Downy brome, feral rye, and jointed goatgrass are problematic winter annual grasses in central Great Plains winter wheat production. Integrated control strategies are needed to manage winter annual grasses and reduce selection pressure exerted on these weed populations by the limited herbicide options currently available. Harvest weed-seed control (HWSC) methods aim to remove or destroy weed seeds, thereby reducing seed-bank enrichment at crop harvest. An added advantage is the potential to reduce herbicide-resistant weed seeds that are more likely to be present at harvest, thereby providing a nonchemical resistance-management strategy. Our objective was to assess the potential for HWSC of winter annual grass weeds in winter wheat by measuring seed retention at harvest and destruction percentage in an impact mill. During 2015 and 2016, 40 wheat fields in eastern Colorado were sampled. Seed retention was quantified and compared per weed species by counting seed retained above the harvested fraction of the wheat upper canopy (15 cm and above), seed retained below 15 cm, and shattered seed on the soil surface at wheat harvest. A stand-mounted impact mill device was used to determine the percent seed destruction of grass weed species in processed wheat chaff. Averaged across both years, seed retention (±SE) was 75% ± 2.9%, 90% ± 1.7%, and 76% ± 4.3% for downy brome, feral rye, and jointed goatgrass, respectively. Seed retention was most variable for downy brome, because 59% of the samples had at least 75% seed retention, whereas the proportions for feral rye and jointed goatgrass samples with at least 75% seed retention were 93% and 70%, respectively. Weed seed destruction percentages were at least 98% for all three species. These results suggest HWSC could be implemented as an integrated strategy for winter annual grass management in central Great Plains winter wheat cropping systems.

Field experiments were conducted at five locations in Colorado, Kansas, and Wyoming in 1994–1995 and 1995–1996 to compare the effects of MON 37500 rate and application timings on downy brome control and winter wheat tolerance. MON 37500 at 18 to 35 g ha−1 applied preemergence or fall postemergence reduced downy brome density 71 to 92% in 1995. Spring-applied MON 37500 suppressed downy brome growth but did not reduce plant density. No application reduced downy brome density in 1996. At each location, downy brome was controlled best by MON 37500 applied preemergence or fall postemergence at 35 g ha−1. MON 37500 did not affect wheat height at Archer or Torrington, WY, and Burlington or Stratton, CO, but wheat treated preemergence or fall postemergence was taller than untreated wheat at Hays, KS, in 1995. Spring-postemergence-treated wheat at Hays in 1995 was shorter than untreated, preemergence-, or fall-postemergence-treated wheat. Wheat head density did not differ among treated and untreated plots at Torrington, but herbicide treatment increased wheat yields. Wheat head density increased with all MON 37500 treatments at Hays in 1995, as did yield. However, preemergence and fall-postemergence applications resulted in the highest wheat yields. No herbicide treatment affected head density or yield at Hays in 1996.

Greenhouse studies were conducted to determine the following: if treating henbit or downy brome with glyphosate increased populations of Fusarium solani f. sp. pisi and Pythium ultimum in soil and rhizosphere soil; if treating henbit or downy brome with glyphosate increased root colonization and infection by F. solani f. sp. pisi or P. ultimum; and, if henbit and downy brome are hosts of F. sohni f. sp. pisi or P. ultimum. Pythium ultimum populations increased only in soil containing glyphosate-treated henbit. Fusarium solani f. sp. pisi and P. ultimum populations increased in rhizosphere soil from glyphosate-treated henbit, while only P. ultimum populations increased in rhizosphere soil from glyphosate-treated downy brome. These results suggest that peas planted in soil where either downy brome or henbit had been treated with glyphosate could be exposed to higher populations of F. solani f. sp. pisi and P. ultimum. Root colonization and infection, plant height, and root weight data indicated that henbit and downy brome are alternate hosts of P. ultimum. F. sohni f. sp. pisi colonized, but did not readily infect roots of downy brome and henbit.

Research was conducted to determine the suitability of the Fermi-Dirac distribution function for modeling the seedling emergence of downy brome, johnsongrass, and round-leaved mallow, as influenced by burial depth. Six sets of previously published emergence data were used to formulate the model and test its adequacy. Two independent johnsongrass emergence data sets were used to validate the model. Constant temperature growth chamber studies were conducted to evaluate the effects of temperature and moisture on the model parameters. The Fermi-Dirac distribution function was found to adequately describe the seedling emergence of downy brome, johnsongrass, and round-leaved mallow as indicated by a good visual data fit, narrow confidence intervals for the model parameters, and regression analysis of observed vs. modeled data. Although this function is a model used in physical science, its parameters can be related to abiotic factors such as soil texture, temperature, and moisture.

