In the description of German phonology, two distinct phonetic symbols are currently recommended for the transcription of the vowels [a] (a central low vowel, phonemically /a/) and [ɐ] (phonemically /əʁ/) in word-final, unstressed positions. The present study examines whether differences between these two vowels exist in production and perception of Standard German speakers from the north of Germany. In Experiment 1, six speakers produced a series of minimal pairs that were embedded in meaningful sentences and varied with respect to their accentuation and position within a prosodic phrase. In Experiment 2, the minimal pairs produced by the six speakers of the first experiment were extracted from their respective contexts and tested with 44 native German listeners in a forced-choice identification task. Perceptual results showed a better-than-chance performance for one male speaker of the corpus only. Phonetic analyses also confirmed that only this male speaker produced subtle, but consistent F2/F3 differences between [a] and [ɐ] while the contrast was completely neutralised in the rest of the corpus. We discuss the role of prosody in vowel neutralisation with a specific focus on unstressed vowels and make suggestions for phonetic and phonological accounts of Standard German.