Livestock-associated MRSA has been found in various animals, livestock farmers and retail meat. This study aimed to determine the prevalence and determinants of nasal MRSA carriage in pig slaughterhouse workers. Three large pig slaughterhouses in The Netherlands were studied in 2008 using human and environmental samples. The overall prevalence of nasal MRSA carriage in employees of pig slaughterhouses was 5·6% (14/249) (95% CI 3·4–9·2) and working with live pigs was the single most important factor for being MRSA positive (OR 38·2, P<0·0001). At the start of the day MRSA was only found in environmental samples from the lairages (10/12), whereas at the end of the day MRSA was found in the lairages (11/12), the dirty (5/12) and clean (3/12) areas and green offal (1/3). The MRSA status of the environmental samples correlated well with the MRSA status of humans working in these sections (r=0·75). In conclusion, a high prevalence of nasal MRSA carriage was found in pig-slaughterhouse workers, and working with live pigs is the most important risk factor. Exact transmission routes from animals to humans remain to be elucidated in order to enable application of targeted preventive measures.

One hundred and twenty-seven groups of cattle were studied in a slaughterhouse lairage to determine the factors affecting their ability to rest. The cattle were studied by direct observation over one 30-min period during their first 3·5 h in the lairage. An ethogram/time budget analysis of one focal Hereford × Friesian steer or heifer per group was undertaken and the lying behaviour of the whole group was recorded. Linear regression analysis was used to examine the effect of length of time in the lairage on behaviour. The effects of source, sex, group size, space allowance and bedding were examined by a comparison of regression lines. In cattle from markets, standing and moving behaviour, and the number of aggressive interactions decreased and lying behaviour increased with time in the lairage. This was not the case in cattle coming direct from farms. In cattle from markets, steers settled down at a faster rate than heifers, and cattle kept in group sizes >5 settled down at a faster rate than those kept in group sizes ≤5. The presence of straw bedding was associated with greater lying behaviour in market heifers and in market cattle kept in group sizes ≤5 than in similar cattle without straw bedding. Cattle from markets but not those from farms started to settle down within 3·5 h of arrival in the lairage and their behaviour was affected by the social and physical environment.