Downy brome is one of the most troublesome winter annual weeds in winter wheat-fallow rotations in the central Great Plains. A 3-yr seed burial study was initiated to determine how long downy brome seed remained germinable when placed on the soil surface or 2.5 cm deep at four different times in four environments. Only 1 to 7% of the downy brome seed survived after 1 yr on the soil surface in chemical fallow and stubble mulch when deposited in August, but survival varied in September, October, and November. In 1970, a year with low fall and winter precipitation, 36 to 46% of the seed placed on the soil surface of chemical fallow in September, October, and November survived, compared with 1 to 8% for stubble mulch tillage. Early spring tillage covered more seed with soil, and downy brome seed survival decreased. When fall and winter precipitation was normal, stubble mulch and chemical fallow had 1 to 20% germinable seed remaining. Induced dormancy existed in some years. More downy brome seed survived when placed on the soil surface of crested wheatgrass sod (14 to 50%) than on smooth brome sod (0 to 36%). No differences existed among environments when downy brome seed was buried 2.5 cm deep. Only 0.4% of downy brome seed buried 2.5 cm survived after 1 yr when averaged across all environments.

Downy brome has converted millions of hectares of Great Basin rangelands from shrubland to annual grass-dominated systems. Methods for removing downy brome from sites that already have perennial grasses established are especially needed because of the difficulty in re-establishing perennial species. In this study, early spring applications of glyphosate alone and glyphosate plus imazapic were monitored for 2 yr. Cover of downy brome was reduced from 45 to 10% by spring application of glyphosate alone and to approximately 1% with the addition of imazapic. Perennial grass cover was not affected by the spring application of glyphosate. The addition of imazapic reduced perennial grass cover in the first year; however, it recovered by the second year. The selective response to glyphosate may be due to differences in growth stage or dormancy characteristics between annual and perennial species. Our findings suggest spring application of glyphosate may provide an alternative approach for managing annual grasses on Great Basin rangelands.

Annual bromes (downy brome and Japanese brome) have been shown to decrease perennial grass forage production and alter ecosystem functions in northern Great Plains rangelands. Large-scale chemical control might be a method for increasing rangeland forage production. Although fall application has been shown to be the most effective and least likely to impact co-occurring native species, spring germination of downy brome may reduce the efficacy of fall-only herbicide application. We assessed the impact of a low glyphosate dose rate (210 g ha−1) applied to rangelands in fall or in fall and spring on nontarget species and on annual brome abundance at two sites in eastern Montana over 2 yr. We tested the following hypotheses: (1) nontarget effects are greater with spring herbicide application, (2) fall and spring herbicide application are necessary for effective downy brome control, and (3) fall herbicide application is sufficient to control Japanese brome. Few nontarget effects occurred; two dicotyledonous species exhibited small increases in response to herbicide. We found that that a single fall application reduced downy brome cover and seed bank density, but after the second fall application in the following year, downy brome did not continue to show a response to herbicide. After 2 yr of fall herbicide application, Japanese brome had denser seed banks in plots where herbicide had been applied. Blanket glyphosate application on rangelands is an unreliable method for controlling annual brome invasions in the northern Great Plains.

Recent changes in herbicide registrations and governmental restrictions on field burning raised many management questions for Kentucky bluegrass seed producers, particularly the extent to which useful lives of their stands might be shortened by decreasing crop yields or increasing weed pressure. Tests conducted over the lives of two grass seed stands (1993–1997) evaluated three contrasting methods of postharvest residue management (vacuum sweep, bale/flail chop/rake, and field burn) and 13 herbicide treatments. Downy brome was the primary weed at both the Madras and LaGrande, OR, sites. In nontreated checks and the four least effective herbicide treatments, downy brome populations increased exponentially over time, with year-to-year increases in density averaging 13.1-fold. Competition had easily detected effects on Kentucky bluegrass seed yield at densities of 30 downy brome plants/m2, and crop stands were destroyed beyond 100 to 200 weeds/m2. Both PRE terbacil at 840 g/ha and early POST (EPOST)/late POST (LPOST) split-applied primisulfuron at 20 g/ha per application contained downy brome during the first 2 yr but not the third, when crop injury from terbacil forced reduction in terbacil rate and changes in weed populations overcame primisulfuron. PRE terbacil followed by LPOST primisulfuron, EPOST terbacil plus primisulfuron followed by LPOST primisulfuron, and EPOST/LPOST split-applied terbacil plus primisulfuron achieved excellent control of downy brome until the final years of the study, when control became increasingly erratic as primisulfuron-resistant downy brome proliferated in specific individual plots. Injury from combination terbacil plus primisulfuron treatments reduced yield relative to safest treatments in early years when downy brome population densities were low.

