydim equally reduced density of Palmer amaranth, goosegrass, large crabgrass, southwestern cupgrass, and rice flatsedge compared with the untreated check. Residual control of Palmer amaranth by imazethapyr was higher at the full rate than the reduced rate, regardless of cover crop. Half rate of imazethapyr followed by sethoxydim controlled 94 to 100% of Palmer amaranth, rice flatsedge, large crabgrass, and southwestern cupgrass late in the season, regardless of cover crop in 1992 and 1993. Southern pea yield in untilled plots with cover crops was two to three times lower than yield in plots with preplant tillage and no cover crops mostly because of reduction in crop stand in the presence of cover crops.

Field studies were conducted over 3 yr in central Spain to investigate the tolerance of common vetch and oat to some preemergence herbicides and their effects on weed control, forage yields, and botanical composition of the forage mixture. Pendimethalin was the only herbicide that injured common vetch. Pronamide and pronamide plus diuron injured oat by affecting plant emergence. Prevalent weed species were fumitory, henbit, and wild buckwheat. All herbicides provided more than 90% control of fumitory and most herbicides except pronamide provided more than 90% control of henbit relative to the untreated check. Wild buckwheat stands were reduced by isoxaben (68%), linuron (40%), prometryn (69%), pronamide (86%), and pronamide plus diuron (61%). More than 90% control of prostrate knotweed was achieved with isoxaben, pronamide, and terbutryn. Pronamide and pronamide plus diuron reduced forage yields and increased vetch in the forage. The untreated vetch and oat monocrop treatments showed the competitive advantage of oat over vetch and weedy species.

Experiments were conducted using oat in the greenhouse to determine phytotoxicity and spray deposit characteristics of sethoxydim on oat as influenced by adjuvants, ultraviolet (UV) light, and temperature at and shortly after application. Petroleum oil of low viscosity was equal or more effective with sethoxydim when applied at 10 than at 25 C, but high-viscosity petroleum oil was more effective at 25 than at 10 C. UV light reduced sethoxydim efficacy more for high- than low-viscosity petroleum oil adjuvant at 25 C, but not at 10 C. Commercial vegetable oil adjuvant was less effective with sethoxydim at 10 than at 25 C, and UV light reduced efficacy at both temperatures. Commercial methylated vegetable oil was the most effective adjuvant and efficacy was only slightly reduced by UV light at 25 C. A secondary alcohol surfactant that is considered hydrophilic enhanced sethoxydim efficacy even when exposed to UV light. Adjuvants that gave discernible uniform spray deposits with close contact to the epicuticular surface, including over anticlinal walls, resulted in the most effective treatments. The results indicated that temperature at application is important to adjuvant efficacy, partly because of the effect on spray deposit characteristics.

Experiments were conducted in the greenhouse to determine the effects of adjuvant and herbicide concentrations on imazamox, imazethapyr, nicosulfuron, and ICIA 0604 phytotoxicity, independent of spray retention. Equal amounts of herbicide were applied to oat in a single 0.5-μl drop or four 0.5-μl drops. Changes in adjuvant concentration influenced herbicide phytotoxicity more with application in one concentrated drop rather than in four dilute drops. Overall, herbicide phytotoxicity was greater when low adjuvant concentrations were applied in four dilute drops compared with a single concentrated drop. But when the same total amount of adjuvant was applied in one or four drops, herbicide phytotoxicity in a single drop was generally equal to or greater than in four drops. These results suggest that high herbicide phytotoxicity with high herbicide and adjuvant concentrations in low spray volumes in the field was primarily because of increased herbicide absorption rather than spray retention.

Dormancy is a critical factor for the survival and persistence of weedy species. Contemporary approaches can be used to identify genes that regulate dormancy directly or indirectly to elucidate key mechanisms, signals, and pathways. Several domesticated plant species have been used as model systems to mark quantitative trait loci (QTL) that affect dormancy in seeds and vegetative propagules directly. A few weedy species have also been used to mark QTL and to determine dormancy genes using microarray analysis. Given the number of serious weeds worldwide and the role that dormancy plays in their persistence, developing fundamental knowledge on dormancy is an important step toward developing new strategies for weed management. This paper describes current research and outlines some weeds that might be candidates for dormancy investigations using molecular genetic and genomics approaches. An underlying theme in the selection of weeds for dormancy investigations is their relation to crop species and the ability to adapt existing resources to investigate dormancy in weedy plants.

Potato, dry bean, and sugar beet production have increased markedly in recent years on irrigated cropland in Alberta, Canada. Concerns exist about declining soil quality and increased soil erosion when these low-residue crops are grown in sequence in short-duration rotations. A 12-yr rotation study was conducted to determine the merits of adopting various conservation practices (reduced tillage, cover crops, composted manure) and longer-duration rotations to develop a more sustainable production system for these row crops. This article reports on weed density and weed seedbank data collected in the study. Weed densities recorded prior to applying postemergence herbicides indicated that conservation compared with conventional management treatments had greater weed densities in 30 to 45% of the cases in 3-, 4-, and 5-yr rotations. In contrast, a 6-yr conservation rotation that included 2 yr of timothy forage resulted in similar or lower weed densities than rotations with conventional management practices. Residual weed densities recorded 4 wk after applying postemergence herbicides were only greater in conservation than conventional rotations in 2 of 12 yr, regardless of rotation length. Weed seedbank densities at the conclusion of the 12-yr study were similar for 3- to 6-yr rotations under either conservation or conventional management. These findings indicate that implementing a suite of conservation practices poses little risk of increased weed populations in the long term. This knowledge will facilitate grower adoption of more sustainable agronomic practices for irrigated row crops in this region.