This article examines the development and transformation of the holy spirit within Jewish mysticism. It begins with a brief analysis of primary trends concerning the holy spirit in biblical, rabbinic, and medieval Jewish texts that served as crucial material for the holy spirit’s ascendence in sixteenth-century Jewish mysticism. Following, it examines the writings of leading Jewish mystics: Moses Cordovero, Elijah de Vidas, and Ḥayyim Vital, who resided in the remote Galilean town of Safed. These luminaries each developed the concept of the holy spirit along a spectrum of pneumatic, fusionary, mystical, and revelatory experiences. Ultimately, they transformed the holy spirit into the peak experience of Jewish mystical life— experienced as prophecy, sanctification, and embodiment. This article highlights an important, yet understudied, Jewish mystical phenomenon.