Because of disturbance and exotic plant invasions, ecological restoration is necessary for maintaining functional big sagebrush ecosystems in western North America. Downy brome control is often necessary in restoring this ecosystem type; however, many brome control measures hinder ecological restoration by limiting the types of plants which can be established. Microtopography manipulation may aid weed control by entrapping undesirable seeds. We undertook a field experiment at four sites in the Piceance Basin of western Colorado, USA to test the effects of microtopography (rough with brush mulch or flat with straw mulch), seed mix (high-forb or balanced), and herbicide (140 g ai ha−1 imazapic ammonium salt or none) on downy brome control and perennial plant establishment following disturbance. Three years post-treatment, downy brome had become established at two of the four sites, one each with high (GVM) and low (MTN) downy brome seed rain. At GVM, the rough/brush treatment augmented the effectiveness of imazapic, reducing downy brome biomass six-fold. At MTN, the rough/brush surface reduced downy brome biomass 10-fold in the absence of imazapic. Across all four sites, forb and shrub biomass were higher with the high-forb mix, and there was no effect of seed mix on downy brome or annual forb biomass. Restoring a full complement of plant functional groups in big sagebrush ecosystems may be aided by increasing forbs in seed mixes, and manipulating soil microtopography.

This research explored the use of downy brome (BROTE) as a cover crop in irrigated corn. Although BROTE is a difficult weed to control, it could not be maintained as a cover crop in no-till irrigated corn for more than one season. A 10-fold reduction in BROTE occurred in the second year of corn. By the fourth year, only one BROTE plant could be found at the two locations. Because BROTE did not persist across years, soil coverage decreased 5 to 18% in the later location-years. At one location, normal herbicide rates decreased Johnsongrass biomass more than 22-fold both years it was applied. Increasing herbicide input decreased Palmer amaranth density more than 3-fold, but only in a single location-year. In three of six location-years, level of herbicide input had no significant effect on evapotranspiration (ET). Increased BROTE biomass decreased ET 0.033 to 0.083 cm/d during the first season at both locations. Increased irrigation increased corn yield by 240 to 1,900 kg/ha in five of six location-year combinations. Half rates of in-season herbicides reduced yield only in one of six location-years. High BROTE density reduced ET but did not translate into increased crop yield. In three of six location-year combinations, high BROTE density decreased yield by 300 to 1,000 kg/ha. In a single location-year, increased surface residues provided by BROTE increased yield by 560 kg/ha. Increased irrigation inputs decreased water use efficiency (WUE) by 6.3 kg/ha-cm in a single location-year and increased WUE by 10.8 to 121.6 kg/ha-cm in four of six location-years. Increased herbicide inputs increased WUE by 10.3 kg/ha-cm in one location-year. BROTE density had no significant effect on WUE at location 1. At location 2 in the first 2 yr, WUE was increased 9.4 to 22.2 kg/ha-cm.

Winter annual weeds are becoming prolific in agricultural fields in the midwestern United States. The objectives of this research were to understand the roles of soil temperature (daily average and fluctuation) and moisture on the emergence of nine winter annual weed species and dandelion and to develop predictive models for weed emergence based on the accumulation of modified thermal/hydrothermal time (mHTT). Experiments were established at Lincoln, NE; Mead, NE; and at two sites (irrigated and rainfed) near Clay Center, NE, in 2010 and 2011. In July of each year, 1,000 seeds of each species were planted in 15 by 20 by 6-cm mesh baskets installed between soybean rows. Soil temperature and water content were recorded at the 2-cm depth. Emerged seedlings were counted and removed from the baskets on a weekly basis until no additional emergence was observed in the fall, resumed in late winter, and continued until emergence ceased in late spring. Weather data were used to accumulate mHTT beginning on August 1. A Weibull function was selected to fit cumulative emergence (%) on cumulative mHTT (seven base temperature [Tbase ] by six base water potential [Ψbase ] by three base temperature fluctuation [Fbase ] candidate threshold values = 126 models); it was also fit to days after August 1 (DAA1), for a total of 127 candidate models per species. The search for optimal base thresholds was based on the theoretic-model comparison approach (Akaike information criterion [AIC]). All three components (Tbase , Ψbase , and Fbase ) were only important for Virginia pepperweed. For downy brome and purslane speedwell, including Tbase and Ψbase resulted in the best fit, whereas for dandelion including Tbase and Fbase resulted in the best fit. A model including only Tbase resulted in the best fit for most species included in this study (Carolina foxtail, shepherd's-purse, pinnate tansymustard, henbit, and field pansy). For field pennycress, the model based on DAA1 resulted in the best fit. Threshold values were species specific. Soil temperature was the major environmental factor influencing winter annual weed emergence. Even though soil moisture and often temperature fluctuation are essential for seed germination, Ψbase and Fbase were not as critical in the predictive models as initially expected. Most seedlings (> 90%) of downy brome, pinnate tansymustard, Carolina foxtail, henbit, and field pansy emerged during the fall. Virginia pepperweed, purslane speedwell, dandelion, shepherd's-purse, and field pennycress seedlings emerged during both fall and spring. The results of this research provide robust information on the prediction of the time of winter annual weed emergence, which can help growers make better management decisions.

Improved fallow systems are needed to reduce the negative effects of increased soil erosion and reduced soil quality. Field experiments were conducted to determine weed suppression attained with yellow sweetclover grown as a green manure fallow replacement crop. Yellow sweetclover was undersown in field pea, flax, or Indian mustard and then killed in June of the following fallow year. Living yellow sweetclover competed strongly with weeds during the first fall and spring of fallow. Weed biomass accounted for <1 to 12% of the total plant biomass when yellow sweetclover was terminated in June. Yellow sweetclover residues remaining after termination of growth continued to provide excellent weed suppression. Weed densities in April before planting the succeeding wheat crop were 75 to 97% lower in yellow sweetclover than in untreated fallow treatments. Yellow sweetclover controlled the perennial weeds dandelion and perennial sowthistle, as well as the annuals kochia, flixweed, Russian thistle, and downy brome. Weed suppression was similar whether yellow sweetclover was harvested as hay or its residues were incorporated or left on the soil surface, suggesting that a portion of the weed suppression effect may be due to allelopathic compounds being released from decomposing yellow sweetclover. Results will be used to develop more sustainable agronomic practices in regions where fallow is still widely employed.

In spring 2005, a downy brome population with possible resistance to fluazifop-P, an acetyl-CoA carboxylase (ACCase) inhibitor (group 1) herbicide was found in a commercial creeping red fescue seed production field, near La Grande, OR, where fluazifop-P had been used to control downy brome repeatedly over 7 yr. Greenhouse experiments were conducted to confirm resistance to a number of group 1 herbicides. The suspected resistant downy brome accession was tested for resistance to fluazifop-P and tested for cross-resistance to other aryloxyphenoxy propionate (APP) and cyclohexanedione (CHD) herbicides, including quizalofop-P, sethoxydim, and clethodim. Data recorded included plant-mortality counts and biomass. Tests revealed that the La Grande downy brome accession was highly resistant to fluazifop-P and sethoxydim at all tested rates. The La Grande accession suffered no mortality from fluazifop-P or sethoxydim treatments up to the maximum tested rate of eight times (8×) the labeled recommendation. The La Grande accession was resistant to quizalofop-P and clethodim at the labeled rate or less but was susceptible to application rates higher than the labeled rate. The control downy brome accession was completely susceptible to fluazifop-P, quizalofop-P, and clethodim at all rates and exhibited increasing susceptibility with increasing sethoxydim rate. This pattern of cross-resistance differs from that of a previously reported case of ACCase resistance in downy brome.

The invasive grass Bromus tectorum (cheatgrass, downy brome) has extensive ecological breadth in its introduced range, and is increasing in abundance at high elevations. As a predominantly selfing species with high potential population growth rates, it is a likely candidate for having general-purpose genotypes that facilitate its invasion into high elevations. We evaluated evidence for general-purpose genotypes by examining phenotypic and molecular variation in B. tectorum from low- and high-elevation sites in the Rocky Mountains. Common garden experiments revealed that plants from high elevations germinated faster, but no other phenological, morphological, or physiological differences were found between elevations. Leaf length was longer for low-elevation sites in a common garden using field-collected seeds, but this was not found in a common garden using greenhouse-grown seeds, suggesting maternal effects. For most traits, there was significant phenotypic variation among sites and individuals. Using microsatellites, we genotyped individuals from low- and high-elevation sites. We found no indication of genetic differentiation at these loci between high and low elevations, but there was significant variation within and among sites. No single genotype was common across sites. The success of B. tectorum is not due to a few general-purpose genotypes, but rather many genotypes associated with many different phenotypes. Understanding the mechanisms behind the success of invaders enhances our knowledge of basic evolutionary and ecological processes and is crucial to our ability to manage ongoing, and prevent future, invasions, especially into new habitats.

Enhanced herbicide metabolism is less common than target site–based herbicide resistance in weeds and often confers resistance to chemically dissimilar herbicides. In a previous study, the mechanism of acetolactate synthase (ALS)-inhibitor resistance in a downy brome biotype was determined to be metabolism. Our research was aimed at determining the multiple resistance pattern in the downy brome biotype, establishing its physiological basis, and investigating its fitness. Dose–response experiments showed that the resistant biotype was also moderately resistant to ethofumesate, clethodim, fluazifop, diuron, and terbacil and highly resistant to the triazine herbicides, atrazine and metribuzin. DNA sequence analysis of the psbA gene, which is the target site of PSII inhibitors, demonstrated a single amino acid substitution from serine to glycine in the resistant biotype at residue 264 in the D1 protein. Thus, the resistant biotype contains two different resistance mechanisms, herbicide metabolism and an altered target site. The resistant biotype produced less shoot dry weight, leaf area, and seed and was shorter than the susceptible biotype. The resistant biotype was also less competitive than the susceptible biotype. Thus, in the absence of herbicides, the frequency of this resistant biotype is unlikely to increase in a population of mixed downy brome biotypes.

Downy brome is an introduced Mediterranean annual grass that now dominates millions of hectares of western U.S. rangelands. The presence of this grass has eliminated many native species and accelerated wildfire cycles. The objective of this study was to identify soil additives that allowed germination but inhibited emergence of downy brome, while not affecting germination or emergence of the native perennial grass Hilaria jamesii. On the basis of data from previous studies, we focused on additives that altered the availability of soil nitrogen (N), phosphorus (P), and potassium (K). Most water-soluble treatments inhibited downy brome germination and emergence. We attribute the inhibitory effects of these treatments to excessive salinity and ion-specific effects of the additives themselves. An exception to this was oxalic acid, which showed no effect. Most water-insoluble treatments had no effect in soils with high P but did have an effect in soils with low P. Zeolite was effective regardless of P level, probably due to the high amounts of Na+ it added to the soil solution. Most treatments at higher concentrations resulted in lower downy brome emergence rates in soils currently dominated by downy brome than in uninvaded (but theoretically invadable) Hilaria soils. This difference is possibly attributable to inherent differences in labile soil P. In Stipa soils, where Stipa spp. grow, but which are generally considered to be uninvadable by downy brome, additions of high amounts of N resulted in lower emergence. This may have been an effect of NH4 + interference with uptake of K or other cations or toxicity of high N. We also saw a positive relationship between downy brome emergence and pH in Stipa soils. Hilaria development parameters were not as susceptible to the treatments, regardless of concentration, as downy brome. Our results suggest that there are additions that may be effective management tools for inhibiting downy brome in calcareous soils, including (1) high salt applications, (2) K-reducing additions (e.g., Mg), and (3) P-reducing additions.

Studies quantifying weed seed production as a function of weed density are expensive and difficult, and lack of these data is a common limitation in modeling weed population dynamics over time. Observed empirical and theoretical relationships between crop yield loss curves and weed seed production curves led us to the hypothesis that there should be a strong relationship between the shapes of these two curves. Data from literature sources were evaluated to test this hypothesis for hyperbolic curves and to determine if the data describing the crop yield loss caused by weeds could provide estimates of the shape parameter of a hyperbolic equation for describing density dependence in weed reproduction. For each of 162 data sets, a shape parameter (N50) and a scale parameter (U) were estimated for an increasing hyperbolic model both for absolute crop yield loss as a function of weed density (N50YL, UYL) and for weed yield (either total biomass yield or seed yield) as a function of weed density (N50WY, UWY). N50YL was strongly correlated with N50WY across all data sets, with an apparent 1:1 relationship between the two. This relationship suggests that the shape parameter of the yield loss model may substitute for the shape parameter of a hyperbolic model describing the density-dependence of weed seed production. This substitution will be most useful in weed population modeling situations where data describing crop yield loss as a function of weed density are already available, but data describing weed seed production as a function of weed density are not available.

Producers in the semiarid Great Plains are exploring alternative crop rotations, with the goal of replacing winter wheat–fallow. In 1993, a study was established to compare performance of eight rotations comprised of various combinations with winter wheat (W), spring wheat (SW), dry pea (Pea), safflower (Saf), corn (C), sunflower (Sun), proso millet (M), or fallow (F). After 8 years, we characterized weed communities by recording seedling emergence in each rotation. Seventeen species were observed, with downy brome, kochia, horseweed, and stinkgrass comprising 87% of the community. Rotations with the least number of weed seedlings were W–F and SW–W–C–Sun; in comparison, weed density was six-fold higher in W–M. Density of downy brome and kochia was highest in W–M compared with other rotations, whereas stinkgrass and green foxtail were prominent in proso millet of the W–M and W–C–M rotations. Horseweed established readily in safflower and dry pea. In the semiarid Great Plains, designing rotations in a cycle of four that includes cool- and warm-season crops can be a key component of integrated weed management.

Downy brome, an obligately selfing winter annual, has invaded a variety of habitats in western North America. Seeds are at least conditionally dormant at dispersal in early summer and lose dormancy through dry after-ripening. In the field, patterns of germination response at dispersal vary among populations and across years within populations. Degree of dormancy at summer temperatures in recently harvested seeds, as well as rate of dormancy loss during dry storage, can be related to the risk of premature summer germination in different habitats. Patterns of dormancy loss are predictable and can be modeled using hydrothermal time concepts. To assess the relative contribution of genotype and maturation environment, multiple parental lines from contrasting populations were grown for three generations under manipulated greenhouse conditions. Significant germination response differences among populations were observed, as well as major differences among full-sib families. Among-population variation accounted for over 90% of the variance in germination traits, whereas within-family variance accounted for 1% or less. Populations from predictable extreme environments (subalpine meadow and warm desert margin) showed significantly less variation among families than did populations from less predictable environments (cold desert, foothill, and plains). Environmental conditions that shortened the seed ripening period (water stress and high temperature) resulted in reduced seed dormancy level at maturation, but there were strong inbred line–environment interactions. For fully after-ripened seeds, inbred line and environmental effects were no longer evident, indicating that differences in genotype and maturation environment function mainly to regulate dormancy and dormancy loss rather than to mediate response patterns of nondormant seeds.

Downy brome in dryland winter wheat presents a major constraint to the adoption of reduced tillage cropping systems in the Pacific Northwest of the United States. Effective suppression of downy brome during fallow periods depletes seed in the soil and reduces infestations in subsequent winter wheat crops. Delayed tillage operations or delayed herbicide applications in the spring increase the risk for production of viable downy brome seed during fallow periods. In a series of studies, downy brome panicles were sequentially sampled at Pendleton, OR, and Pullman, WA, in 1996 and 1997, and at nine locations around the winter wheat growing region of the western United States in 1999 and 2001. Cumulative growing degree days (GDD) were calculated using local, daily maximum, and minimum air temperature data. A simple GDD model based on the formula GDD = (daily maximum temperature [C] + daily minimum temperature [C])/2, with a base temperature of 0 C and a starting point of January 1, was used to calculate cumulative GDD values for panicle sampling dates. Number of seed germinating per collected panicle was recorded in greenhouse germination tests. Estimations of degree days required for production of viable downy brome seed were made using nonlinear regression of germination on GDD. The GDD value at which viable seed can be found on plants (i.e., when seed germination > 0) was of interest. Estimates of the GDD values at which viable seed could be found in the three studies ranged from 582 GDD at Bozeman, MT, to 1,287 GDD at Stillwater, OK, with a group of GDD values for Pendleton and Pullman around 1,000. Variation in seed-set GDD among locations may be attributed to differing climatic conditions that control vernalization at the various locations or to differences in vernalization requirements among downy brome biotypes (or both).